ID   CD82_MOUSE              Reviewed;         266 AA.
AC   P40237;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   24-JAN-2024, entry version 163.
DE   RecName: Full=CD82 antigen;
DE   AltName: Full=C33 antigen;
DE   AltName: Full=IA4;
DE   AltName: Full=Inducible membrane protein R2;
DE   AltName: Full=Metastasis suppressor Kangai-1 homolog;
DE   AltName: CD_antigen=CD82;
GN   Name=Cd82; Synonyms=Kai1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=8039242; DOI=10.1006/cimm.1994.1212;
RA   Nagira M., Imai T., Ishikawa I., Uwabe K.I., Yoshie O.;
RT   "Mouse homologue of C33 antigen (CD82), a member of the transmembrane 4
RT   superfamily: complementary DNA, genomic structure, and expression.";
RL   Cell. Immunol. 157:144-157(1994).
RN   [2]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-131 AND ASN-157.
RC   TISSUE=Myoblast;
RX   PubMed=19656770; DOI=10.1074/mcp.m900195-mcp200;
RA   Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D.,
RA   Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.;
RT   "The mouse C2C12 myoblast cell surface N-linked glycoproteome:
RT   identification, glycosite occupancy, and membrane orientation.";
RL   Mol. Cell. Proteomics 8:2555-2569(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Kidney, Liver, Lung, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Associates with CD4 or CD8 and delivers costimulatory signals
CC       for the TCR/CD3 pathway.
CC   -!- SUBUNIT: Interacts directly with IGSF8. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P27701};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Highest expression in the spleen and the kidney.
CC       Low expression in skeletal muscle and in the heart.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR   EMBL; D14883; BAA03602.1; -; mRNA.
DR   CCDS; CCDS16454.1; -.
DR   PIR; I49561; I49561.
DR   RefSeq; NP_001129527.1; NM_001136055.2.
DR   RefSeq; NP_001258359.1; NM_001271430.1.
DR   RefSeq; NP_001258360.1; NM_001271431.1.
DR   RefSeq; NP_001258361.1; NM_001271432.1.
DR   RefSeq; NP_001258390.1; NM_001271461.1.
DR   RefSeq; NP_001258391.1; NM_001271462.1.
DR   RefSeq; NP_031682.1; NM_007656.5.
DR   AlphaFoldDB; P40237; -.
DR   SMR; P40237; -.
DR   BioGRID; 198614; 1.
DR   STRING; 10090.ENSMUSP00000028644; -.
DR   GlyCosmos; P40237; 4 sites, No reported glycans.
DR   GlyGen; P40237; 4 sites.
DR   iPTMnet; P40237; -.
DR   PhosphoSitePlus; P40237; -.
DR   SwissPalm; P40237; -.
DR   EPD; P40237; -.
DR   jPOST; P40237; -.
DR   PaxDb; 10090-ENSMUSP00000028644; -.
DR   PeptideAtlas; P40237; -.
DR   ProteomicsDB; 283753; -.
DR   Pumba; P40237; -.
DR   Antibodypedia; 3743; 684 antibodies from 43 providers.
DR   DNASU; 12521; -.
DR   Ensembl; ENSMUST00000028644.11; ENSMUSP00000028644.5; ENSMUSG00000027215.14.
DR   Ensembl; ENSMUST00000099696.8; ENSMUSP00000097287.2; ENSMUSG00000027215.14.
DR   Ensembl; ENSMUST00000111257.8; ENSMUSP00000106888.2; ENSMUSG00000027215.14.
DR   Ensembl; ENSMUST00000116457.9; ENSMUSP00000112158.3; ENSMUSG00000027215.14.
DR   GeneID; 12521; -.
DR   KEGG; mmu:12521; -.
DR   UCSC; uc008lfx.2; mouse.
DR   AGR; MGI:104651; -.
DR   CTD; 3732; -.
DR   MGI; MGI:104651; Cd82.
DR   VEuPathDB; HostDB:ENSMUSG00000027215; -.
DR   eggNOG; KOG3882; Eukaryota.
DR   GeneTree; ENSGT00940000158481; -.
DR   InParanoid; P40237; -.
DR   OMA; TTEHAWD; -.
DR   OrthoDB; 4585533at2759; -.
DR   PhylomeDB; P40237; -.
DR   TreeFam; TF352892; -.
DR   BioGRID-ORCS; 12521; 2 hits in 77 CRISPR screens.
DR   ChiTaRS; Cd82; mouse.
DR   PRO; PR:P40237; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; P40237; Protein.
DR   Bgee; ENSMUSG00000027215; Expressed in parotid gland and 253 other cell types or tissues.
DR   ExpressionAtlas; P40237; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   CDD; cd03160; CD37_CD82_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   PANTHER; PTHR19282:SF44; CD82 ANTIGEN; 1.
DR   PANTHER; PTHR19282; TETRASPANIN; 1.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   PROSITE; PS00421; TM4_1; 1.
DR   Genevisible; P40237; MM.
PE   1: Evidence at protein level;
KW   Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..266
FT                   /note="CD82 antigen"
FT                   /id="PRO_0000219227"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..53
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        73..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        111..227
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        228..249
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        250..266
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        127
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        131
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19656770"
FT   CARBOHYD        157
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19656770"
FT   CARBOHYD        197
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   266 AA;  29629 MW;  CB4BE669E7C52D57 CRC64;
     MGAGCVKVTK YFLFLFNLLF FILGAVILGF GVWILADKNS FISVLQTSSS SLQVGAYVFI
     GVGAITIVMG FLGCIGAVNE VRCLLGLYFV FLLLILIAQV TVGVLFYFNA DKLKKEMGNT
     VMDIIRNYTA NATSSREEAW DYVQAQVKCC GWVSHYNWTE NEELMGFTKT TYPCSCEKIK
     EEDNQLIVKK GFCEADNSTV SENNPEDWPV NTEGCMEKAQ AWLQENFGIL LGVCAGVAVI
     ELLGLFLSIC LCRYIHSEDY SKVPKY
//