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P40223

- CSF3R_MOUSE

UniProt

P40223 - CSF3R_MOUSE

Protein

Granulocyte colony-stimulating factor receptor

Gene

Csf3r

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for granulocyte colony-stimulating factor (CSF3). In addition it may function in some adhesion or recognition events at the cell surface.

    GO - Molecular functioni

    1. cytokine receptor activity Source: InterPro

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. neutrophil chemotaxis Source: MGI
    3. odontogenesis of dentin-containing tooth Source: Ensembl

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granulocyte colony-stimulating factor receptor
    Short name:
    G-CSF receptor
    Short name:
    G-CSF-R
    Alternative name(s):
    CD_antigen: CD114
    Gene namesi
    Name:Csf3r
    Synonyms:Csfgr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1339755. Csf3r.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 837812Granulocyte colony-stimulating factor receptorPRO_0000010875Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 52By similarity
    Disulfide bondi46 ↔ 102By similarity
    Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi94 – 941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi129 – 1291N-linked (GlcNAc...)1 Publication
    Disulfide bondi132 ↔ 1431 Publication
    Disulfide bondi168 ↔ 2191 Publication
    Disulfide bondi178 ↔ 1871 Publication
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi249 ↔ 2961 Publication
    Disulfide bondi267 ↔ 3101 Publication
    Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi474 – 4741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi582 – 5821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi613 – 6131N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP40223.

    PTM databases

    PhosphoSiteiP40223.

    Expressioni

    Tissue specificityi

    Found in bone marrow.

    Gene expression databases

    BgeeiP40223.
    GenevestigatoriP40223.

    Interactioni

    Subunit structurei

    Homodimer. The dimeric receptor binds two CSF3 molecules.1 Publication

    Protein-protein interaction databases

    BioGridi198935. 3 interactions.
    STRINGi10090.ENSMUSP00000101768.

    Structurei

    Secondary structure

    1
    837
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi128 – 1358
    Turni136 – 1394
    Beta strandi140 – 1467
    Beta strandi156 – 1638
    Helixi166 – 1683
    Beta strandi174 – 1796
    Beta strandi186 – 1905
    Helixi191 – 1933
    Beta strandi200 – 2089
    Beta strandi211 – 2144
    Beta strandi218 – 2203
    Helixi222 – 2254
    Beta strandi232 – 2354
    Beta strandi242 – 2443
    Beta strandi250 – 2556
    Helixi258 – 2603
    Beta strandi265 – 27511
    Beta strandi281 – 2888
    Beta strandi290 – 2956
    Beta strandi304 – 31512
    Beta strandi326 – 3283

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CD9X-ray2.80B/D120-334[»]
    1CTONMR-A228-333[»]
    1GCFNMR-A228-333[»]
    1PGRX-ray3.50B/D/F/H120-334[»]
    ProteinModelPortaliP40223.
    SMRiP40223. Positions 26-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40223.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 626601ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini651 – 837187CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei627 – 65024HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 11893Ig-like C2-typeAdd
    BLAST
    Domaini126 – 231106Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini236 – 33196Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini334 – 433100Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini434 – 52996Fibronectin type-III 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini530 – 62495Fibronectin type-III 5PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi319 – 3235WSXWS motif
    Motifi658 – 6669Box 1 motif

    Domaini

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

    Sequence similaritiesi

    Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG29566.
    GeneTreeiENSGT00550000074436.
    HOGENOMiHOG000231142.
    HOVERGENiHBG051130.
    InParanoidiA2A8Y3.
    KOiK05061.
    OMAiYLRCDST.
    OrthoDBiEOG7FXZXN.
    TreeFamiTF338122.

    Family and domain databases

    Gene3Di2.60.40.10. 5 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR003529. Hematopoietin_rcpt_Gp130_CS.
    IPR013783. Ig-like_fold.
    IPR010457. IgC2-like_lig-bd.
    [Graphical view]
    PfamiPF00041. fn3. 1 hit.
    PF06328. Lep_receptor_Ig. 1 hit.
    [Graphical view]
    SMARTiSM00060. FN3. 4 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 4 hits.
    PROSITEiPS50853. FN3. 5 hits.
    PS01353. HEMATOPO_REC_L_F2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40223-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVGLGACTLT GVTLIFLLLP RSLESCGHIE ISPPVVRLGD PVLASCTISP    50
    NCSKLDQQAK ILWRLQDEPI QPGDRQHHLP DGTQESLITL PHLNYTQAFL 100
    FCLVPWEDSV QLLDQAELHA GYPPASPSNL SCLMHLTTNS LVCQWEPGPE 150
    THLPTSFILK SFRSRADCQY QGDTIPDCVA KKRQNNCSIP RKNLLLYQYM 200
    AIWVQAENML GSSESPKLCL DPMDVVKLEP PMLQALDIGP DVVSHQPGCL 250
    WLSWKPWKPS EYMEQECELR YQPQLKGANW TLVFHLPSSK DQFELCGLHQ 300
    APVYTLQMRC IRSSLPGFWS PWSPGLQLRP TMKAPTIRLD TWCQKKQLDP 350
    GTVSVQLFWK PTPLQEDSGQ IQGYLLSWSS PDHQGQDIHL CNTTQLSCIF 400
    LLPSEAQNVT LVAYNKAGTS SPTTVVFLEN EGPAVTGLHA MAQDLNTIWV 450
    DWEAPSLLPQ GYLIEWEMSS PSYNNSYKSW MIEPNGNITG ILLKDNINPF 500
    QLYRITVAPL YPGIVGPPVN VYTFAGERAP PHAPALHLKH VGTTWAQLEW 550
    VPEAPRLGMI PLTHYTIFWA DAGDHSFSVT LNISLHDFVL KHLEPASLYH 600
    VYLMATSRAG STNSTGLTLR TLDPSDLNIF LGILCLVLLS TTCVVTWLCC 650
    KRRGKTSFWS DVPDPAHSSL SSWLPTIMTE ETFQLPSFWD SSVPSITKIT 700
    ELEEDKKPTH WDSESSGNGS LPALVQAYVL QGDPREISNQ SQPPSRTGDQ 750
    VLYGQVLESP TSPGVMQYIR SDSTQPLLGG PTPSPKSYEN IWFHSRPQET 800
    FVPQPPNQED DCVFGPPFDF PLFQGLQVHG VEEQGGF 837
    Length:837
    Mass (Da):93,379
    Last modified:July 27, 2011 - v2
    Checksum:iF46E9D62A3DC4C3F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti379 – 3791S → N in AAA37673. (PubMed:2158861)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58288 mRNA. Translation: AAA37673.1.
    AL627101 Genomic DNA. Translation: CAM15033.1.
    CCDSiCCDS18640.1.
    PIRiA34898.
    RefSeqiNP_031808.2. NM_007782.3.
    XP_006502771.1. XM_006502708.1.
    XP_006502772.1. XM_006502709.1.
    XP_006502773.1. XM_006502710.1.
    XP_006502774.1. XM_006502711.1.
    XP_006502775.1. XM_006502712.1.
    XP_006502776.1. XM_006502713.1.
    XP_006502777.1. XM_006502714.1.
    UniGeneiMm.271701.

    Genome annotation databases

    EnsembliENSMUST00000030673; ENSMUSP00000030673; ENSMUSG00000028859.
    ENSMUST00000106162; ENSMUSP00000101768; ENSMUSG00000028859.
    GeneIDi12986.
    KEGGimmu:12986.
    UCSCiuc008usd.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58288 mRNA. Translation: AAA37673.1 .
    AL627101 Genomic DNA. Translation: CAM15033.1 .
    CCDSi CCDS18640.1.
    PIRi A34898.
    RefSeqi NP_031808.2. NM_007782.3.
    XP_006502771.1. XM_006502708.1.
    XP_006502772.1. XM_006502709.1.
    XP_006502773.1. XM_006502710.1.
    XP_006502774.1. XM_006502711.1.
    XP_006502775.1. XM_006502712.1.
    XP_006502776.1. XM_006502713.1.
    XP_006502777.1. XM_006502714.1.
    UniGenei Mm.271701.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CD9 X-ray 2.80 B/D 120-334 [» ]
    1CTO NMR - A 228-333 [» ]
    1GCF NMR - A 228-333 [» ]
    1PGR X-ray 3.50 B/D/F/H 120-334 [» ]
    ProteinModelPortali P40223.
    SMRi P40223. Positions 26-621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198935. 3 interactions.
    STRINGi 10090.ENSMUSP00000101768.

    PTM databases

    PhosphoSitei P40223.

    Proteomic databases

    PRIDEi P40223.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030673 ; ENSMUSP00000030673 ; ENSMUSG00000028859 .
    ENSMUST00000106162 ; ENSMUSP00000101768 ; ENSMUSG00000028859 .
    GeneIDi 12986.
    KEGGi mmu:12986.
    UCSCi uc008usd.3. mouse.

    Organism-specific databases

    CTDi 1441.
    MGIi MGI:1339755. Csf3r.

    Phylogenomic databases

    eggNOGi NOG29566.
    GeneTreei ENSGT00550000074436.
    HOGENOMi HOG000231142.
    HOVERGENi HBG051130.
    InParanoidi A2A8Y3.
    KOi K05061.
    OMAi YLRCDST.
    OrthoDBi EOG7FXZXN.
    TreeFami TF338122.

    Miscellaneous databases

    EvolutionaryTracei P40223.
    NextBioi 282776.
    PROi P40223.
    SOURCEi Search...

    Gene expression databases

    Bgeei P40223.
    Genevestigatori P40223.

    Family and domain databases

    Gene3Di 2.60.40.10. 5 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR003529. Hematopoietin_rcpt_Gp130_CS.
    IPR013783. Ig-like_fold.
    IPR010457. IgC2-like_lig-bd.
    [Graphical view ]
    Pfami PF00041. fn3. 1 hit.
    PF06328. Lep_receptor_Ig. 1 hit.
    [Graphical view ]
    SMARTi SM00060. FN3. 4 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 4 hits.
    PROSITEi PS50853. FN3. 5 hits.
    PS01353. HEMATOPO_REC_L_F2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning of a receptor for murine granulocyte colony-stimulating factor."
      Fukunaga R., Ishizaka-Ikeda E., Seto Y., Nagata S.
      Cell 61:341-350(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand."
      Yamasaki K., Naito S., Anaguchi H., Ohkubo T., Ota Y.
      Nat. Struct. Biol. 4:498-504(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 225-333.
    4. "Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme."
      Aritomi M., Kunishima N., Okamoto T., Kuroki R., Ota Y., Morikawa K.
      Nature 401:713-717(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 120-334 IN COMPLEX WITH CSF3, SUBUNIT, GLYCOSYLATION AT ASN-129, DISULFIDE BONDS.

    Entry informationi

    Entry nameiCSF3R_MOUSE
    AccessioniPrimary (citable) accession number: P40223
    Secondary accession number(s): A2A8Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3