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Protein

Ammonium transporter MEP1

Gene

MEP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. Can also transport methylamine. The affinity of MEP1 is about twenty times lower than that of MEP2. MEP3 has the lowest affinity.2 Publications

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD
  • organic cation transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30828-MONOMER.

Protein family/group databases

TCDBi1.A.11.3.1. the ammonium transporter channel (amt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP1
Gene namesi
Name:MEP1
Synonyms:AMT1
Ordered Locus Names:YGR121C
ORF Names:G6331
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR121C.
SGDiS000003353. MEP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18ExtracellularSequence analysisAdd BLAST18
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Topological domaini40 – 49CytoplasmicSequence analysis10
Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
Topological domaini71 – 109ExtracellularSequence analysisAdd BLAST39
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Topological domaini131 – 140CytoplasmicSequence analysis10
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 174ExtracellularSequence analysisAdd BLAST13
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
Topological domaini196 – 210CytoplasmicSequence analysisAdd BLAST15
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 240ExtracellularSequence analysis9
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Topological domaini262 – 268CytoplasmicSequence analysis7
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290ExtracellularSequence analysis1
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Topological domaini312 – 331CytoplasmicSequence analysisAdd BLAST20
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 373ExtracellularSequence analysisAdd BLAST21
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Topological domaini395 – 492CytoplasmicSequence analysisAdd BLAST98

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397541 – 492Ammonium transporter MEP1Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei442PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP40260.

PTM databases

iPTMnetiP40260.

Interactioni

Protein-protein interaction databases

BioGridi33367. 56 interactors.
DIPiDIP-5530N.
IntActiP40260. 11 interactors.
MINTiMINT-475198.

Structurei

3D structure databases

ProteinModelPortaliP40260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP40260.
KOiK03320.
OMAiWEELAGW.
OrthoDBiEOG092C1Z22.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL
60 70 80 90 100
ALIWVVLMAT LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK
110 120 130 140 150
KFDEDAYPEL AYATFQMMFS CVNLSIIAGA TAERGRLLPH MVFLFILATI
160 170 180 190 200
GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI EILSAVSGFV YSWFLGKRNE
210 220 230 240 250
KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS VYAFMNTCLS
260 270 280 290 300
AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ
310 320 330 340 350
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI
360 370 380 390 400
GMDGTTEHEG GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG
410 420 430 440 450
MRLRISEEAE EAGMDEDQIG EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR
460 470 480 490
VNNAHLAAER SSSGTNSSSD GNGEMIQSEK ILPIHQEDPA NR
Length:492
Mass (Da):54,202
Last modified:February 1, 1995 - v1
Checksum:iCC7DF0EB838D0B77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77608 Genomic DNA. Translation: CAA54699.1.
X83099 Genomic DNA. Translation: CAA58156.1.
Z72906 Genomic DNA. Translation: CAA97132.1.
BK006941 Genomic DNA. Translation: DAA08213.1.
PIRiS46225.
RefSeqiNP_011636.3. NM_001181250.3.

Genome annotation databases

EnsemblFungiiYGR121C; YGR121C; YGR121C.
GeneIDi853019.
KEGGisce:YGR121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77608 Genomic DNA. Translation: CAA54699.1.
X83099 Genomic DNA. Translation: CAA58156.1.
Z72906 Genomic DNA. Translation: CAA97132.1.
BK006941 Genomic DNA. Translation: DAA08213.1.
PIRiS46225.
RefSeqiNP_011636.3. NM_001181250.3.

3D structure databases

ProteinModelPortaliP40260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33367. 56 interactors.
DIPiDIP-5530N.
IntActiP40260. 11 interactors.
MINTiMINT-475198.

Protein family/group databases

TCDBi1.A.11.3.1. the ammonium transporter channel (amt) family.

PTM databases

iPTMnetiP40260.

Proteomic databases

PRIDEiP40260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR121C; YGR121C; YGR121C.
GeneIDi853019.
KEGGisce:YGR121C.

Organism-specific databases

EuPathDBiFungiDB:YGR121C.
SGDiS000003353. MEP1.

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP40260.
KOiK03320.
OMAiWEELAGW.
OrthoDBiEOG092C1Z22.

Enzyme and pathway databases

BioCyciYEAST:G3O-30828-MONOMER.

Miscellaneous databases

PROiP40260.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP1_YEAST
AccessioniPrimary (citable) accession number: P40260
Secondary accession number(s): D6VUQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.