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Protein

Nascent polypeptide-associated complex subunit beta-2

Gene

BTT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the nascent polypeptide-associated complex (NAC), which promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane. Also blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. BTT1 may act as a transcription factor that exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II.1 Publication

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' cotranslational protein folding Source: SGD
  • posttranslational protein targeting to endoplasmic reticulum membrane Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29824-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nascent polypeptide-associated complex subunit beta-2
Short name:
NAC-beta-2
Alternative name(s):
BTF3 homolog BTT1
Beta-2-NAC
Gene namesi
Name:BTT1
Ordered Locus Names:YDR252W
ORF Names:YD9320A.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR252W
SGDiS000002660 BTT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135511 – 149Nascent polypeptide-associated complex subunit beta-2Add BLAST149

Proteomic databases

MaxQBiP40314
PaxDbiP40314
PRIDEiP40314

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and either EGD1 or BTT1. NAC associates with ribosomes via EGD1 or BTT1.

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi32302, 88 interactors
DIPiDIP-2099N
IntActiP40314, 2 interactors
MINTiP40314
STRINGi4932.YDR252W

Structurei

3D structure databases

ProteinModelPortaliP40314
SMRiP40314
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 103NAC-A/BPROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the NAC-beta family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006283
HOGENOMiHOG000261245
InParanoidiP40314
OrthoDBiEOG092C58TX

Family and domain databases

Gene3Di2.20.70.30, 1 hit
InterProiView protein in InterPro
IPR038187 NAC_A/B_dom_sf
IPR002715 Nas_poly-pep-assoc_cplx_dom
PfamiView protein in Pfam
PF01849 NAC, 1 hit
SMARTiView protein in SMART
SM01407 NAC, 1 hit
PROSITEiView protein in PROSITE
PS51151 NAC_AB, 1 hit

Sequencei

Sequence statusi: Complete.

P40314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVDQEKLAK LHKLSAANKV GGTRRKINKK GNLYNNNDKD NTKLQAELHK
60 70 80 90 100
LHPMTIENVA EANFFKKNGK VLHFNSAVVQ IAPQCNLTMI HGQPKENTLN
110 120 130 140
GLYPSVASQL GSQELEYLTG LAHNLENEQT VLDQLGDRCS ETKQQVMNS
Length:149
Mass (Da):16,620
Last modified:February 1, 1995 - v1
Checksum:i5EED697F1E728D09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78724 Genomic DNA Translation: CAA55370.1
Z70202 Genomic DNA Translation: CAA94091.1
Z68329 Genomic DNA Translation: CAA92709.1
AY557726 Genomic DNA Translation: AAS56052.1
BK006938 Genomic DNA Translation: DAA12092.1
PIRiS47574
RefSeqiNP_010538.1, NM_001180560.1

Genome annotation databases

EnsemblFungiiYDR252W; YDR252W; YDR252W
GeneIDi851839
KEGGisce:YDR252W

Entry informationi

Entry nameiNACB2_YEAST
AccessioniPrimary (citable) accession number: P40314
Secondary accession number(s): D6VSN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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