Reviewed, UniProtKB/Swiss-Prot P40146 (ADCY8_RAT)

Names and origin

Protein names

Recommended name:
    Adenylate cyclase type 8
    EC=4.6.1.1
Alternative name(s):
    Adenylate cyclase type VIII
    ATP pyrophosphate-lyase 8
    Adenylyl cyclase 8
    Ca(2+)/calmodulin-activated adenylyl cyclase

Gene names

Name:

Adcy8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence. Ref.1
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Enzyme regulation	Activated by calcium/calmodulin.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Brain.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from mutant phenotype. Source: RGD cAMP-mediated signaling Ref.1 Inferred from direct assay. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium- and calmodulin-responsive adenylate cyclase activity Ref.1 Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1248	1248	Adenylate cyclase type 8		PRO_0000195707
Regions
	Topological domain	1 – 179	179	Cytoplasmic Potential
	Transmembrane	180 – 200	21	Potential
	Transmembrane	209 – 229	21	Potential
	Transmembrane	244 – 264	21	Potential
	Transmembrane	271 – 291	21	Potential
	Transmembrane	293 – 313	21	Potential
	Transmembrane	318 – 338	21	Potential
	Topological domain	339 – 712	374	Cytoplasmic Potential
	Transmembrane	713 – 733	21	Potential
	Transmembrane	735 – 755	21	Potential
	Transmembrane	784 – 804	21	Potential
	Transmembrane	828 – 848	21	Potential
	Transmembrane	858 – 878	21	Potential
	Transmembrane	891 – 911	21	Potential
	Topological domain	912 – 1248	337	Cytoplasmic Potential
Sites
	Metal binding	416	1	Magnesium 1 By similarity
	Metal binding	416	1	Magnesium 2 By similarity
	Metal binding	417	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	460	1	Magnesium 1 By similarity
	Metal binding	460	1	Magnesium 2 By similarity
Amino acid modifications
	Glycosylation	814	1	N-linked (GlcNAc...) Potential
	Glycosylation	818	1	N-linked (GlcNAc...) Potential
	Glycosylation	885	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P40146-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 0171A3CEED034961	FASTA	1,248	139,823
	10 20 30 40 50 60 MELSDVHCLS GSEELYTIHP TPPAADGGSG SRPQRLLWQT AVRHITEQRF IHGHRGGGGG 70 80 90 100 110 120 GSRKASNPAG SGPNHHAPQL SSDSVLPLYS LGSGERAHNT GGTKVFPERS GSGSASGSGG 130 140 150 160 170 180 GGDLGFLHLD CAPSNSDFFL NGGYSYRGVI FPTLRNSFKS RDLERLYQRY FLGQRRKSEV 190 200 210 220 230 240 VMNVLDVLTK LTLLVLHLSL ASAPMDPLKG ILLGFFTGIE VVICALVVVR KDTTSHTYLQ 250 260 270 280 290 300 YSGVVTWVAM TTQILAAGLG YGLLGDGIGY VLFTLFATYS MLPLPLTWAI LAGLGTSLLQ 310 320 330 340 350 360 VTLQVLIPRL AVFSINQVLA QVVLFMCMNT AGIFISYLSD RAQRQAFLET RRCVEARLRL 370 380 390 400 410 420 ETENQRQERL VLSVLPRFVV LEMINDMTNV EDEHLQHQFH RIYIHRYENV SILFADVKGF 430 440 450 460 470 480 TNLSTTLSAQ ELVRMLNELF ARFDRLAHEH HCLRIKILGD CYYCVSGLPE PRQDHAHCCV 490 500 510 520 530 540 EMGLSMIKTI RFVRSRTKHD VDMRIGIHSG SVLCGVLGLR KWQFDVWSWD VDIANKLESG 550 560 570 580 590 600 GIPGRIHISK ATLDCLSGDY NVEEGHGKER NEFLRKHNIE TYLIKQPEES LLSLPEDIVK 610 620 630 640 650 660 ESVSCSDRRN SGATFTEGSW SPELPFDNIV GKQNTLAALT RNSINLLPNH LAQALHVQSG 670 680 690 700 710 720 PEEINKRIEH TIDLRSGDKL RREHIKPFSL MFKDSSLEHK YSQMRDEVFK SNLVCAFIVL 730 740 750 760 770 780 LFITAIQSLL PSSRLMPMTI QFSILIMLHS ALVLITTAED YKCLPLILRK TCCWINETYL 790 800 810 820 830 840 ARNVIIFASI LINFLGAVIN ILWCDFDKSI PLKNLTFNSS AVFTDICSYP EYFVFTGVLA 850 860 870 880 890 900 MVTCAVFLRL NSVLKLAVLL IMIAIYALLT ETIYAGLFLS YDNLNHSGED FLGTKEASLL 910 920 930 940 950 960 LMAMFLLAVF YHGQQLEYTA RLDFLWRVQA KEEINEMKDL REHNENMLRN ILPGHVARHF 970 980 990 1000 1010 1020 LEKDRDNEEL YSQSYDAVGV MFASIPGFAD FYSQTEMNNQ GVECLRLLNE IIADFDELLG 1030 1040 1050 1060 1070 1080 EDRFQDIEKI KTIGSTYMAV SGLSPEKQQC EDKWGHLCAL ADFSLALTES IQEINKHSFN 1090 1100 1110 1120 1130 1140 NFELRIGISH GSVVAGVIGA KKPQYDIWGK TVNLASRMDS TGVSGRIQVP EETYLILKDQ 1150 1160 1170 1180 1190 1200 GFAFDYRGEI YVKGISEQEG KIKTYFLLGR VQPNPFILPP RRLPGQYSLA AVVLGLVQSL 1210 1220 1230 1240 NRQRQKQLLN ENSNSGIIKS HYNRRTLLTP SGPEPGAQAE GTDKSDLP

References

[1]	"Type VIII adenylyl cyclase. A Ca2+/calmodulin-stimulated enzyme expressed in discrete regions of rat brain." Cali J.J., Zwaagstra J.C., Mons N., Cooper D.M., Krupinski J. J. Biol. Chem. 269:12190-12195(1994) [PubMed: 8163524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26986 mRNA. Translation: AAA20504.1.
IPI	IPI00214981.
PIR	A53588.
RefSeq	NP_058838.1.
UniGene	Rn.10382
3D structure databases
HSSP	HSSP built from PDB template 1CUL based on UniProtKB P30803.
ModBase	Search...
Protein-protein interaction databases
STRING	P40146.
Proteomic databases
PRIDE	P40146.
Genome annotation databases
Ensembl	ENSRNOT00000006789; ENSRNOP00000006789; ENSRNOG00000004890; Rattus norvegicus. [Genome view]
GeneID	29241.
KEGG	rno:29241.
UCSC	NM_017142. rat.
Organism-specific databases
CTD	29241.
RGD	2036. Adcy8.
Phylogenomic databases
HOVERGEN	P40146.
Enzyme and pathway databases
BRENDA	4.6.1.1. 248.
Gene expression databases
ArrayExpress	P40146.
Genevestigator	P40146.
GermOnline	ENSRNOG00000004890. Rattus norvegicus.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. IPR009398. Aden_cycl-like. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF06327. DUF1053. 1 hit. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM00044. CYCc. 2 hits. [Graphical view]
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 2 hits. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608514.

Entry information

Entry name

ADCY8_RAT

Accession

Primary (citable) accession number: P40146

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	October 13, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)