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P40201

- CHD1_MOUSE

UniProt

P40201 - CHD1_MOUSE

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Protein
Chromodomain-helicase-DNA-binding protein 1
Gene
Chd1, Chd-1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 By similarity. Required for maintaining open chromatin and pluripotency in embryonic stem cells. Is also associated with histone deacetylase (HDAC) activity.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi504 – 5118ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. chromatin binding Source: UniProtKB
  4. helicase activity Source: UniProtKB

GO - Biological processi

  1. chromatin assembly or disassembly Source: UniProtKB
  2. chromatin remodeling Source: MGI
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromodomain-helicase-DNA-binding protein 1 (EC:3.6.4.12)
Short name:
CHD-1
Alternative name(s):
ATP-dependent helicase CHD1
Gene namesi
Name:Chd1
Synonyms:Chd-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:88393. Chd1.

Subcellular locationi

Nucleus. Cytoplasm
Note: Is released into the cytoplasm when cells enter mitosis and is reincorporated into chromatin during telophase-cytokinesis.2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17111711Chromodomain-helicase-DNA-binding protein 1
PRO_0000080225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei214 – 2141Phosphoserine By similarity
Modified residuei215 – 2151Phosphoserine By similarity
Modified residuei235 – 2351Phosphothreonine By similarity
Modified residuei239 – 2391Phosphoserine By similarity
Modified residuei248 – 2481Phosphothreonine By similarity
Modified residuei250 – 2501Phosphoserine By similarity
Modified residuei1023 – 10231Phosphoserine By similarity
Modified residuei1038 – 10381Phosphoserine By similarity
Modified residuei1079 – 10791Phosphoserine By similarity
Modified residuei1094 – 10941Phosphoserine By similarity
Modified residuei1096 – 10961Phosphoserine By similarity
Modified residuei1098 – 10981Phosphoserine By similarity
Modified residuei1100 – 11001Phosphoserine By similarity
Modified residuei1353 – 13531Phosphoserine By similarity
Modified residuei1355 – 13551Phosphoserine By similarity
Modified residuei1356 – 13561Phosphoserine By similarity
Modified residuei1360 – 13601Phosphoserine By similarity
Modified residuei1363 – 13631Phosphoserine By similarity
Modified residuei1678 – 16781Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40201.
PaxDbiP40201.
PRIDEiP40201.

PTM databases

PhosphoSiteiP40201.

Expressioni

Tissue specificityi

Abundance is higher in cells representing early stages of the B-lymphoid lineage such as pre-B and B-cells, than in cells representing mature plasmacytes or other cell lineages such as fibroblasts.

Gene expression databases

ArrayExpressiP40201.
BgeeiP40201.
CleanExiMM_CHD1.
GenevestigatoriP40201.

Interactioni

Subunit structurei

Component of the SAGA complex By similarity. Specifically interacts with methylated H3K4me2 and H3K4me3. Interacts with the FACT complex, the PAF complex and the U2 snRNP. Interacts directly with PAF1, SFA3A1, SFA3A2, SFA3A3, SNF2 and SSRP1 By similarity. Interacts with BCLAF1, NCoR, SRP20 and SAFB.3 Publications

Protein-protein interaction databases

BioGridi198693. 2 interactions.
IntActiP40201. 2 interactions.
MINTiMINT-4090783.

Structurei

3D structure databases

ProteinModelPortaliP40201.
SMRiP40201. Positions 268-440, 1122-1327.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini270 – 36293Chromo 1
Add
BLAST
Domaini387 – 45064Chromo 2
Add
BLAST
Domaini491 – 661171Helicase ATP-binding
Add
BLAST
Domaini790 – 941152Helicase C-terminal
Add
BLAST
Repeati1629 – 163351
Repeati1635 – 163952
Repeati1641 – 164553

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1629 – 1645173 X 5 AA repeats of H-S-D-H-R
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi612 – 6154DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 7070Ser-rich
Add
BLAST
Compositional biasi116 – 13621Ser-rich
Add
BLAST

Domaini

The 2 chromodomains are involved in the binding to the histone H3 methyllysine at position 4 (H3K4me3) By similarity.1 Publication

Sequence similaritiesi

Contains 2 chromo domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00560000076896.
HOGENOMiHOG000207917.
HOVERGENiHBG005325.
InParanoidiQ14BJ0.
KOiK11367.
OMAiMDHRASS.
OrthoDBiEOG7NPFSB.
TreeFamiTF300674.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40201-1 [UniParc]FASTAAdd to Basket

« Hide

MNGHSDEESV RNGSGESSQS GDDCGSASGS GSGSSSGSSS DGSSSQSGSS     50
DSDSGSDSGS QSESESDTSR ENKVQAKPPK VDGAEFWKSS PSILAVQRSA 100
MLRKQPQQAQ QQRPASSNSG SEEDSSSSED SDDSSSGAKR KKHNDEDWQM 150
SGSGSPSQSG SDSESEEERD KSSCDGTESD YEPKNKVRSR KPQNRSKSKN 200
GKKILGQKKR QIDSSEDEDD EDYDNDKRSS RRQATVNVSY KEDEEMKTDS 250
DDLLEVCGED VPQPEDEEFE TIERVMDCRV GRKGATGATT TIYAVEADGD 300
PNAGFERNKE PGDIQYLIKW KGWSHIHNTW ETEETLKQQN VRGMKKLDNY 350
KKKDQETKRW LKNASPEDVE YYNCQQELTD DLHKQYQIVE RIIAHSNQKS 400
AAGLPDYYCK WQGLPYSECS WEDGALISKK FQTCIDEYFS RNQSKTTPFK 450
DCKVLKQRPR FVALKKQPSY IGGHEGLELR DYQLNGLNWL AHSWCKGNSC 500
ILADEMGLGK TIQTISFLNY LFHEHQLYGP FLLVVPLSTL TSWQREIQTW 550
ASQMNAVVYL GDINSRNMIR THEWMHPQTK RLKFNILLTT YEILLKDKAF 600
LGGLNWAFIG VDEAHRLKND DSLLYKTLID FKSNHRLLIT GTPLQNSLKE 650
LWSLLHFIMP EKFSSWEDFE EEHGKGREYG YASLHKELEP FLLRRVKKDV 700
EKSLPAKVEQ ILRMEMSALQ KQYYKWILTR NYKALSKGSK GSTSGFLNIM 750
MELKKCCNHC YLIKPPDNNE FYNKQEALQH LIRSSGKLIL LDKLLIRLRE 800
RGNRVLIFSQ MVRMLDILAE YLKYRQFPFQ RLDGSIKGEL RKQALDHFNA 850
EGSEDFCFLL STRAGGLGIN LASADTVVIF DSDWNPQNDL QAQARAHRIG 900
QKKQVNIYRL VTKGSVEEDI LERAKKKMVL DHLVIQRMDT TGKTVLHTGS 950
APSSSTPFNK EELSAILKFG AEELFKEPEG EEQEPQEMDI DEILKRAETH 1000
ENEPGPLSVG DELLSQFKVA NFSNMDEDDI ELEPERNSKN WEEIIPEEQR 1050
RRLEEEERQK ELEEIYMLPR MRNCAKQISF NGSEGRRSRS RRYSGSDSDS 1100
ISERKRPKKR GRPRTIPREN IKGFSDAEIR RFIKSYKKFG GPLERLDAIA 1150
RDAELVDKSE TDLRRLGELV HNGCVKALKD SSSGTERAGG RLGKVKGPTF 1200
RISGVQVNAK LVIAHEDELI PLHKSIPSDP EERKQYTIPC HTKAAHFDID 1250
WGKEDDSNLL IGIYEYGYGS WEMIKMDPDL SLTHKILPDD PDKKPQAKQL 1300
QTRADYLIKL LSRDLAKREA QRLCGAGGSK RRKTRAKKSK AMKSIKVKEE 1350
IKSDSSPLPS EKSDEDDDKL NDSKPESKDR SKKSVVSDAP VHITASGEPV 1400
PIAEESEELD QKTFSICKER MRPVKAALKQ LDRPEKGLSE REQLEHTRQC 1450
LIKIGDHITE CLKEYSNPEQ IKQWRKNLWI FVSKFTEFDA RKLHKLYKHA 1500
IKKRQESQQN SDQNSNVATT HVIRNPDMER LKENTNHDDS SRDSYSSDRH 1550
LSQYHDHHKD RHQGDSYKKS DSRKRPYSSF SNGKDHREWD HYRQDSRYYS 1600
DREKHRKLDD HRSREHRPSL EGGLKDRCHS DHRSHSDHRM HSDHRSSSEH 1650
THHKSSRDYR YLSDWQLDHR AASSGPRSPL DQRSPYGSRS PFEHSAEHRS 1700
TPEHTWSSRK T 1711
Length:1,711
Mass (Da):196,385
Last modified:July 27, 2011 - v3
Checksum:i51499636E943A856
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591S → L in AAB08486. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10410 mRNA. Translation: AAB08486.1.
CH466630 Genomic DNA. Translation: EDL20454.1.
BC115822 mRNA. Translation: AAI15823.1.
CCDSiCCDS28416.1.
PIRiA47392.
RefSeqiNP_031716.2. NM_007690.3.
UniGeneiMm.8137.

Genome annotation databases

EnsembliENSMUST00000024627; ENSMUSP00000024627; ENSMUSG00000023852.
GeneIDi12648.
KEGGimmu:12648.
UCSCiuc008aou.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10410 mRNA. Translation: AAB08486.1 .
CH466630 Genomic DNA. Translation: EDL20454.1 .
BC115822 mRNA. Translation: AAI15823.1 .
CCDSi CCDS28416.1.
PIRi A47392.
RefSeqi NP_031716.2. NM_007690.3.
UniGenei Mm.8137.

3D structure databases

ProteinModelPortali P40201.
SMRi P40201. Positions 268-440, 1122-1327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198693. 2 interactions.
IntActi P40201. 2 interactions.
MINTi MINT-4090783.

PTM databases

PhosphoSitei P40201.

Proteomic databases

MaxQBi P40201.
PaxDbi P40201.
PRIDEi P40201.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024627 ; ENSMUSP00000024627 ; ENSMUSG00000023852 .
GeneIDi 12648.
KEGGi mmu:12648.
UCSCi uc008aou.2. mouse.

Organism-specific databases

CTDi 1105.
MGIi MGI:88393. Chd1.

Phylogenomic databases

eggNOGi COG0553.
GeneTreei ENSGT00560000076896.
HOGENOMi HOG000207917.
HOVERGENi HBG005325.
InParanoidi Q14BJ0.
KOi K11367.
OMAi MDHRASS.
OrthoDBi EOG7NPFSB.
TreeFami TF300674.

Miscellaneous databases

NextBioi 281852.
PROi P40201.
SOURCEi Search...

Gene expression databases

ArrayExpressi P40201.
Bgeei P40201.
CleanExi MM_CHD1.
Genevestigatori P40201.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR023780. Chromo_domain.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
IPR025260. DUF4208.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view ]
Pfami PF00385. Chromo. 2 hits.
PF13907. DUF4208. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00298. CHROMO. 2 hits.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF54160. SSF54160. 2 hits.
PROSITEi PS00598. CHROMO_1. 2 hits.
PS50013. CHROMO_2. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A mammalian DNA-binding protein that contains a chromodomain and an SNF2/SWI2-like helicase domain."
    Delmas V., Stokes D.G., Perry R.P.
    Proc. Natl. Acad. Sci. U.S.A. 90:2414-2418(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "DNA-binding and chromatin localization properties of CHD1."
    Stokes D.G., Perry R.P.
    Mol. Cell. Biol. 15:2745-2753(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DNA-BINDING.
  5. "CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin."
    Kelley D.E., Stokes D.G., Perry R.P.
    Chromosoma 108:10-25(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH SSRP1.
  6. "CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins."
    Tai H.H., Geisterfer M., Bell J.C., Moniwa M., Davie J.R., Boucher L., McBurney M.W.
    Biochem. Biophys. Res. Commun. 308:170-176(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BCLAF1; NCOR; SRP20 AND SAF-B, FUNCTION.
  7. Cited for: FUNCTION, INTERACTION WITH CHROMATIN.

Entry informationi

Entry nameiCHD1_MOUSE
AccessioniPrimary (citable) accession number: P40201
Secondary accession number(s): Q14BJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi