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Reviewed, UniProtKB/Swiss-Prot P40198 (CEAM3_HUMAN)

Last modified November 4, 2008. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Carcinoembryonic antigen-related cell adhesion molecule 3
Alternative name(s):
    Carcinoembryonic antigen CGM1
    CD66d antigen
Gene names
Name: CEACAM3
Synonyms: CD66D, CGM1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.

Subunit structure

Interacts with S100A9/calprotectin. This interaction is calcium-dependent, but independent of CEACAM3 phosphorylation.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

CGM1a, the predominant CGM1 transcript, is granulocyte-specific. Not detected out of the granulocytic lineage, such as monocytes, lymphocytes, spleen, testis, colon, brain, liver, pancreas, thymus, ovary, placenta, skeletal muscle, prostate, small intestine, heart, lung and kidney.

Domain

The cytosolic domain is involved in S100A9 interaction.

Post-translational modification

Tyrosine-phosphorylated in response to microbial binding. Tyr-230 and Tyr-241 are both required for phosphorylation to be detected.

Sequence similarities

Belongs to the immunoglobulin superfamily. CEA family.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Caution

This is not the ortholog of rat CEACAM3.

Ontologies

Keywords

   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainImmunoglobulin domain
Signal
Transmembrane
   PTMGlycoprotein
Phosphoprotein

Gene Ontology (GO)

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform CGM1A (identifier: P40198-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform CGM1B (identifier: P40198-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-252: QFHVYQENAP...RMDHKAEVAS → HFHVYPLPKP...EPEIHSTTCL
Isoform CGM1C (identifier: P40198-3)

The sequence of this isoform differs from the canonical sequence as follows:
     182-252: TSIQRDLKEQ...RMDHKAEVAS → PWSLPQLCLLDVPSLHCLGPPTQPQDSSFHL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 By similarity
Chain35 – 252218Carcinoembryonic antigen-related cell adhesion molecule 3
PRO_0000014565

Regions

Topological domain35 – 155121Extracellular Potential
Transmembrane156 – 17621 Potential
Topological domain177 – 25276Cytoplasmic Potential
Domain35 – 142108Ig-like V-type

Amino acid modifications

Glycosylation1041N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence137 – 252116QFHVY…AEVAS → HFHVYPLPKPSISSNNSNPK EDKDAVAFTCEPEIHSTTCL in isoform CGM1B.
VSP_002486
Alternative sequence182 – 25271TSIQR…AEVAS → PWSLPQLCLLDVPSLHCLGP PTQPQDSSFHL in isoform CGM1C.
VSP_002487
Natural variant71S → P
VAR_003905

Experimental info

Mutagenesis2301Y → F: Loss of phosphorylation and 30% reduction in bacterial uptake. More than 60% reduction in bacterial uptake and loss of RAC1 stimulation; when associated with F-241
Mutagenesis2411Y → F: Loss of phosphorylation and 30% reduction in bacterial uptake. More than 60% reduction in bacterial uptake and loss of RAC1 stimulation; when associated with F-230
Sequence conflict491K → N Ref.2 Ref.3
Sequence conflict841I → T in BAA14322. Ref.2
Sequence conflict2151S → T in BAA14321. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform CGM1A [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 0C9F0C51C6A433E4

FASTA25227,077
        10         20         30         40         50         60 
MGPPSASPHR ECIPWQGLLL TASLLNFWNP PTTAKLTIES MPLSVAEGKE VLLLVHNLPQ 

        70         80         90        100        110        120 
HLFGYSWYKG ERVDGNSLIV GYVIGTQQAT PGAAYSGRET IYTNASLLIQ NVTQNDIGFY 

       130        140        150        160        170        180 
TLQVIKSDLV NEEATGQFHV YQENAPGLPV GAVAGIVTGV LVGVALVAAL VCFLLLAKTG 

       190        200        210        220        230        240 
RTSIQRDLKE QQPQALAPGR GPSHSSAFSM SPLSSAQAPL PNPRTAASIY EELLKHDTNI 

       250 
YCRMDHKAEV AS 

« Hide

Isoform CGM1B [UniParc].

Checksum: 1D6820975B598CEC
Show »

17619,283
Isoform CGM1C [UniParc].

Checksum: A146AF256C846762
Show »

21222,770

References

[1]"Genomic organization, splice variants and expression of CGM1, a CD66-related member of the carcinoembryonic antigen gene family."
Nagel G., Grunert F., Kuijpers T.W., Watt S.M., Thompson J., Zimmermann W.A.
Eur. J. Biochem. 214:27-35(1993) [PubMed: 8508798] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of nonspecific cross-reacting antigens in human granulocytes."
Kuroki M., Arakawa F., Matsuo Y., Oikawa S., Misumi Y., Nakazato H., Matsuoka Y.
J. Biol. Chem. 266:11810-11817(1991) [PubMed: 2050678] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leukocyte.
[3]"Identification of three new genes and estimation of the size of the carcinoembryonic antigen family."
Khan W.N., Fraengsmyr L., Teglund S., Israelsson A., Bremer K., Hammarstroem S.
Genomics 14:384-390(1992) [PubMed: 1427854] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-141.
[4]"The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes."
Streichert T., Ebrahimnejad A., Ganzer S., Flayeh R., Wagener C., Bruemmer J.
Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed: 11708798] [Abstract]
Cited for: INTERACTION WITH S100A9, TISSUE SPECIFICITY.
[5]"Immunoreceptor tyrosine-based activation motif phosphorylation during engulfment of Neisseria gonorrhoeae by the neutrophil-restricted CEACAM3 (CD66d) receptor."
McCaw S.E., Schneider J., Liao E.H., Zimmermann W., Gray-Owen S.D.
Mol. Microbiol. 49:623-637(2003) [PubMed: 12864848] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF TYR-230 AND TYR-241.
[6]"Granulocyte CEACAM3 is a phagocytic receptor of the innate immune system that mediates recognition and elimination of human-specific pathogens."
Schmitter T., Agerer F., Peterson L., Muenzner P., Hauck C.R.
J. Exp. Med. 199:35-46(2004) [PubMed: 14707113] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TYR-230 AND TYR-241.
+Additional computationally mapped references.

Cross-references

Sequence databases

L00692 mRNA. Translation: AAA51969.1.
L00693 mRNA. Translation: AAA51970.1.
D90277 mRNA. Translation: BAA14321.1.
D90278 mRNA. Translation: BAA14322.1.
PIRC40428.
S33323.
S33324.
UniGeneHs.11

3D structure databases

HSSPHSSP built from PDB template 1L6Z based on UniProtKB Q61353.
SMRP40198. Positions 34-141.
ModBaseSearch...

PTM databases

PhosphoSiteP40198.

Genome annotation databases

EnsemblENSG00000170956. Homo sapiens. [Contig view]

Organism-specific databases

H-InvDBHIX0040150.
HGNCHGNC:1815. CEACAM3.
HPAHPA011041.
MIM609142. gene.
PharmGKBPA26359.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP40198.
HOVERGENP40198.

Gene expression databases

ArrayExpressP40198.
CleanExHS_CEACAM3.
GermOnlineENSG00000170956. Homo sapiens.

Family and domain databases

InterProIPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameCEAM3_HUMAN
AccessionPrimary (citable) accession number: P40198
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 4, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents