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P40226 (TPO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thrombopoietin
Alternative name(s):
C-mpl ligand
Short name=ML
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
Short name=MGDF
Myeloproliferative leukemia virus oncogene ligand
Gene names
Name:Thpo
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Subcellular location

Secreted.

Tissue specificity

Found mainly in the liver, kidney and skeletal muscle.

Induction

Expression in the liver and bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle). Ref.5

Domain

Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

Sequence similarities

Belongs to the EPO/TPO family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P40226-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P40226-2)

Also known as: TPO-2;

The sequence of this isoform differs from the canonical sequence as follows:
     133-136: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 356335Thrombopoietin
PRO_0000008412

Amino acid modifications

Glycosylation1971N-linked (GlcNAc...) Potential
Glycosylation2061N-linked (GlcNAc...) Potential
Glycosylation2351N-linked (GlcNAc...) Potential
Glycosylation2491N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 172 Potential
Disulfide bond50 ↔ 106 Potential

Natural variations

Alternative sequence133 – 1364Missing in isoform 2.
VSP_001452

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: D346DBAE8392053C

FASTA35637,836
        10         20         30         40         50         60 
MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC PDVDPLSIPV 

        70         80         90        100        110        120 
LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL 

       130        140        150        160        170        180 
LLGALQGLLG TQLPLQGRTT AHKDPNALFL SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT 

       190        200        210        220        230        240 
AVPSSTSQLL TLNKFPNRTS GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS 

       250        260        270        280        290        300 
PVQISGYLNR THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA 

       310        320        330        340        350 
PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR NLSQET 

« Hide

Isoform 2 (TPO-2) [UniParc].

Checksum: B7121C4BC7CCAF4A
Show »

FASTA35237,384

References

« Hide 'large scale' references
[1]"Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo."
Lok S., Kaushansky K., Holly R.D., Kuijper J.L., Lofton-Day C.E., Oort P.J., Grant F.J., Heipel M.D., Burkhead S.K., Kramer J.M., Bell L.A.N., Sprecher C.A., Blumberg H., Johnson R., Prunkard D., Ching A.F.T., Mathewes S.L., Bailey M.C. expand/collapse author list , Forstrom J.W., Buddle M.M., Osborn S.G., Evans S.J., Sheppard P.O., Presnell S.R., O'Hara P.J., Hagen F.S., Roth G.J., Foster D.C.
Nature 369:565-568(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Kidney.
[2]"Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl."
Bartley T.D., Bogenberger J., Hunt P., Li Y.-S., Lu H.S., Martin F., Chang M.-S., Samal B.B., Nichol J.L., Swift S., Johnson M.J., Hsu R.-Y., Parker V.P., Suggs S., Skrine J.D., Merewether L.A., Clogson C., Hsu E. expand/collapse author list , Hokom M.M., Hornkohl A., Choi E., Pangelinan M., Sun Y., Mar V., McNich J., Simonet L., Jacobsen F., Xie C., Shutter J., Chute H., Basu R., Selander L., Trollinger D., Sieu L., Padilla D., Trail G., Elliott G., Izumi R., Covey T., Crouse J., Garcia A., Xu W., del Castillo J., Biron J., Cole S., Hu M.C.-T., Pacifici R., Ponting I., Saris C., Wen D., Yung Y.P., Lin H., Bosselman R.A.
Cell 77:1117-1124(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[3]"Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin."
Gurney A.L., Kuang W.-J., Xie M.-H., Malloy B.E., Eaton D.L., de Sauvage F.J.
Blood 85:981-988(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Diurnal expression of the thrombopoietin gene is regulated by CLOCK."
Tracey C.J., Pan X., Catterson J.H., Harmar A.J., Hussain M.M., Hartley P.S.
J. Thromb. Haemost. 10:662-669(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L34169 mRNA. Translation: AAA40436.1.
BC003803 mRNA. Translation: AAH03803.1.
PIRS45330.
RefSeqNP_001166976.1. NM_001173505.1.
NP_033405.1. NM_009379.3.
UniGeneMm.3943.

3D structure databases

ProteinModelPortalP40226.
SMRP40226. Positions 28-172.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000075756.

PTM databases

PhosphoSiteP40226.

Proteomic databases

PRIDEP40226.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847. [P40226-1]
ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847. [P40226-1]
GeneID21832.
KEGGmmu:21832.
UCSCuc007yra.2. mouse. [P40226-1]

Organism-specific databases

CTD7066.
MGIMGI:101875. Thpo.

Phylogenomic databases

eggNOGNOG84342.
HOGENOMHOG000082495.
HOVERGENHBG002468.
InParanoidP40226.
KOK06854.
OMAEQSKAQD.
OrthoDBEOG7PVWPW.
PhylomeDBP40226.
TreeFamTF338084.

Gene expression databases

BgeeP40226.
CleanExMM_THPO.
GenevestigatorP40226.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR019767. EPO/TPO_CS.
IPR001323. EPO_TPO.
IPR003978. Thrombopoeitin.
[Graphical view]
PANTHERPTHR10560. PTHR10560. 1 hit.
PfamPF00758. EPO_TPO. 1 hit.
[Graphical view]
PRINTSPR01485. THROMBOPTN.
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00817. EPO_TPO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301278.
PROP40226.
SOURCESearch...

Entry information

Entry nameTPO_MOUSE
AccessionPrimary (citable) accession number: P40226
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot