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P40309

- KHA1_YEAST

UniProt

P40309 - KHA1_YEAST

Protein

K(+)/H(+) antiporter 1

Gene

KHA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Potassium-proton antiport.

    GO - Molecular functioni

    1. potassium:proton antiporter activity Source: SGD

    GO - Biological processi

    1. monovalent inorganic cation transport Source: SGD
    2. potassium ion transmembrane transport Source: GOC

    Keywords - Biological processi

    Antiport, Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31549-MONOMER.

    Protein family/group databases

    TCDBi2.A.37.4.1. the monovalent cation:proton antiporter-2 (cpa2) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    K(+)/H(+) antiporter 1
    Gene namesi
    Name:KHA1
    Ordered Locus Names:YJL094C
    ORF Names:J0909
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL094c.
    SGDiS000003630. KHA1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 873873K(+)/H(+) antiporter 1PRO_0000196618Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei557 – 5571Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40309.
    PaxDbiP40309.

    Expressioni

    Gene expression databases

    GenevestigatoriP40309.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ADH1P003301EBI-25993,EBI-2218

    Protein-protein interaction databases

    BioGridi33663. 26 interactions.
    DIPiDIP-4799N.
    IntActiP40309. 1 interaction.
    MINTiMINT-494478.
    STRINGi4932.YJL094C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40309.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2323ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini45 – 517CytoplasmicSequence Analysis
    Topological domaini73 – 8210ExtracellularSequence Analysis
    Topological domaini104 – 11613CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini138 – 15417ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini176 – 18813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini210 – 22011ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini242 – 26726CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini289 – 31628ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini338 – 3414CytoplasmicSequence Analysis
    Topological domaini363 – 37513ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini397 – 4048CytoplasmicSequence Analysis
    Topological domaini426 – 726301ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini748 – 873126CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei52 – 7221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei83 – 10321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei155 – 17521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei221 – 24121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei268 – 28821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei317 – 33721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei376 – 39621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei405 – 42521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei727 – 74721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0475.
    HOGENOMiHOG000247916.
    OMAiTERMEDI.
    OrthoDBiEOG7GBG5B.

    Family and domain databases

    InterProiIPR006153. Cation/H_exchanger.
    IPR004771. K/H_exchanger.
    [Graphical view]
    PfamiPF00999. Na_H_Exchanger. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00932. 2a37. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40309-1 [UniParc]FASTAAdd to Basket

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    MANTVGGILS GVNPFHYNSS SPLTLFLFQA CLILLVCNLI HIPFSMMRQP    50
    KVISEVISGV ILGPTIFGQI PNYTNTIFPT SSIPGLNLVA NLGIILFMFF 100
    LGLEVDIAFI KKHLKKALVI GIVTLAVPFG FGCLLAIPLF HTYANKTEGE 150
    RHIKFSVFMV FIAVSISVTA FPVLCRILNE LRLIKDRAGI VVLAAGIIND 200
    IMGWILLALS IILSSAEGSP VNTVYILLIT FAWFLIYFFP LKYLLRWVLI 250
    RTHELDRSKP SPLATMCILF IMFISAYFTD IIGVHPIFGA FIAGLVVPRD 300
    DHYVVKLTER MEDIPNIVFI PIYFAVAGLN VDLTLLNEGR DWGYVFATIG 350
    IAIFTKIISG TLTAKLTGLF WREATAAGVL MSCKGIVEIV VLTVGLNAGI 400
    ISRKIFGMFV LMALVSTFVT TPLTQLVYPD SYRDGVRKSL STPAEDDGAA 450
    DGLDSEGVDK TEINTQLNSL ADVSKYRIGE LTTVINTTEA ISPSLKLLNY 500
    LSLGVSPKPK NNKHKNETSL SRMTTATDST LKSNTFKIKK MVHIWSKSVD 550
    DVDTNLSVID EKLTPFEGVG ALRAIHLRLL TERTTDLLQS SSLYNDDPHF 600
    TANTDSLLQI FDIFSNLSKI PFSSEVIFST MREKAANIAT MKMDSTDLIL 650
    LPLKGASYEY RGSPVFIDEK YANFDHIYSH LLGLNELSST FFKSIFQSLK 700
    ANFAVQISNT YGRLNADRFK RKRFNLLLPK PYLTQSDYLG LYLLLLICYR 750
    DGYNNDNASC SIFINSKNID FAKDLSTAFA EHDWLNESTI KIVDIPFETK 800
    VPEEAIEKPS FIETVLDVGL SDTALADIEE TTFIIGEDLP DESEPFSEEV 850
    RTVIFEGSNR RFDTLIVHHF SSE 873
    Length:873
    Mass (Da):97,111
    Last modified:February 1, 1995 - v1
    Checksum:i881E55A1F59C8CE6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77087 Genomic DNA. Translation: CAA54359.1.
    Z49369 Genomic DNA. Translation: CAA89387.1.
    BK006943 Genomic DNA. Translation: DAA08706.1.
    PIRiS46584.
    RefSeqiNP_012441.1. NM_001181527.1.

    Genome annotation databases

    EnsemblFungiiYJL094C; YJL094C; YJL094C.
    GeneIDi853351.
    KEGGisce:YJL094C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77087 Genomic DNA. Translation: CAA54359.1 .
    Z49369 Genomic DNA. Translation: CAA89387.1 .
    BK006943 Genomic DNA. Translation: DAA08706.1 .
    PIRi S46584.
    RefSeqi NP_012441.1. NM_001181527.1.

    3D structure databases

    ProteinModelPortali P40309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33663. 26 interactions.
    DIPi DIP-4799N.
    IntActi P40309. 1 interaction.
    MINTi MINT-494478.
    STRINGi 4932.YJL094C.

    Protein family/group databases

    TCDBi 2.A.37.4.1. the monovalent cation:proton antiporter-2 (cpa2) family.

    Proteomic databases

    MaxQBi P40309.
    PaxDbi P40309.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL094C ; YJL094C ; YJL094C .
    GeneIDi 853351.
    KEGGi sce:YJL094C.

    Organism-specific databases

    CYGDi YJL094c.
    SGDi S000003630. KHA1.

    Phylogenomic databases

    eggNOGi COG0475.
    HOGENOMi HOG000247916.
    OMAi TERMEDI.
    OrthoDBi EOG7GBG5B.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31549-MONOMER.

    Miscellaneous databases

    NextBioi 973756.

    Gene expression databases

    Genevestigatori P40309.

    Family and domain databases

    InterProi IPR006153. Cation/H_exchanger.
    IPR004771. K/H_exchanger.
    [Graphical view ]
    Pfami PF00999. Na_H_Exchanger. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00932. 2a37. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X."
      Miosga T., Witzel A., Zimmermann F.K.
      Yeast 10:965-973(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKHA1_YEAST
    AccessioniPrimary (citable) accession number: P40309
    Secondary accession number(s): D6VW90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 172 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3