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Protein

Serine/threonine-protein kinase RIO2

Gene

RIO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the final endonucleolytic cleavage of 20S pre-rRNA at site D in the cytoplasm, converting it into the mature 18S rRNA. Involved in normal export of the pre-40S particles from the nucleus to the cytoplasm. No longer associates with pre-40S subunits in RPS19 disruptions, suggesting it acts after the ribosomal protein in 18S rRNA maturation.5 Publications

Miscellaneous

Present with 10300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123ATPBy similarity1
Active sitei229Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • positive regulation of RNA import into nucleus Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processRibosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33213-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO2 (EC:2.7.11.11 Publication)
Gene namesi
Name:RIO2
Ordered Locus Names:YNL207W
ORF Names:N1342
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL207W
SGDiS000005151 RIO2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73Y → H in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with V-186, H-210, D-364, G-371 and G-417. 1 Publication1
Mutagenesisi105K → I: No change in activity. 1 Publication1
Mutagenesisi186I → V in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, H-210, D-364, G-371 and G-417. 1 Publication1
Mutagenesisi210Y → H in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, D-364, G-371 and G-417. 1 Publication1
Mutagenesisi227H → F: No change in activity. 1 Publication1
Mutagenesisi229D → A: Decrease in activity. 1 Publication1
Mutagenesisi364E → D in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, G-371 and G-417. 1 Publication1
Mutagenesisi371D → G in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-417. 1 Publication1
Mutagenesisi417D → G in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-371. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135321 – 425Serine/threonine-protein kinase RIO2Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei346PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40160
PaxDbiP40160
PRIDEiP40160

PTM databases

iPTMnetiP40160

Interactioni

Subunit structurei

Component of a late pre-40S ribosomal particle, composed of the 40S ribosomal proteins and the non-ribosomal factors DIM1, DIM2, ENP1, HRR25, LTV1, NOB1, RIO2 and TSR1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35629209 interactors.
DIPiDIP-4894N
IntActiP40160 52 interactors.
MINTiP40160
STRINGi4932.YNL207W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6EMLelectron microscopy3.60r1-425[»]
6FAIelectron microscopy3.40l1-425[»]
ProteinModelPortaliP40160
SMRiP40160
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 257Protein kinaseAdd BLAST163

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000003255
HOGENOMiHOG000225685
InParanoidiP40160
KOiK07179
OMAiHREGYTN
OrthoDBiEOG092C4IY4

Family and domain databases

CDDicd05144 RIO2_C, 1 hit
Gene3Di1.10.10.101 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR030484 Rio2
IPR015285 RIO2_wHTH_N
IPR000687 RIO_kinase
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF09202 Rio2_N, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF56112 SSF56112, 1 hit

Sequencei

Sequence statusi: Complete.

P40160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDTSHMRY LTTDDFRVLQ AVEQGSRSHE VVPTPLIHQI SGMRSQSGTN
60 70 80 90 100
RAISDLAKLS LISKMRNVKY DGYRLTYNGI DYLALKTMLN RDTVYSVGNT
110 120 130 140 150
IGVGKESDIY KVSDKNGNPR VMKIHRLGRT SFHSVRNNRD YLKKSNQGAN
160 170 180 190 200
WMHLSRLAAN KEYQFMSMLY SKGFKVPEPF DNSRHIVVME LIEGYPMRRL
210 220 230 240 250
RKHKNIPKLY SDLMCFIVDL ANSGLIHCDF NEFNIMIKDK LEDENDCGFV
260 270 280 290 300
VIDFPQCISI QHQDADYYFQ RDVDCIRRFF KKKLKYEPKP DSSMLDTEGF
310 320 330 340 350
GDGYKYAYPD FKRDVKRTDN LDELVQASGF SKKHPGDRGL ETAVESMRNA
360 370 380 390 400
VYNSDDDMSN DEAEEENGEG DYSEEDEYYD SELDNESSED DSEDAQEEEN
410 420
ERIIEALSSG VENLKMDKLG NYILE
Length:425
Mass (Da):49,125
Last modified:February 1, 1995 - v1
Checksum:i02117D0328118E8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78898 Genomic DNA Translation: CAA55501.1
Z71483 Genomic DNA Translation: CAA96109.1
BK006947 Genomic DNA Translation: DAA10349.1
PIRiS50724
RefSeqiNP_014192.1, NM_001183045.1

Genome annotation databases

EnsemblFungiiYNL207W; YNL207W; YNL207W
GeneIDi855514
KEGGisce:YNL207W

Similar proteinsi

Entry informationi

Entry nameiRIO2_YEAST
AccessioniPrimary (citable) accession number: P40160
Secondary accession number(s): D6W0Y3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 25, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome