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P40200

- TACT_HUMAN

UniProt

P40200 - TACT_HUMAN

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Protein

T-cell surface protein tactile

Gene

CD96

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.

GO - Biological processi

  1. cell adhesion Source: ProtInc
  2. immune response Source: ProtInc
  3. regulation of immune response Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface protein tactile
Alternative name(s):
Cell surface antigen CD96
T cell-activated increased late expression protein
CD_antigen: CD96
Gene namesi
Name:CD96
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:16892. CD96.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 519498ExtracellularSequence AnalysisAdd
BLAST
Transmembranei520 – 54021HelicalSequence AnalysisAdd
BLAST
Topological domaini541 – 58545CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

C syndrome (CSYN) [MIM:211750]: A syndrome characterized by trigonocephaly, severe mental retardation, hypotonia, variable cardiac defects, redundant skin, and dysmorphic facial features, including upslanted palpebral fissures, epicanthal folds, depressed nasal bridge, and low-set, posteriorly rotated ears.1 Publication
Note: The disease is caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint.
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti280 – 2801T → M in CSYN. 1 Publication
VAR_037578

Keywords - Diseasei

Craniosynostosis, Disease mutation

Organism-specific databases

MIMi211750. phenotype.
Orphaneti1308. C syndrome.
PharmGKBiPA437.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 585564T-cell surface protein tactilePRO_0000014970Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi45 ↔ 118Curated
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi148 – 1481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi163 ↔ 247Curated
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi277 – 2771N-linked (GlcNAc...)1 Publication
Glycosylationi278 – 2781N-linked (GlcNAc...)1 Publication
Disulfide bondi290 ↔ 355Curated
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP40200.
PRIDEiP40200.

PTM databases

PhosphoSiteiP40200.

Expressioni

Tissue specificityi

Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.

Developmental stagei

Expressed at low levels on peripheral T-cells and is strongly up-regulated after activation, peaking 6 to 9 days after the activating stimulus.

Gene expression databases

BgeeiP40200.
CleanExiHS_CD96.
ExpressionAtlasiP40200. baseline and differential.
GenevestigatoriP40200.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with PVR.1 Publication

Protein-protein interaction databases

BioGridi115519. 12 interactions.
IntActiP40200. 1 interaction.
STRINGi9606.ENSP00000283285.

Structurei

3D structure databases

ProteinModelPortaliP40200.
SMRiP40200. Positions 30-126, 217-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 12588Ig-like V-type 1Add
BLAST
Domaini156 – 23883Ig-like V-type 2Add
BLAST
Domaini269 – 375107Ig-like C2-typeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi370 – 502133Pro/Ser/Thr-richAdd
BLAST
Compositional biasi554 – 56310Pro-rich

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG29658.
GeneTreeiENSGT00390000003446.
HOGENOMiHOG000150673.
HOVERGENiHBG004030.
InParanoidiP40200.
KOiK06517.
OMAiVQMQWSK.
OrthoDBiEOG74R1QD.
PhylomeDBiP40200.
TreeFamiTF336934.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P40200-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKKWKYCAV YYIIQIHFVK GVWEKTVNTE ENVYATLGSD VNLTCQTQTV
60 70 80 90 100
GFFVQMQWSK VTNKIDLIAV YHPQYGFYCA YGRPCESLVT FTETPENGSK
110 120 130 140 150
WTLHLRNMSC SVSGRYECML VLYPEGIQTK IYNLLIQTHV TADEWNSNHT
160 170 180 190 200
IEIEINQTLE IPCFQNSSSK ISSEFTYAWS VENSSTDSWV LLSKGIKEDN
210 220 230 240 250
GTQETLISQN HLISNSTLLK DRVKLGTDYR LHLSPVQIFD DGRKFSCHIR
260 270 280 290 300
VGPNKILRSS TTVKVFAKPE IPVIVENNST DVLVERRFTC LLKNVFPKAN
310 320 330 340 350
ITWFIDGSFL HDEKEGIYIT NEERKGKDGF LELKSVLTRV HSNKPAQSDN
360 370 380 390 400
LTIWCMALSP VPGNKVWNIS SEKITFLLGS EISSTDPPLS VTESTLDTQP
410 420 430 440 450
SPASSVSPAR YPATSSVTLV DVSALRPNTT PQPSNSSMTT RGFNYPWTSS
460 470 480 490 500
GTDTKKSVSR IPSETYSSSP SGAGSTLHDN VFTSTARAFS EVPTTANGST
510 520 530 540 550
KTNHVHITGI VVNKPKDGMS WPVIVAALLF CCMILFGLGV RKWCQYQKEI
560 570 580
MERPPPFKPP PPPIKYTCIQ EPNESDLPYH EMETL
Length:585
Mass (Da):65,634
Last modified:December 4, 2007 - v2
Checksum:i2C00C2971CAB13EE
GO
Isoform 2 (identifier: P40200-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-197: Missing.

Show »
Length:569
Mass (Da):63,888
Checksum:iDF2F3BEE356F3BF2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti142 – 1421A → P.
Corresponds to variant rs2276872 [ dbSNP | Ensembl ].
VAR_021928
Natural varianti280 – 2801T → M in CSYN. 1 Publication
VAR_037578

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei182 – 19716Missing in isoform 2. 1 PublicationVSP_029908Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88282 mRNA. Translation: AAA36662.1.
AL833681 mRNA. Translation: CAI46155.1.
CH471052 Genomic DNA. Translation: EAW79698.1.
CCDSiCCDS2958.1. [P40200-2]
CCDS2959.1. [P40200-1]
PIRiA46462.
RefSeqiNP_005807.1. NM_005816.4. [P40200-2]
NP_937839.1. NM_198196.2. [P40200-1]
UniGeneiHs.142023.

Genome annotation databases

EnsembliENST00000283285; ENSP00000283285; ENSG00000153283. [P40200-1]
ENST00000352690; ENSP00000342040; ENSG00000153283. [P40200-2]
GeneIDi10225.
KEGGihsa:10225.
UCSCiuc003dxw.3. human. [P40200-1]
uc003dxx.3. human. [P40200-2]

Polymorphism databases

DMDMi161784352.

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88282 mRNA. Translation: AAA36662.1 .
AL833681 mRNA. Translation: CAI46155.1 .
CH471052 Genomic DNA. Translation: EAW79698.1 .
CCDSi CCDS2958.1. [P40200-2 ]
CCDS2959.1. [P40200-1 ]
PIRi A46462.
RefSeqi NP_005807.1. NM_005816.4. [P40200-2 ]
NP_937839.1. NM_198196.2. [P40200-1 ]
UniGenei Hs.142023.

3D structure databases

ProteinModelPortali P40200.
SMRi P40200. Positions 30-126, 217-361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115519. 12 interactions.
IntActi P40200. 1 interaction.
STRINGi 9606.ENSP00000283285.

PTM databases

PhosphoSitei P40200.

Polymorphism databases

DMDMi 161784352.

Proteomic databases

PaxDbi P40200.
PRIDEi P40200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000283285 ; ENSP00000283285 ; ENSG00000153283 . [P40200-1 ]
ENST00000352690 ; ENSP00000342040 ; ENSG00000153283 . [P40200-2 ]
GeneIDi 10225.
KEGGi hsa:10225.
UCSCi uc003dxw.3. human. [P40200-1 ]
uc003dxx.3. human. [P40200-2 ]

Organism-specific databases

CTDi 10225.
GeneCardsi GC03P111012.
HGNCi HGNC:16892. CD96.
MIMi 211750. phenotype.
606037. gene.
neXtProti NX_P40200.
Orphaneti 1308. C syndrome.
PharmGKBi PA437.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG29658.
GeneTreei ENSGT00390000003446.
HOGENOMi HOG000150673.
HOVERGENi HBG004030.
InParanoidi P40200.
KOi K06517.
OMAi VQMQWSK.
OrthoDBi EOG74R1QD.
PhylomeDBi P40200.
TreeFami TF336934.

Enzyme and pathway databases

Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

GenomeRNAii 10225.
NextBioi 38716.
PROi P40200.
SOURCEi Search...

Gene expression databases

Bgeei P40200.
CleanExi HS_CD96.
ExpressionAtlasi P40200. baseline and differential.
Genevestigatori P40200.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view ]
SMARTi SM00409. IG. 2 hits.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular cloning of tactile. A novel human T cell activation antigen that is a member of the Ig gene superfamily."
    Wang P.L., O'Farrell S., Clayberger C., Krensky A.M.
    J. Immunol. 148:2600-2608(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph node.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155)."
    Fuchs A., Cella M., Giurisato E., Shaw A.S., Colonna M.
    J. Immunol. 172:3994-3998(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PVR.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-277 AND ASN-278.
    Tissue: Leukemic T-cell.
  6. "Mutations in CD96, a member of the immunoglobulin superfamily, cause a form of the C (Opitz trigonocephaly) syndrome."
    Kaname T., Yanagi K., Chinen Y., Makita Y., Okamoto N., Maehara H., Owan I., Kanaya F., Kubota Y., Oike Y., Yamamoto T., Kurosawa K., Fukushima Y., Bohring A., Opitz J.M., Yoshiura K., Niikawa N., Naritomi K.
    Am. J. Hum. Genet. 81:835-841(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT CSYN MET-280.

Entry informationi

Entry nameiTACT_HUMAN
AccessioniPrimary (citable) accession number: P40200
Secondary accession number(s): Q5JPB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 4, 2007
Last modified: November 26, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3