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Protein

Eukaryotic translation initiation factor 3 subunit I

Gene

TIF34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

GO - Molecular functioni

  • translation initiation factor activity Source: SGD

GO - Biological processi

  • cytoplasmic translational initiation Source: SGD
  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • regulation of translational initiation Source: UniProtKB-HAMAP
  • translational initiation Source: SGD
  • translation reinitiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-32838-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit IUniRule annotation
Short name:
eIF3iUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 39 kDa subunit
Short name:
eIF-3 39 kDa subunit
Short name:
eIF3 p39
Gene namesi
Name:TIF34UniRule annotation
Ordered Locus Names:YMR146C
ORF Names:YM9375.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR146C.
SGDiS000004754. TIF34.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Eukaryotic translation initiation factor 3 subunit IPRO_0000051041Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei302 – 3021PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40217.
PeptideAtlasiP40217.

PTM databases

iPTMnetiP40217.

Interactioni

Subunit structurei

The eukaryotic translation initiation factor 3 (eIF-3) core complex is composed of TIF32, PRT1, NIP1, TIF34 and TIF35. The factors eIF-1, eIF-2, eIF-3, TIF5/eIF-5 and methionyl-tRNAi form a multifactor complex (MFC) that may bind to the 40S ribosome.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRT1P0610314EBI-8951,EBI-8973
TIF35Q040676EBI-8951,EBI-8958

Protein-protein interaction databases

BioGridi35322. 59 interactions.
DIPiDIP-2520N.
IntActiP40217. 21 interactions.
MINTiMINT-489230.

Structurei

Secondary structure

347
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 77Combined sources
Beta strandi13 – 186Combined sources
Beta strandi24 – 329Combined sources
Beta strandi34 – 385Combined sources
Turni39 – 413Combined sources
Beta strandi44 – 485Combined sources
Beta strandi55 – 606Combined sources
Beta strandi64 – 718Combined sources
Beta strandi74 – 807Combined sources
Turni81 – 833Combined sources
Beta strandi86 – 916Combined sources
Beta strandi96 – 1016Combined sources
Beta strandi105 – 1128Combined sources
Beta strandi121 – 1299Combined sources
Turni131 – 1333Combined sources
Beta strandi136 – 1394Combined sources
Beta strandi144 – 1485Combined sources
Beta strandi156 – 1616Combined sources
Helixi163 – 1653Combined sources
Beta strandi167 – 1726Combined sources
Beta strandi175 – 1817Combined sources
Turni182 – 1865Combined sources
Beta strandi187 – 1937Combined sources
Beta strandi199 – 2046Combined sources
Beta strandi208 – 2158Combined sources
Beta strandi218 – 2247Combined sources
Turni225 – 2273Combined sources
Beta strandi230 – 2356Combined sources
Beta strandi240 – 2456Combined sources
Beta strandi247 – 25610Combined sources
Beta strandi267 – 2693Combined sources
Beta strandi274 – 2796Combined sources
Turni280 – 2823Combined sources
Beta strandi285 – 2906Combined sources
Beta strandi296 – 3016Combined sources
Beta strandi305 – 3128Combined sources
Beta strandi315 – 3228Combined sources
Helixi324 – 3274Combined sources
Helixi332 – 3398Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JAPelectron microscopy4.90q1-347[»]
3JAQelectron microscopy6.00q1-347[»]
3ZWLX-ray2.20B/D1-347[»]
5A5Uelectron microscopy9.00I1-347[»]
ProteinModelPortaliP40217.
SMRiP40217. Positions 1-342.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40217.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati8 – 4740WD 1Add
BLAST
Repeati50 – 8940WD 2Add
BLAST
Repeati149 – 19042WD 3Add
BLAST
Repeati194 – 23340WD 4Add
BLAST
Repeati291 – 33040WD 5Add
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit I family.UniRule annotation
Contains 5 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00630000089849.
HOGENOMiHOG000231322.
InParanoidiP40217.
KOiK03246.
OMAiHGIHNEE.
OrthoDBiEOG7Z95X9.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03008. eIF3i.
InterProiIPR027525. eIF3i.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG
60 70 80 90 100
HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVATW KSPVPVKRVE
110 120 130 140 150
FSPCGNYFLA ILDNVMKNPG SINIYEIERD SATHELTKVS EEPIHKIITH
160 170 180 190 200
EGLDAATVAG WSTKGKYIIA GHKDGKISKY DVSNNYEYVD SIDLHEKSIS
210 220 230 240 250
DMQFSPDLTY FITSSRDTNS FLVDVSTLQV LKKYETDCPL NTAVITPLKE
260 270 280 290 300
FIILGGGQEA KDVTTTSANE GKFEARFYHK IFEEEIGRVQ GHFGPLNTVA
310 320 330 340
ISPQGTSYAS GGEDGFIRLH HFEKSYFDFK YDVEKAAEAK EHMQEAN
Length:347
Mass (Da):38,755
Last modified:February 1, 1995 - v1
Checksum:iE6C0D0B275E87622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56937 Genomic DNA. Translation: AAC49616.1.
Z47071 Genomic DNA. Translation: CAA87361.1.
AY558416 Genomic DNA. Translation: AAS56742.1.
BK006946 Genomic DNA. Translation: DAA10042.1.
PIRiS50403.
RefSeqiNP_013866.1. NM_001182648.1.

Genome annotation databases

EnsemblFungiiYMR146C; YMR146C; YMR146C.
GeneIDi855177.
KEGGisce:YMR146C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56937 Genomic DNA. Translation: AAC49616.1.
Z47071 Genomic DNA. Translation: CAA87361.1.
AY558416 Genomic DNA. Translation: AAS56742.1.
BK006946 Genomic DNA. Translation: DAA10042.1.
PIRiS50403.
RefSeqiNP_013866.1. NM_001182648.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JAPelectron microscopy4.90q1-347[»]
3JAQelectron microscopy6.00q1-347[»]
3ZWLX-ray2.20B/D1-347[»]
5A5Uelectron microscopy9.00I1-347[»]
ProteinModelPortaliP40217.
SMRiP40217. Positions 1-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35322. 59 interactions.
DIPiDIP-2520N.
IntActiP40217. 21 interactions.
MINTiMINT-489230.

PTM databases

iPTMnetiP40217.

Proteomic databases

MaxQBiP40217.
PeptideAtlasiP40217.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR146C; YMR146C; YMR146C.
GeneIDi855177.
KEGGisce:YMR146C.

Organism-specific databases

EuPathDBiFungiDB:YMR146C.
SGDiS000004754. TIF34.

Phylogenomic databases

GeneTreeiENSGT00630000089849.
HOGENOMiHOG000231322.
InParanoidiP40217.
KOiK03246.
OMAiHGIHNEE.
OrthoDBiEOG7Z95X9.

Enzyme and pathway databases

BioCyciYEAST:G3O-32838-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

EvolutionaryTraceiP40217.
NextBioi978626.
PROiP40217.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03008. eIF3i.
InterProiIPR027525. eIF3i.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The 39-kilodalton subunit of eukaryotic translation initiation factor 3 is essential for the complex's integrity and for cell viability in Saccharomyces cerevisiae."
    Naranda T., Kainuma M., Macmillan S.E., Hershey J.W.B.
    Mol. Cell. Biol. 17:145-153(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W303D.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5."
    Phan L., Zhang X., Asano K., Anderson J., Vornlocher H.-P., Greenberg J.R., Qin J., Hinnebusch A.G.
    Mol. Cell. Biol. 18:4935-4946(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE EIF-3 CORE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast."
    Nielsen K.H., Valasek L., Sykes C., Jivotovskaya A., Hinnebusch A.G.
    Mol. Cell. Biol. 26:2984-2998(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRT1, ASSOCIATION WITH THE 40S RIBOSOME.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3."
    Zhou M., Sandercock A.M., Fraser C.S., Ridlova G., Stephens E., Schenauer M.R., Yokoi-Fong T., Barsky D., Leary J.A., Hershey J.W.B., Doudna J.A., Robinson C.V.
    Proc. Natl. Acad. Sci. U.S.A. 105:18139-18144(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE EIF-3 COMPLEX WITH NIP1; PRT1; TIF32 AND TIF35.

Entry informationi

Entry nameiEIF3I_YEAST
AccessioniPrimary (citable) accession number: P40217
Secondary accession number(s): D6VZW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.