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P40316 (SECU_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Securin
Gene names
Name:PDS1
Ordered Locus Names:YDR113C
ORF Names:YD9727.08C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1. Ref.6

Subunit structure

Interacts with the caspase-like ESP1, and prevents its protease activity probably by covering its active site. Interacts with CDC20. Ref.6 Ref.7

Subcellular location

Cytoplasm. Nucleus.

Domain

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway By similarity.

Post-translational modification

Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus. Ref.8

Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. Ref.5

Sequence similarities

Belongs to the securin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NUP84P528911EBI-16908,EBI-12337
SRP1Q028211EBI-16908,EBI-1797

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Securin
PRO_0000206366

Regions

Motif85 – 884D-box

Amino acid modifications

Modified residue1851Phosphoserine Ref.10
Modified residue1861Phosphoserine Ref.10
Modified residue2121Phosphoserine Ref.10
Modified residue2131Phosphoserine Ref.10
Modified residue2771Phosphoserine Ref.11
Modified residue2921Phosphoserine; by CDC28 Probable

Experimental info

Mutagenesis85 – 884RLPL → ALPA: Abolishes ubiquitination and subsequent degradation. Ref.5
Mutagenesis2771S → A: Affects phosphorylation and the interaction with ESP1. Ref.8
Mutagenesis2921S → A: Affects phosphorylation and the interaction with ESP1. Ref.8
Mutagenesis3041T → A: No effect. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P40316 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7B31777C539433F4

FASTA37341,838
        10         20         30         40         50         60 
MMPANEDKEN NIVYTGNESS GINFPQTPAH LLKRSHSNIL KPPVRLDQLK RDANSNNGNT 

        70         80         90        100        110        120 
LKYIQGGKEV SPTKRLHTHA QQQGRLPLAA KDNNRSKSFI FPETSNQSKD ADLPQLQNTL 

       130        140        150        160        170        180 
SIRKNDQLRK LSQISRSRSR ANHNDLLSNS RKLQKYGSVL GYNALPKMKS LVLKDLADSG 

       190        200        210        220        230        240 
KNEESSDDDE GNEDSESKLG KKLQSALLKQ DSSDGENELN GGLGLFNEQG GLQQLIKNST 

       250        260        270        280        290        300 
KNEQKTKNDK SDKTDDYDIE IAPQRQEPLP YVPEGYSPFQ QDDIEKLKTF NSPYKLDLED 

       310        320        330        340        350        360 
EDDTPDKVDL LPLEQIDEEG EKDETECITR NQEEGAALPL LSKNFKEVAA VPTMELVYSE 

       370 
EGLDPEELED LVT 

« Hide

References

« Hide 'large scale' references
[1]"Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)."
Yamamoto A., Guacci V., Koshland D.
J. Cell Biol. 133:99-110(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204626 / S288c / A364A.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p."
Cohen-Fix O., Peters J.M., Kirschner M.W., Koshland D.
Genes Dev. 10:3081-3093(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION, MUTAGENESIS OF 85-ARG--LEU-88.
[6]"An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast."
Ciosk R., Zachariae W., Michaelis C., Shevchenko A., Mann M., Nasmyth K.
Cell 93:1067-1076(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ESP1.
[7]"The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner."
Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O.
Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDC20.
[8]"Phosphorylation of the mitotic regulator Pds1/securin by Cdc28 is required for efficient nuclear localization of Esp1/separase."
Agarwal R., Cohen-Fix O.
Genes Dev. 16:1371-1382(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDC28, MUTAGENESIS OF SER-277; SER-292 AND THR-304.
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185; SER-186; SER-212 AND SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-277, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12185 Genomic DNA. Translation: AAB00106.1.
Z48758 Genomic DNA. Translation: CAA88666.1.
AY558573 Genomic DNA. Translation: AAS56899.1.
BK006938 Genomic DNA. Translation: DAA11958.1.
PIRS47911.
RefSeqNP_010398.3. NM_001180421.3.

3D structure databases

DisProtDP00256.
ProteinModelPortalP40316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32170. 118 interactions.
DIPDIP-2798N.
IntActP40316. 7 interactions.
MINTMINT-551568.
STRING4932.YDR113C.

Proteomic databases

PaxDbP40316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR113C; YDR113C; YDR113C.
GeneID851691.
KEGGsce:YDR113C.

Organism-specific databases

CYGDYDR113c.
SGDS000002520. PDS1.

Phylogenomic databases

eggNOGNOG39016.
HOGENOMHOG000154282.
KOK02627.
OMADCESANE.
OrthoDBEOG7ZGXG5.

Enzyme and pathway databases

BioCycYEAST:G3O-29713-MONOMER.

Gene expression databases

GenevestigatorP40316.

Family and domain databases

InterProIPR006940. Securin_separation_inhibitor.
[Graphical view]
PfamPF04856. Securin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969344.

Entry information

Entry nameSECU_YEAST
AccessionPrimary (citable) accession number: P40316
Secondary accession number(s): D6VS98
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families