Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P40309 (KHA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
K(+)/H(+) antiporter 1
Gene names
Name:KHA1
Ordered Locus Names:YJL094C
ORF Names:J0909
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potassium-proton antiport.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. [View classification]

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ADH1P003301EBI-25993,EBI-2218

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873K(+)/H(+) antiporter 1
PRO_0000196618

Regions

Topological domain1 – 2323Extracellular Potential
Transmembrane24 – 4421Helical; Potential
Topological domain45 – 517Cytoplasmic Potential
Transmembrane52 – 7221Helical; Potential
Topological domain73 – 8210Extracellular Potential
Transmembrane83 – 10321Helical; Potential
Topological domain104 – 11613Cytoplasmic Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 15417Extracellular Potential
Transmembrane155 – 17521Helical; Potential
Topological domain176 – 18813Cytoplasmic Potential
Transmembrane189 – 20921Helical; Potential
Topological domain210 – 22011Extracellular Potential
Transmembrane221 – 24121Helical; Potential
Topological domain242 – 26726Cytoplasmic Potential
Transmembrane268 – 28821Helical; Potential
Topological domain289 – 31628Extracellular Potential
Transmembrane317 – 33721Helical; Potential
Topological domain338 – 3414Cytoplasmic Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 37513Extracellular Potential
Transmembrane376 – 39621Helical; Potential
Topological domain397 – 4048Cytoplasmic Potential
Transmembrane405 – 42521Helical; Potential
Topological domain426 – 726301Extracellular Potential
Transmembrane727 – 74721Helical; Potential
Topological domain748 – 873126Cytoplasmic Potential

Amino acid modifications

Modified residue5571Phosphoserine Ref.6 Ref.7

Sequences

Sequence LengthMass (Da)Tools
P40309 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 881E55A1F59C8CE6

FASTA87397,111
        10         20         30         40         50         60 
MANTVGGILS GVNPFHYNSS SPLTLFLFQA CLILLVCNLI HIPFSMMRQP KVISEVISGV 

        70         80         90        100        110        120 
ILGPTIFGQI PNYTNTIFPT SSIPGLNLVA NLGIILFMFF LGLEVDIAFI KKHLKKALVI 

       130        140        150        160        170        180 
GIVTLAVPFG FGCLLAIPLF HTYANKTEGE RHIKFSVFMV FIAVSISVTA FPVLCRILNE 

       190        200        210        220        230        240 
LRLIKDRAGI VVLAAGIIND IMGWILLALS IILSSAEGSP VNTVYILLIT FAWFLIYFFP 

       250        260        270        280        290        300 
LKYLLRWVLI RTHELDRSKP SPLATMCILF IMFISAYFTD IIGVHPIFGA FIAGLVVPRD 

       310        320        330        340        350        360 
DHYVVKLTER MEDIPNIVFI PIYFAVAGLN VDLTLLNEGR DWGYVFATIG IAIFTKIISG 

       370        380        390        400        410        420 
TLTAKLTGLF WREATAAGVL MSCKGIVEIV VLTVGLNAGI ISRKIFGMFV LMALVSTFVT 

       430        440        450        460        470        480 
TPLTQLVYPD SYRDGVRKSL STPAEDDGAA DGLDSEGVDK TEINTQLNSL ADVSKYRIGE 

       490        500        510        520        530        540 
LTTVINTTEA ISPSLKLLNY LSLGVSPKPK NNKHKNETSL SRMTTATDST LKSNTFKIKK 

       550        560        570        580        590        600 
MVHIWSKSVD DVDTNLSVID EKLTPFEGVG ALRAIHLRLL TERTTDLLQS SSLYNDDPHF 

       610        620        630        640        650        660 
TANTDSLLQI FDIFSNLSKI PFSSEVIFST MREKAANIAT MKMDSTDLIL LPLKGASYEY 

       670        680        690        700        710        720 
RGSPVFIDEK YANFDHIYSH LLGLNELSST FFKSIFQSLK ANFAVQISNT YGRLNADRFK 

       730        740        750        760        770        780 
RKRFNLLLPK PYLTQSDYLG LYLLLLICYR DGYNNDNASC SIFINSKNID FAKDLSTAFA 

       790        800        810        820        830        840 
EHDWLNESTI KIVDIPFETK VPEEAIEKPS FIETVLDVGL SDTALADIEE TTFIIGEDLP 

       850        860        870 
DESEPFSEEV RTVIFEGSNR RFDTLIVHHF SSE 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X."
Miosga T., Witzel A., Zimmermann F.K.
Yeast 10:965-973(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77087 Genomic DNA. Translation: CAA54359.1.
Z49369 Genomic DNA. Translation: CAA89387.1.
BK006943 Genomic DNA. Translation: DAA08706.1.
PIRS46584.
RefSeqNP_012441.1. NM_001181527.1.

3D structure databases

ProteinModelPortalP40309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33663. 26 interactions.
DIPDIP-4799N.
IntActP40309. 1 interaction.
MINTMINT-494478.
STRING4932.YJL094C.

Protein family/group databases

TCDB2.A.37.4.1. the monovalent cation:proton antiporter-2 (cpa2) family.

Proteomic databases

PaxDbP40309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL094C; YJL094C; YJL094C.
GeneID853351.
KEGGsce:YJL094C.

Organism-specific databases

CYGDYJL094c.
SGDS000003630. KHA1.

Phylogenomic databases

eggNOGCOG0475.
HOGENOMHOG000247916.
OMATERMEDI.
OrthoDBEOG7GBG5B.

Enzyme and pathway databases

BioCycYEAST:G3O-31549-MONOMER.

Gene expression databases

GenevestigatorP40309.

Family and domain databases

InterProIPR006153. Cation/H_exchanger.
IPR004771. K/H_exchanger.
[Graphical view]
PfamPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
TIGRFAMsTIGR00932. 2a37. 1 hit.
ProtoNetSearch...

Other

NextBio973756.

Entry information

Entry nameKHA1_YEAST
AccessionPrimary (citable) accession number: P40309
Secondary accession number(s): D6VW90
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families