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Protein

External NADH-ubiquinone oxidoreductase 1, mitochondrial

Gene

NDE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

External NADH dehydrogenase required for optimum cellular growth with a number of nonfermentable carbon sources, including ethanol. With NDE2, performes the mitochondrial oxidation of cytosolic NADH under these growth conditions. Regulates the mitochondrial glycerol-3-phosphate dehydrogenase, GUT2, also involved in cytosolic NADH oxidation.6 Publications

Catalytic activityi

NADH + ubiquinone = NAD+ + ubiquinol.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi114 – 144FADBy similarityAdd BLAST31
Nucleotide bindingi275 – 311NADBy similarityAdd BLAST37

GO - Molecular functioni

  • NADH dehydrogenase activity Source: SGD

GO - Biological processi

  • chronological cell aging Source: SGD
  • glycolytic fermentation to ethanol Source: SGD
  • NADH oxidation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32837-MONOMER.
YEAST:G3O-32837-MONOMER.
SABIO-RKP40215.

Names & Taxonomyi

Protein namesi
Recommended name:
External NADH-ubiquinone oxidoreductase 1, mitochondrial (EC:1.6.5.9)
Alternative name(s):
External NADH dehydrogenase 1
Gene namesi
Name:NDE1
Synonyms:NDH1
Ordered Locus Names:YMR145C
ORF Names:YM9375.14C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR145C.
SGDiS000004753. NDE1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41MitochondrionSequence analysisAdd BLAST41
ChainiPRO_000020330642 – 560External NADH-ubiquinone oxidoreductase 1, mitochondrialAdd BLAST519

Proteomic databases

MaxQBiP40215.
PRIDEiP40215.

Interactioni

Protein-protein interaction databases

BioGridi35321. 53 interactors.
DIPiDIP-6528N.
IntActiP40215. 3 interactors.
MINTiMINT-695777.

Structurei

3D structure databases

ProteinModelPortaliP40215.
SMRiP40215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NADH dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000065152.
HOGENOMiHOG000182501.
InParanoidiP40215.
KOiK17871.
OMAiLVNKHDY.
OrthoDBiEOG092C2KAE.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRQSLMKTV WANSSRFSLQ SKSGLVKYAK NRSFHAARNL LEDKKVILQK
60 70 80 90 100
VAPTTGVVAK QSFFKRTGKF TLKALLYSAL AGTAYVSYSL YREANPSTQV
110 120 130 140 150
PQSDTFPNGS KRKTLVILGS GWGSVSLLKN LDTTLYNVVV VSPRNYFLFT
160 170 180 190 200
PLLPSTPVGT IELKSIVEPV RTIARRSHGE VHYYEAEAYD VDPENKTIKV
210 220 230 240 250
KSSAKNNDYD LDLKYDYLVV GVGAQPNTFG TPGVYEYSSF LKEISDAQEI
260 270 280 290 300
RLKIMSSIEK AASLSPKDPE RARLLSFVVV GGGPTGVEFA AELRDYVDQD
310 320 330 340 350
LRKWMPELSK EIKVTLVEAL PNILNMFDKY LVDYAQDLFK EEKIDLRLKT
360 370 380 390 400
MVKKVDATTI TAKTGDGDIE NIPYGVLVWA TGNAPREVSK NLMTKLEEQD
410 420 430 440 450
SRRGLLIDNK LQLLGAKGSI FAIGDCTFHP GLFPTAQVAH QEGEYLAQYF
460 470 480 490 500
KKAYKIDQLN WKMTHAKDDS EVARLKNQIV KTQSQIEDFK YNHKGALAYI
510 520 530 540 550
GSDKAIADLA VGEAKYRLAG SFTFLFWKSA YLAMCLSFRN RVLVAMDWAK
560
VYFLGRDSSI
Length:560
Mass (Da):62,774
Last modified:February 1, 1995 - v1
Checksum:i10B1795E12E29C34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47071 Genomic DNA. Translation: CAA87359.1.
AY692785 Genomic DNA. Translation: AAT92804.1.
BK006946 Genomic DNA. Translation: DAA10041.1.
PIRiS50401.
RefSeqiNP_013865.1. NM_001182647.1.

Genome annotation databases

EnsemblFungiiYMR145C; YMR145C; YMR145C.
GeneIDi855176.
KEGGisce:YMR145C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47071 Genomic DNA. Translation: CAA87359.1.
AY692785 Genomic DNA. Translation: AAT92804.1.
BK006946 Genomic DNA. Translation: DAA10041.1.
PIRiS50401.
RefSeqiNP_013865.1. NM_001182647.1.

3D structure databases

ProteinModelPortaliP40215.
SMRiP40215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35321. 53 interactors.
DIPiDIP-6528N.
IntActiP40215. 3 interactors.
MINTiMINT-695777.

Proteomic databases

MaxQBiP40215.
PRIDEiP40215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR145C; YMR145C; YMR145C.
GeneIDi855176.
KEGGisce:YMR145C.

Organism-specific databases

EuPathDBiFungiDB:YMR145C.
SGDiS000004753. NDE1.

Phylogenomic databases

GeneTreeiENSGT00530000065152.
HOGENOMiHOG000182501.
InParanoidiP40215.
KOiK17871.
OMAiLVNKHDY.
OrthoDBiEOG092C2KAE.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32837-MONOMER.
YEAST:G3O-32837-MONOMER.
SABIO-RKP40215.

Miscellaneous databases

PROiP40215.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNDH1_YEAST
AccessioniPrimary (citable) accession number: P40215
Secondary accession number(s): D6VZW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4930 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.