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Protein

CD9 antigen

Gene

Cd9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17.By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion, Fertilization

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1300652 Sperm:Oocyte Membrane Binding

Names & Taxonomyi

Protein namesi
Recommended name:
CD9 antigen
Alternative name(s):
CD_antigen: CD9
Gene namesi
Name:Cd9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88348 Cd9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 53ExtracellularSequence analysisAdd BLAST20
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 85CytoplasmicSequence analysisAdd BLAST11
Transmembranei86 – 109HelicalSequence analysisAdd BLAST24
Topological domaini110 – 193ExtracellularSequence analysisAdd BLAST84
Transmembranei194 – 219HelicalSequence analysisAdd BLAST26
Topological domaini220 – 226CytoplasmicSequence analysis7

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173 – 175SFQ → AAA: Sperm-egg fusion abolished. 1 Publication3
Mutagenesisi174F → A: 4-fold reduction of sperm-egg fusion. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002192061 – 226CD9 antigenAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi9S-palmitoyl cysteineBy similarity1
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi76S-palmitoyl cysteineBy similarity1
Lipidationi77S-palmitoyl cysteineBy similarity1
Lipidationi85S-palmitoyl cysteineBy similarity1
Disulfide bondi150 ↔ 179PROSITE-ProRule annotation
Disulfide bondi151 ↔ 165PROSITE-ProRule annotation
Lipidationi216S-palmitoyl cysteineBy similarity1
Lipidationi217S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP40240
PaxDbiP40240
PeptideAtlasiP40240
PRIDEiP40240

PTM databases

PhosphoSitePlusiP40240
SwissPalmiP40240

Expressioni

Tissue specificityi

Expressed predominantly in the peripheral nervous system.1 Publication

Gene expression databases

BgeeiENSMUSG00000030342
CleanExiMM_CD9
GenevisibleiP40240 MM

Interactioni

Subunit structurei

Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Interacts with PDPN; this interaction is homophilic and attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP40240, 1 interactor
MINTiP40240
STRINGi10090.ENSMUSP00000032492

Structurei

3D structure databases

ProteinModelPortaliP40240
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882 Eukaryota
ENOG4111IRY LUCA
GeneTreeiENSGT00880000137858
HOGENOMiHOG000230651
HOVERGENiHBG002324
InParanoidiP40240
KOiK06460
OMAiEVFHNKF
OrthoDBiEOG091G0K9H
PhylomeDBiP40240
TreeFamiTF352895

Family and domain databases

Gene3Di1.10.1450.10, 1 hit
InterProiView protein in InterPro
IPR000301 Tetraspanin
IPR018499 Tetraspanin/Peripherin
IPR018503 Tetraspanin_CS
IPR008952 Tetraspanin_EC2_sf
PfamiView protein in Pfam
PF00335 Tetraspannin, 1 hit
PIRSFiPIRSF002419 Tetraspanin, 1 hit
PRINTSiPR00259 TMFOUR
SUPFAMiSSF48652 SSF48652, 1 hit
PROSITEiView protein in PROSITE
PS00421 TM4_1, 1 hit

Sequencei

Sequence statusi: Complete.

P40240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKGGSKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQENN
60 70 80 90 100
HSSFYTGVYI LIGAGALMML VGFLGCCGAV QESQCMLGLF FGFLLVIFAI
110 120 130 140 150
EIAAAVWGYT HKDEVIKELQ EFYKDTYQKL RSKDEPQRET LKAIHMALDC
160 170 180 190 200
CGIAGPLEQF ISDTCPKKQL LESFQVKPCP EAISEVFNNK FHIIGAVGIG
210 220
IAVVMIFGMI FSMILCCAIR RSREMV
Length:226
Mass (Da):25,258
Last modified:January 23, 2007 - v2
Checksum:i06D24A878BF348C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08115 mRNA Translation: AAA37405.1
AK002251 mRNA Translation: BAB21965.1
AK012793 mRNA Translation: BAB28473.1
AK151443 mRNA Translation: BAE30405.1
AK151619 mRNA Translation: BAE30556.1
AK170310 mRNA Translation: BAE41707.1
BC070474 mRNA Translation: AAH70474.1
CCDSiCCDS20551.1
PIRiI49589
RefSeqiNP_031683.1, NM_007657.3
UniGeneiMm.210676
Mm.404614

Genome annotation databases

EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342
GeneIDi12527
KEGGimmu:12527
UCSCiuc009dun.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCD9_MOUSE
AccessioniPrimary (citable) accession number: P40240
Secondary accession number(s): Q3U9W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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