P40200 (TACT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-cell surface protein tactile

Alternative name(s):
Cell surface antigen CD96
T cell-activated increased late expression protein
CD_antigen=CD96

Gene names

Name:

CD96

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
Subunit structure	Homodimer; disulfide-linked. Interacts with PVR. Ref.4
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
Developmental stage	Expressed at low levels on peripheral T-cells and is strongly up-regulated after activation, peaking 6 to 9 days after the activating stimulus.
Involvement in disease	C syndrome (CSYN) [MIM:211750]: A syndrome characterized by trigonocephaly, severe mental retardation, hypotonia, variable cardiac defects, redundant skin, and dysmorphic facial features, including upslanted palpebral fissures, epicanthal folds, depressed nasal bridge, and low-set, posteriorly rotated ears. Note: The disease is caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. Ref.6
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Chromosomal rearrangement Polymorphism
Disease	Craniosynostosis Disease mutation
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Traceable author statement Ref.1. Source: ProtInc immune response Traceable author statement Ref.1. Source: ProtInc regulation of immune response Traceable author statement. Source: Reactome
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P40200-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P40200-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-197: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 585	564	T-cell surface protein tactile		PRO_0000014970
Regions
	Topological domain	22 – 519	498	Extracellular Potential
	Transmembrane	520 – 540	21	Helical; Potential
	Topological domain	541 – 585	45	Cytoplasmic Potential
	Domain	38 – 125	88	Ig-like V-type 1
	Domain	156 – 238	83	Ig-like V-type 2
	Domain	269 – 375	107	Ig-like C2-type
	Compositional bias	370 – 502	133	Pro/Ser/Thr-rich
	Compositional bias	554 – 563	10	Pro-rich
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	156	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Glycosylation	277	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	278	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Glycosylation	368	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 118		Probable
	Disulfide bond	163 ↔ 247		Probable
	Disulfide bond	290 ↔ 355		Probable
Natural variations
	Alternative sequence	182 – 197	16	Missing in isoform 2.		VSP_029908
	Natural variant	142	1	A → P. Corresponds to variant rs2276872 [ dbSNP | Ensembl ].		VAR_021928
	Natural variant	280	1	T → M in CSYN. Ref.6		VAR_037578

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 2C00C2971CAB13EE	FASTA	585	65,634
	10 20 30 40 50 60 MEKKWKYCAV YYIIQIHFVK GVWEKTVNTE ENVYATLGSD VNLTCQTQTV GFFVQMQWSK 70 80 90 100 110 120 VTNKIDLIAV YHPQYGFYCA YGRPCESLVT FTETPENGSK WTLHLRNMSC SVSGRYECML 130 140 150 160 170 180 VLYPEGIQTK IYNLLIQTHV TADEWNSNHT IEIEINQTLE IPCFQNSSSK ISSEFTYAWS 190 200 210 220 230 240 VENSSTDSWV LLSKGIKEDN GTQETLISQN HLISNSTLLK DRVKLGTDYR LHLSPVQIFD 250 260 270 280 290 300 DGRKFSCHIR VGPNKILRSS TTVKVFAKPE IPVIVENNST DVLVERRFTC LLKNVFPKAN 310 320 330 340 350 360 ITWFIDGSFL HDEKEGIYIT NEERKGKDGF LELKSVLTRV HSNKPAQSDN LTIWCMALSP 370 380 390 400 410 420 VPGNKVWNIS SEKITFLLGS EISSTDPPLS VTESTLDTQP SPASSVSPAR YPATSSVTLV 430 440 450 460 470 480 DVSALRPNTT PQPSNSSMTT RGFNYPWTSS GTDTKKSVSR IPSETYSSSP SGAGSTLHDN 490 500 510 520 530 540 VFTSTARAFS EVPTTANGST KTNHVHITGI VVNKPKDGMS WPVIVAALLF CCMILFGLGV 550 560 570 580 RKWCQYQKEI MERPPPFKPP PPPIKYTCIQ EPNESDLPYH EMETL
	Isoform 2 [UniParc]. Checksum: DF2F3BEE356F3BF2	FASTA	569	63,888
	10 20 30 40 50 60 MEKKWKYCAV YYIIQIHFVK GVWEKTVNTE ENVYATLGSD VNLTCQTQTV GFFVQMQWSK 70 80 90 100 110 120 VTNKIDLIAV YHPQYGFYCA YGRPCESLVT FTETPENGSK WTLHLRNMSC SVSGRYECML 130 140 150 160 170 180 VLYPEGIQTK IYNLLIQTHV TADEWNSNHT IEIEINQTLE IPCFQNSSSK ISSEFTYAWS 190 200 210 220 230 240 VEDNGTQETL ISQNHLISNS TLLKDRVKLG TDYRLHLSPV QIFDDGRKFS CHIRVGPNKI 250 260 270 280 290 300 LRSSTTVKVF AKPEIPVIVE NNSTDVLVER RFTCLLKNVF PKANITWFID GSFLHDEKEG 310 320 330 340 350 360 IYITNEERKG KDGFLELKSV LTRVHSNKPA QSDNLTIWCM ALSPVPGNKV WNISSEKITF 370 380 390 400 410 420 LLGSEISSTD PPLSVTESTL DTQPSPASSV SPARYPATSS VTLVDVSALR PNTTPQPSNS 430 440 450 460 470 480 SMTTRGFNYP WTSSGTDTKK SVSRIPSETY SSSPSGAGST LHDNVFTSTA RAFSEVPTTA 490 500 510 520 530 540 NGSTKTNHVH ITGIVVNKPK DGMSWPVIVA ALLFCCMILF GLGVRKWCQY QKEIMERPPP 550 560 FKPPPPPIKY TCIQEPNESD LPYHEMETL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular cloning of tactile. A novel human T cell activation antigen that is a member of the Ig gene superfamily." Wang P.L., O'Farrell S., Clayberger C., Krensky A.M. J. Immunol. 148:2600-2608(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph node.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155)." Fuchs A., Cella M., Giurisato E., Shaw A.S., Colonna M. J. Immunol. 172:3994-3998(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PVR.
[5]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-277 AND ASN-278, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[6]	"Mutations in CD96, a member of the immunoglobulin superfamily, cause a form of the C (Opitz trigonocephaly) syndrome." Kaname T., Yanagi K., Chinen Y., Makita Y., Okamoto N., Maehara H., Owan I., Kanaya F., Kubota Y., Oike Y., Yamamoto T., Kurosawa K., Fukushima Y., Bohring A., Opitz J.M., Yoshiura K., Niikawa N., Naritomi K. Am. J. Hum. Genet. 81:835-841(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT CSYN MET-280.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88282 mRNA. Translation: AAA36662.1. AL833681 mRNA. Translation: CAI46155.1. CH471052 Genomic DNA. Translation: EAW79698.1.
IPI	IPI00027604. IPI00396403.
PIR	A46462.
RefSeq	NP_005807.1. NM_005816.4. NP_937839.1. NM_198196.2.
UniGene	Hs.142023.
3D structure databases
ProteinModelPortal	P40200.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40200. 1 interaction.
STRING	9606.ENSP00000283285.
PTM databases
PhosphoSite	P40200.
Polymorphism databases
DMDM	161784352.
Proteomic databases
PaxDb	P40200.
PRIDE	P40200.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283285; ENSP00000283285; ENSG00000153283. ENST00000352690; ENSP00000342040; ENSG00000153283.
GeneID	10225.
KEGG	hsa:10225.
UCSC	uc003dxw.3. human. uc003dxx.3. human.
Organism-specific databases
CTD	10225.
GeneCards	GC03P111260.
HGNC	HGNC:16892. CD96.
MIM	211750. phenotype. 606037. gene.
neXtProt	NX_P40200.
Orphanet	1308. C syndrome.
PharmGKB	PA437.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG29658.
HOGENOM	HOG000150673.
HOVERGEN	HBG004030.
InParanoid	P40200.
KO	K06517.
OMA	NNLTIWC.
PhylomeDB	P40200.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P40200.
Bgee	P40200.
CleanEx	HS_CD96.
Genevestigator	P40200.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. [Graphical view]
SMART	SM00409. IG. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10225.
NextBio	38716.
SOURCE	Search...

Entry information

Entry name

TACT_HUMAN

Accession

Primary (citable) accession number: P40200
Secondary accession number(s): Q5JPB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.