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Protein

Small nuclear ribonucleoprotein G

Gene

SMX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site.

Miscellaneous

Present with 1400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
  • spliceosomal snRNP assembly Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30503-MONOMER.
ReactomeiR-SCE-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Small nuclear ribonucleoprotein G
Short name:
snRNP-G
Alternative name(s):
Sm protein G
Short name:
Sm-G
Short name:
SmG
Gene namesi
Name:SMX2
Synonyms:SNP2
Ordered Locus Names:YFL017W-A
ORF Names:YFL017Bw
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL017W-A.
SGDiS000002965. SMX2.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • P granule Source: GO_Central
  • precatalytic spliceosome Source: GO_Central
  • SMN-Sm protein complex Source: GO_Central
  • U12-type spliceosomal complex Source: GO_Central
  • U1 snRNP Source: SGD
  • U2 snRNP Source: GO_Central
  • U2-type prespliceosome Source: SGD
  • U4/U6 x U5 tri-snRNP complex Source: SGD
  • U4 snRNP Source: GO_Central
  • U5 snRNP Source: SGD

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001255521 – 77Small nuclear ribonucleoprotein GAdd BLAST77

Proteomic databases

MaxQBiP40204.
PRIDEiP40204.
TopDownProteomicsiP40204.

Interactioni

Subunit structurei

Component of the Sm core complex, present in spliceosomal snRNP U1, U2, U4/U6 and U5. The core complex contains SMB1, SMD1, SMD2, SMD3, SME1, SMX3 and SMX2 (Sm proteins B, D1, D2, D3, E, F and G, respectively), and is probably a heptameric ring structure. SMX2 specifically interacts with SME1. Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SME1Q123305EBI-637,EBI-16359

Protein-protein interaction databases

BioGridi31129. 55 interactors.
DIPiDIP-6683N.
IntActiP40204. 57 interactors.
MINTiMINT-613464.
STRINGi4932.YFL017W-A.

Structurei

Secondary structure

177
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Beta strandi13 – 19Combined sources7
Turni20 – 22Combined sources3
Beta strandi23 – 25Combined sources3
Beta strandi28 – 32Combined sources5
Beta strandi38 – 41Combined sources4
Beta strandi59 – 63Combined sources5
Turni65 – 67Combined sources3
Beta strandi68 – 74Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80X/a1-77[»]
5GAMelectron microscopy3.70g1-77[»]
5GANelectron microscopy3.60g/r1-77[»]
5GAOelectron microscopy3.60r1-77[»]
5GM6electron microscopy3.50j1-77[»]
5GMKelectron microscopy3.40j/x1-77[»]
5LJ3electron microscopy3.80g/r1-77[»]
5LJ5electron microscopy3.80g/r1-77[»]
5LQWelectron microscopy5.80g1-77[»]
5MPSelectron microscopy3.85g1-77[»]
5MQ0electron microscopy4.17g/r1-77[»]
5NRLelectron microscopy7.20g/r/y1-77[»]
5WSGelectron microscopy4.00K/j1-77[»]
ProteinModelPortaliP40204.
SMRiP40204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the snRNP Sm proteins family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000046985.
HOGENOMiHOG000223546.
InParanoidiP40204.
KOiK11099.
OMAiGEKVPIG.
OrthoDBiEOG092C5VL3.

Family and domain databases

CDDicd01719. Sm_G. 1 hit.
InterProiView protein in InterPro
IPR010920. LSM_dom.
IPR001163. LSM_dom_euk/arc.
IPR034098. Sm_G.
PfamiView protein in Pfam
PF01423. LSM. 1 hit.
SMARTiView protein in SMART
SM00651. Sm. 1 hit.
SUPFAMiSSF50182. SSF50182. 1 hit.

Sequencei

Sequence statusi: Complete.

P40204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED
60 70
PANNHQLGLQ TVIRGNSIIS LEALDAI
Length:77
Mass (Da):8,482
Last modified:February 1, 1995 - v1
Checksum:i5C6208B8C5A9AB33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20880 Genomic DNA. No translation available.
L31794 Genomic DNA. Translation: AAB63977.1.
D50617 Genomic DNA. No translation available.
Z46255 Genomic DNA. Translation: CAA86353.1.
AY558463 Genomic DNA. Translation: AAS56789.1.
BK006940 Genomic DNA. Translation: DAA12422.1.
PIRiS48322.
RefSeqiNP_116636.1. NM_001180864.1.

Genome annotation databases

EnsemblFungiiYFL017W-A; YFL017W-A; YFL017W-A.
GeneIDi850528.
KEGGisce:YFL017W-A.

Similar proteinsi

Entry informationi

Entry nameiRUXG_YEAST
AccessioniPrimary (citable) accession number: P40204
Secondary accession number(s): D6VTL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: August 30, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names