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Protein

Cytochrome b5

Gene

CYB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It plays a role in fatty-acid desaturation and is also involved in several steps of the sterol biosynthesis pathway, particularly in the 4-demethylation of the 4,4'-dimethyl zymosterol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi61 – 611Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  • electron carrier activity Source: SGD
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB-KW
  • sterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33135-MONOMER.
ReactomeiR-SCE-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:CYB5
Ordered Locus Names:YNL111C
ORF Names:N1949
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL111C.
SGDiS000005055. CYB5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Cytochrome b5PRO_0000166035Add
BLAST

Proteomic databases

MaxQBiP40312.
TopDownProteomicsiP40312.

Interactioni

Protein-protein interaction databases

BioGridi35714. 65 interactions.
IntActiP40312. 4 interactions.
MINTiMINT-2788942.

Structurei

3D structure databases

ProteinModelPortaliP40312.
SMRiP40312. Positions 5-77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7877Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
InParanoidiP40312.
OMAiFEDVGHT.
OrthoDBiEOG092C43G8.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKVYSYQEV AEHNGPENFW IIIDDKVYDV SQFKDEHPGG DEIIMDLGGQ
60 70 80 90 100
DATESFVDIG HSDEALRLLK GLYIGDVDKT SERVSVEKVS TSENQSKGSG
110 120
TLVVILAILM LGVAYYLLNE
Length:120
Mass (Da):13,297
Last modified:October 1, 1996 - v2
Checksum:i598EF2A6730CAD19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171E → Q in AAA67468 (PubMed:8181746).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22494 Genomic DNA. Translation: AAA67468.1.
Z69382 Genomic DNA. Translation: CAA93396.1.
Z71387 Genomic DNA. Translation: CAA95990.1.
AY693106 Genomic DNA. Translation: AAT93125.1.
BK006947 Genomic DNA. Translation: DAA10436.1.
PIRiS63052.
RefSeqiNP_014288.3. NM_001182949.3.

Genome annotation databases

EnsemblFungiiYNL111C; YNL111C; YNL111C.
GeneIDi855612.
KEGGisce:YNL111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22494 Genomic DNA. Translation: AAA67468.1.
Z69382 Genomic DNA. Translation: CAA93396.1.
Z71387 Genomic DNA. Translation: CAA95990.1.
AY693106 Genomic DNA. Translation: AAT93125.1.
BK006947 Genomic DNA. Translation: DAA10436.1.
PIRiS63052.
RefSeqiNP_014288.3. NM_001182949.3.

3D structure databases

ProteinModelPortaliP40312.
SMRiP40312. Positions 5-77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35714. 65 interactions.
IntActiP40312. 4 interactions.
MINTiMINT-2788942.

Proteomic databases

MaxQBiP40312.
TopDownProteomicsiP40312.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL111C; YNL111C; YNL111C.
GeneIDi855612.
KEGGisce:YNL111C.

Organism-specific databases

EuPathDBiFungiDB:YNL111C.
SGDiS000005055. CYB5.

Phylogenomic databases

GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
InParanoidiP40312.
OMAiFEDVGHT.
OrthoDBiEOG092C43G8.

Enzyme and pathway databases

BioCyciYEAST:G3O-33135-MONOMER.
ReactomeiR-SCE-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

PROiP40312.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5_YEAST
AccessioniPrimary (citable) accession number: P40312
Secondary accession number(s): D6W170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5390 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.