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Protein

Lipase 3

Gene

TGL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases specific fatty acids from neutral lipid triacylglycerols (TAG) thereby supplying fatty acids to a general acylation process.2 Publications

Miscellaneous

Present with 3210 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei237By similarity1

GO - Molecular functioni

  • lysophosphatidylethanolamine acyltransferase activity Source: SGD
  • triglyceride lipase activity Source: SGD

GO - Biological processi

  • cell budding Source: SGD
  • cellular lipid metabolic process Source: SGD
  • triglyceride catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32977-MONOMER
YEAST:G3O-32977-MONOMER

Chemistry databases

SwissLipidsiSLP:000000052
SLP:000000671
SLP:000000678

Names & Taxonomyi

Protein namesi
Recommended name:
Lipase 3 (EC:3.1.1.3)
Alternative name(s):
Triacylglycerol lipase 3
Gene namesi
Name:TGL3
Ordered Locus Names:YMR313C
ORF Names:YM9924.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR313C
SGDiS000004930 TGL3

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002033561 – 642Lipase 3Add BLAST642

Proteomic databases

MaxQBiP40308
PaxDbiP40308
PRIDEiP40308

PTM databases

iPTMnetiP40308

Interactioni

Protein-protein interaction databases

BioGridi35493, 85 interactors
DIPiDIP-5641N
IntActiP40308, 5 interactors
MINTiP40308
STRINGi4932.YMR313C

Structurei

3D structure databases

ProteinModelPortaliP40308
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini204 – 392PNPLAPROSITE-ProRule annotationAdd BLAST189

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi235 – 239GXSXGPROSITE-ProRule annotation5

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016747
HOGENOMiHOG000208720
InParanoidiP40308
KOiK14675
OMAiGERSVWP
OrthoDBiEOG092C1ELU

Family and domain databases

InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR002641 PNPLA_dom
IPR021771 Triacylglycerol_lipase
PfamiView protein in Pfam
PF11815 DUF3336, 1 hit
PF01734 Patatin, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS51635 PNPLA, 1 hit

Sequencei

Sequence statusi: Complete.

P40308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKETAQEYKV SAVIPTLLKN WILRVVYATL DHIPPFVWEI LHVITDIYFF
60 70 80 90 100
WVQKLINYVR PHSRVIYYNA IKKLDECDTY QMWCQQASVV DEITGANLWR
110 120 130 140 150
RNFFSRRYDF NSVIEQYSIL ENMLREEKYD VVKEKFSTTG PCMLRNFAGI
160 170 180 190 200
GDKKLFTKSL MGTKLLIEQY LTRILEGLDI LNNQTLTPTS FFQRCKLSLG
210 220 230 240 250
TTALILQGGS LFGLFHLGVI RGLLLQDLMP NIISGSSMGA CVASLFGCLS
260 270 280 290 300
NEQLKQLLTD DNLLNIIKND VDLLKSCGYG NLEQHLNLGT LIQNLIHHGY
310 320 330 340 350
SQDVYLFIRF VMKYIVKEKT FEEVYQITGK VFNIVIHPTD KSCPNLLNYV
360 370 380 390 400
TTPNVLIKSA IECSLGSGVI SEDTSLLCKN LENEIEPFLN INKNKQVKFL
410 420 430 440 450
TPENANNPSI TESPYTRLTE LFNVNNFIVS LARPYLAPLV VNDLKHEIKT
460 470 480 490 500
SKYYYYKHYP NMPPINANTV RKTQRSSSQS PIKAGTVEDL EPEPLMSPVP
510 520 530 540 550
PSSAVNDSAE YIIPELGIPQ LNFTEMEPLA FKFKYHLERK LKNIATMEFR
560 570 580 590 600
HRMEVLDNLG LLCSLIKRLI IDEKTPRSAT EIAVVPRMKS LSLTRIIEGQ
610 620 630 640
LNNIPYWIKS GERSTWPALA LIKTRCAVEF KLDDIIRARR SR
Length:642
Mass (Da):73,613
Last modified:October 1, 1996 - v2
Checksum:iA5AE059EF65465D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96A → P in AAA53543 (PubMed:7918444).Curated1
Sequence conflicti100R → P in AAA53543 (PubMed:7918444).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA Translation: CAA90831.1
L34347 Genomic DNA Translation: AAA53543.1
BK006946 Genomic DNA Translation: DAA10214.1
PIRiS59306
RefSeqiNP_014044.1, NM_001182824.1

Genome annotation databases

EnsemblFungiiYMR313C; YMR313C; YMR313C
GeneIDi855361
KEGGisce:YMR313C

Similar proteinsi

Entry informationi

Entry nameiTGL3_YEAST
AccessioniPrimary (citable) accession number: P40308
Secondary accession number(s): D6W0E0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health