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P40260

- MEP1_YEAST

UniProt

P40260 - MEP1_YEAST

Protein

Ammonium transporter MEP1

Gene

MEP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. Can also transport methylamine. The affinity of MEP1 is about twenty times lower than that of MEP2. MEP3 has the lowest affinity.2 Publications

    GO - Molecular functioni

    1. ammonium transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. ammonium transport Source: SGD
    2. nitrogen utilization Source: SGD

    Keywords - Biological processi

    Ammonia transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30828-MONOMER.

    Protein family/group databases

    TCDBi1.A.11.3.1. the ammonia transporter channel (amt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ammonium transporter MEP1
    Gene namesi
    Name:MEP1
    Synonyms:AMT1
    Ordered Locus Names:YGR121C
    ORF Names:G6331
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR121c.
    SGDiS000003353. MEP1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Ammonium transporter MEP1PRO_0000139754Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei442 – 4421Phosphoserine1 Publication
    Modified residuei445 – 4451Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP40260.
    PeptideAtlasiP40260.

    Expressioni

    Gene expression databases

    GenevestigatoriP40260.

    Interactioni

    Protein-protein interaction databases

    BioGridi33367. 56 interactions.
    DIPiDIP-5530N.
    IntActiP40260. 11 interactions.
    MINTiMINT-475198.
    STRINGi4932.YGR121C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40260.
    SMRiP40260. Positions 20-425.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1818ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini40 – 4910CytoplasmicSequence Analysis
    Topological domaini71 – 10939ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini131 – 14010CytoplasmicSequence Analysis
    Topological domaini162 – 17413ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini196 – 21015CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini232 – 2409ExtracellularSequence Analysis
    Topological domaini262 – 2687CytoplasmicSequence Analysis
    Topological domaini290 – 2901ExtracellularSequence Analysis
    Topological domaini312 – 33120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini353 – 37321ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini395 – 49298CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 3921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei141 – 16121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei269 – 28921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei291 – 31121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei332 – 35221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei374 – 39421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0004.
    GeneTreeiENSGT00530000064546.
    HOGENOMiHOG000017736.
    KOiK03320.
    OMAiESCISIM.
    OrthoDBiEOG79SF67.

    Family and domain databases

    Gene3Di1.10.3430.10. 1 hit.
    InterProiIPR029020. Ammonium/urea_transptr.
    IPR001905. Ammonium_transpt.
    IPR018047. Ammonium_transpt_CS.
    IPR024041. NH4_transpt_AmtB-like_dom.
    [Graphical view]
    PANTHERiPTHR11730. PTHR11730. 1 hit.
    PfamiPF00909. Ammonium_transp. 1 hit.
    [Graphical view]
    SUPFAMiSSF111352. SSF111352. 1 hit.
    TIGRFAMsiTIGR00836. amt. 1 hit.
    PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40260-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL    50
    ALIWVVLMAT LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK 100
    KFDEDAYPEL AYATFQMMFS CVNLSIIAGA TAERGRLLPH MVFLFILATI 150
    GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI EILSAVSGFV YSWFLGKRNE 200
    KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS VYAFMNTCLS 250
    AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ 300
    GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI 350
    GMDGTTEHEG GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG 400
    MRLRISEEAE EAGMDEDQIG EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR 450
    VNNAHLAAER SSSGTNSSSD GNGEMIQSEK ILPIHQEDPA NR 492
    Length:492
    Mass (Da):54,202
    Last modified:February 1, 1995 - v1
    Checksum:iCC7DF0EB838D0B77
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77608 Genomic DNA. Translation: CAA54699.1.
    X83099 Genomic DNA. Translation: CAA58156.1.
    Z72906 Genomic DNA. Translation: CAA97132.1.
    BK006941 Genomic DNA. Translation: DAA08213.1.
    PIRiS46225.
    RefSeqiNP_011636.3. NM_001181250.3.

    Genome annotation databases

    EnsemblFungiiYGR121C; YGR121C; YGR121C.
    GeneIDi853019.
    KEGGisce:YGR121C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77608 Genomic DNA. Translation: CAA54699.1 .
    X83099 Genomic DNA. Translation: CAA58156.1 .
    Z72906 Genomic DNA. Translation: CAA97132.1 .
    BK006941 Genomic DNA. Translation: DAA08213.1 .
    PIRi S46225.
    RefSeqi NP_011636.3. NM_001181250.3.

    3D structure databases

    ProteinModelPortali P40260.
    SMRi P40260. Positions 20-425.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33367. 56 interactions.
    DIPi DIP-5530N.
    IntActi P40260. 11 interactions.
    MINTi MINT-475198.
    STRINGi 4932.YGR121C.

    Protein family/group databases

    TCDBi 1.A.11.3.1. the ammonia transporter channel (amt) family.

    Proteomic databases

    PaxDbi P40260.
    PeptideAtlasi P40260.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR121C ; YGR121C ; YGR121C .
    GeneIDi 853019.
    KEGGi sce:YGR121C.

    Organism-specific databases

    CYGDi YGR121c.
    SGDi S000003353. MEP1.

    Phylogenomic databases

    eggNOGi COG0004.
    GeneTreei ENSGT00530000064546.
    HOGENOMi HOG000017736.
    KOi K03320.
    OMAi ESCISIM.
    OrthoDBi EOG79SF67.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30828-MONOMER.

    Miscellaneous databases

    NextBioi 972889.

    Gene expression databases

    Genevestigatori P40260.

    Family and domain databases

    Gene3Di 1.10.3430.10. 1 hit.
    InterProi IPR029020. Ammonium/urea_transptr.
    IPR001905. Ammonium_transpt.
    IPR018047. Ammonium_transpt_CS.
    IPR024041. NH4_transpt_AmtB-like_dom.
    [Graphical view ]
    PANTHERi PTHR11730. PTHR11730. 1 hit.
    Pfami PF00909. Ammonium_transp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111352. SSF111352. 1 hit.
    TIGRFAMsi TIGR00836. amt. 1 hit.
    PROSITEi PS01219. AMMONIUM_TRANSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae."
      Marini A.-M., Vissers S., Urrestarazu A., Andre B.
      EMBO J. 13:3456-3463(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sigma 1278B.
    2. "An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes."
      van Dyck L., Tettelin H., Purnelle B., Goffeau A.
      Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A family of ammonium transporters in Saccharomyces cerevisiae."
      Marini A.-M., Soussi-Boudekou S., Vissers S., Andre B.
      Mol. Cell. Biol. 17:4282-4293(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: Sigma 1278B.
    6. "Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane."
      Soupene E., Ramirez R.M., Kustu S.
      Mol. Cell. Biol. 21:5733-5741(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442 AND SER-445, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMEP1_YEAST
    AccessioniPrimary (citable) accession number: P40260
    Secondary accession number(s): D6VUQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3