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Protein

PHO85 cyclin-7

Gene

PCL7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Together with cyclin PCL6, controls glycogen phosphorylase and glycogen synthase activities in response to nutrient availablility. The PCL7-PHO85 cyclin-CDK holoenzyme has GLC8 kinase activity and phosphorylates and inactivates the phosphatase PP1-2 inhibitor GLC8, causing activation of PP1-2, which then dephosphorylates and activates glycogen phosphorylase. PCL7-PHO85 also phosphorylates MMR1 and YJL084C.6 Publications

Enzyme regulationi

The PCL7-PHO85 cyclin-CDK is inhibited by PHO81 in low-phosphate conditions.1 Publication

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • glycogen metabolic process Source: UniProtKB-KW
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • regulation of glycogen biosynthetic process Source: SGD
  • regulation of glycogen catabolic process Source: SGD
  • regulation of protein stability Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-31321-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PHO85 cyclin-7
Alternative name(s):
PHO85-associated protein 1
Gene namesi
Name:PCL7
Synonyms:PAP1
Ordered Locus Names:YIL050W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL050W.
SGDiS000001312. PCL7.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 285284PHO85 cyclin-7PRO_0000202991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei69 – 691PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40186.

PTM databases

iPTMnetiP40186.

Expressioni

Inductioni

Cell cycle regulated, with a peak in mid to late S phase.1 Publication

Interactioni

Subunit structurei

Forms a cyclin-CDK complex with PHO85. Interacts with the substrate proteins MMR1 and YJL084C. Interacts with the CDK inhibitor (CKI) PHO81.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO85P171579EBI-25021,EBI-13327

Protein-protein interaction databases

BioGridi34940. 19 interactions.
DIPiDIP-1508N.
IntActiP40186. 5 interactions.
MINTiMINT-406318.

Structurei

3D structure databases

ProteinModelPortaliP40186.
SMRiP40186. Positions 98-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. PHO80 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000066532.
InParanoidiP40186.
OrthoDBiEOG7PP5HV.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSSPSKKT TTSPINIPGG NRDNLIIGPH SHSFKTDPFS SNNSSLLSKI
60 70 80 90 100
STNPSLESPF SSKSLLDCSP VQAVKKSLES EAKTHSLDEE TNEQTDVKIL
110 120 130 140 150
NIADFPTDEL ILMISALLNR IITANDETTD VSQQVSDETE DELLTPILAF
160 170 180 190 200
YGKNVPEIAV VQYLERIQKY CPTTNDIFLS LLVYFDRISK NYGHSSERNG
210 220 230 240 250
CAKQLFVMDS GNIHRLLITG VTICTKFLSD FFYSNSRYAK VGGISLQELN
260 270 280
HLELQFLILC DFKLLVSVEE MQKYANLLYK FWNDQ
Length:285
Mass (Da):32,037
Last modified:January 23, 2007 - v3
Checksum:iF81EEA8E39BC0FC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA. Translation: CAA86172.1.
BK006942 Genomic DNA. Translation: DAA08497.1.
PIRiS48429.
RefSeqiNP_012214.3. NM_001179400.3.

Genome annotation databases

EnsemblFungiiYIL050W; YIL050W; YIL050W.
GeneIDi854761.
KEGGisce:YIL050W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38060 Genomic DNA. Translation: CAA86172.1.
BK006942 Genomic DNA. Translation: DAA08497.1.
PIRiS48429.
RefSeqiNP_012214.3. NM_001179400.3.

3D structure databases

ProteinModelPortaliP40186.
SMRiP40186. Positions 98-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34940. 19 interactions.
DIPiDIP-1508N.
IntActiP40186. 5 interactions.
MINTiMINT-406318.

PTM databases

iPTMnetiP40186.

Proteomic databases

MaxQBiP40186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL050W; YIL050W; YIL050W.
GeneIDi854761.
KEGGisce:YIL050W.

Organism-specific databases

EuPathDBiFungiDB:YIL050W.
SGDiS000001312. PCL7.

Phylogenomic databases

GeneTreeiENSGT00530000066532.
InParanoidiP40186.
OrthoDBiEOG7PP5HV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31321-MONOMER.

Miscellaneous databases

PROiP40186.

Family and domain databases

InterProiIPR013922. Cyclin_PHO80-like.
[Graphical view]
PfamiPF08613. Cyclin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Cloning and expression of a novel PHO85 associated protein PAP1 gene of Saccharomyces cerevisiae."
    Wu J.S., Xia Z.X., Cao Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 30:14-20(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11, INTERACTION WITH PHO85.
  4. "A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase."
    Measday V., Moore L., Retnakaran R., Lee J., Donoviel M., Neiman A.M., Andrews B.J.
    Mol. Cell. Biol. 17:1212-1223(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PHO85.
  5. Cited for: FUNCTION, INTERACTION WITH PHO81 AND PHO85, ENZYME REGULATION, INDUCTION.
  6. "The yeast cyclins Pcl6p and Pcl7p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p."
    Wang Z., Wilson W.A., Fujino M.A., Roach P.J.
    FEBS Lett. 506:277-280(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Phosphorylation of YLR190w by PAP1 PHO85 kinase complex."
    Shi X.Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:187-192(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MMR1, PHOSPHORYLATION OF MMR1.
  8. "Analysis of phosphorylation of YJL084c, a yeast protein."
    Shi X.Z., Ao S.Z.
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:433-438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YJL084C, PHOSPHORYLATION OF YJL084C.
  9. "Pho85 phosphorylates the Glc7 protein phosphatase regulator Glc8 in vivo."
    Tan Y.S.H., Morcos P.A., Cannon J.F.
    J. Biol. Chem. 278:147-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION OF GLC8.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Regulation of yeast glycogen phosphorylase by the cyclin-dependent protein kinase Pho85p."
    Wilson W.A., Wang Z., Roach P.J.
    Biochem. Biophys. Res. Commun. 329:161-167(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPCL7_YEAST
AccessioniPrimary (citable) accession number: P40186
Secondary accession number(s): D6VVN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3390 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.