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P40260

- MEP1_YEAST

UniProt

P40260 - MEP1_YEAST

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Protein

Ammonium transporter MEP1

Gene

MEP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. Can also transport methylamine. The affinity of MEP1 is about twenty times lower than that of MEP2. MEP3 has the lowest affinity.2 Publications

GO - Molecular functioni

  1. ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  1. ammonium transport Source: SGD
  2. nitrogen utilization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30828-MONOMER.

Protein family/group databases

TCDBi1.A.11.3.1. the ammonia transporter channel (amt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP1
Gene namesi
Name:MEP1
Synonyms:AMT1
Ordered Locus Names:YGR121C
ORF Names:G6331
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR121c.
SGDiS000003353. MEP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818ExtracellularSequence AnalysisAdd
BLAST
Transmembranei19 – 3921HelicalSequence AnalysisAdd
BLAST
Topological domaini40 – 4910CytoplasmicSequence Analysis
Transmembranei50 – 7021HelicalSequence AnalysisAdd
BLAST
Topological domaini71 – 10939ExtracellularSequence AnalysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Topological domaini131 – 14010CytoplasmicSequence Analysis
Transmembranei141 – 16121HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 17413ExtracellularSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST
Topological domaini196 – 21015CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence AnalysisAdd
BLAST
Topological domaini232 – 2409ExtracellularSequence Analysis
Transmembranei241 – 26121HelicalSequence AnalysisAdd
BLAST
Topological domaini262 – 2687CytoplasmicSequence Analysis
Transmembranei269 – 28921HelicalSequence AnalysisAdd
BLAST
Topological domaini290 – 2901ExtracellularSequence Analysis
Transmembranei291 – 31121HelicalSequence AnalysisAdd
BLAST
Topological domaini312 – 33120CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence AnalysisAdd
BLAST
Topological domaini353 – 37321ExtracellularSequence AnalysisAdd
BLAST
Transmembranei374 – 39421HelicalSequence AnalysisAdd
BLAST
Topological domaini395 – 49298CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Ammonium transporter MEP1PRO_0000139754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei442 – 4421Phosphoserine1 Publication
Modified residuei445 – 4451Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP40260.
PeptideAtlasiP40260.

Expressioni

Gene expression databases

GenevestigatoriP40260.

Interactioni

Protein-protein interaction databases

BioGridi33367. 56 interactions.
DIPiDIP-5530N.
IntActiP40260. 11 interactions.
MINTiMINT-475198.
STRINGi4932.YGR121C.

Structurei

3D structure databases

ProteinModelPortaliP40260.
SMRiP40260. Positions 20-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0004.
GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP40260.
KOiK03320.
OMAiESCISIM.
OrthoDBiEOG79SF67.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40260-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL
60 70 80 90 100
ALIWVVLMAT LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK
110 120 130 140 150
KFDEDAYPEL AYATFQMMFS CVNLSIIAGA TAERGRLLPH MVFLFILATI
160 170 180 190 200
GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI EILSAVSGFV YSWFLGKRNE
210 220 230 240 250
KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS VYAFMNTCLS
260 270 280 290 300
AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ
310 320 330 340 350
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI
360 370 380 390 400
GMDGTTEHEG GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG
410 420 430 440 450
MRLRISEEAE EAGMDEDQIG EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR
460 470 480 490
VNNAHLAAER SSSGTNSSSD GNGEMIQSEK ILPIHQEDPA NR
Length:492
Mass (Da):54,202
Last modified:February 1, 1995 - v1
Checksum:iCC7DF0EB838D0B77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77608 Genomic DNA. Translation: CAA54699.1.
X83099 Genomic DNA. Translation: CAA58156.1.
Z72906 Genomic DNA. Translation: CAA97132.1.
BK006941 Genomic DNA. Translation: DAA08213.1.
PIRiS46225.
RefSeqiNP_011636.3. NM_001181250.3.

Genome annotation databases

EnsemblFungiiYGR121C; YGR121C; YGR121C.
GeneIDi853019.
KEGGisce:YGR121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77608 Genomic DNA. Translation: CAA54699.1 .
X83099 Genomic DNA. Translation: CAA58156.1 .
Z72906 Genomic DNA. Translation: CAA97132.1 .
BK006941 Genomic DNA. Translation: DAA08213.1 .
PIRi S46225.
RefSeqi NP_011636.3. NM_001181250.3.

3D structure databases

ProteinModelPortali P40260.
SMRi P40260. Positions 20-425.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33367. 56 interactions.
DIPi DIP-5530N.
IntActi P40260. 11 interactions.
MINTi MINT-475198.
STRINGi 4932.YGR121C.

Protein family/group databases

TCDBi 1.A.11.3.1. the ammonia transporter channel (amt) family.

Proteomic databases

PaxDbi P40260.
PeptideAtlasi P40260.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR121C ; YGR121C ; YGR121C .
GeneIDi 853019.
KEGGi sce:YGR121C.

Organism-specific databases

CYGDi YGR121c.
SGDi S000003353. MEP1.

Phylogenomic databases

eggNOGi COG0004.
GeneTreei ENSGT00530000064546.
HOGENOMi HOG000017736.
InParanoidi P40260.
KOi K03320.
OMAi ESCISIM.
OrthoDBi EOG79SF67.

Enzyme and pathway databases

BioCyci YEAST:G3O-30828-MONOMER.

Miscellaneous databases

NextBioi 972889.

Gene expression databases

Genevestigatori P40260.

Family and domain databases

Gene3Di 1.10.3430.10. 1 hit.
InterProi IPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view ]
PANTHERi PTHR11730. PTHR11730. 1 hit.
Pfami PF00909. Ammonium_transp. 1 hit.
[Graphical view ]
SUPFAMi SSF111352. SSF111352. 1 hit.
TIGRFAMsi TIGR00836. amt. 1 hit.
PROSITEi PS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae."
    Marini A.-M., Vissers S., Urrestarazu A., Andre B.
    EMBO J. 13:3456-3463(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sigma 1278B.
  2. "An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes."
    van Dyck L., Tettelin H., Purnelle B., Goffeau A.
    Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A family of ammonium transporters in Saccharomyces cerevisiae."
    Marini A.-M., Soussi-Boudekou S., Vissers S., Andre B.
    Mol. Cell. Biol. 17:4282-4293(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: Sigma 1278B.
  6. "Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane."
    Soupene E., Ramirez R.M., Kustu S.
    Mol. Cell. Biol. 21:5733-5741(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442 AND SER-445, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMEP1_YEAST
AccessioniPrimary (citable) accession number: P40260
Secondary accession number(s): D6VUQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3