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P40226

- TPO_MOUSE

UniProt

P40226 - TPO_MOUSE

Protein

Thrombopoietin

Gene

Thpo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

    GO - Biological processi

    1. cell proliferation Source: InterPro
    2. myeloid cell differentiation Source: MGI
    3. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
    4. positive regulation of megakaryocyte differentiation Source: UniProtKB
    5. positive regulation of protein kinase B signaling Source: UniProtKB
    6. positive regulation of protein phosphorylation Source: UniProtKB
    7. thrombopoietin-mediated signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Cytokine, Hormone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombopoietin
    Alternative name(s):
    C-mpl ligand
    Short name:
    ML
    Megakaryocyte colony-stimulating factor
    Megakaryocyte growth and development factor
    Short name:
    MGDF
    Myeloproliferative leukemia virus oncogene ligand
    Gene namesi
    Name:Thpo
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:101875. Thpo.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 356335ThrombopoietinPRO_0000008412Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 172Sequence Analysis
    Disulfide bondi50 ↔ 106Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP40226.

    PTM databases

    PhosphoSiteiP40226.

    Expressioni

    Tissue specificityi

    Found mainly in the liver, kidney and skeletal muscle.

    Inductioni

    Expression in the liver and bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle).1 Publication

    Gene expression databases

    BgeeiP40226.
    CleanExiMM_THPO.
    GenevestigatoriP40226.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000075756.

    Structurei

    3D structure databases

    ProteinModelPortaliP40226.
    SMRiP40226. Positions 28-172.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

    Sequence similaritiesi

    Belongs to the EPO/TPO family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG84342.
    HOGENOMiHOG000082495.
    HOVERGENiHBG002468.
    InParanoidiP40226.
    KOiK06854.
    OMAiEQSKAQD.
    OrthoDBiEOG7PVWPW.
    PhylomeDBiP40226.
    TreeFamiTF338084.

    Family and domain databases

    Gene3Di1.20.1250.10. 1 hit.
    InterProiIPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR019767. EPO/TPO_CS.
    IPR001323. EPO_TPO.
    IPR003978. Thrombopoeitin.
    [Graphical view]
    PANTHERiPTHR10560. PTHR10560. 1 hit.
    PfamiPF00758. EPO_TPO. 1 hit.
    [Graphical view]
    PRINTSiPR01485. THROMBOPTN.
    SUPFAMiSSF47266. SSF47266. 1 hit.
    PROSITEiPS00817. EPO_TPO. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P40226-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC    50
    PDVDPLSIPV LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG 100
    QLEPSCLSSL LGQLSGQVRL LLGALQGLLG TQLPLQGRTT AHKDPNALFL 150
    SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT AVPSSTSQLL TLNKFPNRTS 200
    GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS PVQISGYLNR 250
    THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA 300
    PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR 350
    NLSQET 356
    Length:356
    Mass (Da):37,836
    Last modified:February 1, 1995 - v1
    Checksum:iD346DBAE8392053C
    GO
    Isoform 2 (identifier: P40226-2) [UniParc]FASTAAdd to Basket

    Also known as: TPO-2

    The sequence of this isoform differs from the canonical sequence as follows:
         133-136: Missing.

    Show »
    Length:352
    Mass (Da):37,384
    Checksum:iB7121C4BC7CCAF4A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei133 – 1364Missing in isoform 2. 1 PublicationVSP_001452

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34169 mRNA. Translation: AAA40436.1.
    BC003803 mRNA. Translation: AAH03803.1.
    CCDSiCCDS37292.1. [P40226-1]
    PIRiS45330.
    RefSeqiNP_001166976.1. NM_001173505.1. [P40226-1]
    NP_001276823.1. NM_001289894.1. [P40226-2]
    NP_033405.1. NM_009379.3. [P40226-1]
    UniGeneiMm.3943.

    Genome annotation databases

    EnsembliENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847. [P40226-1]
    ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847. [P40226-1]
    GeneIDi21832.
    KEGGimmu:21832.
    UCSCiuc007yra.2. mouse. [P40226-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34169 mRNA. Translation: AAA40436.1 .
    BC003803 mRNA. Translation: AAH03803.1 .
    CCDSi CCDS37292.1. [P40226-1 ]
    PIRi S45330.
    RefSeqi NP_001166976.1. NM_001173505.1. [P40226-1 ]
    NP_001276823.1. NM_001289894.1. [P40226-2 ]
    NP_033405.1. NM_009379.3. [P40226-1 ]
    UniGenei Mm.3943.

    3D structure databases

    ProteinModelPortali P40226.
    SMRi P40226. Positions 28-172.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000075756.

    PTM databases

    PhosphoSitei P40226.

    Proteomic databases

    PRIDEi P40226.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000076422 ; ENSMUSP00000075756 ; ENSMUSG00000022847 . [P40226-1 ]
    ENSMUST00000115437 ; ENSMUSP00000111097 ; ENSMUSG00000022847 . [P40226-1 ]
    GeneIDi 21832.
    KEGGi mmu:21832.
    UCSCi uc007yra.2. mouse. [P40226-1 ]

    Organism-specific databases

    CTDi 7066.
    MGIi MGI:101875. Thpo.

    Phylogenomic databases

    eggNOGi NOG84342.
    HOGENOMi HOG000082495.
    HOVERGENi HBG002468.
    InParanoidi P40226.
    KOi K06854.
    OMAi EQSKAQD.
    OrthoDBi EOG7PVWPW.
    PhylomeDBi P40226.
    TreeFami TF338084.

    Miscellaneous databases

    NextBioi 301278.
    PROi P40226.
    SOURCEi Search...

    Gene expression databases

    Bgeei P40226.
    CleanExi MM_THPO.
    Genevestigatori P40226.

    Family and domain databases

    Gene3Di 1.20.1250.10. 1 hit.
    InterProi IPR009079. 4_helix_cytokine-like_core.
    IPR012351. 4_helix_cytokine_core.
    IPR019767. EPO/TPO_CS.
    IPR001323. EPO_TPO.
    IPR003978. Thrombopoeitin.
    [Graphical view ]
    PANTHERi PTHR10560. PTHR10560. 1 hit.
    Pfami PF00758. EPO_TPO. 1 hit.
    [Graphical view ]
    PRINTSi PR01485. THROMBOPTN.
    SUPFAMi SSF47266. SSF47266. 1 hit.
    PROSITEi PS00817. EPO_TPO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Kidney.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Liver.
    3. "Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin."
      Gurney A.L., Kuang W.-J., Xie M.-H., Malloy B.E., Eaton D.L., de Sauvage F.J.
      Blood 85:981-988(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Diurnal expression of the thrombopoietin gene is regulated by CLOCK."
      Tracey C.J., Pan X., Catterson J.H., Harmar A.J., Hussain M.M., Hartley P.S.
      J. Thromb. Haemost. 10:662-669(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiTPO_MOUSE
    AccessioniPrimary (citable) accession number: P40226
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3