Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic translation initiation factor 3 subunit I

Gene

TIF34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.UniRule annotation

Miscellaneous

Present with 2400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • translation initiation factor activity Source: SGD

GO - Biological processi

  • cytoplasmic translational initiation Source: SGD
  • translational initiation Source: SGD
  • translation reinitiation Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-32838-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit IUniRule annotation
Short name:
eIF3iUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 39 kDa subunit
Short name:
eIF-3 39 kDa subunit
Short name:
eIF3 p39
Gene namesi
Name:TIF34UniRule annotation
Ordered Locus Names:YMR146C
ORF Names:YM9375.16C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR146C
SGDiS000004754 TIF34

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510411 – 347Eukaryotic translation initiation factor 3 subunit IAdd BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40217
PaxDbiP40217
PRIDEiP40217

PTM databases

iPTMnetiP40217

Interactioni

Subunit structurei

The eukaryotic translation initiation factor 3 (eIF-3) core complex is composed of TIF32, PRT1, NIP1, TIF34 and TIF35. The factors eIF-1, eIF-2, eIF-3, TIF5/eIF-5 and methionyl-tRNAi form a multifactor complex (MFC) that may bind to the 40S ribosome.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35322, 357 interactors
DIPiDIP-2520N
IntActiP40217, 33 interactors
MINTiP40217
STRINGi4932.YMR146C

Structurei

Secondary structure

1347
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 7Combined sources7
Beta strandi13 – 18Combined sources6
Beta strandi24 – 32Combined sources9
Beta strandi34 – 38Combined sources5
Turni39 – 41Combined sources3
Beta strandi44 – 48Combined sources5
Beta strandi55 – 60Combined sources6
Beta strandi64 – 71Combined sources8
Beta strandi74 – 80Combined sources7
Turni81 – 83Combined sources3
Beta strandi86 – 91Combined sources6
Beta strandi96 – 101Combined sources6
Beta strandi105 – 112Combined sources8
Beta strandi121 – 129Combined sources9
Turni131 – 133Combined sources3
Beta strandi136 – 139Combined sources4
Beta strandi144 – 148Combined sources5
Beta strandi156 – 161Combined sources6
Helixi163 – 165Combined sources3
Beta strandi167 – 172Combined sources6
Beta strandi175 – 181Combined sources7
Turni182 – 186Combined sources5
Beta strandi187 – 193Combined sources7
Beta strandi199 – 204Combined sources6
Beta strandi208 – 215Combined sources8
Beta strandi218 – 224Combined sources7
Turni225 – 227Combined sources3
Beta strandi230 – 235Combined sources6
Beta strandi240 – 245Combined sources6
Beta strandi247 – 256Combined sources10
Beta strandi267 – 269Combined sources3
Beta strandi274 – 279Combined sources6
Turni280 – 282Combined sources3
Beta strandi285 – 290Combined sources6
Beta strandi296 – 301Combined sources6
Beta strandi305 – 312Combined sources8
Beta strandi315 – 322Combined sources8
Helixi324 – 327Combined sources4
Helixi332 – 339Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JAPelectron microscopy4.90q1-347[»]
3JAQelectron microscopy6.00q1-347[»]
3ZWLX-ray2.20B/D1-347[»]
5A5Uelectron microscopy9.00I1-347[»]
ProteinModelPortaliP40217
SMRiP40217
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40217

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati8 – 47WD 1Add BLAST40
Repeati50 – 89WD 2Add BLAST40
Repeati149 – 190WD 3Add BLAST42
Repeati194 – 233WD 4Add BLAST40
Repeati291 – 330WD 5Add BLAST40

Sequence similaritiesi

Belongs to the eIF-3 subunit I family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00630000089849
HOGENOMiHOG000231322
InParanoidiP40217
KOiK03246
OMAiFETRFWH
OrthoDBiEOG092C33OR

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03008 eIF3i, 1 hit
InterProiView protein in InterPro
IPR027525 eIF3i
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19877 PTHR19877, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

P40217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG
60 70 80 90 100
HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVATW KSPVPVKRVE
110 120 130 140 150
FSPCGNYFLA ILDNVMKNPG SINIYEIERD SATHELTKVS EEPIHKIITH
160 170 180 190 200
EGLDAATVAG WSTKGKYIIA GHKDGKISKY DVSNNYEYVD SIDLHEKSIS
210 220 230 240 250
DMQFSPDLTY FITSSRDTNS FLVDVSTLQV LKKYETDCPL NTAVITPLKE
260 270 280 290 300
FIILGGGQEA KDVTTTSANE GKFEARFYHK IFEEEIGRVQ GHFGPLNTVA
310 320 330 340
ISPQGTSYAS GGEDGFIRLH HFEKSYFDFK YDVEKAAEAK EHMQEAN
Length:347
Mass (Da):38,755
Last modified:February 1, 1995 - v1
Checksum:iE6C0D0B275E87622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56937 Genomic DNA Translation: AAC49616.1
Z47071 Genomic DNA Translation: CAA87361.1
AY558416 Genomic DNA Translation: AAS56742.1
BK006946 Genomic DNA Translation: DAA10042.1
PIRiS50403
RefSeqiNP_013866.1, NM_001182648.1

Genome annotation databases

EnsemblFungiiYMR146C; YMR146C; YMR146C
GeneIDi855177
KEGGisce:YMR146C

Similar proteinsi

Entry informationi

Entry nameiEIF3I_YEAST
AccessioniPrimary (citable) accession number: P40217
Secondary accession number(s): D6VZW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health