P40187 (PIG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GSY2-interacting protein PIG2

Gene names

Name:	PIG2
Ordered Locus Names:	YIL045W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with glycogen synthase 2 (GSY2); possibly also interacts with phosphatase 1 (GLC7).
Miscellaneous	Present with 996 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of glycogen biosynthetic process Inferred from physical interaction Ref.4. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	protein phosphatase type 1 regulator activity Inferred from sequence or structural similarity Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	GSY2-interacting protein PIG2		PRO_0000071522
Regions
	Domain	384 – 508	125	CBM21
Amino acid modifications
	Modified residue	162	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	196	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	537	1	F → L in AAU09747. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P40187 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 73ED8F5CC6692172	FASTA	538	61,938
	10 20 30 40 50 60 MATTTQPQNI LMDEPLNLPN NSAHNNNYGN INANIRTFAG MSMHMHPARL NSLEFLHKPR 70 80 90 100 110 120 RLSNVKLHRL PQDELQRNTD MNKGMYFNGK QVHAHHPFIN SGANFNAHHQ DVSKLGEEED 130 140 150 160 170 180 EISPLSHDNF QYESEENGNP SPPIYKKSGE LVKSSLKRRS KSLPITPKSI FNKTGSKSKH 190 200 210 220 230 240 VNLDHVDTRL LQRSKSVHFD RVLPIKLFNE NEKPIDVGKQ MVQQDVLNFK HKPLTRLSAL 250 260 270 280 290 300 NGGSDSVPIE DLLSENNQNE YGDTWLQNPK GVFLFGTNSN NRRNKKKKFK LSDDDSDIEN 310 320 330 340 350 360 DNDSDDAINR LVRQQDKDQA HLAHGLKNLL INDDDDYLET RTNSAKSGAN LFIGNSKRIV 370 380 390 400 410 420 GLYNKNFPIL SDRNRKSLKL NIFLNLSRGR PVFLQEITLL TGFHNMVIIG KVFVKNIYFD 430 440 450 460 470 480 KKIIVRYTWD AWRTFHESEC VYFSNANGIL PGSNMDIFKF SIDDIHNPND KDSNISQLEF 490 500 510 520 530 CIQYLTWGVD RSRKEYWDNN DSANYKIDVV TNETRTGPTT DVNDNYEMKH SLFRNPFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p." Cheng C., Huang D., Roach P.J. Yeast 13:1-8(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE, CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162 AND SER-196, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46861 Genomic DNA. Translation: CAA86906.1. AY723830 Genomic DNA. Translation: AAU09747.1. BK006942 Genomic DNA. Translation: DAA08503.1.
PIR	S49933.
RefSeq	NP_012219.3. NM_001179395.3.
3D structure databases
ProteinModelPortal	P40187.
SMR	P40187. Positions 408-507.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5191N.
IntAct	P40187. 11 interactions.
MINT	MINT-537691.
STRING	4932.YIL045W.
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21.
Proteomic databases
PaxDb	P40187.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL045W; YIL045W; YIL045W.
GeneID	854766.
KEGG	sce:YIL038C. sce:YIL045W.
Organism-specific databases
CYGD	YIL045w.
SGD	S000001307. PIG2.
Phylogenomic databases
eggNOG	KOG3986.
GeneTree	ENSGT00530000067694.
KO	K12580.
OMA	INTIEHI.
OrthoDB	EOG45TGXJ.
Gene expression databases
Genevestigator	P40187.
GermOnline	YIL045W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005036. CBM_21. IPR016717. Pase-1_int. [Graphical view]
Pfam	PF03370. CBM_21. 1 hit. [Graphical view]
PIRSF	PIRSF018234. PPase_interacting. 1 hit.
PROSITE	PS51159. CBM21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977520.

Entry information

Entry name

PIG2_YEAST

Accession

Primary (citable) accession number: P40187
Secondary accession number(s): D6VVN7, E9P960

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program