P40215 (NDH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
External NADH-ubiquinone oxidoreductase 1, mitochondrial
EC=1.6.5.3

Alternative name(s):
External NADH dehydrogenase 1

Gene names

Name:	NDE1
Synonyms:	NDH1
Ordered Locus Names:	YMR145C
ORF Names:	YM9375.14C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	External NADH dehydrogenase required for optimum cellular growth with a number of nonfermentable carbon sources, including ethanol. With NDE2, performes the mitochondrial oxidation of cytosolic NADH under these growth conditions. Regulates the mitochondrial glycerol-3-phosphate dehydrogenase, GUT2, also involved in cytosolic NADH oxydation. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol.
Subcellular location	Mitochondrion intermembrane space Ref.11.
Miscellaneous	Present with 4930 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the NADH dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from direct assay Ref.9. Source: SGD chronological cell aging Inferred from mutant phenotype. Source: SGD glucose catabolic process to ethanol Inferred from mutant phenotype Ref.5Ref.6. Source: SGD
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSA2	P10592	1	EBI-27294,EBI-8603

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 41	41	Mitochondrion Potential
	Chain	42 – 560	519	External NADH-ubiquinone oxidoreductase 1, mitochondrial		PRO_0000203306
Regions
	Nucleotide binding	114 – 144	31	FAD By similarity
	Nucleotide binding	275 – 311	37	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40215 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 10B1795E12E29C34	FASTA	560	62,774
	10 20 30 40 50 60 MIRQSLMKTV WANSSRFSLQ SKSGLVKYAK NRSFHAARNL LEDKKVILQK VAPTTGVVAK 70 80 90 100 110 120 QSFFKRTGKF TLKALLYSAL AGTAYVSYSL YREANPSTQV PQSDTFPNGS KRKTLVILGS 130 140 150 160 170 180 GWGSVSLLKN LDTTLYNVVV VSPRNYFLFT PLLPSTPVGT IELKSIVEPV RTIARRSHGE 190 200 210 220 230 240 VHYYEAEAYD VDPENKTIKV KSSAKNNDYD LDLKYDYLVV GVGAQPNTFG TPGVYEYSSF 250 260 270 280 290 300 LKEISDAQEI RLKIMSSIEK AASLSPKDPE RARLLSFVVV GGGPTGVEFA AELRDYVDQD 310 320 330 340 350 360 LRKWMPELSK EIKVTLVEAL PNILNMFDKY LVDYAQDLFK EEKIDLRLKT MVKKVDATTI 370 380 390 400 410 420 TAKTGDGDIE NIPYGVLVWA TGNAPREVSK NLMTKLEEQD SRRGLLIDNK LQLLGAKGSI 430 440 450 460 470 480 FAIGDCTFHP GLFPTAQVAH QEGEYLAQYF KKAYKIDQLN WKMTHAKDDS EVARLKNQIV 490 500 510 520 530 540 KTQSQIEDFK YNHKGALAYI GSDKAIADLA VGEAKYRLAG SFTFLFWKSA YLAMCLSFRN 550 560 RVLVAMDWAK VYFLGRDSSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: PROTEIN SEQUENCE OF 476-480, FUNCTION.
[5]	"Identification of a cytosolically directed NADH dehydrogenase in mitochondria of Saccharomyces cerevisiae." Small W.C., McAlister-Henn L. J. Bacteriol. 180:4051-4055(1998) [PubMed: 9696750] [Abstract] Cited for: FUNCTION.
[6]	"The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH." Luttik M.A.H., Overkamp K.M., Koetter P., de Vries S., van Dijken J.P., Pronk J.T. J. Biol. Chem. 273:24529-24534(1998) [PubMed: 9733747] [Abstract] Cited for: FUNCTION.
[7]	"In vivo analysis of the mechanisms for oxidation of cytosolic NADH by Saccharomyces cerevisiae mitochondria." Overkamp K.M., Bakker B.M., Koetter P., van Tuijl A., de Vries S., van Dijken J.P., Pronk J.T. J. Bacteriol. 182:2823-2830(2000) [PubMed: 10781551] [Abstract] Cited for: FUNCTION.
[8]	"Mitochondrial respiratory electron carriers are involved in oxidative stress during heat stress in Saccharomyces cerevisiae." Davidson J.F., Schiestl R.H. Mol. Cell. Biol. 21:8483-8489(2001) [PubMed: 11713283] [Abstract] Cited for: FUNCTION.
[9]	"Kinetic regulation of the mitochondrial glycerol-3-phosphate dehydrogenase by the external NADH dehydrogenase in Saccharomyces cerevisiae." Paahlman I.-L., Larsson C., Averet N., Bunoust O., Boubekeur S., Gustafsson L., Rigoulet M. J. Biol. Chem. 277:27991-27995(2002) [PubMed: 12032156] [Abstract] Cited for: FUNCTION.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47071 Genomic DNA. Translation: CAA87359.1. AY692785 Genomic DNA. Translation: AAT92804.1. BK006946 Genomic DNA. Translation: DAA10041.1.
PIR	S50401.
RefSeq	NP_013865.1. NM_001182647.1.
3D structure databases
ProteinModelPortal	P40215.
SMR	P40215. Positions 111-451.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6528N.
IntAct	P40215. 6 interactions.
MINT	MINT-695777.
STRING	P40215.
Proteomic databases
PeptideAtlas	P40215.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR145C; YMR145C; YMR145C.
GeneID	855176.
KEGG	sce:YMR145C.
NMPDR	fig|4932.3.peg.4915.
Organism-specific databases
CYGD	YMR145c.
SGD	S000004753. NDE1.
Phylogenomic databases
eggNOG	fuNOG04442.
GeneTree	EFGT00050000003551.
HOGENOM	HBG319217.
OMA	FTFLFWK.
OrthoDB	EOG4VT95D.
Gene expression databases
ArrayExpress	P40215.
Genevestigator	P40215.
GermOnline	YMR145C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
KO	K03885.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 2 hits. [Graphical view]
PRINTS	PR00368. FADPNR.
ProtoNet	Search...
Other
NextBio	978623.

Entry information

Entry name

NDH1_YEAST

Accession

Primary (citable) accession number: P40215
Secondary accession number(s): D6VZW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program