P40316 (SECU_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Securin

Gene names

Name:	PDS1
Ordered Locus Names:	YDR113C
ORF Names:	YD9727.08C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1. Ref.6
Subunit structure	Interacts with the caspase-like ESP1, and prevents its protease activity probably by covering its active site. Interacts with CDC20. Ref.6 Ref.7
Subcellular location	Cytoplasm. Nucleus.
Domain	The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway By similarity.
Post-translational modification	Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus. Ref.8 Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. Ref.5
Sequence similarities	Belongs to the securin family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis I Inferred from mutant phenotype PubMed 19001291. Source: SGD mitotic sister chromatid segregation Inferred from physical interaction Ref.6. Source: SGD protein localization Inferred from mutant phenotype PubMed 11149918. Source: SGD recombinational repair Inferred from mutant phenotype PubMed 15533838. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 19520826. Source: SGD nucleus Inferred from direct assay PubMed 11149918PubMed 19520826. Source: SGD spindle Inferred from direct assay PubMed 11149918. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NUP84	P52891	1	EBI-16908,EBI-12337
SRP1	Q02821	1	EBI-16908,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Securin		PRO_0000206366
Regions
	Motif	85 – 88	4	D-box
Amino acid modifications
	Modified residue	37	1	Phosphoserine Ref.9
	Modified residue	139	1	Phosphoserine Ref.9
	Modified residue	179	1	Phosphoserine Ref.11
	Modified residue	185	1	Phosphoserine Ref.10 Ref.11
	Modified residue	186	1	Phosphoserine Ref.10 Ref.11
	Modified residue	212	1	Phosphoserine Ref.11
	Modified residue	213	1	Phosphoserine Ref.11
	Modified residue	277	1	Phosphoserine Ref.10
	Modified residue	292	1	Phosphoserine; by CDC28 Probable
Experimental info
	Mutagenesis	85 – 88	4	RLPL → ALPA: Abolishes ubiquitination and subsequent degradation. Ref.5
	Mutagenesis	277	1	S → A: Affects phosphorylation and the interaction with ESP1. Ref.8
	Mutagenesis	292	1	S → A: Affects phosphorylation and the interaction with ESP1. Ref.8
	Mutagenesis	304	1	T → A: No effect. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P40316 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7B31777C539433F4	FASTA	373	41,838
	10 20 30 40 50 60 MMPANEDKEN NIVYTGNESS GINFPQTPAH LLKRSHSNIL KPPVRLDQLK RDANSNNGNT 70 80 90 100 110 120 LKYIQGGKEV SPTKRLHTHA QQQGRLPLAA KDNNRSKSFI FPETSNQSKD ADLPQLQNTL 130 140 150 160 170 180 SIRKNDQLRK LSQISRSRSR ANHNDLLSNS RKLQKYGSVL GYNALPKMKS LVLKDLADSG 190 200 210 220 230 240 KNEESSDDDE GNEDSESKLG KKLQSALLKQ DSSDGENELN GGLGLFNEQG GLQQLIKNST 250 260 270 280 290 300 KNEQKTKNDK SDKTDDYDIE IAPQRQEPLP YVPEGYSPFQ QDDIEKLKTF NSPYKLDLED 310 320 330 340 350 360 EDDTPDKVDL LPLEQIDEEG EKDETECITR NQEEGAALPL LSKNFKEVAA VPTMELVYSE 370 EGLDPEELED LVT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)." Yamamoto A., Guacci V., Koshland D. J. Cell Biol. 133:99-110(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1678.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p." Cohen-Fix O., Peters J.M., Kirschner M.W., Koshland D. Genes Dev. 10:3081-3093(1996) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION, MUTAGENESIS OF 85-ARG--LEU-88.
[6]	"An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast." Ciosk R., Zachariae W., Michaelis C., Shevchenko A., Mann M., Nasmyth K. Cell 93:1067-1076(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ESP1.
[7]	"The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner." Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O. Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDC20.
[8]	"Phosphorylation of the mitotic regulator Pds1/securin by Cdc28 is required for efficient nuclear localization of Esp1/separase." Agarwal R., Cohen-Fix O. Genes Dev. 16:1371-1382(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY CDC28, MUTAGENESIS OF SER-277; SER-292 AND THR-304.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-139, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185; SER-186 AND SER-277, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179; SER-185; SER-186; SER-212 AND SER-213, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12185 Genomic DNA. Translation: AAB00106.1. Z48758 Genomic DNA. Translation: CAA88666.1. AY558573 Genomic DNA. Translation: AAS56899.1. BK006938 Genomic DNA. Translation: DAA11958.1.
PIR	S47911.
RefSeq	NP_010398.3. NM_001180421.3.
3D structure databases
DisProt	DP00256.
ProteinModelPortal	P40316.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2798N.
IntAct	P40316. 8 interactions.
MINT	MINT-551568.
STRING	4932.YDR113C.
Proteomic databases
PaxDb	P40316.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR113C; YDR113C; YDR113C.
GeneID	851691.
KEGG	sce:YDR113C. sce:YDR117C.
Organism-specific databases
CYGD	YDR113c.
SGD	S000002520. PDS1.
Phylogenomic databases
eggNOG	NOG39016.
HOGENOM	HOG000154282.
KO	K02627. K15027.
OMA	DCESANE.
OrthoDB	EOG4TTKTS.
Gene expression databases
Genevestigator	P40316.
GermOnline	YDR113C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006940. Securin_separation_inhibitor. [Graphical view]
Pfam	PF04856. Securin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969344.

Entry information

Entry name

SECU_YEAST

Accession

Primary (citable) accession number: P40316
Secondary accession number(s): D6VS98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program