Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Securin

Gene

PDS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1.1 Publication

GO - Molecular functioni

  • enzyme binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: SGD
  • meiosis I Source: SGD
  • mitotic sister chromatid segregation Source: SGD
  • negative regulation of exit from mitosis Source: SGD
  • protein localization Source: SGD
  • recombinational repair Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29713-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Securin
Gene namesi
Name:PDS1
Ordered Locus Names:YDR113C
ORF Names:YD9727.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR113C
SGDiS000002520 PDS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85 – 88RLPL → ALPA: Abolishes ubiquitination and subsequent degradation. 1 Publication4
Mutagenesisi277S → A: Affects phosphorylation and the interaction with ESP1. 1 Publication1
Mutagenesisi292S → A: Affects phosphorylation and the interaction with ESP1. 1 Publication1
Mutagenesisi304T → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002063661 – 373SecurinAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei185PhosphoserineCombined sources1
Modified residuei186PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Modified residuei277PhosphoserineCombined sources1 Publication1
Modified residuei292Phosphoserine; by CDC281 Publication1

Post-translational modificationi

Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus.1 Publication
Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40316
PaxDbiP40316
PRIDEiP40316

PTM databases

iPTMnetiP40316

Interactioni

Subunit structurei

Interacts with the caspase-like ESP1, and prevents its protease activity probably by covering its active site. Interacts with CDC20.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ESP1Q030182EBI-16908,EBI-6657

GO - Molecular functioni

  • enzyme binding Source: SGD

Protein-protein interaction databases

BioGridi32170, 210 interactors
DIPiDIP-2798N
IntActiP40316, 13 interactors
MINTiP40316
STRINGi4932.YDR113C

Structurei

Secondary structure

1373
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi273 – 275Combined sources3
Helixi281 – 288Combined sources8
Helixi365 – 371Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5U1SX-ray3.00B258-373[»]
5U1TX-ray2.60B258-373[»]
DisProtiDP00256
ProteinModelPortaliP40316
SMRiP40316
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi85 – 88D-box4

Domaini

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity

Sequence similaritiesi

Belongs to the securin family.Curated

Phylogenomic databases

HOGENOMiHOG000154282
InParanoidiP40316
KOiK02627
OMAiDCESANE
OrthoDBiEOG092C3LC0

Family and domain databases

InterProiView protein in InterPro
IPR006940 Securin_separation_inhibitor
PfamiView protein in Pfam
PF04856 Securin, 1 hit

Sequencei

Sequence statusi: Complete.

P40316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMPANEDKEN NIVYTGNESS GINFPQTPAH LLKRSHSNIL KPPVRLDQLK
60 70 80 90 100
RDANSNNGNT LKYIQGGKEV SPTKRLHTHA QQQGRLPLAA KDNNRSKSFI
110 120 130 140 150
FPETSNQSKD ADLPQLQNTL SIRKNDQLRK LSQISRSRSR ANHNDLLSNS
160 170 180 190 200
RKLQKYGSVL GYNALPKMKS LVLKDLADSG KNEESSDDDE GNEDSESKLG
210 220 230 240 250
KKLQSALLKQ DSSDGENELN GGLGLFNEQG GLQQLIKNST KNEQKTKNDK
260 270 280 290 300
SDKTDDYDIE IAPQRQEPLP YVPEGYSPFQ QDDIEKLKTF NSPYKLDLED
310 320 330 340 350
EDDTPDKVDL LPLEQIDEEG EKDETECITR NQEEGAALPL LSKNFKEVAA
360 370
VPTMELVYSE EGLDPEELED LVT
Length:373
Mass (Da):41,838
Last modified:February 1, 1995 - v1
Checksum:i7B31777C539433F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12185 Genomic DNA Translation: AAB00106.1
Z48758 Genomic DNA Translation: CAA88666.1
AY558573 Genomic DNA Translation: AAS56899.1
BK006938 Genomic DNA Translation: DAA11958.1
PIRiS47911
RefSeqiNP_010398.3, NM_001180421.3

Genome annotation databases

EnsemblFungiiYDR113C; YDR113C; YDR113C
GeneIDi851691
KEGGisce:YDR113C

Similar proteinsi

Entry informationi

Entry nameiSECU_YEAST
AccessioniPrimary (citable) accession number: P40316
Secondary accession number(s): D6VS98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health