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Protein

Nicotinamide N-methyltransferase

Gene

NNMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Catalytic activityi

S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methionine1 Publication1
Binding sitei25S-adenosyl-L-methionine1 Publication1
Binding sitei69S-adenosyl-L-methionine1 Publication1
Binding sitei85S-adenosyl-L-methionine1 Publication1
Binding sitei90S-adenosyl-L-methionine1 Publication1
Binding sitei163S-adenosyl-L-methionine1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.1 2681
ReactomeiR-HSA-156581 Methylation
R-HSA-197264 Nicotinamide salvaging
R-HSA-2408508 Metabolism of ingested SeMet, Sec, MeSec into H2Se

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide N-methyltransferase (EC:2.1.1.1)
Gene namesi
Name:NNMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000166741.7
HGNCiHGNC:7861 NNMT
MIMi600008 gene
neXtProtiNX_P40261

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi4837
OpenTargetsiENSG00000166741
PharmGKBiPA251

Chemistry databases

ChEMBLiCHEMBL2346486
DrugBankiDB00627 Niacin

Polymorphism and mutation databases

BioMutaiNNMT
DMDMi730163

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001597061 – 264Nicotinamide N-methyltransferaseAdd BLAST264

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP40261
PaxDbiP40261
PeptideAtlasiP40261
PRIDEiP40261
TopDownProteomicsiP40261

PTM databases

iPTMnetiP40261
PhosphoSitePlusiP40261

Expressioni

Tissue specificityi

Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.

Gene expression databases

BgeeiENSG00000166741
CleanExiHS_NNMT
ExpressionAtlasiP40261 baseline and differential
GenevisibleiP40261 HS

Organism-specific databases

HPAiHPA059180

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi110900, 7 interactors
IntActiP40261, 2 interactors
STRINGi9606.ENSP00000299964

Chemistry databases

BindingDBiP40261

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni1 – 3Combined sources3
Helixi10 – 13Combined sources4
Helixi17 – 24Combined sources8
Helixi33 – 50Combined sources18
Beta strandi56 – 63Combined sources8
Helixi69 – 71Combined sources3
Helixi74 – 77Combined sources4
Beta strandi78 – 86Combined sources9
Helixi88 – 98Combined sources11
Helixi108 – 117Combined sources10
Beta strandi120 – 122Combined sources3
Helixi124 – 134Combined sources11
Beta strandi135 – 140Combined sources6
Beta strandi145 – 147Combined sources3
Turni148 – 151Combined sources4
Beta strandi157 – 164Combined sources8
Helixi166 – 169Combined sources4
Helixi173 – 184Combined sources12
Beta strandi187 – 200Combined sources14
Beta strandi203 – 206Combined sources4
Beta strandi209 – 212Combined sources4
Helixi218 – 227Combined sources10
Beta strandi230 – 238Combined sources9
Turni244 – 246Combined sources3
Beta strandi252 – 259Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IIPX-ray2.05A/B/C/D1-264[»]
3RODX-ray2.72A/B/C/D1-264[»]
5YJFX-ray2.49A/B/C/D1-264[»]
6B1AX-ray2.30A/B/C/D1-264[»]
ProteinModelPortaliP40261
SMRiP40261
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40261

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 64S-adenosyl-L-methionine binding1 Publication2
Regioni142 – 143S-adenosyl-L-methionine binding1 Publication2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFTZ Eukaryota
ENOG41128ZR LUCA
GeneTreeiENSGT00390000011708
HOGENOMiHOG000013229
HOVERGENiHBG000797
InParanoidiP40261
KOiK00541
OMAiEWFEVIS
OrthoDBiEOG091G0EF7
PhylomeDBiP40261
TreeFamiTF313114

Family and domain databases

InterProiView protein in InterPro
IPR025820 NNMT/PNMT/TEMT_CS
IPR000940 NNMT_TEMT_trans
IPR029063 SAM-dependent_MTases
PANTHERiPTHR10867 PTHR10867, 1 hit
PfamiView protein in Pfam
PF01234 NNMT_PNMT_TEMT, 1 hit
PIRSFiPIRSF000384 PNMTase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS01100 NNMT_PNMT_TEMT, 1 hit
PS51681 SAM_MT_NNMT_PNMT_TEMT, 1 hit

Sequencei

Sequence statusi: Complete.

P40261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC
60 70 80 90 100
LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK
110 120 130 140 150
EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG
160 170 180 190 200
AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE
260
GLFSLVARKL SRPL
Length:264
Mass (Da):29,574
Last modified:February 1, 1995 - v1
Checksum:i280B12748F4488AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08021 mRNA Translation: AAA19904.1
U20971, U20970 Genomic DNA Translation: AAA93158.1
BC000234 mRNA Translation: AAH00234.1
CCDSiCCDS8368.1
PIRiA54060
RefSeqiNP_006160.1, NM_006169.2
UniGeneiHs.503911

Genome annotation databases

EnsembliENST00000299964; ENSP00000299964; ENSG00000166741
ENST00000535401; ENSP00000441434; ENSG00000166741
GeneIDi4837
KEGGihsa:4837

Similar proteinsi

Entry informationi

Entry nameiNNMT_HUMAN
AccessioniPrimary (citable) accession number: P40261
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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