K(+)/H(+) antiporter 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40309 (KHA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
K(+)/H(+) antiporter 1

Gene names

Name:	KHA1
Ordered Locus Names:	YJL094C
ORF Names:	J0909

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potassium-proton antiport.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 172 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. [View classification]

Ontologies

Keywords
Biological process	Antiport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 15926887. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	potassium:hydrogen antiporter activity Inferred from mutant phenotype PubMed 9811642. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADH1	P00330	1	EBI-25993,EBI-2218

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 873

873

K(+)/H(+) antiporter 1

PRO_0000196618

Regions

Topological domain

1 – 23

Extracellular Potential

Transmembrane

24 – 44

Helical; Potential

Topological domain

45 – 51

Cytoplasmic Potential

Transmembrane

52 – 72

Helical; Potential

Topological domain

73 – 82

Extracellular Potential

Transmembrane

83 – 103

Helical; Potential

Topological domain

104 – 116

Cytoplasmic Potential

Transmembrane

117 – 137

Helical; Potential

Topological domain

138 – 154

Extracellular Potential

Transmembrane

155 – 175

Helical; Potential

Topological domain

176 – 188

Cytoplasmic Potential

Transmembrane

189 – 209

Helical; Potential

Topological domain

210 – 220

Extracellular Potential

Transmembrane

221 – 241

Helical; Potential

Topological domain

242 – 267

Cytoplasmic Potential

Transmembrane

268 – 288

Helical; Potential

Topological domain

289 – 316

Extracellular Potential

Transmembrane

317 – 337

Helical; Potential

Topological domain

338 – 341

Cytoplasmic Potential

Transmembrane

342 – 362

Helical; Potential

Topological domain

363 – 375

Extracellular Potential

Transmembrane

376 – 396

Helical; Potential

Topological domain

397 – 404

Cytoplasmic Potential

Transmembrane

405 – 425

Helical; Potential

Topological domain

426 – 726

301

Extracellular Potential

Transmembrane

727 – 747

Helical; Potential

Topological domain

748 – 873

126

Cytoplasmic Potential

Amino acid modifications

Modified residue

557

Phosphoserine Ref.6 Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P40309 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 881E55A1F59C8CE6
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FASTA

873

97,111

        10         20         30         40         50         60 
MANTVGGILS GVNPFHYNSS SPLTLFLFQA CLILLVCNLI HIPFSMMRQP KVISEVISGV 

        70         80         90        100        110        120 
ILGPTIFGQI PNYTNTIFPT SSIPGLNLVA NLGIILFMFF LGLEVDIAFI KKHLKKALVI 

       130        140        150        160        170        180 
GIVTLAVPFG FGCLLAIPLF HTYANKTEGE RHIKFSVFMV FIAVSISVTA FPVLCRILNE 

       190        200        210        220        230        240 
LRLIKDRAGI VVLAAGIIND IMGWILLALS IILSSAEGSP VNTVYILLIT FAWFLIYFFP 

       250        260        270        280        290        300 
LKYLLRWVLI RTHELDRSKP SPLATMCILF IMFISAYFTD IIGVHPIFGA FIAGLVVPRD 

       310        320        330        340        350        360 
DHYVVKLTER MEDIPNIVFI PIYFAVAGLN VDLTLLNEGR DWGYVFATIG IAIFTKIISG 

       370        380        390        400        410        420 
TLTAKLTGLF WREATAAGVL MSCKGIVEIV VLTVGLNAGI ISRKIFGMFV LMALVSTFVT 

       430        440        450        460        470        480 
TPLTQLVYPD SYRDGVRKSL STPAEDDGAA DGLDSEGVDK TEINTQLNSL ADVSKYRIGE 

       490        500        510        520        530        540 
LTTVINTTEA ISPSLKLLNY LSLGVSPKPK NNKHKNETSL SRMTTATDST LKSNTFKIKK 

       550        560        570        580        590        600 
MVHIWSKSVD DVDTNLSVID EKLTPFEGVG ALRAIHLRLL TERTTDLLQS SSLYNDDPHF 

       610        620        630        640        650        660 
TANTDSLLQI FDIFSNLSKI PFSSEVIFST MREKAANIAT MKMDSTDLIL LPLKGASYEY 

       670        680        690        700        710        720 
RGSPVFIDEK YANFDHIYSH LLGLNELSST FFKSIFQSLK ANFAVQISNT YGRLNADRFK 

       730        740        750        760        770        780 
RKRFNLLLPK PYLTQSDYLG LYLLLLICYR DGYNNDNASC SIFINSKNID FAKDLSTAFA 

       790        800        810        820        830        840 
EHDWLNESTI KIVDIPFETK VPEEAIEKPS FIETVLDVGL SDTALADIEE TTFIIGEDLP 

       850        860        870 
DESEPFSEEV RTVIFEGSNR RFDTLIVHHF SSE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X." Miosga T., Witzel A., Zimmermann F.K. Yeast 10:965-973(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, MASS SPECTROMETRY.
[8]	"Sites of ubiquitin attachment in Saccharomyces cerevisiae." Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S. Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77087 Genomic DNA. Translation: CAA54359.1. Z49369 Genomic DNA. Translation: CAA89387.1. BK006943 Genomic DNA. Translation: DAA08706.1.
PIR	S46584.
RefSeq	NP_012441.1. NM_001181527.1.
3D structure databases
ProteinModelPortal	P40309.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4799N.
IntAct	P40309. 1 interaction.
MINT	MINT-494478.
STRING	4932.YJL094C.
Protein family/group databases
TCDB	2.A.37.4.1. the monovalent cation:proton antiporter-2 (cpa2) family.
Proteomic databases
PaxDb	P40309.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL094C; YJL094C; YJL094C.
GeneID	853351.
KEGG	sce:YJL094C.
Organism-specific databases
CYGD	YJL094c.
SGD	S000003630. KHA1.
Phylogenomic databases
eggNOG	COG0475.
HOGENOM	HOG000247916.
OMA	TERMEDI.
OrthoDB	EOG7GBG5B.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31549-MONOMER.
Gene expression databases
Genevestigator	P40309.
Family and domain databases
InterPro	IPR006153. Cation/H_exchanger. IPR004771. K/H_exchanger. [Graphical view]
Pfam	PF00999. Na_H_Exchanger. 1 hit. [Graphical view]
TIGRFAMs	TIGR00932. 2a37. 1 hit.
ProtoNet	Search...
Other
NextBio	973756.

Entry information

Entry name

KHA1_YEAST

Accession

Primary (citable) accession number: P40309
Secondary accession number(s): D6VW90

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 13, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents