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P40316

- SECU_YEAST

UniProt

P40316 - SECU_YEAST

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Protein

Securin

Gene

PDS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1.1 Publication

GO - Molecular functioni

  1. enzyme binding Source: SGD

GO - Biological processi

  1. meiosis I Source: SGD
  2. mitotic sister chromatid segregation Source: SGD
  3. protein localization Source: SGD
  4. recombinational repair Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29713-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Securin
Gene namesi
Name:PDS1
Ordered Locus Names:YDR113C
ORF Names:YD9727.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR113c.
SGDiS000002520. PDS1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
  3. spindle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 884RLPL → ALPA: Abolishes ubiquitination and subsequent degradation. 1 Publication
Mutagenesisi277 – 2771S → A: Affects phosphorylation and the interaction with ESP1. 1 Publication
Mutagenesisi292 – 2921S → A: Affects phosphorylation and the interaction with ESP1. 1 Publication
Mutagenesisi304 – 3041T → A: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373SecurinPRO_0000206366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei185 – 1851Phosphoserine1 Publication
Modified residuei186 – 1861Phosphoserine1 Publication
Modified residuei212 – 2121Phosphoserine1 Publication
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei277 – 2771Phosphoserine1 Publication
Modified residuei292 – 2921Phosphoserine; by CDC281 Publication

Post-translational modificationi

Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus.3 Publications
Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40316.
PaxDbiP40316.

Expressioni

Gene expression databases

GenevestigatoriP40316.

Interactioni

Subunit structurei

Interacts with the caspase-like ESP1, and prevents its protease activity probably by covering its active site. Interacts with CDC20.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP84P528911EBI-16908,EBI-12337
SRP1Q028211EBI-16908,EBI-1797

Protein-protein interaction databases

BioGridi32170. 119 interactions.
DIPiDIP-2798N.
IntActiP40316. 7 interactions.
MINTiMINT-551568.
STRINGi4932.YDR113C.

Structurei

3D structure databases

DisProtiDP00256.
ProteinModelPortaliP40316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi85 – 884D-box

Domaini

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity

Sequence similaritiesi

Belongs to the securin family.Curated

Phylogenomic databases

eggNOGiNOG39016.
HOGENOMiHOG000154282.
InParanoidiP40316.
KOiK02627.
OMAiDCESANE.
OrthoDBiEOG7ZGXG5.

Family and domain databases

InterProiIPR006940. Securin_separation_inhibitor.
[Graphical view]
PfamiPF04856. Securin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40316-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMPANEDKEN NIVYTGNESS GINFPQTPAH LLKRSHSNIL KPPVRLDQLK
60 70 80 90 100
RDANSNNGNT LKYIQGGKEV SPTKRLHTHA QQQGRLPLAA KDNNRSKSFI
110 120 130 140 150
FPETSNQSKD ADLPQLQNTL SIRKNDQLRK LSQISRSRSR ANHNDLLSNS
160 170 180 190 200
RKLQKYGSVL GYNALPKMKS LVLKDLADSG KNEESSDDDE GNEDSESKLG
210 220 230 240 250
KKLQSALLKQ DSSDGENELN GGLGLFNEQG GLQQLIKNST KNEQKTKNDK
260 270 280 290 300
SDKTDDYDIE IAPQRQEPLP YVPEGYSPFQ QDDIEKLKTF NSPYKLDLED
310 320 330 340 350
EDDTPDKVDL LPLEQIDEEG EKDETECITR NQEEGAALPL LSKNFKEVAA
360 370
VPTMELVYSE EGLDPEELED LVT
Length:373
Mass (Da):41,838
Last modified:February 1, 1995 - v1
Checksum:i7B31777C539433F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12185 Genomic DNA. Translation: AAB00106.1.
Z48758 Genomic DNA. Translation: CAA88666.1.
AY558573 Genomic DNA. Translation: AAS56899.1.
BK006938 Genomic DNA. Translation: DAA11958.1.
PIRiS47911.
RefSeqiNP_010398.3. NM_001180421.3.

Genome annotation databases

EnsemblFungiiYDR113C; YDR113C; YDR113C.
GeneIDi851691.
KEGGisce:YDR113C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12185 Genomic DNA. Translation: AAB00106.1 .
Z48758 Genomic DNA. Translation: CAA88666.1 .
AY558573 Genomic DNA. Translation: AAS56899.1 .
BK006938 Genomic DNA. Translation: DAA11958.1 .
PIRi S47911.
RefSeqi NP_010398.3. NM_001180421.3.

3D structure databases

DisProti DP00256.
ProteinModelPortali P40316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32170. 119 interactions.
DIPi DIP-2798N.
IntActi P40316. 7 interactions.
MINTi MINT-551568.
STRINGi 4932.YDR113C.

Proteomic databases

MaxQBi P40316.
PaxDbi P40316.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR113C ; YDR113C ; YDR113C .
GeneIDi 851691.
KEGGi sce:YDR113C.

Organism-specific databases

CYGDi YDR113c.
SGDi S000002520. PDS1.

Phylogenomic databases

eggNOGi NOG39016.
HOGENOMi HOG000154282.
InParanoidi P40316.
KOi K02627.
OMAi DCESANE.
OrthoDBi EOG7ZGXG5.

Enzyme and pathway databases

BioCyci YEAST:G3O-29713-MONOMER.

Miscellaneous databases

NextBioi 969344.

Gene expression databases

Genevestigatori P40316.

Family and domain databases

InterProi IPR006940. Securin_separation_inhibitor.
[Graphical view ]
Pfami PF04856. Securin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)."
    Yamamoto A., Guacci V., Koshland D.
    J. Cell Biol. 133:99-110(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p."
    Cohen-Fix O., Peters J.M., Kirschner M.W., Koshland D.
    Genes Dev. 10:3081-3093(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, MUTAGENESIS OF 85-ARG--LEU-88.
  6. "An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast."
    Ciosk R., Zachariae W., Michaelis C., Shevchenko A., Mann M., Nasmyth K.
    Cell 93:1067-1076(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ESP1.
  7. "The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner."
    Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O.
    Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC20.
  8. "Phosphorylation of the mitotic regulator Pds1/securin by Cdc28 is required for efficient nuclear localization of Esp1/separase."
    Agarwal R., Cohen-Fix O.
    Genes Dev. 16:1371-1382(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY CDC28, MUTAGENESIS OF SER-277; SER-292 AND THR-304.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185; SER-186; SER-212 AND SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-277, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSECU_YEAST
AccessioniPrimary (citable) accession number: P40316
Secondary accession number(s): D6VS98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3