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Protein

CD9 antigen

Gene

Cd9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17.By similarity3 Publications

GO - Biological processi

  • brain development Source: Ensembl
  • cell adhesion Source: UniProtKB
  • cell surface receptor signaling pathway Source: GO_Central
  • cellular response to low-density lipoprotein particle stimulus Source: UniProtKB
  • fusion of sperm to egg plasma membrane Source: UniProtKB
  • movement of cell or subcellular component Source: UniProtKB
  • negative regulation of cell proliferation Source: MGI
  • oligodendrocyte development Source: Ensembl
  • paranodal junction assembly Source: UniProtKB
  • receptor internalization Source: UniProtKB
  • response to water deprivation Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Fertilization

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-1300652. Sperm:Oocyte Membrane Binding.

Names & Taxonomyi

Protein namesi
Recommended name:
CD9 antigen
Alternative name(s):
CD_antigen: CD9
Gene namesi
Name:Cd9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88348. Cd9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 12CytoplasmicSequence analysisAdd BLAST11
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 53ExtracellularSequence analysisAdd BLAST20
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 85CytoplasmicSequence analysisAdd BLAST11
Transmembranei86 – 109HelicalSequence analysisAdd BLAST24
Topological domaini110 – 193ExtracellularSequence analysisAdd BLAST84
Transmembranei194 – 219HelicalSequence analysisAdd BLAST26
Topological domaini220 – 226CytoplasmicSequence analysis7

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • extracellular vesicle Source: MGI
  • focal adhesion Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173 – 175SFQ → AAA: Sperm-egg fusion abolished. 1 Publication3
Mutagenesisi174F → A: 4-fold reduction of sperm-egg fusion. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002192062 – 226CD9 antigenAdd BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi9S-palmitoyl cysteineBy similarity1
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
Lipidationi76S-palmitoyl cysteineBy similarity1
Lipidationi77S-palmitoyl cysteineBy similarity1
Lipidationi85S-palmitoyl cysteineBy similarity1
Lipidationi216S-palmitoyl cysteineBy similarity1
Lipidationi217S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP40240.
PaxDbiP40240.
PeptideAtlasiP40240.
PRIDEiP40240.

PTM databases

PhosphoSitePlusiP40240.
SwissPalmiP40240.

Expressioni

Tissue specificityi

Expressed predominantly in the peripheral nervous system.1 Publication

Gene expression databases

BgeeiENSMUSG00000030342.
CleanExiMM_CD9.
GenevisibleiP40240. MM.

Interactioni

Subunit structurei

Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8 (By similarity).By similarity

Protein-protein interaction databases

IntActiP40240. 1 interactor.
MINTiMINT-4090327.
STRINGi10090.ENSMUSP00000032492.

Structurei

3D structure databases

ProteinModelPortaliP40240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP40240.
KOiK06460.
OMAiHFALNCC.
OrthoDBiEOG091G0K9H.
PhylomeDBiP40240.
TreeFamiTF352895.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKGGSKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQENN
60 70 80 90 100
HSSFYTGVYI LIGAGALMML VGFLGCCGAV QESQCMLGLF FGFLLVIFAI
110 120 130 140 150
EIAAAVWGYT HKDEVIKELQ EFYKDTYQKL RSKDEPQRET LKAIHMALDC
160 170 180 190 200
CGIAGPLEQF ISDTCPKKQL LESFQVKPCP EAISEVFNNK FHIIGAVGIG
210 220
IAVVMIFGMI FSMILCCAIR RSREMV
Length:226
Mass (Da):25,258
Last modified:January 23, 2007 - v2
Checksum:i06D24A878BF348C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA. Translation: AAA37405.1.
AK002251 mRNA. Translation: BAB21965.1.
AK012793 mRNA. Translation: BAB28473.1.
AK151443 mRNA. Translation: BAE30405.1.
AK151619 mRNA. Translation: BAE30556.1.
AK170310 mRNA. Translation: BAE41707.1.
BC070474 mRNA. Translation: AAH70474.1.
CCDSiCCDS20551.1.
PIRiI49589.
RefSeqiNP_031683.1. NM_007657.3.
UniGeneiMm.210676.
Mm.404614.

Genome annotation databases

EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
GeneIDi12527.
KEGGimmu:12527.
UCSCiuc009dun.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA. Translation: AAA37405.1.
AK002251 mRNA. Translation: BAB21965.1.
AK012793 mRNA. Translation: BAB28473.1.
AK151443 mRNA. Translation: BAE30405.1.
AK151619 mRNA. Translation: BAE30556.1.
AK170310 mRNA. Translation: BAE41707.1.
BC070474 mRNA. Translation: AAH70474.1.
CCDSiCCDS20551.1.
PIRiI49589.
RefSeqiNP_031683.1. NM_007657.3.
UniGeneiMm.210676.
Mm.404614.

3D structure databases

ProteinModelPortaliP40240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP40240. 1 interactor.
MINTiMINT-4090327.
STRINGi10090.ENSMUSP00000032492.

PTM databases

PhosphoSitePlusiP40240.
SwissPalmiP40240.

Proteomic databases

MaxQBiP40240.
PaxDbiP40240.
PeptideAtlasiP40240.
PRIDEiP40240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
GeneIDi12527.
KEGGimmu:12527.
UCSCiuc009dun.1. mouse.

Organism-specific databases

CTDi928.
MGIiMGI:88348. Cd9.

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP40240.
KOiK06460.
OMAiHFALNCC.
OrthoDBiEOG091G0K9H.
PhylomeDBiP40240.
TreeFamiTF352895.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-1300652. Sperm:Oocyte Membrane Binding.

Miscellaneous databases

PROiP40240.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030342.
CleanExiMM_CD9.
GenevisibleiP40240. MM.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD9_MOUSE
AccessioniPrimary (citable) accession number: P40240
Secondary accession number(s): Q3U9W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.