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P40240

- CD9_MOUSE

UniProt

P40240 - CD9_MOUSE

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Protein

CD9 antigen

Gene

Cd9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17.By similarity3 Publications

GO - Biological processi

  1. brain development Source: Ensembl
  2. cell adhesion Source: UniProtKB
  3. cellular component movement Source: UniProtKB
  4. fusion of sperm to egg plasma membrane Source: UniProtKB
  5. negative regulation of cell proliferation Source: MGI
  6. oligodendrocyte development Source: Ensembl
  7. paranodal junction assembly Source: UniProtKB
  8. response to water deprivation Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Fertilization

Enzyme and pathway databases

ReactomeiREACT_199081. Sperm:Oocyte Membrane Binding.

Names & Taxonomyi

Protein namesi
Recommended name:
CD9 antigen
Alternative name(s):
CD_antigen: CD9
Gene namesi
Name:Cd9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:88348. Cd9.

Subcellular locationi

Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Topological domaini34 – 5320ExtracellularSequence AnalysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Topological domaini75 – 8511CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei86 – 10924HelicalSequence AnalysisAdd
BLAST
Topological domaini110 – 19384ExtracellularSequence AnalysisAdd
BLAST
Transmembranei194 – 21926HelicalSequence AnalysisAdd
BLAST
Topological domaini220 – 2267CytoplasmicSequence Analysis

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. external side of plasma membrane Source: MGI
  3. extracellular space Source: Ensembl
  4. extracellular vesicular exosome Source: Ensembl
  5. integral component of membrane Source: UniProtKB-KW
  6. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi173 – 1753SFQ → AAA: Sperm-egg fusion abolished. 1 Publication
Mutagenesisi174 – 1741F → A: 4-fold reduction of sperm-egg fusion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 226225CD9 antigenPRO_0000219206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi9 – 91S-palmitoyl cysteineBy similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Lipidationi76 – 761S-palmitoyl cysteineBy similarity
Lipidationi77 – 771S-palmitoyl cysteineBy similarity
Lipidationi85 – 851S-palmitoyl cysteineBy similarity
Lipidationi216 – 2161S-palmitoyl cysteineBy similarity
Lipidationi217 – 2171S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP40240.
PaxDbiP40240.
PRIDEiP40240.

PTM databases

PhosphoSiteiP40240.

Expressioni

Tissue specificityi

Expressed predominantly in the peripheral nervous system.1 Publication

Gene expression databases

BgeeiP40240.
CleanExiMM_CD9.
GenevestigatoriP40240.

Interactioni

Subunit structurei

Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8 (By similarity).By similarity

Protein-protein interaction databases

IntActiP40240. 1 interaction.
MINTiMINT-4090327.
STRINGi10090.ENSMUSP00000032492.

Structurei

3D structure databases

ProteinModelPortaliP40240.
SMRiP40240. Positions 108-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG242618.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP40240.
KOiK06460.
OMAiALNCCGI.
OrthoDBiEOG7FBRJV.
PhylomeDBiP40240.
TreeFamiTF352895.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40240-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVKGGSKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQENN
60 70 80 90 100
HSSFYTGVYI LIGAGALMML VGFLGCCGAV QESQCMLGLF FGFLLVIFAI
110 120 130 140 150
EIAAAVWGYT HKDEVIKELQ EFYKDTYQKL RSKDEPQRET LKAIHMALDC
160 170 180 190 200
CGIAGPLEQF ISDTCPKKQL LESFQVKPCP EAISEVFNNK FHIIGAVGIG
210 220
IAVVMIFGMI FSMILCCAIR RSREMV
Length:226
Mass (Da):25,258
Last modified:January 23, 2007 - v2
Checksum:i06D24A878BF348C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA. Translation: AAA37405.1.
AK002251 mRNA. Translation: BAB21965.1.
AK012793 mRNA. Translation: BAB28473.1.
AK151443 mRNA. Translation: BAE30405.1.
AK151619 mRNA. Translation: BAE30556.1.
AK170310 mRNA. Translation: BAE41707.1.
BC070474 mRNA. Translation: AAH70474.1.
CCDSiCCDS20551.1.
PIRiI49589.
RefSeqiNP_031683.1. NM_007657.3.
UniGeneiMm.210676.
Mm.404614.

Genome annotation databases

EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
GeneIDi12527.
KEGGimmu:12527.
UCSCiuc009dun.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA. Translation: AAA37405.1 .
AK002251 mRNA. Translation: BAB21965.1 .
AK012793 mRNA. Translation: BAB28473.1 .
AK151443 mRNA. Translation: BAE30405.1 .
AK151619 mRNA. Translation: BAE30556.1 .
AK170310 mRNA. Translation: BAE41707.1 .
BC070474 mRNA. Translation: AAH70474.1 .
CCDSi CCDS20551.1.
PIRi I49589.
RefSeqi NP_031683.1. NM_007657.3.
UniGenei Mm.210676.
Mm.404614.

3D structure databases

ProteinModelPortali P40240.
SMRi P40240. Positions 108-189.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P40240. 1 interaction.
MINTi MINT-4090327.
STRINGi 10090.ENSMUSP00000032492.

PTM databases

PhosphoSitei P40240.

Proteomic databases

MaxQBi P40240.
PaxDbi P40240.
PRIDEi P40240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032492 ; ENSMUSP00000032492 ; ENSMUSG00000030342 .
GeneIDi 12527.
KEGGi mmu:12527.
UCSCi uc009dun.1. mouse.

Organism-specific databases

CTDi 928.
MGIi MGI:88348. Cd9.

Phylogenomic databases

eggNOGi NOG242618.
GeneTreei ENSGT00760000118983.
HOGENOMi HOG000230651.
HOVERGENi HBG002324.
InParanoidi P40240.
KOi K06460.
OMAi ALNCCGI.
OrthoDBi EOG7FBRJV.
PhylomeDBi P40240.
TreeFami TF352895.

Enzyme and pathway databases

Reactomei REACT_199081. Sperm:Oocyte Membrane Binding.

Miscellaneous databases

NextBioi 281554.
PROi P40240.
SOURCEi Search...

Gene expression databases

Bgeei P40240.
CleanExi MM_CD9.
Genevestigatori P40240.

Family and domain databases

Gene3Di 1.10.1450.10. 1 hit.
InterProi IPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view ]
Pfami PF00335. Tetraspannin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002419. Tetraspanin. 1 hit.
PRINTSi PR00259. TMFOUR.
SUPFAMi SSF48652. SSF48652. 1 hit.
PROSITEi PS00421. TM4_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the mouse equivalent of CD9 antigen."
    Rubinstein E., Billard M., Plaisance S., Prenant M., Boucheix C.
    Thromb. Res. 71:377-383(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "The gamete fusion process is defective in eggs of Cd9-deficient mice."
    Kaji K., Oda S., Shikano T., Ohnuki T., Uematsu Y., Sakagami J., Tada N., Miyazaki S., Kudo A.
    Nat. Genet. 24:279-282(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN GAMETE FUSION.
  5. "Residues SFQ (173-175) in the large extracellular loop of CD9 are required for gamete fusion."
    Zhu G.-Z., Miller B.J., Boucheix C., Rubinstein E., Liu C.C., Hynes R.O., Myles D.G., Primakoff P.
    Development 129:1995-2002(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PHE-174 AND 173-SER--GLN-175.
  6. "Murine CD9 is the receptor for pregnancy-specific glycoprotein 17."
    Waterhouse R., Ha C., Dveksler G.S.
    J. Exp. Med. 195:277-282(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A RECEPTOR FOR PSG17, SUBCELLULAR LOCATION.
  7. "Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation."
    Ishibashi T., Ding L., Ikenaka K., Inoue Y., Miyado K., Mekada E., Baba H.
    J. Neurosci. 24:96-102(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN PARANODAL FORMATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCD9_MOUSE
AccessioniPrimary (citable) accession number: P40240
Secondary accession number(s): Q3U9W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3