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P40261

- NNMT_HUMAN

UniProt

P40261 - NNMT_HUMAN

Protein

Nicotinamide N-methyltransferase

Gene

NNMT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

    Catalytic activityi

    S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201S-adenosyl-L-methionine
    Binding sitei25 – 251S-adenosyl-L-methionine
    Binding sitei69 – 691S-adenosyl-L-methionine
    Binding sitei85 – 851S-adenosyl-L-methionine
    Binding sitei90 – 901S-adenosyl-L-methionine
    Binding sitei163 – 1631S-adenosyl-L-methionine

    GO - Molecular functioni

    1. nicotinamide N-methyltransferase activity Source: ProtInc

    GO - Biological processi

    1. methylation Source: Reactome
    2. organ regeneration Source: Ensembl
    3. response to drug Source: Ensembl
    4. response to organonitrogen compound Source: Ensembl
    5. small molecule metabolic process Source: Reactome
    6. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    ReactomeiREACT_6946. Methylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide N-methyltransferase (EC:2.1.1.1)
    Gene namesi
    Name:NNMT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:7861. NNMT.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA251.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Nicotinamide N-methyltransferasePRO_0000159706Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei39 – 391N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP40261.
    PaxDbiP40261.
    PeptideAtlasiP40261.
    PRIDEiP40261.

    PTM databases

    PhosphoSiteiP40261.

    Expressioni

    Tissue specificityi

    Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

    Gene expression databases

    ArrayExpressiP40261.
    BgeeiP40261.
    CleanExiHS_NNMT.
    GenevestigatoriP40261.

    Organism-specific databases

    HPAiHPA059180.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi110900. 5 interactions.
    IntActiP40261. 2 interactions.
    STRINGi9606.ENSP00000299964.

    Structurei

    Secondary structure

    1
    264
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 147
    Helixi17 – 259
    Helixi33 – 4917
    Beta strandi56 – 627
    Helixi69 – 713
    Helixi74 – 763
    Beta strandi78 – 869
    Helixi88 – 9811
    Helixi108 – 11710
    Helixi124 – 1329
    Beta strandi135 – 1406
    Beta strandi145 – 1473
    Turni148 – 1514
    Beta strandi157 – 1648
    Helixi166 – 1694
    Helixi173 – 18412
    Beta strandi187 – 20014
    Beta strandi203 – 2064
    Beta strandi209 – 2124
    Helixi218 – 2269
    Turni227 – 2293
    Beta strandi230 – 2389
    Turni244 – 2463
    Beta strandi252 – 2598

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IIPX-ray2.05A/B/C/D1-264[»]
    3RODX-ray2.72A/B/C/D1-264[»]
    ProteinModelPortaliP40261.
    SMRiP40261. Positions 3-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40261.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 642S-adenosyl-L-methionine binding
    Regioni142 – 1432S-adenosyl-L-methionine binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG71857.
    HOGENOMiHOG000013229.
    HOVERGENiHBG000797.
    InParanoidiP40261.
    KOiK00541.
    OMAiLKSSYYM.
    OrthoDBiEOG7673B9.
    PhylomeDBiP40261.
    TreeFamiTF313114.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025818. NNMT.
    IPR025820. NNMT/PNMT/TEMT_CS.
    IPR000940. NNMT_TEMT_trans.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10867. PTHR10867. 1 hit.
    PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000384. PNMTase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
    PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40261-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC    50
    LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK 100
    EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG 150
    AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK 200
    SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE 250
    GLFSLVARKL SRPL 264
    Length:264
    Mass (Da):29,574
    Last modified:February 1, 1995 - v1
    Checksum:i280B12748F4488AC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08021 mRNA. Translation: AAA19904.1.
    U20971, U20970 Genomic DNA. Translation: AAA93158.1.
    BC000234 mRNA. Translation: AAH00234.1.
    CCDSiCCDS8368.1.
    PIRiA54060.
    RefSeqiNP_006160.1. NM_006169.2.
    UniGeneiHs.503911.

    Genome annotation databases

    GeneIDi4837.
    KEGGihsa:4837.
    UCSCiuc001por.1. human.

    Polymorphism databases

    DMDMi730163.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08021 mRNA. Translation: AAA19904.1 .
    U20971 , U20970 Genomic DNA. Translation: AAA93158.1 .
    BC000234 mRNA. Translation: AAH00234.1 .
    CCDSi CCDS8368.1.
    PIRi A54060.
    RefSeqi NP_006160.1. NM_006169.2.
    UniGenei Hs.503911.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IIP X-ray 2.05 A/B/C/D 1-264 [» ]
    3ROD X-ray 2.72 A/B/C/D 1-264 [» ]
    ProteinModelPortali P40261.
    SMRi P40261. Positions 3-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110900. 5 interactions.
    IntActi P40261. 2 interactions.
    STRINGi 9606.ENSP00000299964.

    Chemistry

    ChEMBLi CHEMBL2346486.
    DrugBanki DB00627. Niacin.

    PTM databases

    PhosphoSitei P40261.

    Polymorphism databases

    DMDMi 730163.

    Proteomic databases

    MaxQBi P40261.
    PaxDbi P40261.
    PeptideAtlasi P40261.
    PRIDEi P40261.

    Protocols and materials databases

    DNASUi 4837.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4837.
    KEGGi hsa:4837.
    UCSCi uc001por.1. human.

    Organism-specific databases

    CTDi 4837.
    GeneCardsi GC11P114162.
    HGNCi HGNC:7861. NNMT.
    HPAi HPA059180.
    MIMi 600008. gene.
    neXtProti NX_P40261.
    PharmGKBi PA251.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG71857.
    HOGENOMi HOG000013229.
    HOVERGENi HBG000797.
    InParanoidi P40261.
    KOi K00541.
    OMAi LKSSYYM.
    OrthoDBi EOG7673B9.
    PhylomeDBi P40261.
    TreeFami TF313114.

    Enzyme and pathway databases

    Reactomei REACT_6946. Methylation.

    Miscellaneous databases

    EvolutionaryTracei P40261.
    GeneWikii NNMT.
    GenomeRNAii 4837.
    NextBioi 18638.
    PROi P40261.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P40261.
    Bgeei P40261.
    CleanExi HS_NNMT.
    Genevestigatori P40261.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025818. NNMT.
    IPR025820. NNMT/PNMT/TEMT_CS.
    IPR000940. NNMT_TEMT_trans.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10867. PTHR10867. 1 hit.
    Pfami PF01234. NNMT_PNMT_TEMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000384. PNMTase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS01100. NNMT_PNMT_TEMT. 1 hit.
    PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human liver nicotinamide N-methyltransferase. cDNA cloning, expression, and biochemical characterization."
      Aksoy S., Szumlanski C.L., Weinshilboum R.M.
      J. Biol. Chem. 269:14835-14840(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-16 AND 151-184.
      Tissue: Liver.
    2. "Human nicotinamide N-methyltransferase gene: molecular cloning, structural characterization and chromosomal localization."
      Aksoy S., Brandriff B.F., Ward A., Little P.F., Weinshilboum R.M.
      Genomics 29:555-561(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. "Human ERp29: isolation, primary structural characterisation and two-dimensional gel mapping."
      Hubbard M.J., McHugh N.J.
      Electrophoresis 21:3785-3796(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Liver.
    5. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "The crystal structure of human nicotinamide N-methyltransferase in complex with SAH."
      Structural genomics consortium (SGC)
      Submitted (OCT-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.

    Entry informationi

    Entry nameiNNMT_HUMAN
    AccessioniPrimary (citable) accession number: P40261
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3