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P40240

- CD9_MOUSE

UniProt

P40240 - CD9_MOUSE

Protein

CD9 antigen

Gene

Cd9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion By similarity. Acts as a receptor for PSG17.By similarity3 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. brain development Source: Ensembl
    2. cell adhesion Source: UniProtKB
    3. cellular component movement Source: UniProtKB
    4. fusion of sperm to egg plasma membrane Source: UniProtKB
    5. negative regulation of cell proliferation Source: MGI
    6. oligodendrocyte development Source: Ensembl
    7. paranodal junction assembly Source: UniProtKB
    8. response to water deprivation Source: Ensembl

    Keywords - Biological processi

    Cell adhesion, Fertilization

    Enzyme and pathway databases

    ReactomeiREACT_199081. Sperm:Oocyte Membrane Binding.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD9 antigen
    Alternative name(s):
    CD_antigen: CD9
    Gene namesi
    Name:Cd9
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:88348. Cd9.

    Subcellular locationi

    Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. external side of plasma membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi173 – 1753SFQ → AAA: Sperm-egg fusion abolished.
    Mutagenesisi174 – 1741F → A: 4-fold reduction of sperm-egg fusion. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 226225CD9 antigenPRO_0000219206Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi9 – 91S-palmitoyl cysteineBy similarity
    Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
    Lipidationi76 – 761S-palmitoyl cysteineBy similarity
    Lipidationi77 – 771S-palmitoyl cysteineBy similarity
    Lipidationi85 – 851S-palmitoyl cysteineBy similarity
    Lipidationi216 – 2161S-palmitoyl cysteineBy similarity
    Lipidationi217 – 2171S-palmitoyl cysteineBy similarity

    Post-translational modificationi

    Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP40240.
    PRIDEiP40240.

    PTM databases

    PhosphoSiteiP40240.

    Expressioni

    Tissue specificityi

    Expressed predominantly in the peripheral nervous system.1 Publication

    Gene expression databases

    BgeeiP40240.
    CleanExiMM_CD9.
    GenevestigatoriP40240.

    Interactioni

    Subunit structurei

    Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP40240. 1 interaction.
    MINTiMINT-4090327.
    STRINGi10090.ENSMUSP00000032492.

    Structurei

    3D structure databases

    ProteinModelPortaliP40240.
    SMRiP40240. Positions 109-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 5320ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini75 – 8511CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini110 – 19384ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini220 – 2267CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei86 – 10924HelicalSequence AnalysisAdd
    BLAST
    Transmembranei194 – 21926HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tetraspanin (TM4SF) family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG242618.
    GeneTreeiENSGT00680000099842.
    HOGENOMiHOG000230651.
    HOVERGENiHBG002324.
    InParanoidiP40240.
    KOiK06460.
    OMAiALNCCGI.
    OrthoDBiEOG7FBRJV.
    PhylomeDBiP40240.
    TreeFamiTF352895.

    Family and domain databases

    Gene3Di1.10.1450.10. 1 hit.
    InterProiIPR028977. CD81_LEL.
    IPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view]
    PfamiPF00335. Tetraspannin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002419. Tetraspanin. 1 hit.
    PRINTSiPR00259. TMFOUR.
    SUPFAMiSSF48652. SSF48652. 1 hit.
    PROSITEiPS00421. TM4_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVKGGSKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQENN    50
    HSSFYTGVYI LIGAGALMML VGFLGCCGAV QESQCMLGLF FGFLLVIFAI 100
    EIAAAVWGYT HKDEVIKELQ EFYKDTYQKL RSKDEPQRET LKAIHMALDC 150
    CGIAGPLEQF ISDTCPKKQL LESFQVKPCP EAISEVFNNK FHIIGAVGIG 200
    IAVVMIFGMI FSMILCCAIR RSREMV 226
    Length:226
    Mass (Da):25,258
    Last modified:January 23, 2007 - v2
    Checksum:i06D24A878BF348C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08115 mRNA. Translation: AAA37405.1.
    AK002251 mRNA. Translation: BAB21965.1.
    AK012793 mRNA. Translation: BAB28473.1.
    AK151443 mRNA. Translation: BAE30405.1.
    AK151619 mRNA. Translation: BAE30556.1.
    AK170310 mRNA. Translation: BAE41707.1.
    BC070474 mRNA. Translation: AAH70474.1.
    CCDSiCCDS20551.1.
    PIRiI49589.
    RefSeqiNP_031683.1. NM_007657.3.
    UniGeneiMm.210676.
    Mm.404614.

    Genome annotation databases

    EnsembliENSMUST00000032492; ENSMUSP00000032492; ENSMUSG00000030342.
    GeneIDi12527.
    KEGGimmu:12527.
    UCSCiuc009dun.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08115 mRNA. Translation: AAA37405.1 .
    AK002251 mRNA. Translation: BAB21965.1 .
    AK012793 mRNA. Translation: BAB28473.1 .
    AK151443 mRNA. Translation: BAE30405.1 .
    AK151619 mRNA. Translation: BAE30556.1 .
    AK170310 mRNA. Translation: BAE41707.1 .
    BC070474 mRNA. Translation: AAH70474.1 .
    CCDSi CCDS20551.1.
    PIRi I49589.
    RefSeqi NP_031683.1. NM_007657.3.
    UniGenei Mm.210676.
    Mm.404614.

    3D structure databases

    ProteinModelPortali P40240.
    SMRi P40240. Positions 109-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P40240. 1 interaction.
    MINTi MINT-4090327.
    STRINGi 10090.ENSMUSP00000032492.

    PTM databases

    PhosphoSitei P40240.

    Proteomic databases

    PaxDbi P40240.
    PRIDEi P40240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032492 ; ENSMUSP00000032492 ; ENSMUSG00000030342 .
    GeneIDi 12527.
    KEGGi mmu:12527.
    UCSCi uc009dun.1. mouse.

    Organism-specific databases

    CTDi 928.
    MGIi MGI:88348. Cd9.

    Phylogenomic databases

    eggNOGi NOG242618.
    GeneTreei ENSGT00680000099842.
    HOGENOMi HOG000230651.
    HOVERGENi HBG002324.
    InParanoidi P40240.
    KOi K06460.
    OMAi ALNCCGI.
    OrthoDBi EOG7FBRJV.
    PhylomeDBi P40240.
    TreeFami TF352895.

    Enzyme and pathway databases

    Reactomei REACT_199081. Sperm:Oocyte Membrane Binding.

    Miscellaneous databases

    NextBioi 281554.
    PROi P40240.
    SOURCEi Search...

    Gene expression databases

    Bgeei P40240.
    CleanExi MM_CD9.
    Genevestigatori P40240.

    Family and domain databases

    Gene3Di 1.10.1450.10. 1 hit.
    InterProi IPR028977. CD81_LEL.
    IPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view ]
    Pfami PF00335. Tetraspannin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002419. Tetraspanin. 1 hit.
    PRINTSi PR00259. TMFOUR.
    SUPFAMi SSF48652. SSF48652. 1 hit.
    PROSITEi PS00421. TM4_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the mouse equivalent of CD9 antigen."
      Rubinstein E., Billard M., Plaisance S., Prenant M., Boucheix C.
      Thromb. Res. 71:377-383(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "The gamete fusion process is defective in eggs of Cd9-deficient mice."
      Kaji K., Oda S., Shikano T., Ohnuki T., Uematsu Y., Sakagami J., Tada N., Miyazaki S., Kudo A.
      Nat. Genet. 24:279-282(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN GAMETE FUSION.
    5. "Residues SFQ (173-175) in the large extracellular loop of CD9 are required for gamete fusion."
      Zhu G.-Z., Miller B.J., Boucheix C., Rubinstein E., Liu C.C., Hynes R.O., Myles D.G., Primakoff P.
      Development 129:1995-2002(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF PHE-174 AND 173-SER--GLN-175.
    6. "Murine CD9 is the receptor for pregnancy-specific glycoprotein 17."
      Waterhouse R., Ha C., Dveksler G.S.
      J. Exp. Med. 195:277-282(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A RECEPTOR FOR PSG17, SUBCELLULAR LOCATION.
    7. "Tetraspanin protein CD9 is a novel paranodal component regulating paranodal junctional formation."
      Ishibashi T., Ding L., Ikenaka K., Inoue Y., Miyado K., Mekada E., Baba H.
      J. Neurosci. 24:96-102(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN PARANODAL FORMATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCD9_MOUSE
    AccessioniPrimary (citable) accession number: P40240
    Secondary accession number(s): Q3U9W0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3