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Protein

Nascent polypeptide-associated complex subunit beta-2

Gene

BTT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the nascent polypeptide-associated complex (NAC), which promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane. Also blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. BTT1 may act as a transcription factor that exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' cotranslational protein folding Source: SGD
  • posttranslational protein targeting to membrane Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29824-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit beta-2
Short name:
NAC-beta-2
Alternative name(s):
BTF3 homolog BTT1
Beta-2-NAC
Gene namesi
Name:BTT1
Ordered Locus Names:YDR252W
ORF Names:YD9320A.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR252W.
SGDiS000002660. BTT1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Predominantly cytoplasmic, may also transiently localize to the nucleus.By similarity

GO - Cellular componenti

  • CCR4-NOT core complex Source: SGD
  • nascent polypeptide-associated complex Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Nascent polypeptide-associated complex subunit beta-2PRO_0000213551Add
BLAST

Proteomic databases

MaxQBiP40314.
PeptideAtlasiP40314.

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and either EGD1 or BTT1. NAC associates with ribosomes via EGD1 or BTT1.

Binary interactionsi

WithEntry#Exp.IntActNotes
RPL25P044563EBI-3802,EBI-15308

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi32302. 28 interactions.
DIPiDIP-2099N.
IntActiP40314. 5 interactions.
MINTiMINT-537256.

Structurei

3D structure databases

ProteinModelPortaliP40314.
SMRiP40314. Positions 53-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 10366NAC-A/BPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NAC-beta family.Curated
Contains 1 NAC-A/B (NAC-alpha/beta) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
InParanoidiP40314.
OMAiHNLENEQ.
OrthoDBiEOG780S01.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVDQEKLAK LHKLSAANKV GGTRRKINKK GNLYNNNDKD NTKLQAELHK
60 70 80 90 100
LHPMTIENVA EANFFKKNGK VLHFNSAVVQ IAPQCNLTMI HGQPKENTLN
110 120 130 140
GLYPSVASQL GSQELEYLTG LAHNLENEQT VLDQLGDRCS ETKQQVMNS
Length:149
Mass (Da):16,620
Last modified:February 1, 1995 - v1
Checksum:i5EED697F1E728D09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78724 Genomic DNA. Translation: CAA55370.1.
Z70202 Genomic DNA. Translation: CAA94091.1.
Z68329 Genomic DNA. Translation: CAA92709.1.
AY557726 Genomic DNA. Translation: AAS56052.1.
BK006938 Genomic DNA. Translation: DAA12092.1.
PIRiS47574.
RefSeqiNP_010538.1. NM_001180560.1.

Genome annotation databases

EnsemblFungiiYDR252W; YDR252W; YDR252W.
GeneIDi851839.
KEGGisce:YDR252W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78724 Genomic DNA. Translation: CAA55370.1.
Z70202 Genomic DNA. Translation: CAA94091.1.
Z68329 Genomic DNA. Translation: CAA92709.1.
AY557726 Genomic DNA. Translation: AAS56052.1.
BK006938 Genomic DNA. Translation: DAA12092.1.
PIRiS47574.
RefSeqiNP_010538.1. NM_001180560.1.

3D structure databases

ProteinModelPortaliP40314.
SMRiP40314. Positions 53-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32302. 28 interactions.
DIPiDIP-2099N.
IntActiP40314. 5 interactions.
MINTiMINT-537256.

Proteomic databases

MaxQBiP40314.
PeptideAtlasiP40314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR252W; YDR252W; YDR252W.
GeneIDi851839.
KEGGisce:YDR252W.

Organism-specific databases

EuPathDBiFungiDB:YDR252W.
SGDiS000002660. BTT1.

Phylogenomic databases

GeneTreeiENSGT00390000006283.
HOGENOMiHOG000261245.
InParanoidiP40314.
OMAiHNLENEQ.
OrthoDBiEOG780S01.

Enzyme and pathway databases

BioCyciYEAST:G3O-29824-MONOMER.

Miscellaneous databases

PROiP40314.

Family and domain databases

InterProiIPR002715. Nas_poly-pep-assoc_cplx_dom.
[Graphical view]
PfamiPF01849. NAC. 1 hit.
[Graphical view]
SMARTiSM01407. NAC. 1 hit.
[Graphical view]
PROSITEiPS51151. NAC_AB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast BTF3 protein is encoded by duplicated genes and inhibits the expression of some genes in vivo."
    Hu G.-Z., Ronne H.
    Nucleic Acids Res. 22:2740-2743(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208353 / W303-1A.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Initial characterization of the nascent polypeptide-associated complex in yeast."
    Reimann B., Bradsher J., Franke J., Hartmann E., Wiedmann M., Prehn S., Wiedmann B.
    Yeast 15:397-407(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EGD2 AND RIBOSOMES.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNACB2_YEAST
AccessioniPrimary (citable) accession number: P40314
Secondary accession number(s): D6VSN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.