P40240 (CD9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD9 antigen

Alternative name(s):
CD_antigen=CD9

Gene names

Name:

Cd9

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion By similarity. Acts as a receptor for PSG17. Ref.4 Ref.6 Ref.7
Subunit structure	Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed predominantly in the peripheral nervous system. Ref.7
Post-translational modification	Phosphorylated on tyrosine following B-cell activation By similarity.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Biological process	Cell adhesion Fertilization
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from electronic annotation. Source: Compara cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB cellular component movement Inferred from sequence or structural similarity. Source: UniProtKB fusion of sperm to egg plasma membrane Inferred from direct assay Ref.4. Source: UniProtKB negative regulation of cell proliferation Inferred from direct assay PubMed 7650394. Source: MGI oligodendrocyte development Inferred from electronic annotation. Source: Compara paranodal junction assembly Inferred from direct assay Ref.7. Source: UniProtKB response to water deprivation Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from direct assay PubMed 15199151. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 226	225	CD9 antigen		PRO_0000219206
Regions
	Topological domain	2 – 12	11	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Potential
	Topological domain	34 – 53	20	Extracellular Potential
	Transmembrane	54 – 74	21	Helical; Potential
	Topological domain	75 – 85	11	Cytoplasmic Potential
	Transmembrane	86 – 109	24	Helical; Potential
	Topological domain	110 – 193	84	Extracellular Potential
	Transmembrane	194 – 219	26	Helical; Potential
	Topological domain	220 – 226	7	Cytoplasmic Potential
Amino acid modifications
	Lipidation	9	1	S-palmitoyl cysteine By similarity
	Lipidation	76	1	S-palmitoyl cysteine By similarity
	Lipidation	77	1	S-palmitoyl cysteine By similarity
	Lipidation	85	1	S-palmitoyl cysteine By similarity
	Lipidation	216	1	S-palmitoyl cysteine By similarity
	Lipidation	217	1	S-palmitoyl cysteine By similarity
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	173 – 175	3	SFQ → AAA: Sperm-egg fusion abolished. Ref.5
	Mutagenesis	174	1	F → A: 4-fold reduction of sperm-egg fusion. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P40240 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 06D24A878BF348C5	FASTA	226	25,258
	10 20 30 40 50 60 MPVKGGSKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQENN HSSFYTGVYI 70 80 90 100 110 120 LIGAGALMML VGFLGCCGAV QESQCMLGLF FGFLLVIFAI EIAAAVWGYT HKDEVIKELQ 130 140 150 160 170 180 EFYKDTYQKL RSKDEPQRET LKAIHMALDC CGIAGPLEQF ISDTCPKKQL LESFQVKPCP 190 200 210 220 EAISEVFNNK FHIIGAVGIG IAVVMIFGMI FSMILCCAIR RSREMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the mouse equivalent of CD9 antigen." Rubinstein E., Billard M., Plaisance S., Prenant M., Boucheix C. Thromb. Res. 71:377-383(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Kidney.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"The gamete fusion process is defective in eggs of Cd9-deficient mice." Kaji K., Oda S., Shikano T., Ohnuki T., Uematsu Y., Sakagami J., Tada N., Miyazaki S., Kudo A. Nat. Genet. 24:279-282(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN GAMETE FUSION.
[5]	"Residues SFQ (173-175) in the large extracellular loop of CD9 are required for gamete fusion." Zhu G.-Z., Miller B.J., Boucheix C., Rubinstein E., Liu C.C., Hynes R.O., Myles D.G., Primakoff P. Development 129:1995-2002(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF PHE-174 AND 173-SER--GLN-175.
[6]	"Murine CD9 is the receptor for pregnancy-specific glycoprotein 17." Waterhouse R., Ha C., Dveksler G.S. J. Exp. Med. 195:277-282(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A RECEPTOR FOR PSG17.
[7]	"Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation." Ishibashi T., Ding L., Ikenaka K., Inoue Y., Miyado K., Mekada E., Baba H. J. Neurosci. 24:96-102(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN PARANODAL FORMATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08115 mRNA. Translation: AAA37405.1. AK002251 mRNA. Translation: BAB21965.1. AK012793 mRNA. Translation: BAB28473.1. AK151443 mRNA. Translation: BAE30405.1. AK151619 mRNA. Translation: BAE30556.1. AK170310 mRNA. Translation: BAE41707.1. BC070474 mRNA. Translation: AAH70474.1.
IPI	IPI00221921.
PIR	I49589.
RefSeq	NP_031683.1. NM_007657.3.
UniGene	Mm.210676. Mm.404614.
3D structure databases
ProteinModelPortal	P40240.
SMR	P40240. Positions 109-189.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032492.
PTM databases
PhosphoSite	P40240.
Proteomic databases
PaxDb	P40240.
PRIDE	P40240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
GeneID	12527.
KEGG	mmu:12527.
UCSC	uc009dun.1. mouse.
Organism-specific databases
CTD	928.
MGI	MGI:88348. Cd9.
Phylogenomic databases
eggNOG	NOG242618.
GeneTree	ENSGT00680000099842.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	P40240.
KO	K06460.
OMA	VKPCPEA.
OrthoDB	EOG402WT9.
Gene expression databases
Bgee	P40240.
CleanEx	MM_CD9.
Genevestigator	P40240.
GermOnline	ENSMUSG00000030342. Mus musculus.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281554.
SOURCE	Search...

Entry information

Entry name

CD9_MOUSE

Accession

Primary (citable) accession number: P40240
Secondary accession number(s): Q3U9W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program