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P40327 (PRS4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
26S protease regulatory subunit 4 homolog
Alternative name(s):
Tat-binding homolog 5
Gene names
Name:RPT2
Synonyms:YHS4, YTA5
Ordered Locus Names:YDL007W
ORF Names:D2920
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex By similarity. Has ATPase activity.

Subcellular location

Cytoplasm Potential. Nucleus Potential.

Sequence similarities

Belongs to the AAA ATPase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RPN1P387646EBI-13901,EBI-15913
RPT1P332997EBI-13901,EBI-13910

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 43743626S protease regulatory subunit 4 homolog
PRO_0000084685

Regions

Nucleotide binding223 – 2308ATP Potential

Amino acid modifications

Modified residue1761Phosphoserine Ref.7
Modified residue1781Phosphothreonine Ref.7
Modified residue1801Phosphoserine Ref.6 Ref.7
Modified residue1811Phosphotyrosine Ref.7
Modified residue1821Phosphoserine Ref.7
Modified residue3561Phosphoserine Ref.7
Modified residue3571Phosphothreonine Ref.7
Lipidation21N-myristoyl glycine Ref.5

Experimental info

Mutagenesis2291K → Q: 73% loss of ATPase activity. Ref.2
Sequence conflict3471K → N in AAA97498. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P40327 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: EB735354C3A0EBD4

FASTA43748,828
        10         20         30         40         50         60 
MGQGVSSGQD KKKKKGSNQK PKYEPPVQSK FGRKKRKGGP ATAEKLPNIY PSTRCKLKLL 

        70         80         90        100        110        120 
RMERIKDHLL LEEEFVSNSE ILKPFEKKQE EEKKQLEEIR GNPLSIGTLE EIIDDDHAIV 

       130        140        150        160        170        180 
TSPTMPDYYV SILSFVDKEL LEPGCSVLLH HKTMSIVGVL QDDADPMVSV MKMDKSPTES 

       190        200        210        220        230        240 
YSDIGGLESQ IQEIKESVEL PLTHPELYEE MGIKPPKGVI LYGAPGTGKT LLAKAVANQT 

       250        260        270        280        290        300 
SATFLRIVGS ELIQKYLGDG PRLCRQIFKV AGENAPSIVF IDEIDAIGTK RYDSNSGGER 

       310        320        330        340        350        360 
EIQRTMLELL NQLDGFDDRG DVKVIMATNK IETLDPALIR PGRIDRKILF ENPDLSTKKK 

       370        380        390        400        410        420 
ILGIHTSKMN LSEDVNLETL VTTKDDLSGA DIQAMCTEAG LLALRERRMQ VTAEDFKQAK 

       430 
ERVMKNKVEE NLEGLYL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
Yeast 10:1141-1155(1994) [PubMed: 7754704] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Cloning and expression of a yeast gene encoding a protein with ATPase activity and high identity to the subunit 4 of the human 26 S protease."
Lucero H.A., Chojnicki E.W.T., Mandiyan S., Nelson H., Nelson N.
J. Biol. Chem. 270:9178-9184(1995) [PubMed: 7721833] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LYS-229.
Strain: ATCC 200060 / W303.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome."
Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.
Arch. Biochem. Biophys. 409:341-348(2003) [PubMed: 12504901] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11, MYRISTOYLATION AT GLY-2.
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176; THR-178; SER-180; TYR-181; SER-182; SER-356 AND THR-357, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X81070 Genomic DNA. Translation: CAA56957.1.
L17040 Genomic DNA. Translation: AAA97498.1.
Z48432 Genomic DNA. Translation: CAA88352.1.
Z74055 Genomic DNA. Translation: CAA98563.1.
BK006938 Genomic DNA. Translation: DAA11841.1.
PIRS46613.
RefSeqNP_010277.1. NM_001180066.1.

3D structure databases

ProteinModelPortalP40327.
SMRP40327. Positions 87-163, 170-430.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6282N.
IntActP40327. 34 interactions.
MINTMINT-632695.
STRINGP40327.

Proteomic databases

PeptideAtlasP40327.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL007W; YDL007W; YDL007W.
GeneID851557.
KEGGsce:YDL007W.
NMPDRfig|4932.3.peg.1019.

Organism-specific databases

SGDS000002165. RPT2.

Phylogenomic databases

eggNOGfuNOG04533.
GeneTreeEFGT00050000000010.
HOGENOMHBG724153.
OMAFIRNQER.
OrthoDBEOG4FN7S6.

Gene expression databases

ArrayExpressP40327.
GenevestigatorP40327.
GermOnlineYDL007W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR005937. 26S_Psome_P45.
IPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
[Graphical view]
KOK03062.
PfamPF00004. AAA. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
TIGRFAMsTIGR01242. 26Sp45. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968983.

Entry information

Entry namePRS4_YEAST
AccessionPrimary (citable) accession number: P40327
Secondary accession number(s): D6VRY1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families