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Protein

Cytochrome b5

Gene

CYB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It plays a role in fatty-acid desaturation and is also involved in several steps of the sterol biosynthesis pathway, particularly in the 4-demethylation of the 4,4'-dimethyl zymosterol.

Miscellaneous

Present with 5390 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi61Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • electron transfer activity Source: SGD
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • sterol biosynthetic process Source: SGD

Keywordsi

Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33135-MONOMER
ReactomeiR-SCE-196836 Vitamin C (ascorbate) metabolism
SABIO-RKP40312

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5
Ordered Locus Names:YNL111C
ORF Names:N1949
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL111C
SGDiS000005055 CYB5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660351 – 120Cytochrome b5Add BLAST120

Proteomic databases

MaxQBiP40312
PaxDbiP40312
PRIDEiP40312
TopDownProteomicsiP40312

Interactioni

Protein-protein interaction databases

BioGridi35714, 161 interactors
IntActiP40312, 4 interactors
MINTiP40312
STRINGi4932.YNL111C

Structurei

3D structure databases

ProteinModelPortaliP40312
SMRiP40312
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 78Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046507
HOGENOMiHOG000039853
InParanoidiP40312
OMAiEYISHAK
OrthoDBiEOG092C43G8

Family and domain databases

Gene3Di3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PRINTSiPR00363 CYTOCHROMEB5
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit

Sequencei

Sequence statusi: Complete.

P40312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKVYSYQEV AEHNGPENFW IIIDDKVYDV SQFKDEHPGG DEIIMDLGGQ
60 70 80 90 100
DATESFVDIG HSDEALRLLK GLYIGDVDKT SERVSVEKVS TSENQSKGSG
110 120
TLVVILAILM LGVAYYLLNE
Length:120
Mass (Da):13,297
Last modified:October 1, 1996 - v2
Checksum:i598EF2A6730CAD19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17E → Q in AAA67468 (PubMed:8181746).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22494 Genomic DNA Translation: AAA67468.1
Z69382 Genomic DNA Translation: CAA93396.1
Z71387 Genomic DNA Translation: CAA95990.1
AY693106 Genomic DNA Translation: AAT93125.1
BK006947 Genomic DNA Translation: DAA10436.1
PIRiS63052
RefSeqiNP_014288.3, NM_001182949.3

Genome annotation databases

EnsemblFungiiYNL111C; YNL111C; YNL111C
GeneIDi855612
KEGGisce:YNL111C

Similar proteinsi

Entry informationi

Entry nameiCYB5_YEAST
AccessioniPrimary (citable) accession number: P40312
Secondary accession number(s): D6W170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health