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Protein

26S proteasome regulatory subunit 4 homolog

Gene

RPT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). Has ATPase activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi223 – 230ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: SGD
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

  • nonfunctional rRNA decay Source: SGD
  • peptide catabolic process Source: SGD
  • positive regulation of protein catabolic process Source: SGD
  • positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: GO_Central
  • proteasome regulatory particle assembly Source: SGD
  • protein-containing complex localization Source: SGD
  • ubiquitin-dependent ERAD pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29438-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 4 homolog
Alternative name(s):
Tat-binding homolog 5
Gene namesi
Name:RPT2
Synonyms:YHS4, YTA5
Ordered Locus Names:YDL007W
ORF Names:D2920
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL007W
SGDiS000002165 RPT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi229K → Q: 73% loss of ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000846852 – 43726S proteasome regulatory subunit 4 homologAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Cross-linki234Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki255Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki290Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

MaxQBiP40327
PaxDbiP40327
PRIDEiP40327

PTM databases

iPTMnetiP40327

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32047, 333 interactors
DIPiDIP-6282N
IntActiP40327, 52 interactors
MINTiP40327
STRINGi4932.YDL007W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCOelectron microscopy4.80I1-437[»]
3JCPelectron microscopy4.60I1-437[»]
4CR2electron microscopy7.70I1-437[»]
4CR3electron microscopy9.30I1-437[»]
4CR4electron microscopy8.80I1-437[»]
5A5Belectron microscopy9.50I1-437[»]
5MP9electron microscopy4.10I1-437[»]
5MPAelectron microscopy4.50I1-437[»]
5MPBelectron microscopy7.80I1-437[»]
5MPCelectron microscopy7.70I1-437[»]
5WVIelectron microscopy6.30I1-437[»]
5WVKelectron microscopy4.20I1-437[»]
ProteinModelPortaliP40327
SMRiP40327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074818
HOGENOMiHOG000225143
InParanoidiP40327
KOiK03062
OMAiNATFIRV
OrthoDBiEOG092C19WS

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR035244 26S_subunit_4
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
PANTHERiPTHR23073:SF24 PTHR23073:SF24, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQGVSSGQD KKKKKGSNQK PKYEPPVQSK FGRKKRKGGP ATAEKLPNIY
60 70 80 90 100
PSTRCKLKLL RMERIKDHLL LEEEFVSNSE ILKPFEKKQE EEKKQLEEIR
110 120 130 140 150
GNPLSIGTLE EIIDDDHAIV TSPTMPDYYV SILSFVDKEL LEPGCSVLLH
160 170 180 190 200
HKTMSIVGVL QDDADPMVSV MKMDKSPTES YSDIGGLESQ IQEIKESVEL
210 220 230 240 250
PLTHPELYEE MGIKPPKGVI LYGAPGTGKT LLAKAVANQT SATFLRIVGS
260 270 280 290 300
ELIQKYLGDG PRLCRQIFKV AGENAPSIVF IDEIDAIGTK RYDSNSGGER
310 320 330 340 350
EIQRTMLELL NQLDGFDDRG DVKVIMATNK IETLDPALIR PGRIDRKILF
360 370 380 390 400
ENPDLSTKKK ILGIHTSKMN LSEDVNLETL VTTKDDLSGA DIQAMCTEAG
410 420 430
LLALRERRMQ VTAEDFKQAK ERVMKNKVEE NLEGLYL
Length:437
Mass (Da):48,828
Last modified:January 23, 2007 - v3
Checksum:iEB735354C3A0EBD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347K → N in AAA97498 (PubMed:7721833).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81070 Genomic DNA Translation: CAA56957.1
L17040 Genomic DNA Translation: AAA97498.1
Z48432 Genomic DNA Translation: CAA88352.1
Z74055 Genomic DNA Translation: CAA98563.1
BK006938 Genomic DNA Translation: DAA11841.1
PIRiS46613
RefSeqiNP_010277.1, NM_001180066.1

Genome annotation databases

EnsemblFungiiYDL007W; YDL007W; YDL007W
GeneIDi851557
KEGGisce:YDL007W

Similar proteinsi

Entry informationi

Entry nameiPRS4_YEAST
AccessioniPrimary (citable) accession number: P40327
Secondary accession number(s): D6VRY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health