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Protein

Macrophage-capping protein

Gene

CAPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.

GO - Molecular functioni

  • actin filament binding Source: InterPro
  • cadherin binding Source: BHF-UCL
  • protein complex binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • barbed-end actin filament capping Source: ProtInc
  • protein complex assembly Source: ProtInc

Keywordsi

Molecular functionActin capping, Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage-capping protein
Alternative name(s):
Actin regulatory protein CAP-G
Gene namesi
Name:CAPG
Synonyms:AFCP, MCP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000042493.15.
HGNCiHGNC:1474. CAPG.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi822.
OpenTargetsiENSG00000042493.
PharmGKBiPA26056.

Polymorphism and mutation databases

BioMutaiCAPG.
DMDMi313104088.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187531 – 348Macrophage-capping proteinAdd BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei337PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP40121.
PaxDbiP40121.
PeptideAtlasiP40121.
PRIDEiP40121.
TopDownProteomicsiP40121-1. [P40121-1]

2D gel databases

REPRODUCTION-2DPAGEiIPI00027341.

PTM databases

iPTMnetiP40121.
PhosphoSitePlusiP40121.

Expressioni

Tissue specificityi

Macrophages and macrophage-like cells.

Gene expression databases

BgeeiENSG00000042493.
CleanExiHS_CAPG.
ExpressionAtlasiP40121. baseline and differential.
GenevisibleiP40121. HS.

Organism-specific databases

HPAiHPA018843.
HPA019080.
HPA019092.

Interactioni

Subunit structurei

Interacts with NUP62 (PubMed:18266911). Interacts with NUTF2 and RAN; involved in CAPG nuclear import (PubMed:18266911).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SIAH1Q8IUQ43EBI-4291044,EBI-747107

GO - Molecular functioni

  • actin filament binding Source: InterPro
  • cadherin binding Source: BHF-UCL
  • protein complex binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi107272. 35 interactors.
DIPiDIP-61547N.
IntActiP40121. 6 interactors.
MINTiMINT-5232538.
STRINGi9606.ENSP00000263867.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi17 – 26Combined sources10
Beta strandi28 – 33Combined sources6
Turni36 – 40Combined sources5
Beta strandi41 – 43Combined sources3
Beta strandi46 – 53Combined sources8
Beta strandi55 – 57Combined sources3
Beta strandi59 – 65Combined sources7
Helixi71 – 82Combined sources12
Beta strandi92 – 98Combined sources7
Helixi104 – 107Combined sources4
Beta strandi115 – 117Combined sources3
Beta strandi139 – 143Combined sources5
Beta strandi145 – 147Combined sources3
Beta strandi149 – 153Combined sources5
Helixi157 – 159Combined sources3
Beta strandi164 – 170Combined sources7
Beta strandi173 – 178Combined sources6
Helixi184 – 198Combined sources15
Turni200 – 204Combined sources5
Beta strandi207 – 212Combined sources6
Helixi218 – 224Combined sources7
Beta strandi242 – 246Combined sources5
Beta strandi250 – 255Combined sources6
Beta strandi262 – 267Combined sources6
Beta strandi269 – 272Combined sources4
Helixi274 – 276Combined sources3
Beta strandi281 – 286Combined sources6
Helixi288 – 290Combined sources3
Beta strandi291 – 297Combined sources7
Helixi303 – 320Combined sources18
Beta strandi327 – 332Combined sources6
Turni338 – 340Combined sources3
Beta strandi343 – 345Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J72X-ray2.50A1-348[»]
1JHWX-ray2.80A1-348[»]
ProteinModelPortaliP40121.
SMRiP40121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40121.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 75Gelsolin-like 1Add BLAST49
Repeati148 – 188Gelsolin-like 2Add BLAST41
Repeati261 – 307Gelsolin-like 3Add BLAST47

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 146Nuclear localization signalSequence analysis10

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0443. Eukaryota.
ENOG410XR0A. LUCA.
GeneTreeiENSGT00760000119111.
HOGENOMiHOG000235030.
HOVERGENiHBG001093.
InParanoidiP40121.
KOiK10368.
OMAiHNGPEEV.
OrthoDBiEOG091G05SC.
PhylomeDBiP40121.
TreeFamiTF313468.

Family and domain databases

Gene3Di3.40.20.10. 3 hits.
InterProiView protein in InterPro
IPR029006. ADF-H/Gelsolin-like_dom_sf.
IPR029917. CapG.
IPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF13. PTHR11977:SF13. 1 hit.
PfamiView protein in Pfam
PF00626. Gelsolin. 3 hits.
PRINTSiPR00597. GELSOLIN.
SMARTiView protein in SMART
SM00262. GEL. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P40121-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYTAIPQSGS PFPGSVQDPG LHVWRVEKLK PVPVAQENQG VFFSGDSYLV
60 70 80 90 100
LHNGPEEVSH LHLWIGQQSS RDEQGACAVL AVHLNTLLGE RPVQHREVQG
110 120 130 140 150
NESDLFMSYF PRGLKYQEGG VESAFHKTST GAPAAIKKLY QVKGKKNIRA
160 170 180 190 200
TERALNWDSF NTGDCFILDL GQNIFAWCGG KSNILERNKA RDLALAIRDS
210 220 230 240 250
ERQGKAQVEI VTDGEEPAEM IQVLGPKPAL KEGNPEEDLT ADKANAQAAA
260 270 280 290 300
LYKVSDATGQ MNLTKVADSS PFALELLISD DCFVLDNGLC GKIYIWKGRK
310 320 330 340
ANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGHESPIF KQFFKDWK
Length:348
Mass (Da):38,499
Last modified:November 30, 2010 - v2
Checksum:iBE6E5641D15E9C07
GO
Isoform 2 (identifier: P40121-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     207-221: Missing.

Note: No experimental confirmation available.
Show »
Length:333
Mass (Da):36,857
Checksum:iD0CFFAF6C1F8147F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04777641V → I. Corresponds to variant dbSNP:rs2229668Ensembl.1
Natural variantiVAR_047777198R → W. Corresponds to variant dbSNP:rs11539103Ensembl.1
Natural variantiVAR_047778335H → RCombined sources5 PublicationsCorresponds to variant dbSNP:rs6886Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045538207 – 221Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94345 mRNA. Translation: AAA59570.1.
AK225374 mRNA. No translation available.
AC062037 Genomic DNA. Translation: AAY24128.1.
CH471053 Genomic DNA. Translation: EAW99517.1.
CH471053 Genomic DNA. Translation: EAW99518.1.
CH471053 Genomic DNA. Translation: EAW99519.1.
CH471053 Genomic DNA. Translation: EAW99520.1.
BC000728 mRNA. Translation: AAH00728.1.
BC014549 mRNA. Translation: AAH14549.1.
U12026 Genomic DNA. Translation: AAA92670.1.
CCDSiCCDS1974.1. [P40121-1]
CCDS58715.1. [P40121-2]
PIRiA43358.
RefSeqiNP_001243068.1. NM_001256139.1. [P40121-1]
NP_001243069.1. NM_001256140.1. [P40121-2]
NP_001307661.1. NM_001320732.1. [P40121-1]
NP_001307662.1. NM_001320733.1. [P40121-1]
NP_001738.2. NM_001747.3. [P40121-1]
XP_011531424.1. XM_011533122.1. [P40121-1]
XP_011531425.1. XM_011533123.1. [P40121-1]
UniGeneiHs.516155.

Genome annotation databases

EnsembliENST00000263867; ENSP00000263867; ENSG00000042493. [P40121-1]
ENST00000409670; ENSP00000386315; ENSG00000042493. [P40121-1]
ENST00000409724; ENSP00000386965; ENSG00000042493. [P40121-1]
ENST00000409921; ENSP00000387063; ENSG00000042493. [P40121-2]
GeneIDi822.
KEGGihsa:822.
UCSCiuc002spm.3. human. [P40121-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCAPG_HUMAN
AccessioniPrimary (citable) accession number: P40121
Secondary accession number(s): B8ZZS7, D6W5K8, Q53SA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 30, 2010
Last modified: November 22, 2017
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

This protein was originally thought to be a DNA-binding protein with a helix-loop-helix domain.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families