P40164 (PP4R3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 4 regulatory subunit 3
Short name=PP4R3

Gene names

Name:	PSY2
Ordered Locus Names:	YNL201C
ORF Names:	N1366

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core regulatory subunit of the histone H2A phosphatase complex, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. Ref.7
Subunit structure	Regulatory subunit 3 (R3) of the histone H2A phosphatase complex (HTP-C) consisting of PPH3, PSY2 and PSY4. Interacts with SPT4, SPT5 and TIP41. Ref.6 Ref.7
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 7010 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of DNA damage checkpoint Inferred from mutant phenotype Ref.7PubMed 17517611. Source: SGD protein dephosphorylation Inferred from mutant phenotype PubMed 17517611. Source: SGD response to DNA damage stimulus Inferred from genetic interaction PubMed 15598824. Source: SGD signal transduction involved in meiotic recombination checkpoint Inferred from mutant phenotype PubMed 20951350. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PSY4	P38193	8	EBI-29107,EBI-21239
RRD1	P40454	3	EBI-29107,EBI-25278
SPT5	P27692	6	EBI-29107,EBI-17937

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	Serine/threonine-protein phosphatase 4 regulatory subunit 3		PRO_0000203395

Sequences

	Sequence		Length	Mass (Da)
	P40164 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9AB94C259FF7A55B	FASTA	858	98,053
	10 20 30 40 50 60 MSLPGTPTTS PTPMDEDTEQ AVSVNTEPKR VKVYILENNE WKDTGTGFCI GEVDEGKFAY 70 80 90 100 110 120 LVVSDEDSPT ETLLKSKLEG NIEYQRQEET LIVWKDLGGK DIALSFEESM GCDTLCEFIV 130 140 150 160 170 180 HVQRNIESNI SLVTVKSSDN GLGSVHDIIT GPVTLPSNDQ QQNSQTLLEA LKILNENTSF 190 200 210 220 230 240 DFLKNETIEF ILQSNYIDTL ISHFHKAEEE KIPKDLFLLS NIIKTLILYN KRDILESMVE 250 260 270 280 290 300 DDRIMGIVGI LEYDTEYPTS KANHRKYLGS KGPNFKEVIP LENEDLKIIM KKCFRLQFLK 310 320 330 340 350 360 DVVLVRFLDD HNFNLISEIV MDLETCIIDF LQVGTFLDRL IELYDTKTLP ESSSEKEKFV 370 380 390 400 410 420 QKRKDGIRLL QQCVQMSINL DAVDRSKFYK TLVRKGLFKV LDYAFHMETD SNVRILATDT 430 440 450 460 470 480 IITIIEHDIL LIHNVQNEDS FKRQHKSAPD DKSSHRKYPQ DYSSSTDSKL LLILSTILLS 490 500 510 520 530 540 DRSPGLREQV VQALNTLLHP EGCVGNGEGS YDLMGRSNYE AKNTSEDFPS FSYGLNSDSI 550 560 570 580 590 600 NLNNYHYSSD EMNNLEPESE SEFQVMEYFA NFYNKIAPIL FGPLIKKDIT TEMAEIDGQI 610 620 630 640 650 660 EKVTKDDLLL IHLVKLVSFV CTEHDRVLSR RFILENGILD SVSKLIGGNH MMQLRLTAVR 670 680 690 700 710 720 CIKNLMCLDD KYYHRYMISK NLYAPVFKLF QENIDKNNLA NSCIQDFFRI IITECRAYQS 730 740 750 760 770 780 DGHNRKEKTN GSYDGNGNDV KTNVNNNRTN FTILNKYLVQ TYGDVLRKAT DIPFIQDMLE 790 800 810 820 830 840 TGEENQPDHS SFENSIEGGN DISVNMSTDG FASNHLEDID IKNVKRLHSE IEHFENDPHY 850 SGDQLAFKKS VDQMNAST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function." Jonniaux J.-L., Coster F., Purnelle B., Goffeau A. Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity." Gingras A.-C., Caballero M., Zarske M., Sanchez A., Hazbun T.R., Fields S., Sonenberg N., Hafen E., Raught B., Aebersold R. Mol. Cell. Proteomics 4:1725-1740(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN HTP-C COMPLEX, MASS SPECTROMETRY, INTERACTION WITH RAD53; SPT4; SPT5 AND TIP41.
[7]	"A phosphatase complex that dephosphorylates gamma-H2AX regulates DNA damage checkpoint recovery." Keogh M.-C., Kim J.-A., Downey M., Fillingham J., Chowdhury D., Harrison J.C., Onishi M., Datta N., Galicia S., Emili A., Lieberman J., Shen X., Buratowski S., Haber J.E., Durocher D., Greenblatt J.F., Krogan N.J. Nature 439:497-501(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN HTP-C COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78898 Genomic DNA. Translation: CAA55507.1. Z71477 Genomic DNA. Translation: CAA96100.1. BK006947 Genomic DNA. Translation: DAA10353.1.
PIR	S50730.
RefSeq	NP_014198.1. NM_001183039.1.
3D structure databases
ProteinModelPortal	P40164.
SMR	P40164. Positions 610-691.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1513N.
IntAct	P40164. 21 interactions.
MINT	MINT-384732.
STRING	4932.YNL201C.
Proteomic databases
PaxDb	P40164.
PeptideAtlas	P40164.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL201C; YNL201C; YNL201C.
GeneID	855520.
KEGG	sce:YNL201C.
Organism-specific databases
CYGD	YNL201c.
SGD	S000005145. PSY2.
Phylogenomic databases
eggNOG	NOG314899.
GeneTree	ENSGT00390000018199.
HOGENOM	HOG000115652.
OMA	TSKANHR.
OrthoDB	EOG4XSPZT.
Gene expression databases
Genevestigator	P40164.
GermOnline	YNL201C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006887. DUF625. [Graphical view]
Pfam	PF04802. SMK-1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979550.

Entry information

Entry name

PP4R3_YEAST

Accession

Primary (citable) accession number: P40164
Secondary accession number(s): D6W0Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program