ID   ZC3HD_YEAST             Reviewed;         298 AA.
AC   P40167; D6W0Z1;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 124.
DE   RecName: Full=Sporulation-specific with a leucine zipper motif protein 1;
GN   Name=SLZ1; OrderedLocusNames=YNL196C; ORFNames=N1386;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=7725799; DOI=10.1002/yea.320101213;
RA   Jonniaux J.-L., Coster F., Purnelle B., Goffeau A.;
RT   "A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries
RT   WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8
RT   new open reading frames of unknown function.";
RL   Yeast 10:1639-1645(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=11921089; DOI=10.1002/yea.843.abs;
RA   Briza P., Bogengruber E., Thuer A., Ruetzler M., Muensterkoetter M.,
RA   Dawes I.W., Breitenbach M.;
RT   "Systematic analysis of sporulation phenotypes in 624 non-lethal homozygous
RT   deletion strains of Saccharomyces cerevisiae.";
RL   Yeast 19:403-422(2002).
RN   [5]
RP   IDENTIFICATION IN THE MIS COMPLEX, AND FUNCTION.
RX   PubMed=22685417; DOI=10.1371/journal.pgen.1002732;
RA   Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.;
RT   "RNA methylation by the MIS complex regulates a cell fate decision in
RT   yeast.";
RL   PLoS Genet. 8:E1002732-E1002732(2012).
RN   [6]
RP   FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, INDUCTION BY IME1, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=24269006; DOI=10.1016/j.cell.2013.10.047;
RA   Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P.,
RA   Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A.,
RA   Lander E.S., Fink G.R., Regev A.;
RT   "High-resolution mapping reveals a conserved, widespread, dynamic mRNA
RT   methylation program in yeast meiosis.";
RL   Cell 155:1409-1421(2013).
RN   [7]
RP   FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, AND INTERACTION WITH VIR1.
RX   PubMed=36930734; DOI=10.1093/genetics/iyad043;
RA   Park Z.M., Belnap E., Remillard M., Rose M.D.;
RT   "Vir1p, the yeast homolog of virilizer, is required for mRNA m6A
RT   methylation and meiosis.";
RL   Genetics 224:1-16(2023).
CC   -!- FUNCTION: Component of the MIS complex, a complex that mediates N6-
CC       methyladenosine (m6A) methylation of meiotic mRNAs and is required for
CC       initiation of meiosis, progression through the meiotic divisions and
CC       sporulation (PubMed:36930734, PubMed:22685417, PubMed:24269006). In the
CC       complex, mediates the entry of the MIS complex in nucleolus, where
CC       methylation takes place (PubMed:24269006).
CC       {ECO:0000269|PubMed:22685417, ECO:0000269|PubMed:24269006,
CC       ECO:0000269|PubMed:36930734}.
CC   -!- SUBUNIT: Component of the MIS (mRNA N6-methyladenosine (m6A)
CC       methylation) complex, at least composed of IME4, KAR4, MUM2, SLZ1, and
CC       VIR1 (PubMed:22685417, PubMed:24269006). Interacts with VIR1
CC       (PubMed:36930734). {ECO:0000269|PubMed:22685417,
CC       ECO:0000269|PubMed:24269006, ECO:0000269|PubMed:36930734}.
CC   -!- INTERACTION:
CC       P40167; P41833: IME4; NbExp=2; IntAct=EBI-29095, EBI-9210;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24269006}. Nucleus,
CC       nucleolus {ECO:0000269|PubMed:24269006}. Note=Mediates localization of
CC       the MIS complex to the nucleolus during the period of m6A mRNA
CC       accumulation. {ECO:0000269|PubMed:24269006}.
CC   -!- INDUCTION: During sporulation. Expression is activated by IME1 during
CC       meiosis. {ECO:0000269|PubMed:11921089, ECO:0000269|PubMed:24269006}.
CC   -!- DISRUPTION PHENOTYPE: Impairs sporulation.
CC       {ECO:0000269|PubMed:11921089}.
CC   -!- SIMILARITY: Belongs to the ZC3H13 family. {ECO:0000305}.
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DR   EMBL; X78898; CAA55512.1; -; Genomic_DNA.
DR   EMBL; Z71472; CAA96091.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10357.1; -; Genomic_DNA.
DR   PIR; S50735; S50735.
DR   RefSeq; NP_014203.1; NM_001183034.1.
DR   AlphaFoldDB; P40167; -.
DR   SMR; P40167; -.
DR   BioGRID; 35637; 58.
DR   ComplexPortal; CPX-3211; MIS complex.
DR   DIP; DIP-2978N; -.
DR   IntAct; P40167; 2.
DR   STRING; 4932.YNL196C; -.
DR   PaxDb; 4932-YNL196C; -.
DR   PeptideAtlas; P40167; -.
DR   EnsemblFungi; YNL196C_mRNA; YNL196C; YNL196C.
DR   GeneID; 855525; -.
DR   KEGG; sce:YNL196C; -.
DR   AGR; SGD:S000005140; -.
DR   SGD; S000005140; SLZ1.
DR   VEuPathDB; FungiDB:YNL196C; -.
DR   HOGENOM; CLU_937518_0_0_1; -.
DR   InParanoid; P40167; -.
DR   OrthoDB; 2025594at2759; -.
DR   BioCyc; YEAST:G3O-33206-MONOMER; -.
DR   BioGRID-ORCS; 855525; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P40167; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P40167; Protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR   GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IDA:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IMP:SGD.
DR   GO; GO:0080009; P:mRNA methylation; IMP:SGD.
DR   GO; GO:2000221; P:negative regulation of pseudohyphal growth; IMP:SGD.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytoplasm; Meiosis; Nucleus; Reference proteome; Sporulation.
FT   CHAIN           1..298
FT                   /note="Sporulation-specific with a leucine zipper motif
FT                   protein 1"
FT                   /id="PRO_0000203397"
FT   REGION          18..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          109..128
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        109..123
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   298 AA;  34318 MW;  72B8AE0DB8D8F1E0 CRC64;
     MMNKVDQIIG YKKYEVKLPK DRQVKKNKSK GGNVDQIDTK REKDKMRAFG EERKKFLDKM
     AKNKKKNTSR KDREKPKEVE KENYKREDKR LKEQKKLSLA KEFRFKEPNS EAINQNTAAE
     NGKPKPQTGL DFDIDHQTVS KIMIDQAIQT SSPLNVQLTE LFNDNVLDVS KDSQFVLQDM
     EFTSWERRWS NCSTTSNATT VSSVPDPKYN INYNDITSFN SVALITEDLN ISASSNGLHE
     RGKKLLQQEM EYSNKVKNVT IGLERLCMDE KPVPDAGITM QQASRWSEFP TCCDQAAI
//