P40204 (RUXG_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small nuclear ribonucleoprotein G
Short name=snRNP-G

Alternative name(s):
Sm protein G
Short name=Sm-G
Short name=SmG

Gene names

Name:	SMX2
Synonyms:	SNP2
Ordered Locus Names:	YFL017W-A
ORF Names:	YFL017Bw

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	77 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site.
Subunit structure	Component of the Sm core complex, present in spliceosomal snRNP U1, U2, U4/U6 and U5. The core complex contains SMB1, SMD1, SMD2, SMD3, SME1, SMX3 and SMX2 (Sm proteins B, D1, D2, D3, E, F and G, respectively), and is probably a heptameric ring structure. SMX2 specifically interacts with SME1. Ref.3 Ref.11
Subcellular location	Nucleus.
Miscellaneous	Present with 1400 molecules/cell in log phase SD medium. Ref.12
Sequence similarities	Belongs to the snRNP Sm proteins family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from physical interaction. Source: SGD
Cellular component	U1 snRNP Inferred from direct assay Ref.8. Source: SGD U2-type prespliceosome Inferred from direct assay. Source: SGD U4/U6 x U5 tri-snRNP complex Inferred from direct assay. Source: SGD U5 snRNP Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 77	77	Small nuclear ribonucleoprotein G		PRO_0000125552

Sequences

	Sequence		Length	Mass (Da)
	P40204 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 5C6208B8C5A9AB33	FASTA	77	8,482
	10 20 30 40 50 60 MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ 70 TVIRGNSIIS LEALDAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the LPD1 gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae: comparison between eukaryotic and prokaryotic sequences for related enzymes and identification of potential upstream control sites." Ross J., Reid G.A., Dawes I.W. J. Gen. Microbiol. 134:1131-1139(1988) [PubMed: 3058861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Kornfeld G.D. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Interactions within the yeast Sm core complex: from proteins to amino acids." Camasses A., Bragado-Nilsson E., Martin R., Seraphin B., Bordonne R. Mol. Cell. Biol. 18:1956-1966(1998) [PubMed: 9528767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH SME1.
[4]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	Barrell B.G., Churcher C., Rajandream M.A. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[7]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[8]	"Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry." Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M. Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed: 9012791] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[9]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract] Cited for: CHARACTERIZATION OF THE SPLICEOSOME.
[10]	"Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin." Salgado-Garrido J., Bragado-Nilsson E., Kandels-Lewis S., Seraphin B. EMBO J. 18:3451-3462(1999) [PubMed: 10369684] [Abstract] Cited for: RNA-BINDING.
[11]	"Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain." Walke S., Bragado-Nilsson E., Seraphin B., Nagai K. J. Mol. Biol. 308:49-58(2001) [PubMed: 11302706] [Abstract] Cited for: SUBUNIT.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20880 Genomic DNA. No translation available. L31794 Genomic DNA. Translation: AAB63977.1. D50617 Genomic DNA. No translation available. Z46255 Genomic DNA. Translation: CAA86353.1. AY558463 Genomic DNA. Translation: AAS56789.1. BK006940 Genomic DNA. Translation: DAA12422.1.
PIR	S48322.
RefSeq	NP_116636.1. NM_001180864.1.
3D structure databases
ProteinModelPortal	P40204.
SMR	P40204. Positions 3-77.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6683N.
IntAct	P40204. 57 interactions.
MINT	MINT-613464.
STRING	P40204.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL017W-A; YFL017W-A; YFL017W-A.
GeneID	850528.
KEGG	sce:YFL017W-A.
NMPDR	fig|4932.3.peg.2264.
Organism-specific databases
CYGD	YFL017w-a.
SGD	S000002965. SMX2.
Phylogenomic databases
eggNOG	fuNOG11063.
GeneTree	EFGT00050000000581.
HOGENOM	HBG749684.
OMA	VVIMEPK.
OrthoDB	EOG4XWK7N.
Gene expression databases
ArrayExpress	P40204.
Genevestigator	P40204.
GermOnline	YFL017W-A. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010920. LSM-related_domain. IPR001163. LSM_domain. IPR006649. LSM_domain_euk/arc. [Graphical view]
KO	K11099.
Pfam	PF01423. LSM. 1 hit. [Graphical view]
SMART	SM00651. Sm. 1 hit. [Graphical view]
SUPFAM	SSF50182. Sm_like_riboprot. 1 hit.
ProtoNet	Search...
Other
NextBio	966271.

Entry information

Entry name

RUXG_YEAST

Accession

Primary (citable) accession number: P40204
Secondary accession number(s): D6VTL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program