NAD(P)H-hydrate epimerase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40165 (NNRE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NAD(P)H-hydrate epimerase
EC=5.1.99.6

Alternative name(s):
NAD(P)HX epimerase

Gene names

Ordered Locus Names:	YNL200C
ORF Names:	N1370

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Ref.8
Catalytic activity	(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide. HAMAP-Rule MF_03159 (6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.
Cofactor	Binds 1 potassium ion per subunit By similarity.
Subcellular location	Cytoplasm. Mitochondrion Ref.5 Ref.7.
Miscellaneous	Present with 1380 molecules/cell in log phase SD medium. HAMAP-Rule MF_03159
Sequence similarities	Belongs to the nnrE/AIBP family. Contains 1 YjeF N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Ligand	Metal-binding NAD NADP Nucleotide-binding Potassium
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nicotinamide nucleotide metabolic process Inferred from direct assay Ref.8. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay Ref.7 PubMed 16823961. Source: SGD
Molecular_function	NADHX epimerase activity Inferred from direct assay Ref.8. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

NAD(P)H-hydrate epimerase HAMAP-Rule MF_03159

PRO_0000119061

Regions

Domain

12 – 234

223

YjeF N-terminal

Region

69 – 73

NAD(P)HX By similarity

Region

142 – 148

NAD(P)HX By similarity

Sites

Metal binding

Potassium By similarity

Metal binding

138

Potassium By similarity

Metal binding

176

Potassium By similarity

Binding site

173

NAD(P)HX By similarity

Secondary structure

246

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P40165 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 78595E7C92C5E11A
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FASTA

246

27,521

        10         20         30         40         50         60 
MSTLKVVSSK LAAEIDKELM GPQIGFTLQQ LMELAGFSVA QAVCRQFPLR GKTETEKGKH 

        70         80         90        100        110        120 
VFVIAGPGNN GGDGLVCARH LKLFGYNPVV FYPKRSERTE FYKQLVHQLN FFKVPVLSQD 

       130        140        150        160        170        180 
EGNWLEYLKP EKTLCIVDAI FGFSFKPPMR EPFKGIVEEL CKVQNIIPIV SVDVPTGWDV 

       190        200        210        220        230        240 
DKGPISQPSI NPAVLVSLTV PKPCSSHIRE NQTTHYVGGR FIPRDFANKF GFEPFGYEST 


DQILKL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function." Jonniaux J.-L., Coster F., Purnelle B., Goffeau A. Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair." Marbaix A.Y., Noel G., Detroux A.M., Vertommen D., Van Schaftingen E., Linster C.L. J. Biol. Chem. 286:41246-41252(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Crystal structure of yeast hypothetical protein YNU0_yeast." New York structural genomix research consortium (NYSGXRC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X78898 Genomic DNA. Translation: CAA55508.1.
Z71476 Genomic DNA. Translation: CAA96099.1.
AY692583 Genomic DNA. Translation: AAT92602.1.
BK006947 Genomic DNA. Translation: DAA10354.1.

PIR

S50731.

RefSeq

NP_014199.1. NM_001183038.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JZT	X-ray	1.94	A/B	1-246	[»]

ProteinModelPortal

P40165.

SMR

P40165. Positions 4-246.

ModBase

Search...

Protein-protein interaction databases

STRING

4932.YNL200C.

Proteomic databases

PaxDb

P40165.

PeptideAtlas

P40165.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNL200C; YNL200C; YNL200C.

GeneID

855521.

KEGG

sce:YNL200C.

Organism-specific databases

CYGD

YNL200c.

SGD

S000005144. YNL200C.

Phylogenomic databases

eggNOG

COG0062.

GeneTree

ENSGT00390000007227.

HOGENOM

HOG000174236.

OMA

ARHLKMF.

OrthoDB

EOG4RBTTS.

Gene expression databases

Genevestigator

P40165.

GermOnline

YNL200C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.40.50.10260. 1 hit.

HAMAP

MF_01966. NADHX_epimerase.

InterPro

IPR026600. NnrE/AIBP.
IPR004443. YjeF_N_dom.
[Graphical view]

Pfam

PF03853. YjeF_N. 1 hit.
[Graphical view]

SUPFAM

SSF64153. YjeF_N. 1 hit.

TIGRFAMs

TIGR00197. yjeF_nterm. 1 hit.

PROSITE

PS51385. YJEF_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P40165.

NextBio

979553.

Entry information

Entry name

NNRE_YEAST

Accession

Primary (citable) accession number: P40165
Secondary accession number(s): D6W0Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents