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P40200 (TACT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell surface protein tactile
Alternative name(s):
Cell surface antigen CD96
T cell-activated increased late expression protein
CD_antigen=CD96
Gene names
Name:CD96
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.

Subunit structure

Homodimer; disulfide-linked. Interacts with PVR. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.

Developmental stage

Expressed at low levels on peripheral T-cells and is strongly up-regulated after activation, peaking 6 to 9 days after the activating stimulus.

Involvement in disease

C syndrome (CSYN) [MIM:211750]: A syndrome characterized by trigonocephaly, severe mental retardation, hypotonia, variable cardiac defects, redundant skin, and dysmorphic facial features, including upslanted palpebral fissures, epicanthal folds, depressed nasal bridge, and low-set, posteriorly rotated ears.
Note: The disease is caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. Ref.6

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P40200-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P40200-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-197: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 585564T-cell surface protein tactile
PRO_0000014970

Regions

Topological domain22 – 519498Extracellular Potential
Transmembrane520 – 54021Helical; Potential
Topological domain541 – 58545Cytoplasmic Potential
Domain38 – 12588Ig-like V-type 1
Domain156 – 23883Ig-like V-type 2
Domain269 – 375107Ig-like C2-type
Compositional bias370 – 502133Pro/Ser/Thr-rich
Compositional bias554 – 56310Pro-rich

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1071N-linked (GlcNAc...) Potential
Glycosylation1481N-linked (GlcNAc...) Potential
Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Ref.5
Glycosylation2781N-linked (GlcNAc...) Ref.5
Glycosylation3001N-linked (GlcNAc...) Potential
Glycosylation3501N-linked (GlcNAc...) Potential
Glycosylation3681N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential
Glycosylation4971N-linked (GlcNAc...) Potential
Disulfide bond45 ↔ 118 Probable
Disulfide bond163 ↔ 247 Probable
Disulfide bond290 ↔ 355 Probable

Natural variations

Alternative sequence182 – 19716Missing in isoform 2.
VSP_029908
Natural variant1421A → P.
Corresponds to variant rs2276872 [ dbSNP | Ensembl ].
VAR_021928
Natural variant2801T → M in CSYN. Ref.6
VAR_037578

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 2C00C2971CAB13EE

FASTA58565,634
        10         20         30         40         50         60 
MEKKWKYCAV YYIIQIHFVK GVWEKTVNTE ENVYATLGSD VNLTCQTQTV GFFVQMQWSK 

        70         80         90        100        110        120 
VTNKIDLIAV YHPQYGFYCA YGRPCESLVT FTETPENGSK WTLHLRNMSC SVSGRYECML 

       130        140        150        160        170        180 
VLYPEGIQTK IYNLLIQTHV TADEWNSNHT IEIEINQTLE IPCFQNSSSK ISSEFTYAWS 

       190        200        210        220        230        240 
VENSSTDSWV LLSKGIKEDN GTQETLISQN HLISNSTLLK DRVKLGTDYR LHLSPVQIFD 

       250        260        270        280        290        300 
DGRKFSCHIR VGPNKILRSS TTVKVFAKPE IPVIVENNST DVLVERRFTC LLKNVFPKAN 

       310        320        330        340        350        360 
ITWFIDGSFL HDEKEGIYIT NEERKGKDGF LELKSVLTRV HSNKPAQSDN LTIWCMALSP 

       370        380        390        400        410        420 
VPGNKVWNIS SEKITFLLGS EISSTDPPLS VTESTLDTQP SPASSVSPAR YPATSSVTLV 

       430        440        450        460        470        480 
DVSALRPNTT PQPSNSSMTT RGFNYPWTSS GTDTKKSVSR IPSETYSSSP SGAGSTLHDN 

       490        500        510        520        530        540 
VFTSTARAFS EVPTTANGST KTNHVHITGI VVNKPKDGMS WPVIVAALLF CCMILFGLGV 

       550        560        570        580 
RKWCQYQKEI MERPPPFKPP PPPIKYTCIQ EPNESDLPYH EMETL 

« Hide

Isoform 2 [UniParc].

Checksum: DF2F3BEE356F3BF2
Show »

FASTA56963,888

References

« Hide 'large scale' references
[1]"Identification and molecular cloning of tactile. A novel human T cell activation antigen that is a member of the Ig gene superfamily."
Wang P.L., O'Farrell S., Clayberger C., Krensky A.M.
J. Immunol. 148:2600-2608(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph node.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155)."
Fuchs A., Cella M., Giurisato E., Shaw A.S., Colonna M.
J. Immunol. 172:3994-3998(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PVR.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-277 AND ASN-278.
Tissue: Leukemic T-cell.
[6]"Mutations in CD96, a member of the immunoglobulin superfamily, cause a form of the C (Opitz trigonocephaly) syndrome."
Kaname T., Yanagi K., Chinen Y., Makita Y., Okamoto N., Maehara H., Owan I., Kanaya F., Kubota Y., Oike Y., Yamamoto T., Kurosawa K., Fukushima Y., Bohring A., Opitz J.M., Yoshiura K., Niikawa N., Naritomi K.
Am. J. Hum. Genet. 81:835-841(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT CSYN MET-280.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M88282 mRNA. Translation: AAA36662.1.
AL833681 mRNA. Translation: CAI46155.1.
CH471052 Genomic DNA. Translation: EAW79698.1.
PIRA46462.
RefSeqNP_005807.1. NM_005816.4.
NP_937839.1. NM_198196.2.
UniGeneHs.142023.

3D structure databases

ProteinModelPortalP40200.
SMRP40200. Positions 30-126.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP40200. 1 interaction.
STRING9606.ENSP00000283285.

PTM databases

PhosphoSiteP40200.

Polymorphism databases

DMDM161784352.

Proteomic databases

PaxDbP40200.
PRIDEP40200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000283285; ENSP00000283285; ENSG00000153283. [P40200-1]
ENST00000352690; ENSP00000342040; ENSG00000153283. [P40200-2]
GeneID10225.
KEGGhsa:10225.
UCSCuc003dxw.3. human. [P40200-1]
uc003dxx.3. human. [P40200-2]

Organism-specific databases

CTD10225.
GeneCardsGC03P111012.
HGNCHGNC:16892. CD96.
MIM211750. phenotype.
606037. gene.
neXtProtNX_P40200.
Orphanet1308. C syndrome.
PharmGKBPA437.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG29658.
HOGENOMHOG000150673.
HOVERGENHBG004030.
InParanoidP40200.
KOK06517.
OMAVQMQWSK.
OrthoDBEOG74R1QD.
PhylomeDBP40200.
TreeFamTF336934.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP40200.
BgeeP40200.
CleanExHS_CD96.
GenevestigatorP40200.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTSM00409. IG. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi10225.
NextBio38716.
PROP40200.
SOURCESearch...

Entry information

Entry nameTACT_HUMAN
AccessionPrimary (citable) accession number: P40200
Secondary accession number(s): Q5JPB3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries