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Protein

Ammonium transporter MEP1

Gene

MEP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. Can also transport methylamine. The affinity of MEP1 is about twenty times lower than that of MEP2. MEP3 has the lowest affinity.2 Publications

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD

Keywordsi

Biological processAmmonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30828-MONOMER

Protein family/group databases

TCDBi1.A.11.3.1 the ammonium transporter channel (amt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP1
Gene namesi
Name:MEP1
Synonyms:AMT1
Ordered Locus Names:YGR121C
ORF Names:G6331
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR121C
SGDiS000003353 MEP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18ExtracellularSequence analysisAdd BLAST18
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Topological domaini40 – 49CytoplasmicSequence analysis10
Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
Topological domaini71 – 109ExtracellularSequence analysisAdd BLAST39
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Topological domaini131 – 140CytoplasmicSequence analysis10
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 174ExtracellularSequence analysisAdd BLAST13
Transmembranei175 – 195HelicalSequence analysisAdd BLAST21
Topological domaini196 – 210CytoplasmicSequence analysisAdd BLAST15
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 240ExtracellularSequence analysis9
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Topological domaini262 – 268CytoplasmicSequence analysis7
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290ExtracellularSequence analysis1
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Topological domaini312 – 331CytoplasmicSequence analysisAdd BLAST20
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 373ExtracellularSequence analysisAdd BLAST21
Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
Topological domaini395 – 492CytoplasmicSequence analysisAdd BLAST98

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397541 – 492Ammonium transporter MEP1Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei442PhosphoserineCombined sources1
Modified residuei445PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP40260
PRIDEiP40260

PTM databases

iPTMnetiP40260

Interactioni

Protein-protein interaction databases

BioGridi33367, 130 interactors
DIPiDIP-5530N
IntActiP40260, 13 interactors
MINTiP40260
STRINGi4932.YGR121C

Structurei

3D structure databases

ProteinModelPortaliP40260
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064546
HOGENOMiHOG000017736
InParanoidiP40260
KOiK03320
OMAiECVNYTG
OrthoDBiEOG092C1Z22

Family and domain databases

Gene3Di1.10.3430.10, 1 hit
InterProiView protein in InterPro
IPR029020 Ammonium/urea_transptr
IPR001905 Ammonium_transpt
IPR018047 Ammonium_transpt_CS
IPR024041 NH4_transpt_AmtB-like_dom
PfamiView protein in Pfam
PF00909 Ammonium_transp, 1 hit
TIGRFAMsiTIGR00836 amt, 1 hit
PROSITEiView protein in PROSITE
PS01219 AMMONIUM_TRANSP, 1 hit

Sequencei

Sequence statusi: Complete.

P40260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL
60 70 80 90 100
ALIWVVLMAT LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK
110 120 130 140 150
KFDEDAYPEL AYATFQMMFS CVNLSIIAGA TAERGRLLPH MVFLFILATI
160 170 180 190 200
GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI EILSAVSGFV YSWFLGKRNE
210 220 230 240 250
KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS VYAFMNTCLS
260 270 280 290 300
AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ
310 320 330 340 350
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI
360 370 380 390 400
GMDGTTEHEG GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG
410 420 430 440 450
MRLRISEEAE EAGMDEDQIG EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR
460 470 480 490
VNNAHLAAER SSSGTNSSSD GNGEMIQSEK ILPIHQEDPA NR
Length:492
Mass (Da):54,202
Last modified:February 1, 1995 - v1
Checksum:iCC7DF0EB838D0B77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77608 Genomic DNA Translation: CAA54699.1
X83099 Genomic DNA Translation: CAA58156.1
Z72906 Genomic DNA Translation: CAA97132.1
BK006941 Genomic DNA Translation: DAA08213.1
PIRiS46225
RefSeqiNP_011636.3, NM_001181250.3

Genome annotation databases

EnsemblFungiiYGR121C; YGR121C; YGR121C
GeneIDi853019
KEGGisce:YGR121C

Similar proteinsi

Entry informationi

Entry nameiMEP1_YEAST
AccessioniPrimary (citable) accession number: P40260
Secondary accession number(s): D6VUQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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