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Protein

Interleukin-6 receptor subunit beta

Gene

Il6st

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Does not bind IL6. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition.By similarity

GO - Molecular functioni

  • cytokine receptor activity Source: InterPro
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • interleukin-6-mediated signaling pathway Source: RGD
  • negative regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of smooth muscle cell migration Source: RGD
  • regulation of hormone secretion Source: RGD
  • response to nutrient Source: RGD
  • triglyceride mobilization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-6 receptor subunit beta
Short name:
IL-6 receptor subunit beta
Short name:
IL-6R subunit beta
Short name:
IL-6R-beta
Short name:
IL-6RB
Alternative name(s):
Interleukin-6 signal transducer
Membrane glycoprotein 130
Short name:
gp130
Oncostatin-M receptor subunit alpha
CD_antigen: CD130
Gene namesi
Name:Il6st
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2903. Il6st.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 618596ExtracellularSequence analysisAdd
BLAST
Transmembranei619 – 64022HelicalSequence analysisAdd
BLAST
Topological domaini641 – 918278CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 918896Interleukin-6 receptor subunit betaPRO_0000010901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 54By similarity
Glycosylationi43 – 431N-linked (GlcNAc...)Sequence analysis
Disulfide bondi48 ↔ 103By similarity
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence analysis
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi134 ↔ 144By similarity
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi172 ↔ 181By similarity
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence analysis
Glycosylationi226 – 2261N-linked (GlcNAc...)Sequence analysis
Glycosylationi382 – 3821N-linked (GlcNAc...)Sequence analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi457 ↔ 465By similarity
Glycosylationi477 – 4771N-linked (GlcNAc...)Sequence analysis
Glycosylationi552 – 5521N-linked (GlcNAc...)Sequence analysis
Modified residuei660 – 6601PhosphoserineBy similarity
Modified residuei666 – 6661PhosphoserineBy similarity
Modified residuei781 – 7811PhosphoserineBy similarity
Modified residuei788 – 7881PhosphoserineBy similarity
Modified residuei828 – 8281PhosphoserineBy similarity
Modified residuei838 – 8381PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation of Ser-781 down-regulates cell surface expression.By similarity
Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP40190.
PRIDEiP40190.

Expressioni

Tissue specificityi

Found in hepatocytes, astrocytes, fibroblasts and endothelial cells.1 Publication

Interactioni

Subunit structurei

Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Forms heterodimers composed of LIFR and IL6ST (type I OSM receptor) which are activated by LIF and OSM. Also forms heterodimers composed of OSMR and IL6ST (type II receptor) which are activated by OSM but not by LIF. Interacts with HCK. Interacts with INPP5D/SHIP1.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018877.

Structurei

3D structure databases

ProteinModelPortaliP40190.
SMRiP40190. Positions 24-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 12095Ig-like C2-typeAdd
BLAST
Domaini125 – 21591Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini223 – 323101Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini328 – 41891Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini422 – 51695Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 61295Fibronectin type-III 5PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi309 – 3135WSXWS motif
Motifi650 – 6589Box 1 motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi724 – 75431Ser-richAdd
BLAST

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF1N. Eukaryota.
ENOG410YNQ4. LUCA.
HOGENOMiHOG000015771.
HOVERGENiHBG052119.
InParanoidiP40190.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR003961. FN3_dom.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 5 hits.
PROSITEiPS50853. FN3. 4 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40190-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALRIWLMQ ALLIFLTTES IGQLVEPCGY IYPEFPVVQR GSNFTATCVL
60 70 80 90 100
KEKCLQVYSV NATYIVWKTN HVAVPKEQVT VINRTASSVT FTDVVFQNVQ
110 120 130 140 150
LTCNILSFGQ IEQNVYGITI LSGYPPDIPT NLSCIVNEGK NMLCQLDPGR
160 170 180 190 200
ETYLETNYTL KSEWATEKFP DCRTKHGTSS CMMGYTPIYF VNIEVWVEAE
210 220 230 240 250
NALGNVSSEP INFDPVDKVK PSPPHNLSVT NSEELSSILK LAWVNSGLDS
260 270 280 290 300
ILRLKSDIQY RTKDASTWIQ VPLEDTVSPR TSFTVQDLKP FTEYVFRIRS
310 320 330 340 350
IKENGKGYWS DWSEEASGTT YEDRPSKAPS FWYKVNANHP QEYRSARLIW
360 370 380 390 400
KTLPLSEANG KILDYEVVLT QSKSVSQTYT VNGTELIVNL TNNRYVASLA
410 420 430 440 450
ARNVVGKSPA TVLTIPGSHF KASHPVVDLK AFPKDNLLWV EWTPPSKPVN
460 470 480 490 500
KYILEWCVLS ENSPCIPDWQ QEDGTVNRTH LRGSLLESKC YLITVTPVFP
510 520 530 540 550
GGPGSPESMK AYLKQAAPSK GPTVRTKKVG KNEAVLEWDH LPVDVQNGFI
560 570 580 590 600
RNYSISYRTS VGKEMVVRVD SSHTEYTLSS LSSDTLYMVH MAAYTEEGGK
610 620 630 640 650
DGPEFTFTTL KFAQGEIEAI VVPVCLAFLL TTLLGVLFCF NKRDLIKKHI
660 670 680 690 700
WPNVPDPSKS HIAQWSPHTP PRHNFNSKDQ MYSDANFTDV SVVEIEANNK
710 720 730 740 750
KPCPDDLKSL DLFKKEKIST EGHSSGIGGS SCMSSSRPSI SSSEENESAQ
760 770 780 790 800
STASTVQYST VVHSGYRHQV PSVQVFSRSE STQPLLDSEE RPEDLQLVDS
810 820 830 840 850
VDSGDEILPR QQYFKQSCSQ PGASPDVSHF GRSSQVPSGS EEDFVRLKQQ
860 870 880 890 900
QVSDHISEPY GSEQRRLFQE GSVADALGTG TDGQIERFES VGMETAMDED
910
ISKSYLPQTV RQGGYMPQ
Length:918
Mass (Da):102,450
Last modified:February 1, 1995 - v1
Checksum:i9E18B6FECFF087F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92340 mRNA. No translation available.
PIRiA44257.
UniGeneiRn.12138.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92340 mRNA. No translation available.
PIRiA44257.
UniGeneiRn.12138.

3D structure databases

ProteinModelPortaliP40190.
SMRiP40190. Positions 24-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018877.

Proteomic databases

PaxDbiP40190.
PRIDEiP40190.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi2903. Il6st.

Phylogenomic databases

eggNOGiENOG410IF1N. Eukaryota.
ENOG410YNQ4. LUCA.
HOGENOMiHOG000015771.
HOVERGENiHBG052119.
InParanoidiP40190.

Miscellaneous databases

PROiP40190.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR003961. FN3_dom.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 5 hits.
PROSITEiPS50853. FN3. 4 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the rat liver IL-6 signal transducing molecule, gp130."
    Wang Y., Nesbitt J.E., Fuentes N.L., Fuller G.M.
    Genomics 14:666-672(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-838, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIL6RB_RAT
AccessioniPrimary (citable) accession number: P40190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.