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P40114

- TOP1_BACAN

UniProt

P40114 - TOP1_BACAN

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Protein

DNA topoisomerase 1

Gene

topX

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei35 – 351Interaction with DNAPROSITE-ProRule annotation
Sitei153 – 1531Interaction with DNAPROSITE-ProRule annotation
Sitei157 – 1571Interaction with DNAPROSITE-ProRule annotation
Active sitei299 – 2991O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
Sitei301 – 3011Interaction with DNAPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri603 – 62725C4-type 1Add
BLAST
Zinc fingeri693 – 71725C4-type 2Add
BLAST
Zinc fingeri784 – 80724C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA topological change Source: InterPro
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Biological processi

Virulence

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciANTHRA:GBAA_PXO1_0213-MONOMER.
BANT261594:GJ7F-5791-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topX
Synonyms:top1
Ordered Locus Names:pXO1-142, BXA0213, GBAA_pXO1_0213
Encoded oniPlasmid pXO10 Publication
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000000594: Plasmid pXO1

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870DNA topoisomerase 1PRO_0000145139Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi261594.GBAA_pXO1_0213.

Structurei

3D structure databases

ProteinModelPortaliP40114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 128128ToprimPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 1856Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri603 – 62725C4-type 1Add
BLAST
Zinc fingeri693 – 71725C4-type 2Add
BLAST
Zinc fingeri784 – 80724C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0550.
HOGENOMiHOG000156583.
OMAiNWRSETH.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13342. Toprim_Crpt. 2 hits.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40114-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSPRFRHSQ KYIGSKPYYG YYENDHYIVS WCRGHLLELK NPEEMDPKYK
60 70 80 90 100
LFQLEHLPLI FQPSYKVIQE NAEQLQILVK LLQRPDVDHA VNICDADREG
110 120 130 140 150
ELIYREVYEY AGVNKKQSRV YKSSFEAAEL EAALNRLESA SKYDGLAYSA
160 170 180 190 200
KARQYLDYLL GMNITRGCTT KLAQNKFLLS SGRVQMCLLH EIRQRELAIE
210 220 230 240 250
NYREQSYYHL QLITDLGLKP VMKTEDQVLN PSPLKSLGEN LKDQYLTVED
260 270 280 290 300
FKEGTRKQNP KLLYNLTDLY KDAHAQLQIN AETAKKHIQN LYEEGFITYP
310 320 330 340 350
RSSSRHLPTE QVDRVKGVMQ ALAKSRYSLL VQSVDIDAID IKHKTFDDDL
360 370 380 390 400
VSSHFAIIPT TKQYQEEGRP EIEKQLYSLV VKRFVGNFMR PAVYLVRDVS
410 420 430 440 450
LIDAMGNTYQ IKESVLREKG FLEVFQEEVK EESVETFKVP ILQKGQELQI
460 470 480 490 500
YDFELQESKT KKPALHTESS ILTFMETAGR KIDDEHLKEL MKGKRIGTVA
510 520 530 540 550
TEAAFIPVLH EKNFIDIEKG KIITTPIGRA FIEQFPVQQI KDPLYTAEME
560 570 580 590 600
GMIHRIEKNE MSYENFIAQT NAFVQKITQE IIRIPDTVSY NLIETWKKQI
610 620 630 640 650
EVCQCPCGNG IILDRGKFFG CSNHPNCNKG LPKRVKEKTI PTAQVKKLFE
660 670 680 690 700
ENKTDIIKGF KSNGKEFSAY LAFVNGEVSF NLPSVEELSL GQCPKCQKGK
710 720 730 740 750
ILNRKTFFGC SEYQNGCDFM LPAKIKGKKL SDSQIKKLVN NHVTDFINGF
760 770 780 790 800
SGEKGEFTAA IRLKTDLSIC FEFPTTDDRT VGKCPLCQSR VIIGKTNYLC
810 820 830 840 850
EQYKRGCDFI VSGMILEKRI TASQIKKLLE KNMTDTVKGF VSKKTGKSFD
860 870
AKLTYDSTQK RVTFIYEKKK
Length:870
Mass (Da):100,092
Last modified:July 5, 2004 - v2
Checksum:iCA5E6F00219AB9D4
GO

Sequence cautioni

The sequence AAA22859.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAD32445.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAM26155.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97227 Genomic DNA. Translation: AAA22859.1. Different initiation.
AF065404 Genomic DNA. Translation: AAD32445.1. Different initiation.
AE011190 Genomic DNA. Translation: AAM26155.2. Different initiation.
AE017336 Genomic DNA. Translation: AAT35502.1.
PIRiS41543.
RefSeqiNP_052837.1. NC_001496.1.
WP_000520727.1. NZ_JHDR01000038.1.
YP_022474.1. NC_007322.2.

Genome annotation databases

EnsemblBacteriaiAAT35502; AAT35502; GBAA_pXO1_0213.
GeneIDi2820197.
3361898.
KEGGibar:GBAA_pXO1_0213.
PATRICi32583669. VBIBacAnt112616_0199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97227 Genomic DNA. Translation: AAA22859.1 . Different initiation.
AF065404 Genomic DNA. Translation: AAD32445.1 . Different initiation.
AE011190 Genomic DNA. Translation: AAM26155.2 . Different initiation.
AE017336 Genomic DNA. Translation: AAT35502.1 .
PIRi S41543.
RefSeqi NP_052837.1. NC_001496.1.
WP_000520727.1. NZ_JHDR01000038.1.
YP_022474.1. NC_007322.2.

3D structure databases

ProteinModelPortali P40114.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 261594.GBAA_pXO1_0213.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT35502 ; AAT35502 ; GBAA_pXO1_0213 .
GeneIDi 2820197.
3361898.
KEGGi bar:GBAA_pXO1_0213.
PATRICi 32583669. VBIBacAnt112616_0199.

Phylogenomic databases

eggNOGi COG0550.
HOGENOMi HOG000156583.
OMAi NWRSETH.
OrthoDBi EOG60CWM6.

Enzyme and pathway databases

BioCyci ANTHRA:GBAA_PXO1_0213-MONOMER.
BANT261594:GJ7F-5791-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13342. Toprim_Crpt. 2 hits.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bacillus anthracis pXO1 virulence plasmid encodes a type 1 DNA topoisomerase."
    Fouet A., Sirard J.-C., Mock M.
    Mol. Microbiol. 11:471-479(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 7702.
  2. "Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes."
    Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P., Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y., Ricke D., Svensson R., Jackson P.J.
    J. Bacteriol. 181:6509-6515(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sterne.
  3. "Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis."
    Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L., Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P., Fraser C.M.
    Science 296:2028-2033(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Ames / isolate Florida / A2012.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ames ancestor.

Entry informationi

Entry nameiTOP1_BACAN
AccessioniPrimary (citable) accession number: P40114
Secondary accession number(s): Q8KYH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3