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Protein

Casein kinase I homolog 1

Gene

cki1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP
Active sitei131 – 1311Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATP

GO - Molecular functioni

  • ATP binding Source: PomBase
  • magnesium ion binding Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase
  • protein tyrosine kinase activity Source: PomBase

GO - Biological processi

  • peptidyl-tyrosine autophosphorylation Source: PomBase
  • protein phosphorylation Source: PomBase
  • regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: PomBase
  • regulation of endocytosis Source: PomBase
  • signal transduction Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I homolog 1 (EC:2.7.11.1)
Gene namesi
Name:cki1
ORF Names:SPBC1347.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1347.06c.
PomBaseiSPBC1347.06c. cki1.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: PomBase
  • vacuole Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Casein kinase I homolog 1PRO_0000192861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei329 – 3291Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40233.

PTM databases

iPTMnetiP40233.

Interactioni

Protein-protein interaction databases

BioGridi276168. 2 interactions.
IntActiP40233. 1 interaction.
MINTiMINT-4689462.

Structurei

Secondary structure

1
446
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni9 – 113Combined sources
Beta strandi12 – 209Combined sources
Beta strandi25 – 317Combined sources
Turni32 – 354Combined sources
Beta strandi36 – 449Combined sources
Helixi52 – 6110Combined sources
Turni62 – 643Combined sources
Beta strandi65 – 673Combined sources
Beta strandi71 – 777Combined sources
Beta strandi80 – 867Combined sources
Helixi92 – 987Combined sources
Turni99 – 1013Combined sources
Helixi105 – 12319Combined sources
Turni124 – 1263Combined sources
Helixi134 – 1363Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi142 – 1443Combined sources
Turni145 – 1484Combined sources
Beta strandi150 – 1523Combined sources
Beta strandi159 – 1624Combined sources
Turni164 – 1663Combined sources
Turni182 – 1843Combined sources
Helixi187 – 1904Combined sources
Helixi197 – 21317Combined sources
Helixi226 – 23914Combined sources
Helixi242 – 2454Combined sources
Turni246 – 2483Combined sources
Helixi251 – 26212Combined sources
Helixi271 – 28414Combined sources
Helixi294 – 2963Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CSNX-ray2.00A1-298[»]
1EH4X-ray2.80A/B1-298[»]
2CSNX-ray2.50A2-298[»]
ProteinModelPortaliP40233.
SMRiP40233. Positions 6-298.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40233.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 274263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000182055.
InParanoidiP40233.
KOiK02218.
OMAiPRKCEAP.
OrthoDBiEOG7WQ832.
PhylomeDBiP40233.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQNNVVGV HYKVGRRIGE GSFGVIFEGT NLLNNQQVAI KFEPRRSDAP
60 70 80 90 100
QLRDEYRTYK LLAGCTGIPN VYYFGQEGLH NILVIDLLGP SLEDLLDLCG
110 120 130 140 150
RKFSVKTVAM AAKQMLARVQ SIHEKSLVYR DIKPDNFLIG RPNSKNANMI
160 170 180 190 200
YVVDFGMVKF YRDPVTKQHI PYREKKNLSG TARYMSINTH LGREQSRRDD
210 220 230 240 250
LEALGHVFMY FLRGSLPWQG LKAATNKQKY ERIGEKKQST PLRELCAGFP
260 270 280 290 300
EEFYKYMHYA RNLAFDATPD YDYLQGLFSK VLERLNTTED ENFDWNLLNN
310 320 330 340 350
GKGWQSLKSR NAETENQRSS KPPAPKLESK SPALQNHAST QNVVSKRSDY
360 370 380 390 400
EKPFAEPHLN SASDSAEPNQ NSLPNPPTET KATTTVPDRS GLATNQPAPV
410 420 430 440
DVHDSSEERV TREQVQNATK ETEAPKKKKS FWASILSCCS GSNEDT
Length:446
Mass (Da):50,425
Last modified:December 1, 2000 - v2
Checksum:iD813483056CD8FD8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821I → V in AAA19019 (PubMed:8163505).Curated
Sequence conflicti310 – 3101R → Q in AAA19019 (PubMed:8163505).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06929 mRNA. Translation: AAA19019.1.
CU329671 Genomic DNA. Translation: CAB37437.1.
PIRiA53581.
RefSeqiNP_596698.1. NM_001022622.2.

Genome annotation databases

EnsemblFungiiSPBC1347.06c.1; SPBC1347.06c.1:pep; SPBC1347.06c.
GeneIDi2539610.
KEGGispo:SPBC1347.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06929 mRNA. Translation: AAA19019.1.
CU329671 Genomic DNA. Translation: CAB37437.1.
PIRiA53581.
RefSeqiNP_596698.1. NM_001022622.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CSNX-ray2.00A1-298[»]
1EH4X-ray2.80A/B1-298[»]
2CSNX-ray2.50A2-298[»]
ProteinModelPortaliP40233.
SMRiP40233. Positions 6-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276168. 2 interactions.
IntActiP40233. 1 interaction.
MINTiMINT-4689462.

PTM databases

iPTMnetiP40233.

Proteomic databases

MaxQBiP40233.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1347.06c.1; SPBC1347.06c.1:pep; SPBC1347.06c.
GeneIDi2539610.
KEGGispo:SPBC1347.06c.

Organism-specific databases

EuPathDBiFungiDB:SPBC1347.06c.
PomBaseiSPBC1347.06c. cki1.

Phylogenomic databases

HOGENOMiHOG000182055.
InParanoidiP40233.
KOiK02218.
OMAiPRKCEAP.
OrthoDBiEOG7WQ832.
PhylomeDBiP40233.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.

Miscellaneous databases

EvolutionaryTraceiP40233.
NextBioi20800767.
PROiP40233.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cytoplasmic forms of fission yeast casein kinase-1 associate primarily with the particulate fraction of the cell."
    Wang P.-C., Vancura A., Desai A., Carmel G., Kuret J.
    J. Biol. Chem. 269:12014-12023(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SP66.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Crystal structure of casein kinase-1, a phosphate-directed protein kinase."
    Xu R.-M., Carmel G., Sweet R.M., Kuret J., Cheng X.
    EMBO J. 14:1015-1023(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-298.

Entry informationi

Entry nameiCKI1_SCHPO
AccessioniPrimary (citable) accession number: P40233
Secondary accession number(s): Q9UUL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 1, 2000
Last modified: May 11, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.