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P40307

- PSB2_RAT

UniProt

P40307 - PSB2_RAT

Protein

Proteasome subunit beta type-2

Gene

Psmb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a chymotrypsin-like activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis involved in cellular protein catabolic process Source: InterPro
    2. response to organic cyclic compound Source: RGD
    3. response to organonitrogen compound Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_198391. Asymmetric localization of PCP proteins.
    REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199197. ER-Phagosome pathway.
    REACT_199247. Activation of NF-kappaB in B cells.
    REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206488. degradation of DVL.
    REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
    REACT_211117. Orc1 removal from chromatin.
    REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_220232. Regulation of ornithine decarboxylase (ODC).
    REACT_227706. degradation of AXIN.

    Protein family/group databases

    MEROPSiT01.984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-2 (EC:3.4.25.1)
    Alternative name(s):
    Macropain subunit C7-I
    Multicatalytic endopeptidase complex subunit C7-I
    Proteasome component C7-I
    Gene namesi
    Name:Psmb2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi61874. Psmb2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 201201Proteasome subunit beta type-2PRO_0000148045Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP40307.
    PRIDEiP40307.

    Expressioni

    Inductioni

    Down-regulated by theophylline (THP) and 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.1 Publication

    Gene expression databases

    GenevestigatoriP40307.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

    Protein-protein interaction databases

    IntActiP40307. 1 interaction.
    STRINGi10116.ENSRNOP00000015618.

    Structurei

    3D structure databases

    ProteinModelPortaliP40307.
    SMRiP40307. Positions 1-199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00640000091536.
    HOGENOMiHOG000188743.
    HOVERGENiHBG000815.
    InParanoidiP40307.
    KOiK02734.
    OMAiHFVRGEL.
    OrthoDBiEOG7N63NQ.
    PhylomeDBiP40307.
    TreeFamiTF106219.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40307-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA    50
    GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV 100
    NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT 150
    ISRERAVELL RKCLEELQKR FILNLPTFSV RVIDKDGIHN LENITFTKRS 200
    S 201
    Length:201
    Mass (Da):22,912
    Last modified:February 1, 1995 - v1
    Checksum:iE9AC021972B76528
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701R → L AA sequence (PubMed:8405448)Curated
    Sequence conflicti81 – 811A → V AA sequence (PubMed:8405448)Curated
    Sequence conflicti91 – 911C → P AA sequence (PubMed:8405448)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21799 mRNA. Translation: BAA04823.1.
    BC058487 mRNA. Translation: AAH58487.1.
    PIRiS38725.
    RefSeqiNP_058980.1. NM_017284.1.
    UniGeneiRn.1981.

    Genome annotation databases

    EnsembliENSRNOT00000015618; ENSRNOP00000015618; ENSRNOG00000011463.
    GeneIDi29675.
    KEGGirno:29675.
    UCSCiRGD:61874. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21799 mRNA. Translation: BAA04823.1 .
    BC058487 mRNA. Translation: AAH58487.1 .
    PIRi S38725.
    RefSeqi NP_058980.1. NM_017284.1.
    UniGenei Rn.1981.

    3D structure databases

    ProteinModelPortali P40307.
    SMRi P40307. Positions 1-199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P40307. 1 interaction.
    STRINGi 10116.ENSRNOP00000015618.

    Protein family/group databases

    MEROPSi T01.984.

    Proteomic databases

    PaxDbi P40307.
    PRIDEi P40307.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015618 ; ENSRNOP00000015618 ; ENSRNOG00000011463 .
    GeneIDi 29675.
    KEGGi rno:29675.
    UCSCi RGD:61874. rat.

    Organism-specific databases

    CTDi 5690.
    RGDi 61874. Psmb2.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00640000091536.
    HOGENOMi HOG000188743.
    HOVERGENi HBG000815.
    InParanoidi P40307.
    KOi K02734.
    OMAi HFVRGEL.
    OrthoDBi EOG7N63NQ.
    PhylomeDBi P40307.
    TreeFami TF106219.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_198391. Asymmetric localization of PCP proteins.
    REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199197. ER-Phagosome pathway.
    REACT_199247. Activation of NF-kappaB in B cells.
    REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206488. degradation of DVL.
    REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
    REACT_211117. Orc1 removal from chromatin.
    REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_220232. Regulation of ornithine decarboxylase (ODC).
    REACT_227706. degradation of AXIN.

    Miscellaneous databases

    NextBioi 610011.
    PROi P40307.

    Gene expression databases

    Genevestigatori P40307.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of rat proteasome subunit RC7-I, a homologue of yeast PRE1 essential for chymotrypsin-like activity."
      Nishimura C., Tamura T., Tokunaga F., Tanaka K., Ichihara A.
      FEBS Lett. 332:52-56(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    3. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 171-181, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.
    4. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
      Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
      Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY THP AND DNB.

    Entry informationi

    Entry nameiPSB2_RAT
    AccessioniPrimary (citable) accession number: P40307
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3