Reviewed, UniProtKB/Swiss-Prot P40226 (TPO_MOUSE)

Names and origin

Protein names

Recommended name:
    Thrombopoietin
Alternative name(s):
    Megakaryocyte colony-stimulating factor
    Myeloproliferative leukemia virus oncogene ligand
    C-mpl ligand
      Short name=ML
    Megakaryocyte growth and development factor
      Short name=MGDF

Gene names

Name:

Thpo

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Subcellular location	Secreted.
Tissue specificity	Found mainly in the liver, kidney and skeletal muscle.
Domain	Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
Sequence similarities	Belongs to the EPO/TPO family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Cytokine Hormone
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell proliferation Inferred from electronic annotation. Source: InterPro myeloid cell differentiation Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P40226-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P40226-2)

Also known as: TPO-2;

The sequence of this isoform differs from the canonical sequence as follows:
     133-136: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 356	335	Thrombopoietin		PRO_0000008412
Amino acid modifications
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
	Glycosylation	235	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Glycosylation	256	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	351	1	N-linked (GlcNAc...) Potential
	Disulfide bond	28 ↔ 172		Potential
	Disulfide bond	50 ↔ 106		Potential
Natural variations
	Alternative sequence	133 – 136	4	Missing in isoform 2.		VSP_001452

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D346DBAE8392053C	FASTA	356	37,836
	10 20 30 40 50 60 MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC PDVDPLSIPV 70 80 90 100 110 120 LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL 130 140 150 160 170 180 LLGALQGLLG TQLPLQGRTT AHKDPNALFL SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT 190 200 210 220 230 240 AVPSSTSQLL TLNKFPNRTS GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS 250 260 270 280 290 300 PVQISGYLNR THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA 310 320 330 340 350 PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR NLSQET
	Isoform 2 (TPO-2). Checksum: B7121C4BC7CCAF4A	FASTA	352	37,384
	10 20 30 40 50 60 MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC PDVDPLSIPV 70 80 90 100 110 120 LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG QLEPSCLSSL LGQLSGQVRL 130 140 150 160 170 180 LLGALQGLLG TQGRTTAHKD PNALFLSLQQ LLRGKVRFLL LVEGPTLCVR RTLPTTAVPS 190 200 210 220 230 240 STSQLLTLNK FPNRTSGLLE TNFSVTARTA GPGLLSRLQG FRVKITPGQL NQTSRSPVQI 250 260 270 280 290 300 SGYLNRTHGP VNGTHGLFAG TSLQTLEASD ISPGAFNKGS LAFNLQGGLP PSPSLAPDGH 310 320 330 340 350 TPFPPSPALP TTHGSPPQLH PLFPDPSTTM PNSTAPHPVT MYPHPRNLSQ ET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo." Lok S., Kaushansky K., Holly R.D., Kuijper J.L., Lofton-Day C.E., Oort P.J., Grant F.J., Heipel M.D., Burkhead S.K., Kramer J.M., Bell L.A.N., Sprecher C.A., Blumberg H., Johnson R., Prunkard D., Ching A.F.T., Mathewes S.L., Bailey M.C. , Forstrom J.W., Buddle M.M., Osborn S.G., Evans S.J., Sheppard P.O., Presnell S.R., O'Hara P.J., Hagen F.S., Roth G.J., Foster D.C. Nature 369:565-568(1994) [PubMed: 8202158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Kidney.
[2]	"Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl." Bartley T.D., Bogenberger J., Hunt P., Li Y.-S., Lu H.S., Martin F., Chang M.-S., Samal B.B., Nichol J.L., Swift S., Johnson M.J., Hsu R.-Y., Parker V.P., Suggs S., Skrine J.D., Merewether L.A., Clogson C., Hsu E. , Hokom M.M., Hornkohl A., Choi E., Pangelinan M., Sun Y., Mar V., McNich J., Simonet L., Jacobsen F., Xie C., Shutter J., Chute H., Basu R., Selander L., Trollinger D., Sieu L., Padilla D., Trail G., Elliott G., Izumi R., Covey T., Crouse J., Garcia A., Xu W., del Castillo J., Biron J., Cole S., Hu M.C.-T., Pacifici R., Ponting I., Saris C., Wen D., Yung Y.P., Lin H., Bosselman R.A. Cell 77:1117-1124(1994) [PubMed: 8020099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[3]	"Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin." Gurney A.L., Kuang W.-J., Xie M.-H., Malloy B.E., Eaton D.L., de Sauvage F.J. Blood 85:981-988(1995) [PubMed: 7849319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L34169 mRNA. Translation: AAA40436.1. BC003803 mRNA. Translation: AAH03803.1.
IPI	IPI00108063. IPI00230271.
PIR	S45330.
RefSeq	NP_033405.1.
UniGene	Mm.3943
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P40226.
Proteomic databases
PRIDE	P40226.
Genome annotation databases
Ensembl	ENSMUSG00000022847. Mus musculus. [Contig view]
GeneID	21832.
KEGG	mmu:21832.
Organism-specific databases
MGI	MGI:101875. Thpo.
Phylogenomic databases
HOGENOM	P40226.
HOVERGEN	P40226.
OMA	P40226. QGRTTAH.
Gene expression databases
ArrayExpress	P40226.
Bgee	P40226.
CleanEx	MM_THPO.
GermOnline	ENSMUSG00000022847. Mus musculus.
Family and domain databases
InterPro	IPR012351. 4_helix_cytokine_core. IPR019767. EPO/TPO_CS. IPR001323. EPO_TPO. IPR003978. Thrombopoeitin. [Graphical view]
Gene3D	G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.
Pfam	PF00758. EPO_TPO. 1 hit. [Graphical view]
PRINTS	PR01485. THROMBOPTN.
PROSITE	PS00817. EPO_TPO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301278.
SOURCE	Search...

Entry information

Entry name

TPO_MOUSE

Accession

Primary (citable) accession number: P40226

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)