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P40223 (CSF3R_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granulocyte colony-stimulating factor receptor

Short name=G-CSF receptor
Short name=G-CSF-R
Alternative name(s):
CD_antigen=CD114
Gene names
Name:Csf3r
Synonyms:Csfgr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for granulocyte colony-stimulating factor (CSF3). In addition it may function in some adhesion or recognition events at the cell surface.

Subunit structure

Homodimer. The dimeric receptor binds two CSF3 molecules. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Found in bone marrow.

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Contains 5 fibronectin type-III domains.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 837812Granulocyte colony-stimulating factor receptor
PRO_0000010875

Regions

Topological domain26 – 626601Extracellular Potential
Transmembrane627 – 65024Helical; Potential
Topological domain651 – 837187Cytoplasmic Potential
Domain26 – 11893Ig-like C2-type
Domain126 – 231106Fibronectin type-III 1
Domain236 – 33196Fibronectin type-III 2
Domain334 – 433100Fibronectin type-III 3
Domain434 – 52996Fibronectin type-III 4
Domain530 – 62495Fibronectin type-III 5
Motif319 – 3235WSXWS motif
Motif658 – 6669Box 1 motif

Amino acid modifications

Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation1291N-linked (GlcNAc...) Ref.4
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation2791N-linked (GlcNAc...) Potential
Glycosylation3921N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Glycosylation4741N-linked (GlcNAc...) Potential
Glycosylation4871N-linked (GlcNAc...) Potential
Glycosylation5821N-linked (GlcNAc...) Potential
Glycosylation6131N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 52 By similarity
Disulfide bond46 ↔ 102 By similarity
Disulfide bond132 ↔ 143 Ref.4
Disulfide bond168 ↔ 219 Ref.4
Disulfide bond178 ↔ 187 Ref.4
Disulfide bond249 ↔ 296 Ref.4
Disulfide bond267 ↔ 310 Ref.4

Experimental info

Sequence conflict3791S → N in AAA37673. Ref.1

Secondary structure

........................................ 837
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40223 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: F46E9D62A3DC4C3F

FASTA83793,379
        10         20         30         40         50         60 
MVGLGACTLT GVTLIFLLLP RSLESCGHIE ISPPVVRLGD PVLASCTISP NCSKLDQQAK 

        70         80         90        100        110        120 
ILWRLQDEPI QPGDRQHHLP DGTQESLITL PHLNYTQAFL FCLVPWEDSV QLLDQAELHA 

       130        140        150        160        170        180 
GYPPASPSNL SCLMHLTTNS LVCQWEPGPE THLPTSFILK SFRSRADCQY QGDTIPDCVA 

       190        200        210        220        230        240 
KKRQNNCSIP RKNLLLYQYM AIWVQAENML GSSESPKLCL DPMDVVKLEP PMLQALDIGP 

       250        260        270        280        290        300 
DVVSHQPGCL WLSWKPWKPS EYMEQECELR YQPQLKGANW TLVFHLPSSK DQFELCGLHQ 

       310        320        330        340        350        360 
APVYTLQMRC IRSSLPGFWS PWSPGLQLRP TMKAPTIRLD TWCQKKQLDP GTVSVQLFWK 

       370        380        390        400        410        420 
PTPLQEDSGQ IQGYLLSWSS PDHQGQDIHL CNTTQLSCIF LLPSEAQNVT LVAYNKAGTS 

       430        440        450        460        470        480 
SPTTVVFLEN EGPAVTGLHA MAQDLNTIWV DWEAPSLLPQ GYLIEWEMSS PSYNNSYKSW 

       490        500        510        520        530        540 
MIEPNGNITG ILLKDNINPF QLYRITVAPL YPGIVGPPVN VYTFAGERAP PHAPALHLKH 

       550        560        570        580        590        600 
VGTTWAQLEW VPEAPRLGMI PLTHYTIFWA DAGDHSFSVT LNISLHDFVL KHLEPASLYH 

       610        620        630        640        650        660 
VYLMATSRAG STNSTGLTLR TLDPSDLNIF LGILCLVLLS TTCVVTWLCC KRRGKTSFWS 

       670        680        690        700        710        720 
DVPDPAHSSL SSWLPTIMTE ETFQLPSFWD SSVPSITKIT ELEEDKKPTH WDSESSGNGS 

       730        740        750        760        770        780 
LPALVQAYVL QGDPREISNQ SQPPSRTGDQ VLYGQVLESP TSPGVMQYIR SDSTQPLLGG 

       790        800        810        820        830 
PTPSPKSYEN IWFHSRPQET FVPQPPNQED DCVFGPPFDF PLFQGLQVHG VEEQGGF 

« Hide

References

« Hide 'large scale' references
[1]"Expression cloning of a receptor for murine granulocyte colony-stimulating factor."
Fukunaga R., Ishizaka-Ikeda E., Seto Y., Nagata S.
Cell 61:341-350(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand."
Yamasaki K., Naito S., Anaguchi H., Ohkubo T., Ota Y.
Nat. Struct. Biol. 4:498-504(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 225-333.
[4]"Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme."
Aritomi M., Kunishima N., Okamoto T., Kuroki R., Ota Y., Morikawa K.
Nature 401:713-717(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 120-334 IN COMPLEX WITH CSF3, SUBUNIT, GLYCOSYLATION AT ASN-129, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58288 mRNA. Translation: AAA37673.1.
AL627101 Genomic DNA. Translation: CAM15033.1.
PIRA34898.
RefSeqNP_031808.2. NM_007782.3.
XP_006502771.1. XM_006502708.1.
XP_006502772.1. XM_006502709.1.
XP_006502773.1. XM_006502710.1.
XP_006502774.1. XM_006502711.1.
XP_006502775.1. XM_006502712.1.
XP_006502776.1. XM_006502713.1.
XP_006502777.1. XM_006502714.1.
UniGeneMm.271701.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CD9X-ray2.80B/D120-334[»]
1CTONMR-A228-333[»]
1GCFNMR-A228-333[»]
1PGRX-ray3.50B/D/F/H120-334[»]
ProteinModelPortalP40223.
SMRP40223. Positions 26-621.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198935. 3 interactions.
STRING10090.ENSMUSP00000101768.

PTM databases

PhosphoSiteP40223.

Proteomic databases

PRIDEP40223.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030673; ENSMUSP00000030673; ENSMUSG00000028859.
ENSMUST00000106162; ENSMUSP00000101768; ENSMUSG00000028859.
GeneID12986.
KEGGmmu:12986.
UCSCuc008usd.3. mouse.

Organism-specific databases

CTD1441.
MGIMGI:1339755. Csf3r.

Phylogenomic databases

eggNOGNOG29566.
GeneTreeENSGT00550000074436.
HOGENOMHOG000231142.
HOVERGENHBG051130.
InParanoidA2A8Y3.
KOK05061.
OMAYLRCDST.
OrthoDBEOG7FXZXN.
TreeFamTF338122.

Gene expression databases

BgeeP40223.
GenevestigatorP40223.

Family and domain databases

Gene3D2.60.40.10. 5 hits.
InterProIPR003961. Fibronectin_type3.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
[Graphical view]
PfamPF00041. fn3. 1 hit.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 4 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 4 hits.
PROSITEPS50853. FN3. 5 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP40223.
NextBio282776.
PROP40223.
SOURCESearch...

Entry information

Entry nameCSF3R_MOUSE
AccessionPrimary (citable) accession number: P40223
Secondary accession number(s): A2A8Y3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot