P40154 (IES2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ino eighty subunit 2

Gene names

Name:	IES2
Ordered Locus Names:	YNL215W
ORF Names:	N1315

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.
Subunit structure	Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Ref.6
Subcellular location	Nucleus Ref.4.
Miscellaneous	Present with 1470 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the IES2 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Ino eighty subunit 2		PRO_0000084154
Amino acid modifications
	Modified residue	67	1	Phosphoserine Ref.9
	Modified residue	129	1	Phosphoserine Ref.8
	Modified residue	167	1	Phosphoserine Ref.9
	Modified residue	278	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P40154 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6F0AA04387A3B3E8	FASTA	320	36,154
	10 20 30 40 50 60 MDSEASDIEA ELSDSVSAGG EEYIDDDDYT EDIDDQIVTA KSSRRTARRS VPKGVRTSKR 70 80 90 100 110 120 IRDKELSVEV DEDYDEEEDV LSPSKKRHLH TRSMDKRQVA ATASEKSDIG DSKGNDGEIE 130 140 150 160 170 180 DGILEEEESL EKELNRGGGK EVEKSEESYY AQNDVGQKGE EEQDGESGGY EDNEPSISKE 190 200 210 220 230 240 SDELVSVVNG NGNEEDDEVE ATKENTTDST RSTTTRSKML LDLLEDGGSK KKLTDEEIQL 250 260 270 280 290 300 RRAENARKRK NLSEKRLEEE KQDTINKLLK KRAGKSRSHL PNDDEKNDGS SSFVKPRRPY 310 320 NSEGMTRILR RYEEDLFCTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 13.5 kb DNA segment from the left arm of yeast chromosome XIV reveals MER1; RAP1; a new putative member of the DNA replication complex and a new putative serine/threonine phosphatase gene." Coster F., van Dyck L., Jonniaux J.-L., Purnelle B., Goffeau A. Yeast 11:85-91(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Involvement of actin-related proteins in ATP-dependent chromatin remodeling." Shen X., Ranallo R., Choi E., Wu C. Mol. Cell 12:147-155(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67 AND SER-167, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78898 Genomic DNA. Translation: CAA55493.1. Z71491 Genomic DNA. Translation: CAA96117.1. BK006947 Genomic DNA. Translation: DAA10341.1.
PIR	S50716.
RefSeq	NP_014184.1. NM_001183053.1.
3D structure databases
ProteinModelPortal	P40154.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5062N.
IntAct	P40154. 4 interactions.
MINT	MINT-560359.
STRING	4932.YNL215W.
Proteomic databases
PaxDb	P40154.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL215W; YNL215W; YNL215W.
GeneID	855506.
KEGG	sce:YNL215W.
Organism-specific databases
CYGD	YNL215w.
SGD	S000005159. IES2.
Phylogenomic databases
eggNOG	NOG75630.
KO	K11676.
OrthoDB	EOG4BP4NK.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33221-MONOMER.
Gene expression databases
Genevestigator	P40154.
GermOnline	YNL215W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006880. PAPA1. [Graphical view]
Pfam	PF04795. PAPA-1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979514.

Entry information

Entry name

IES2_YEAST

Accession

Primary (citable) accession number: P40154
Secondary accession number(s): D6W0X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program