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Protein

Proteasome subunit beta type-2

Gene

Psmb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a chymotrypsin-like activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_272498. APC/C:Cdc20 mediated degradation of Securin.
REACT_274511. Regulation of ornithine decarboxylase (ODC).
REACT_275019. Ubiquitin-dependent degradation of Cyclin D1.
REACT_277636. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_280071. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_280715. GLI3 is processed to GLI3R by the proteasome.
REACT_287195. Degradation of GLI2 by the proteasome.
REACT_288207. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_289020. Degradation of beta-catenin by the destruction complex.
REACT_290448. Orc1 removal from chromatin.
REACT_295138. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_297906. Separation of Sister Chromatids.
REACT_305105. degradation of DVL.
REACT_309656. SCF-beta-TrCP mediated degradation of Emi1.
REACT_312094. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_317312. SCF(Skp2)-mediated degradation of p27/p21.
REACT_318052. Activation of NF-kappaB in B cells.
REACT_325005. CDT1 association with the CDC6:ORC:origin complex.
REACT_327126. Hedgehog 'on' state.
REACT_333644. Asymmetric localization of PCP proteins.
REACT_334373. degradation of AXIN.
REACT_336450. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_336611. Hedgehog ligand biogenesis.
REACT_338477. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_343414. ER-Phagosome pathway.
REACT_346077. CDK-mediated phosphorylation and removal of Cdc6.
REACT_349901. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_357442. Degradation of GLI1 by the proteasome.
REACT_359556. CLEC7A (Dectin-1) signaling.
REACT_361793. Dectin-1 mediated noncanonical NF-kB signaling.

Protein family/group databases

MEROPSiT01.984.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-2 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C7-I
Multicatalytic endopeptidase complex subunit C7-I
Proteasome component C7-I
Gene namesi
Name:Psmb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61874. Psmb2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Proteasome subunit beta type-2PRO_0000148045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP40307.
PRIDEiP40307.

Expressioni

Inductioni

Down-regulated by theophylline (THP) and 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.1 Publication

Gene expression databases

GenevisibleiP40307. RN.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Protein-protein interaction databases

IntActiP40307. 1 interaction.
STRINGi10116.ENSRNOP00000015618.

Structurei

3D structure databases

ProteinModelPortaliP40307.
SMRiP40307. Positions 1-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00640000091536.
HOGENOMiHOG000188743.
HOVERGENiHBG000815.
InParanoidiP40307.
KOiK02734.
OMAiHFVRGEL.
OrthoDBiEOG7N63NQ.
PhylomeDBiP40307.
TreeFamiTF106219.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA
60 70 80 90 100
GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV
110 120 130 140 150
NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT
160 170 180 190 200
ISRERAVELL RKCLEELQKR FILNLPTFSV RVIDKDGIHN LENITFTKRS

S
Length:201
Mass (Da):22,912
Last modified:February 1, 1995 - v1
Checksum:iE9AC021972B76528
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701R → L AA sequence (PubMed:8405448).Curated
Sequence conflicti81 – 811A → V AA sequence (PubMed:8405448).Curated
Sequence conflicti91 – 911C → P AA sequence (PubMed:8405448).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21799 mRNA. Translation: BAA04823.1.
BC058487 mRNA. Translation: AAH58487.1.
PIRiS38725.
RefSeqiNP_058980.1. NM_017284.1.
UniGeneiRn.1981.

Genome annotation databases

EnsembliENSRNOT00000015618; ENSRNOP00000015618; ENSRNOG00000011463.
GeneIDi29675.
KEGGirno:29675.
UCSCiRGD:61874. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21799 mRNA. Translation: BAA04823.1.
BC058487 mRNA. Translation: AAH58487.1.
PIRiS38725.
RefSeqiNP_058980.1. NM_017284.1.
UniGeneiRn.1981.

3D structure databases

ProteinModelPortaliP40307.
SMRiP40307. Positions 1-199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP40307. 1 interaction.
STRINGi10116.ENSRNOP00000015618.

Protein family/group databases

MEROPSiT01.984.

Proteomic databases

PaxDbiP40307.
PRIDEiP40307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015618; ENSRNOP00000015618; ENSRNOG00000011463.
GeneIDi29675.
KEGGirno:29675.
UCSCiRGD:61874. rat.

Organism-specific databases

CTDi5690.
RGDi61874. Psmb2.

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00640000091536.
HOGENOMiHOG000188743.
HOVERGENiHBG000815.
InParanoidiP40307.
KOiK02734.
OMAiHFVRGEL.
OrthoDBiEOG7N63NQ.
PhylomeDBiP40307.
TreeFamiTF106219.

Enzyme and pathway databases

ReactomeiREACT_272498. APC/C:Cdc20 mediated degradation of Securin.
REACT_274511. Regulation of ornithine decarboxylase (ODC).
REACT_275019. Ubiquitin-dependent degradation of Cyclin D1.
REACT_277636. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_280071. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_280715. GLI3 is processed to GLI3R by the proteasome.
REACT_287195. Degradation of GLI2 by the proteasome.
REACT_288207. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_289020. Degradation of beta-catenin by the destruction complex.
REACT_290448. Orc1 removal from chromatin.
REACT_295138. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_297906. Separation of Sister Chromatids.
REACT_305105. degradation of DVL.
REACT_309656. SCF-beta-TrCP mediated degradation of Emi1.
REACT_312094. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_317312. SCF(Skp2)-mediated degradation of p27/p21.
REACT_318052. Activation of NF-kappaB in B cells.
REACT_325005. CDT1 association with the CDC6:ORC:origin complex.
REACT_327126. Hedgehog 'on' state.
REACT_333644. Asymmetric localization of PCP proteins.
REACT_334373. degradation of AXIN.
REACT_336450. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_336611. Hedgehog ligand biogenesis.
REACT_338477. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_343414. ER-Phagosome pathway.
REACT_346077. CDK-mediated phosphorylation and removal of Cdc6.
REACT_349901. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_357442. Degradation of GLI1 by the proteasome.
REACT_359556. CLEC7A (Dectin-1) signaling.
REACT_361793. Dectin-1 mediated noncanonical NF-kB signaling.

Miscellaneous databases

NextBioi610011.
PROiP40307.

Gene expression databases

GenevisibleiP40307. RN.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of rat proteasome subunit RC7-I, a homologue of yeast PRE1 essential for chymotrypsin-like activity."
    Nishimura C., Tamura T., Tokunaga F., Tanaka K., Ichihara A.
    FEBS Lett. 332:52-56(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 171-181, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  4. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP AND DNB.

Entry informationi

Entry nameiPSB2_RAT
AccessioniPrimary (citable) accession number: P40307
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.