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Protein

Nicotinamide N-methyltransferase

Gene

NNMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityi

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methionine1 Publication1
Binding sitei25S-adenosyl-L-methionine1 Publication1
Binding sitei69S-adenosyl-L-methionine1 Publication1
Binding sitei85S-adenosyl-L-methionine1 Publication1
Binding sitei90S-adenosyl-L-methionine1 Publication1
Binding sitei163S-adenosyl-L-methionine1 Publication1

GO - Molecular functioni

  • nicotinamide N-methyltransferase activity Source: ProtInc
  • pyridine N-methyltransferase activity Source: Reactome

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZFISH:HS09444-MONOMER.
BRENDAi2.1.1.1. 2681.
ReactomeiR-HSA-156581. Methylation.
R-HSA-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesi
Name:NNMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:7861. NNMT.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi4837.
OpenTargetsiENSG00000166741.
PharmGKBiPA251.

Chemistry databases

ChEMBLiCHEMBL2346486.
DrugBankiDB00627. Niacin.

Polymorphism and mutation databases

BioMutaiNNMT.
DMDMi730163.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597061 – 264Nicotinamide N-methyltransferaseAdd BLAST264

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP40261.
PaxDbiP40261.
PeptideAtlasiP40261.
PRIDEiP40261.
TopDownProteomicsiP40261.

PTM databases

iPTMnetiP40261.
PhosphoSitePlusiP40261.

Expressioni

Tissue specificityi

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Gene expression databases

BgeeiENSG00000166741.
CleanExiHS_NNMT.
ExpressionAtlasiP40261. baseline and differential.
GenevisibleiP40261. HS.

Organism-specific databases

HPAiHPA059180.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi110900. 6 interactors.
IntActiP40261. 2 interactors.
STRINGi9606.ENSP00000299964.

Chemistry databases

BindingDBiP40261.

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni1 – 3Combined sources3
Helixi10 – 13Combined sources4
Helixi17 – 24Combined sources8
Helixi33 – 50Combined sources18
Beta strandi56 – 63Combined sources8
Helixi69 – 71Combined sources3
Helixi74 – 77Combined sources4
Beta strandi78 – 86Combined sources9
Helixi88 – 98Combined sources11
Helixi108 – 117Combined sources10
Beta strandi120 – 122Combined sources3
Helixi124 – 134Combined sources11
Beta strandi135 – 140Combined sources6
Beta strandi145 – 147Combined sources3
Turni148 – 151Combined sources4
Beta strandi157 – 164Combined sources8
Helixi166 – 169Combined sources4
Helixi173 – 184Combined sources12
Beta strandi187 – 200Combined sources14
Beta strandi203 – 206Combined sources4
Beta strandi209 – 212Combined sources4
Helixi218 – 227Combined sources10
Beta strandi230 – 238Combined sources9
Turni244 – 246Combined sources3
Beta strandi252 – 259Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IIPX-ray2.05A/B/C/D1-264[»]
3RODX-ray2.72A/B/C/D1-264[»]
ProteinModelPortaliP40261.
SMRiP40261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40261.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 64S-adenosyl-L-methionine binding1 Publication2
Regioni142 – 143S-adenosyl-L-methionine binding1 Publication2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFTZ. Eukaryota.
ENOG41128ZR. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiP40261.
KOiK00541.
OMAiEWFEVIS.
OrthoDBiEOG091G0EF7.
PhylomeDBiP40261.
TreeFamiTF313114.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC
60 70 80 90 100
LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK
110 120 130 140 150
EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG
160 170 180 190 200
AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE
260
GLFSLVARKL SRPL
Length:264
Mass (Da):29,574
Last modified:February 1, 1995 - v1
Checksum:i280B12748F4488AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08021 mRNA. Translation: AAA19904.1.
U20971, U20970 Genomic DNA. Translation: AAA93158.1.
BC000234 mRNA. Translation: AAH00234.1.
CCDSiCCDS8368.1.
PIRiA54060.
RefSeqiNP_006160.1. NM_006169.2.
UniGeneiHs.503911.

Genome annotation databases

EnsembliENST00000299964; ENSP00000299964; ENSG00000166741.
ENST00000535401; ENSP00000441434; ENSG00000166741.
GeneIDi4837.
KEGGihsa:4837.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08021 mRNA. Translation: AAA19904.1.
U20971, U20970 Genomic DNA. Translation: AAA93158.1.
BC000234 mRNA. Translation: AAH00234.1.
CCDSiCCDS8368.1.
PIRiA54060.
RefSeqiNP_006160.1. NM_006169.2.
UniGeneiHs.503911.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IIPX-ray2.05A/B/C/D1-264[»]
3RODX-ray2.72A/B/C/D1-264[»]
ProteinModelPortaliP40261.
SMRiP40261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110900. 6 interactors.
IntActiP40261. 2 interactors.
STRINGi9606.ENSP00000299964.

Chemistry databases

BindingDBiP40261.
ChEMBLiCHEMBL2346486.
DrugBankiDB00627. Niacin.

PTM databases

iPTMnetiP40261.
PhosphoSitePlusiP40261.

Polymorphism and mutation databases

BioMutaiNNMT.
DMDMi730163.

Proteomic databases

EPDiP40261.
PaxDbiP40261.
PeptideAtlasiP40261.
PRIDEiP40261.
TopDownProteomicsiP40261.

Protocols and materials databases

DNASUi4837.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299964; ENSP00000299964; ENSG00000166741.
ENST00000535401; ENSP00000441434; ENSG00000166741.
GeneIDi4837.
KEGGihsa:4837.

Organism-specific databases

CTDi4837.
DisGeNETi4837.
GeneCardsiNNMT.
HGNCiHGNC:7861. NNMT.
HPAiHPA059180.
MIMi600008. gene.
neXtProtiNX_P40261.
OpenTargetsiENSG00000166741.
PharmGKBiPA251.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFTZ. Eukaryota.
ENOG41128ZR. LUCA.
GeneTreeiENSGT00390000011708.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiP40261.
KOiK00541.
OMAiEWFEVIS.
OrthoDBiEOG091G0EF7.
PhylomeDBiP40261.
TreeFamiTF313114.

Enzyme and pathway databases

BioCyciZFISH:HS09444-MONOMER.
BRENDAi2.1.1.1. 2681.
ReactomeiR-HSA-156581. Methylation.
R-HSA-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

ChiTaRSiNNMT. human.
EvolutionaryTraceiP40261.
GeneWikiiNNMT.
GenomeRNAii4837.
PROiP40261.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166741.
CleanExiHS_NNMT.
ExpressionAtlasiP40261. baseline and differential.
GenevisibleiP40261. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNMT_HUMAN
AccessioniPrimary (citable) accession number: P40261
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.