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P40198 (CEAM3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carcinoembryonic antigen-related cell adhesion molecule 3
Alternative name(s):
Carcinoembryonic antigen CGM1
CD_antigen=CD66d
Gene names
Name:CEACAM3
Synonyms:CD66D, CGM1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis. Ref.8 Ref.9

Subunit structure

Interacts with S100A9/calprotectin. This interaction is calcium-dependent, but independent of CEACAM3 phosphorylation. Ref.7

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

CGM1a, the predominant CGM1 transcript, is granulocyte-specific. Not detected out of the granulocytic lineage, such as monocytes, lymphocytes, spleen, testis, colon, brain, liver, pancreas, thymus, ovary, placenta, skeletal muscle, prostate, small intestine, heart, lung and kidney. Ref.7

Domain

The cytosolic domain is involved in S100A9 interaction.

Post-translational modification

Tyrosine-phosphorylated in response to microbial binding. Tyr-230 and Tyr-241 are both required for phosphorylation to be detected. Ref.8

Sequence similarities

Belongs to the immunoglobulin superfamily. CEA family.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Caution

This is not the ortholog of rat CEACAM3.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P40198-1)

Also known as: CGM1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P40198-2)

Also known as: CGM1B;

The sequence of this isoform differs from the canonical sequence as follows:
     142-252: QENAPGLPVG...RMDHKAEVAS → PELPKPSISS...EPEIHSTTCL
Isoform 3 (identifier: P40198-3)

Also known as: CGM1C;

The sequence of this isoform differs from the canonical sequence as follows:
     182-252: TSIQRDLKEQ...RMDHKAEVAS → PWSLPQLCLLDVPSLHCLGPPTQPQDSSFHL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 By similarity
Chain35 – 252218Carcinoembryonic antigen-related cell adhesion molecule 3
PRO_0000014565

Regions

Topological domain35 – 155121Extracellular Potential
Transmembrane156 – 17621Helical; Potential
Topological domain177 – 25276Cytoplasmic Potential
Domain35 – 142108Ig-like V-type

Amino acid modifications

Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence142 – 252111QENAP…AEVAS → PELPKPSISSNNSNPKEDKD AVAFTCEPEIHSTTCL in isoform 2.
VSP_002486
Alternative sequence182 – 25271TSIQR…AEVAS → PWSLPQLCLLDVPSLHCLGP PTQPQDSSFHL in isoform 3.
VSP_002487
Natural variant71S → P. Ref.1
Corresponds to variant rs1041999 [ dbSNP | Ensembl ].
VAR_003905

Experimental info

Mutagenesis2301Y → F: Loss of phosphorylation and 30% reduction in bacterial uptake. More than 60% reduction in bacterial uptake and loss of RAC1 stimulation; when associated with F-241. Ref.8 Ref.9
Mutagenesis2411Y → F: Loss of phosphorylation and 30% reduction in bacterial uptake. More than 60% reduction in bacterial uptake and loss of RAC1 stimulation; when associated with F-230. Ref.8 Ref.9
Sequence conflict491K → N in BAA14321. Ref.1
Sequence conflict841I → T in BAA14322. Ref.1
Sequence conflict1371Q → H in BAA14322. Ref.1
Sequence conflict2151T → S in AAA51969. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CGM1A) [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 179F0C51C6A062A1

FASTA25227,091
        10         20         30         40         50         60 
MGPPSASPHR ECIPWQGLLL TASLLNFWNP PTTAKLTIES MPLSVAEGKE VLLLVHNLPQ 

        70         80         90        100        110        120 
HLFGYSWYKG ERVDGNSLIV GYVIGTQQAT PGAAYSGRET IYTNASLLIQ NVTQNDIGFY 

       130        140        150        160        170        180 
TLQVIKSDLV NEEATGQFHV YQENAPGLPV GAVAGIVTGV LVGVALVAAL VCFLLLAKTG 

       190        200        210        220        230        240 
RTSIQRDLKE QQPQALAPGR GPSHSSAFSM SPLSTAQAPL PNPRTAASIY EELLKHDTNI 

       250 
YCRMDHKAEV AS 

« Hide

Isoform 2 (CGM1B) [UniParc].

Checksum: C0BF243D56686F8C
Show »

FASTA17719,403
Isoform 3 (CGM1C) [UniParc].

Checksum: A146AF256C846762
Show »

FASTA21222,770

References

« Hide 'large scale' references
[1]"Molecular cloning of nonspecific cross-reacting antigens in human granulocytes."
Kuroki M., Arakawa F., Matsuo Y., Oikawa S., Misumi Y., Nakazato H., Matsuoka Y.
J. Biol. Chem. 266:11810-11817(1991) [PubMed: 2050678] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT PRO-7.
Tissue: Leukocyte.
[2]"Genomic organization, splice variants and expression of CGM1, a CD66-related member of the carcinoembryonic antigen gene family."
Nagel G., Grunert F., Kuijpers T.W., Watt S.M., Thompson J., Zimmermann W.A.
Eur. J. Biochem. 214:27-35(1993) [PubMed: 8508798] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Identification of three new genes and estimation of the size of the carcinoembryonic antigen family."
Khan W.N., Fraengsmyr L., Teglund S., Israelsson A., Bremer K., Hammarstroem S.
Genomics 14:384-390(1992) [PubMed: 1427854] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-141.
[7]"The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes."
Streichert T., Ebrahimnejad A., Ganzer S., Flayeh R., Wagener C., Bruemmer J.
Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed: 11708798] [Abstract]
Cited for: INTERACTION WITH S100A9, TISSUE SPECIFICITY.
[8]"Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor."
McCaw S.E., Schneider J., Liao E.H., Zimmermann W., Gray-Owen S.D.
Mol. Microbiol. 49:623-637(2003) [PubMed: 12864848] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF TYR-230 AND TYR-241.
[9]"Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens."
Schmitter T., Agerer F., Peterson L., Muenzner P., Hauck C.R.
J. Exp. Med. 199:35-46(2004) [PubMed: 14707113] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TYR-230 AND TYR-241.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90277 mRNA. Translation: BAA14321.1.
D90278 mRNA. Translation: BAA14322.1.
L00692 mRNA. Translation: AAA51969.1.
L00693 mRNA. Translation: AAA51970.1.
AC022516 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW57063.1.
BC106728 mRNA. Translation: AAI06729.1.
IPIIPI00027411.
IPI00289794.
IPI00411778.
PIRC40428.
S33323.
S33324.
RefSeqNP_001806.2. NM_001815.2.
UniGeneHs.11.

3D structure databases

ProteinModelPortalP40198.
SMRP40198. Positions 34-141.
ModBaseSearch...

Protein-protein interaction databases

STRINGP40198.

PTM databases

PhosphoSiteP40198.

Polymorphism databases

DMDM218511974.

Proteomic databases

PRIDEP40198.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000357396; ENSP00000349971; ENSG00000170956.
GeneID1084.
KEGGhsa:1084.
UCSCuc010eia.1. human.

Organism-specific databases

CTD1084.
GeneCardsGC19P042300.
H-InvDBHIX0202914.
HGNCHGNC:1815. CEACAM3.
HPAHPA011041.
MIM609142. gene.
neXtProtNX_P40198.
PharmGKBPA26359.
GenAtlasSearch...

Phylogenomic databases

eggNOGmaNOG23108.
HOGENOMHBG505999.
HOVERGENHBG007922.
InParanoidP40198.
OMACEPEIHS.
OrthoDBEOG4M0F2K.
PhylomeDBP40198.

Gene expression databases

ArrayExpressP40198.
BgeeP40198.
CleanExHS_CEACAM3.
GenevestigatorP40198.
GermOnlineENSG00000170956. Homo sapiens.

Family and domain databases

InterProIPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KOK06499.
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. False negative.
[Graphical view]
ProtoNetSearch...

Other

SOURCESearch...

Entry information

Entry nameCEAM3_HUMAN
AccessionPrimary (citable) accession number: P40198
Secondary accession number(s): Q3KPH9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 16, 2008
Last modified: January 25, 2012
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families