K(+)/H(+) antiporter 1 - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P40309 (KHA1_YEAST)

Last modified June 16, 2009. Version 69. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
K(+)/H(+) antiporter 1

Gene names

Name:	KHA1
Ordered Locus Names:	YJL094C
ORF Names:	J0909

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Potassium-proton antiport.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 172 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. [View classification]

Hide | Top

Ontologies

Keywords
Biological process	Antiport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Potassium
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay. Source: SGD
Molecular function	potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium:hydrogen antiporter activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADH1	P00330	1	EBI-25993,EBI-2218

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 873

873

K(+)/H(+) antiporter 1

PRO_0000196618

Regions

Topological domain

1 – 23

Extracellular Potential

Transmembrane

24 – 44

Potential

Topological domain

45 – 51

Cytoplasmic Potential

Transmembrane

52 – 72

Potential

Topological domain

73 – 82

Extracellular Potential

Transmembrane

83 – 103

Potential

Topological domain

104 – 116

Cytoplasmic Potential

Transmembrane

117 – 137

Potential

Topological domain

138 – 154

Extracellular Potential

Transmembrane

155 – 175

Potential

Topological domain

176 – 188

Cytoplasmic Potential

Transmembrane

189 – 209

Potential

Topological domain

210 – 220

Extracellular Potential

Transmembrane

221 – 241

Potential

Topological domain

242 – 267

Cytoplasmic Potential

Transmembrane

268 – 288

Potential

Topological domain

289 – 316

Extracellular Potential

Transmembrane

317 – 337

Potential

Topological domain

338 – 341

Cytoplasmic Potential

Transmembrane

342 – 362

Potential

Topological domain

363 – 375

Extracellular Potential

Transmembrane

376 – 396

Potential

Topological domain

397 – 404

Cytoplasmic Potential

Transmembrane

405 – 425

Potential

Topological domain

426 – 726

301

Extracellular Potential

Transmembrane

727 – 747

Potential

Topological domain

748 – 873

126

Cytoplasmic Potential

Amino acid modifications

Modified residue

455

Phosphoserine Ref.6

Modified residue

557

Phosphoserine Ref.5

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P40309-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 881E55A1F59C8CE6
Show »

FASTA

873

97,111

        10         20         30         40         50         60 
MANTVGGILS GVNPFHYNSS SPLTLFLFQA CLILLVCNLI HIPFSMMRQP KVISEVISGV 

        70         80         90        100        110        120 
ILGPTIFGQI PNYTNTIFPT SSIPGLNLVA NLGIILFMFF LGLEVDIAFI KKHLKKALVI 

       130        140        150        160        170        180 
GIVTLAVPFG FGCLLAIPLF HTYANKTEGE RHIKFSVFMV FIAVSISVTA FPVLCRILNE 

       190        200        210        220        230        240 
LRLIKDRAGI VVLAAGIIND IMGWILLALS IILSSAEGSP VNTVYILLIT FAWFLIYFFP 

       250        260        270        280        290        300 
LKYLLRWVLI RTHELDRSKP SPLATMCILF IMFISAYFTD IIGVHPIFGA FIAGLVVPRD 

       310        320        330        340        350        360 
DHYVVKLTER MEDIPNIVFI PIYFAVAGLN VDLTLLNEGR DWGYVFATIG IAIFTKIISG 

       370        380        390        400        410        420 
TLTAKLTGLF WREATAAGVL MSCKGIVEIV VLTVGLNAGI ISRKIFGMFV LMALVSTFVT 

       430        440        450        460        470        480 
TPLTQLVYPD SYRDGVRKSL STPAEDDGAA DGLDSEGVDK TEINTQLNSL ADVSKYRIGE 

       490        500        510        520        530        540 
LTTVINTTEA ISPSLKLLNY LSLGVSPKPK NNKHKNETSL SRMTTATDST LKSNTFKIKK 

       550        560        570        580        590        600 
MVHIWSKSVD DVDTNLSVID EKLTPFEGVG ALRAIHLRLL TERTTDLLQS SSLYNDDPHF 

       610        620        630        640        650        660 
TANTDSLLQI FDIFSNLSKI PFSSEVIFST MREKAANIAT MKMDSTDLIL LPLKGASYEY 

       670        680        690        700        710        720 
RGSPVFIDEK YANFDHIYSH LLGLNELSST FFKSIFQSLK ANFAVQISNT YGRLNADRFK 

       730        740        750        760        770        780 
RKRFNLLLPK PYLTQSDYLG LYLLLLICYR DGYNNDNASC SIFINSKNID FAKDLSTAFA 

       790        800        810        820        830        840 
EHDWLNESTI KIVDIPFETK VPEEAIEKPS FIETVLDVGL SDTALADIEE TTFIIGEDLP 

       850        860        870 
DESEPFSEEV RTVIFEGSNR RFDTLIVHHF SSE

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X." Miosga T., Witzel A., Zimmermann F.K. Yeast 10:965-973(1994) [PubMed: 7985424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-557, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	X77087 Genomic DNA. Translation: CAA54359.1. Z49369 Genomic DNA. Translation: CAA89387.1.
PIR	S46584.
RefSeq	NP_012441.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4799N.
IntAct	P40309. 2 interactions.
Protein family/group databases
TCDB	2.A.37.4.1. monovalent cation:proton antiporter-2 (CPA2) family.
Genome annotation databases
Ensembl	YJL094C. Saccharomyces cerevisiae. [Contig view]
GeneID	853351.
GenomeReviews	Gene locus YJL094C in contig Y13136_GR.
KEGG	sce:YJL094C.
NMPDR	fig\|4932.3.peg.3410.
Organism-specific databases
CYGD	YJL094c.
SGD	S000003630. KHA1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P40309.
OMA	P40309. TERMEDI.
Gene expression databases
ArrayExpress	P40309.
GermOnline	YJL094C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006153. Cation/H_exchanger. IPR004771. K/H_exchanger. [Graphical view]
Pfam	PF00999. Na_H_Exchanger. 1 hit. [Graphical view]
TIGRFAMs	TIGR00932. 2a37. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	973756.

Hide | Top

Entry information

Entry name

KHA1_YEAST

Accession

Primary (citable) accession number: P40309

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents