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Protein

NAD(P)H-hydrate epimerase

Gene

YNL200C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.UniRule annotation1 Publication

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701PotassiumUniRule annotation
Metal bindingi138 – 1381PotassiumUniRule annotation
Binding sitei173 – 1731NAD(P)HXUniRule annotation
Metal bindingi176 – 1761PotassiumUniRule annotation

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • NADHX epimerase activity Source: SGD
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • nicotinamide nucleotide metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciYEAST:G3O-33209-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimeraseUniRule annotation
Gene namesi
Ordered Locus Names:YNL200C
ORF Names:N1370
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL200C.
SGDiS000005144. YNL200C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246NAD(P)H-hydrate epimerasePRO_0000119061Add
BLAST

Proteomic databases

MaxQBiP40165.
PeptideAtlasiP40165.

Interactioni

Protein-protein interaction databases

BioGridi35634. 15 interactions.
MINTiMINT-4500304.

Structurei

Secondary structure

1
246
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 1911Combined sources
Turni22 – 243Combined sources
Helixi28 – 4619Combined sources
Helixi54 – 574Combined sources
Beta strandi60 – 656Combined sources
Helixi69 – 8315Combined sources
Beta strandi88 – 914Combined sources
Helixi100 – 11112Combined sources
Helixi124 – 1285Combined sources
Turni130 – 1323Combined sources
Beta strandi133 – 1408Combined sources
Helixi153 – 16311Combined sources
Turni164 – 1663Combined sources
Beta strandi169 – 1746Combined sources
Turni180 – 1823Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi193 – 2008Combined sources
Helixi203 – 2075Combined sources
Turni210 – 2123Combined sources
Beta strandi214 – 2185Combined sources
Helixi224 – 2296Combined sources
Beta strandi242 – 2454Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JZTX-ray1.94A/B1-246[»]
ProteinModelPortaliP40165.
SMRiP40165. Positions 4-246.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40165.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 234223YjeF N-terminalUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 735NAD(P)HXUniRule annotation
Regioni142 – 1487NAD(P)HXUniRule annotation

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
InParanoidiP40165.
KOiK17759.
OMAiELMSTGA.
OrthoDBiEOG789CPC.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLKVVSSK LAAEIDKELM GPQIGFTLQQ LMELAGFSVA QAVCRQFPLR
60 70 80 90 100
GKTETEKGKH VFVIAGPGNN GGDGLVCARH LKLFGYNPVV FYPKRSERTE
110 120 130 140 150
FYKQLVHQLN FFKVPVLSQD EGNWLEYLKP EKTLCIVDAI FGFSFKPPMR
160 170 180 190 200
EPFKGIVEEL CKVQNIIPIV SVDVPTGWDV DKGPISQPSI NPAVLVSLTV
210 220 230 240
PKPCSSHIRE NQTTHYVGGR FIPRDFANKF GFEPFGYEST DQILKL
Length:246
Mass (Da):27,521
Last modified:February 1, 1995 - v1
Checksum:i78595E7C92C5E11A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78898 Genomic DNA. Translation: CAA55508.1.
Z71476 Genomic DNA. Translation: CAA96099.1.
AY692583 Genomic DNA. Translation: AAT92602.1.
BK006947 Genomic DNA. Translation: DAA10354.1.
PIRiS50731.
RefSeqiNP_014199.1. NM_001183038.1.

Genome annotation databases

EnsemblFungiiYNL200C; YNL200C; YNL200C.
GeneIDi855521.
KEGGisce:YNL200C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78898 Genomic DNA. Translation: CAA55508.1.
Z71476 Genomic DNA. Translation: CAA96099.1.
AY692583 Genomic DNA. Translation: AAT92602.1.
BK006947 Genomic DNA. Translation: DAA10354.1.
PIRiS50731.
RefSeqiNP_014199.1. NM_001183038.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JZTX-ray1.94A/B1-246[»]
ProteinModelPortaliP40165.
SMRiP40165. Positions 4-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35634. 15 interactions.
MINTiMINT-4500304.

Proteomic databases

MaxQBiP40165.
PeptideAtlasiP40165.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL200C; YNL200C; YNL200C.
GeneIDi855521.
KEGGisce:YNL200C.

Organism-specific databases

EuPathDBiFungiDB:YNL200C.
SGDiS000005144. YNL200C.

Phylogenomic databases

GeneTreeiENSGT00390000007227.
HOGENOMiHOG000174236.
InParanoidiP40165.
KOiK17759.
OMAiELMSTGA.
OrthoDBiEOG789CPC.

Enzyme and pathway databases

BioCyciYEAST:G3O-33209-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40165.
NextBioi979553.
PROiP40165.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function."
    Jonniaux J.-L., Coster F., Purnelle B., Goffeau A.
    Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair."
    Marbaix A.Y., Noel G., Detroux A.M., Vertommen D., Van Schaftingen E., Linster C.L.
    J. Biol. Chem. 286:41246-41252(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Crystal structure of yeast hypothetical protein YNU0_yeast."
    New York structural genomix research consortium (NYSGXRC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS).

Entry informationi

Entry nameiNNRE_YEAST
AccessioniPrimary (citable) accession number: P40165
Secondary accession number(s): D6W0Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.