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P40226

- TPO_MOUSE

UniProt

P40226 - TPO_MOUSE

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Protein

Thrombopoietin

Gene

Thpo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

GO - Biological processi

  1. cell proliferation Source: InterPro
  2. myeloid cell differentiation Source: MGI
  3. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  4. positive regulation of megakaryocyte differentiation Source: UniProtKB
  5. positive regulation of protein kinase B signaling Source: UniProtKB
  6. positive regulation of protein phosphorylation Source: UniProtKB
  7. thrombopoietin-mediated signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Hormone

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombopoietin
Alternative name(s):
C-mpl ligand
Short name:
ML
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
Short name:
MGDF
Myeloproliferative leukemia virus oncogene ligand
Gene namesi
Name:Thpo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:101875. Thpo.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 356335ThrombopoietinPRO_0000008412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 172Sequence Analysis
Disulfide bondi50 ↔ 106Sequence Analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP40226.

PTM databases

PhosphoSiteiP40226.

Expressioni

Tissue specificityi

Found mainly in the liver, kidney and skeletal muscle.

Inductioni

Expression in the liver and bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle).1 Publication

Gene expression databases

BgeeiP40226.
CleanExiMM_THPO.
ExpressionAtlasiP40226. baseline.
GenevestigatoriP40226.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075756.

Structurei

3D structure databases

ProteinModelPortaliP40226.
SMRiP40226. Positions 28-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

Sequence similaritiesi

Belongs to the EPO/TPO family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG84342.
HOGENOMiHOG000082495.
HOVERGENiHBG002468.
InParanoidiP40226.
KOiK06854.
OMAiEQSKAQD.
OrthoDBiEOG7PVWPW.
PhylomeDBiP40226.
TreeFamiTF338084.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR019767. EPO/TPO_CS.
IPR001323. EPO_TPO.
IPR003978. Thrombopoeitin.
[Graphical view]
PANTHERiPTHR10560. PTHR10560. 1 hit.
PfamiPF00758. EPO_TPO. 1 hit.
[Graphical view]
PRINTSiPR01485. THROMBOPTN.
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00817. EPO_TPO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P40226-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC
60 70 80 90 100
PDVDPLSIPV LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG
110 120 130 140 150
QLEPSCLSSL LGQLSGQVRL LLGALQGLLG TQLPLQGRTT AHKDPNALFL
160 170 180 190 200
SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT AVPSSTSQLL TLNKFPNRTS
210 220 230 240 250
GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS PVQISGYLNR
260 270 280 290 300
THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA
310 320 330 340 350
PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR

NLSQET
Length:356
Mass (Da):37,836
Last modified:February 1, 1995 - v1
Checksum:iD346DBAE8392053C
GO
Isoform 2 (identifier: P40226-2) [UniParc]FASTAAdd to Basket

Also known as: TPO-2

The sequence of this isoform differs from the canonical sequence as follows:
     133-136: Missing.

Show »
Length:352
Mass (Da):37,384
Checksum:iB7121C4BC7CCAF4A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei133 – 1364Missing in isoform 2. 1 PublicationVSP_001452

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34169 mRNA. Translation: AAA40436.1.
BC003803 mRNA. Translation: AAH03803.1.
CCDSiCCDS37292.1. [P40226-1]
PIRiS45330.
RefSeqiNP_001166976.1. NM_001173505.1. [P40226-1]
NP_001276823.1. NM_001289894.1. [P40226-2]
NP_033405.1. NM_009379.3. [P40226-1]
UniGeneiMm.3943.

Genome annotation databases

EnsembliENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847. [P40226-1]
ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847. [P40226-1]
GeneIDi21832.
KEGGimmu:21832.
UCSCiuc007yra.2. mouse. [P40226-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34169 mRNA. Translation: AAA40436.1 .
BC003803 mRNA. Translation: AAH03803.1 .
CCDSi CCDS37292.1. [P40226-1 ]
PIRi S45330.
RefSeqi NP_001166976.1. NM_001173505.1. [P40226-1 ]
NP_001276823.1. NM_001289894.1. [P40226-2 ]
NP_033405.1. NM_009379.3. [P40226-1 ]
UniGenei Mm.3943.

3D structure databases

ProteinModelPortali P40226.
SMRi P40226. Positions 28-172.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000075756.

PTM databases

PhosphoSitei P40226.

Proteomic databases

PRIDEi P40226.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000076422 ; ENSMUSP00000075756 ; ENSMUSG00000022847 . [P40226-1 ]
ENSMUST00000115437 ; ENSMUSP00000111097 ; ENSMUSG00000022847 . [P40226-1 ]
GeneIDi 21832.
KEGGi mmu:21832.
UCSCi uc007yra.2. mouse. [P40226-1 ]

Organism-specific databases

CTDi 7066.
MGIi MGI:101875. Thpo.

Phylogenomic databases

eggNOGi NOG84342.
HOGENOMi HOG000082495.
HOVERGENi HBG002468.
InParanoidi P40226.
KOi K06854.
OMAi EQSKAQD.
OrthoDBi EOG7PVWPW.
PhylomeDBi P40226.
TreeFami TF338084.

Miscellaneous databases

NextBioi 301278.
PROi P40226.
SOURCEi Search...

Gene expression databases

Bgeei P40226.
CleanExi MM_THPO.
ExpressionAtlasi P40226. baseline.
Genevestigatori P40226.

Family and domain databases

Gene3Di 1.20.1250.10. 1 hit.
InterProi IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR019767. EPO/TPO_CS.
IPR001323. EPO_TPO.
IPR003978. Thrombopoeitin.
[Graphical view ]
PANTHERi PTHR10560. PTHR10560. 1 hit.
Pfami PF00758. EPO_TPO. 1 hit.
[Graphical view ]
PRINTSi PR01485. THROMBOPTN.
SUPFAMi SSF47266. SSF47266. 1 hit.
PROSITEi PS00817. EPO_TPO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin."
    Gurney A.L., Kuang W.-J., Xie M.-H., Malloy B.E., Eaton D.L., de Sauvage F.J.
    Blood 85:981-988(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Diurnal expression of the thrombopoietin gene is regulated by CLOCK."
    Tracey C.J., Pan X., Catterson J.H., Harmar A.J., Hussain M.M., Hartley P.S.
    J. Thromb. Haemost. 10:662-669(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiTPO_MOUSE
AccessioniPrimary (citable) accession number: P40226
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3