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Protein

Cytochrome b5

Gene

CYB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It plays a role in fatty-acid desaturation and is also involved in several steps of the sterol biosynthesis pathway, particularly in the 4-demethylation of the 4,4'-dimethyl zymosterol.

Miscellaneous

Present with 5390 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi61Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

  • electron carrier activity Source: SGD
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB-KW
  • sterol biosynthetic process Source: SGD

Keywordsi

Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33135-MONOMER.
ReactomeiR-SCE-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5
Ordered Locus Names:YNL111C
ORF Names:N1949
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL111C.
SGDiS000005055. CYB5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660351 – 120Cytochrome b5Add BLAST120

Proteomic databases

MaxQBiP40312.
PRIDEiP40312.
TopDownProteomicsiP40312.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC155Q8N6L03EBI-2095239,EBI-749265From Homo sapiens.

Protein-protein interaction databases

BioGridi35714. 158 interactors.
IntActiP40312. 6 interactors.
MINTiMINT-2788942.
STRINGi4932.YNL111C.

Structurei

3D structure databases

ProteinModelPortaliP40312.
SMRiP40312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 78Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
InParanoidiP40312.
OMAiDAREMMD.
OrthoDBiEOG092C43G8.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiView protein in InterPro
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
PfamiView protein in Pfam
PF00173. Cyt-b5. 1 hit.
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiView protein in SMART
SM01117. Cyt-b5. 1 hit.
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiView protein in PROSITE
PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P40312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKVYSYQEV AEHNGPENFW IIIDDKVYDV SQFKDEHPGG DEIIMDLGGQ
60 70 80 90 100
DATESFVDIG HSDEALRLLK GLYIGDVDKT SERVSVEKVS TSENQSKGSG
110 120
TLVVILAILM LGVAYYLLNE
Length:120
Mass (Da):13,297
Last modified:October 1, 1996 - v2
Checksum:i598EF2A6730CAD19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17E → Q in AAA67468 (PubMed:8181746).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22494 Genomic DNA. Translation: AAA67468.1.
Z69382 Genomic DNA. Translation: CAA93396.1.
Z71387 Genomic DNA. Translation: CAA95990.1.
AY693106 Genomic DNA. Translation: AAT93125.1.
BK006947 Genomic DNA. Translation: DAA10436.1.
PIRiS63052.
RefSeqiNP_014288.3. NM_001182949.3.

Genome annotation databases

EnsemblFungiiYNL111C; YNL111C; YNL111C.
GeneIDi855612.
KEGGisce:YNL111C.

Similar proteinsi

Entry informationi

Entry nameiCYB5_YEAST
AccessioniPrimary (citable) accession number: P40312
Secondary accession number(s): D6W170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names