Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CD9 antigen

Gene

Cd9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17.By similarity3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Fertilization

Enzyme and pathway databases

ReactomeiREACT_286266. Sperm:Oocyte Membrane Binding.
REACT_307071. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
CD9 antigen
Alternative name(s):
CD_antigen: CD9
Gene namesi
Name:Cd9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88348. Cd9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Topological domaini34 – 5320ExtracellularSequence AnalysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Topological domaini75 – 8511CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei86 – 10924HelicalSequence AnalysisAdd
BLAST
Topological domaini110 – 19384ExtracellularSequence AnalysisAdd
BLAST
Transmembranei194 – 21926HelicalSequence AnalysisAdd
BLAST
Topological domaini220 – 2267CytoplasmicSequence Analysis

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • extracellular vesicle Source: MGI
  • focal adhesion Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi173 – 1753SFQ → AAA: Sperm-egg fusion abolished. 1 Publication
Mutagenesisi174 – 1741F → A: 4-fold reduction of sperm-egg fusion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 226225CD9 antigenPRO_0000219206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi9 – 91S-palmitoyl cysteineBy similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Lipidationi76 – 761S-palmitoyl cysteineBy similarity
Lipidationi77 – 771S-palmitoyl cysteineBy similarity
Lipidationi85 – 851S-palmitoyl cysteineBy similarity
Lipidationi216 – 2161S-palmitoyl cysteineBy similarity
Lipidationi217 – 2171S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP40240.
PaxDbiP40240.
PRIDEiP40240.

PTM databases

PhosphoSiteiP40240.

Expressioni

Tissue specificityi

Expressed predominantly in the peripheral nervous system.1 Publication

Gene expression databases

BgeeiP40240.
CleanExiMM_CD9.
GenevisibleiP40240. MM.

Interactioni

Subunit structurei

Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8 (By similarity).By similarity

Protein-protein interaction databases

IntActiP40240. 1 interaction.
MINTiMINT-4090327.
STRINGi10090.ENSMUSP00000032492.

Structurei

3D structure databases

ProteinModelPortaliP40240.
SMRiP40240. Positions 109-190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG242618.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP40240.
KOiK06460.
OMAiDCCGLAG.
OrthoDBiEOG7FBRJV.
PhylomeDBiP40240.
TreeFamiTF352895.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKGGSKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQENN
60 70 80 90 100
HSSFYTGVYI LIGAGALMML VGFLGCCGAV QESQCMLGLF FGFLLVIFAI
110 120 130 140 150
EIAAAVWGYT HKDEVIKELQ EFYKDTYQKL RSKDEPQRET LKAIHMALDC
160 170 180 190 200
CGIAGPLEQF ISDTCPKKQL LESFQVKPCP EAISEVFNNK FHIIGAVGIG
210 220
IAVVMIFGMI FSMILCCAIR RSREMV
Length:226
Mass (Da):25,258
Last modified:January 23, 2007 - v2
Checksum:i06D24A878BF348C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA. Translation: AAA37405.1.
AK002251 mRNA. Translation: BAB21965.1.
AK012793 mRNA. Translation: BAB28473.1.
AK151443 mRNA. Translation: BAE30405.1.
AK151619 mRNA. Translation: BAE30556.1.
AK170310 mRNA. Translation: BAE41707.1.
BC070474 mRNA. Translation: AAH70474.1.
CCDSiCCDS20551.1.
PIRiI49589.
RefSeqiNP_031683.1. NM_007657.3.
UniGeneiMm.210676.
Mm.404614.

Genome annotation databases

EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
GeneIDi12527.
KEGGimmu:12527.
UCSCiuc009dun.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA. Translation: AAA37405.1.
AK002251 mRNA. Translation: BAB21965.1.
AK012793 mRNA. Translation: BAB28473.1.
AK151443 mRNA. Translation: BAE30405.1.
AK151619 mRNA. Translation: BAE30556.1.
AK170310 mRNA. Translation: BAE41707.1.
BC070474 mRNA. Translation: AAH70474.1.
CCDSiCCDS20551.1.
PIRiI49589.
RefSeqiNP_031683.1. NM_007657.3.
UniGeneiMm.210676.
Mm.404614.

3D structure databases

ProteinModelPortaliP40240.
SMRiP40240. Positions 109-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP40240. 1 interaction.
MINTiMINT-4090327.
STRINGi10090.ENSMUSP00000032492.

PTM databases

PhosphoSiteiP40240.

Proteomic databases

MaxQBiP40240.
PaxDbiP40240.
PRIDEiP40240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
GeneIDi12527.
KEGGimmu:12527.
UCSCiuc009dun.1. mouse.

Organism-specific databases

CTDi928.
MGIiMGI:88348. Cd9.

Phylogenomic databases

eggNOGiNOG242618.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP40240.
KOiK06460.
OMAiDCCGLAG.
OrthoDBiEOG7FBRJV.
PhylomeDBiP40240.
TreeFamiTF352895.

Enzyme and pathway databases

ReactomeiREACT_286266. Sperm:Oocyte Membrane Binding.
REACT_307071. Platelet degranulation.

Miscellaneous databases

NextBioi281554.
PROiP40240.
SOURCEiSearch...

Gene expression databases

BgeeiP40240.
CleanExiMM_CD9.
GenevisibleiP40240. MM.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the mouse equivalent of CD9 antigen."
    Rubinstein E., Billard M., Plaisance S., Prenant M., Boucheix C.
    Thromb. Res. 71:377-383(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "The gamete fusion process is defective in eggs of Cd9-deficient mice."
    Kaji K., Oda S., Shikano T., Ohnuki T., Uematsu Y., Sakagami J., Tada N., Miyazaki S., Kudo A.
    Nat. Genet. 24:279-282(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN GAMETE FUSION.
  5. "Residues SFQ (173-175) in the large extracellular loop of CD9 are required for gamete fusion."
    Zhu G.-Z., Miller B.J., Boucheix C., Rubinstein E., Liu C.C., Hynes R.O., Myles D.G., Primakoff P.
    Development 129:1995-2002(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PHE-174 AND 173-SER--GLN-175.
  6. "Murine CD9 is the receptor for pregnancy-specific glycoprotein 17."
    Waterhouse R., Ha C., Dveksler G.S.
    J. Exp. Med. 195:277-282(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A RECEPTOR FOR PSG17, SUBCELLULAR LOCATION.
  7. "Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation."
    Ishibashi T., Ding L., Ikenaka K., Inoue Y., Miyado K., Mekada E., Baba H.
    J. Neurosci. 24:96-102(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN PARANODAL FORMATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCD9_MOUSE
AccessioniPrimary (citable) accession number: P40240
Secondary accession number(s): Q3U9W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.