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P40114

- TOP1_BACAN

UniProt

P40114 - TOP1_BACAN

Protein

DNA topoisomerase 1

Gene

topX

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei35 – 351Interaction with DNAPROSITE-ProRule annotation
    Sitei153 – 1531Interaction with DNAPROSITE-ProRule annotation
    Sitei157 – 1571Interaction with DNAPROSITE-ProRule annotation
    Active sitei299 – 2991O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
    Sitei301 – 3011Interaction with DNAPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri603 – 62725C4-type 1Add
    BLAST
    Zinc fingeri693 – 71725C4-type 2Add
    BLAST
    Zinc fingeri784 – 80724C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA topological change Source: InterPro
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Biological processi

    Virulence

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciANTHRA:GBAA_PXO1_0213-MONOMER.
    BANT261594:GJ7F-5791-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topX
    Synonyms:top1
    Ordered Locus Names:pXO1-142, BXA0213, GBAA_pXO1_0213
    Encoded oniPlasmid pXO10 Publication
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000594: Plasmid pXO1

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 870870DNA topoisomerase 1PRO_0000145139Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi261594.GBAA_pXO1_0213.

    Structurei

    3D structure databases

    ProteinModelPortaliP40114.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 128128ToprimPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 1856Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri603 – 62725C4-type 1Add
    BLAST
    Zinc fingeri693 – 71725C4-type 2Add
    BLAST
    Zinc fingeri784 – 80724C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0550.
    HOGENOMiHOG000156583.
    OMAiNWRSETH.
    OrthoDBiEOG60CWM6.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13342. Toprim_Crpt. 2 hits.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40114-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSPRFRHSQ KYIGSKPYYG YYENDHYIVS WCRGHLLELK NPEEMDPKYK    50
    LFQLEHLPLI FQPSYKVIQE NAEQLQILVK LLQRPDVDHA VNICDADREG 100
    ELIYREVYEY AGVNKKQSRV YKSSFEAAEL EAALNRLESA SKYDGLAYSA 150
    KARQYLDYLL GMNITRGCTT KLAQNKFLLS SGRVQMCLLH EIRQRELAIE 200
    NYREQSYYHL QLITDLGLKP VMKTEDQVLN PSPLKSLGEN LKDQYLTVED 250
    FKEGTRKQNP KLLYNLTDLY KDAHAQLQIN AETAKKHIQN LYEEGFITYP 300
    RSSSRHLPTE QVDRVKGVMQ ALAKSRYSLL VQSVDIDAID IKHKTFDDDL 350
    VSSHFAIIPT TKQYQEEGRP EIEKQLYSLV VKRFVGNFMR PAVYLVRDVS 400
    LIDAMGNTYQ IKESVLREKG FLEVFQEEVK EESVETFKVP ILQKGQELQI 450
    YDFELQESKT KKPALHTESS ILTFMETAGR KIDDEHLKEL MKGKRIGTVA 500
    TEAAFIPVLH EKNFIDIEKG KIITTPIGRA FIEQFPVQQI KDPLYTAEME 550
    GMIHRIEKNE MSYENFIAQT NAFVQKITQE IIRIPDTVSY NLIETWKKQI 600
    EVCQCPCGNG IILDRGKFFG CSNHPNCNKG LPKRVKEKTI PTAQVKKLFE 650
    ENKTDIIKGF KSNGKEFSAY LAFVNGEVSF NLPSVEELSL GQCPKCQKGK 700
    ILNRKTFFGC SEYQNGCDFM LPAKIKGKKL SDSQIKKLVN NHVTDFINGF 750
    SGEKGEFTAA IRLKTDLSIC FEFPTTDDRT VGKCPLCQSR VIIGKTNYLC 800
    EQYKRGCDFI VSGMILEKRI TASQIKKLLE KNMTDTVKGF VSKKTGKSFD 850
    AKLTYDSTQK RVTFIYEKKK 870
    Length:870
    Mass (Da):100,092
    Last modified:July 5, 2004 - v2
    Checksum:iCA5E6F00219AB9D4
    GO

    Sequence cautioni

    The sequence AAA22859.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAD32445.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence AAM26155.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97227 Genomic DNA. Translation: AAA22859.1. Different initiation.
    AF065404 Genomic DNA. Translation: AAD32445.1. Different initiation.
    AE011190 Genomic DNA. Translation: AAM26155.2. Different initiation.
    AE017336 Genomic DNA. Translation: AAT35502.1.
    PIRiS41543.
    RefSeqiNP_052837.1. NC_001496.1.
    WP_000520727.1. NC_001496.1.
    YP_022474.1. NC_007322.2.

    Genome annotation databases

    EnsemblBacteriaiAAT35502; AAT35502; GBAA_pXO1_0213.
    GeneIDi2820197.
    3361898.
    KEGGibar:GBAA_pXO1_0213.
    PATRICi32583669. VBIBacAnt112616_0199.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97227 Genomic DNA. Translation: AAA22859.1 . Different initiation.
    AF065404 Genomic DNA. Translation: AAD32445.1 . Different initiation.
    AE011190 Genomic DNA. Translation: AAM26155.2 . Different initiation.
    AE017336 Genomic DNA. Translation: AAT35502.1 .
    PIRi S41543.
    RefSeqi NP_052837.1. NC_001496.1.
    WP_000520727.1. NC_001496.1.
    YP_022474.1. NC_007322.2.

    3D structure databases

    ProteinModelPortali P40114.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 261594.GBAA_pXO1_0213.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT35502 ; AAT35502 ; GBAA_pXO1_0213 .
    GeneIDi 2820197.
    3361898.
    KEGGi bar:GBAA_pXO1_0213.
    PATRICi 32583669. VBIBacAnt112616_0199.

    Phylogenomic databases

    eggNOGi COG0550.
    HOGENOMi HOG000156583.
    OMAi NWRSETH.
    OrthoDBi EOG60CWM6.

    Enzyme and pathway databases

    BioCyci ANTHRA:GBAA_PXO1_0213-MONOMER.
    BANT261594:GJ7F-5791-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13342. Toprim_Crpt. 2 hits.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bacillus anthracis pXO1 virulence plasmid encodes a type 1 DNA topoisomerase."
      Fouet A., Sirard J.-C., Mock M.
      Mol. Microbiol. 11:471-479(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 7702.
    2. "Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes."
      Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P., Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y., Ricke D., Svensson R., Jackson P.J.
      J. Bacteriol. 181:6509-6515(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.
    3. "Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis."
      Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L., Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P., Fraser C.M.
      Science 296:2028-2033(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Ames / isolate Florida / A2012.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.

    Entry informationi

    Entry nameiTOP1_BACAN
    AccessioniPrimary (citable) accession number: P40114
    Secondary accession number(s): Q8KYH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3