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Protein

DNA topoisomerase 1

Gene

topX

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei299O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri603 – 627C4-type 1Add BLAST25
Zinc fingeri693 – 717C4-type 2Add BLAST25
Zinc fingeri784 – 807C4-type 3Add BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
Biological processVirulence
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topX
Synonyms:top1
Ordered Locus Names:pXO1-142, BXA0213, GBAA_pXO1_0213
Encoded oniPlasmid pXO10 Publication
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Plasmid pXO1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451391 – 870DNA topoisomerase 1Add BLAST870

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei35Interaction with DNAPROSITE-ProRule annotation1
Sitei153Interaction with DNAPROSITE-ProRule annotation1
Sitei157Interaction with DNAPROSITE-ProRule annotation1
Sitei301Interaction with DNAPROSITE-ProRule annotation1

Structurei

3D structure databases

ProteinModelPortaliP40114
SMRiP40114
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 128ToprimPROSITE-ProRule annotationAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 185Interaction with DNABy similarity6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri603 – 627C4-type 1Add BLAST25
Zinc fingeri693 – 717C4-type 2Add BLAST25
Zinc fingeri784 – 807C4-type 3Add BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000156583
KOiK03168
OMAiFAYGHLL

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR025589 Toprim_C_rpt
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 2 hits
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF13342 Toprim_Crpt, 3 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

P40114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSPRFRHSQ KYIGSKPYYG YYENDHYIVS WCRGHLLELK NPEEMDPKYK
60 70 80 90 100
LFQLEHLPLI FQPSYKVIQE NAEQLQILVK LLQRPDVDHA VNICDADREG
110 120 130 140 150
ELIYREVYEY AGVNKKQSRV YKSSFEAAEL EAALNRLESA SKYDGLAYSA
160 170 180 190 200
KARQYLDYLL GMNITRGCTT KLAQNKFLLS SGRVQMCLLH EIRQRELAIE
210 220 230 240 250
NYREQSYYHL QLITDLGLKP VMKTEDQVLN PSPLKSLGEN LKDQYLTVED
260 270 280 290 300
FKEGTRKQNP KLLYNLTDLY KDAHAQLQIN AETAKKHIQN LYEEGFITYP
310 320 330 340 350
RSSSRHLPTE QVDRVKGVMQ ALAKSRYSLL VQSVDIDAID IKHKTFDDDL
360 370 380 390 400
VSSHFAIIPT TKQYQEEGRP EIEKQLYSLV VKRFVGNFMR PAVYLVRDVS
410 420 430 440 450
LIDAMGNTYQ IKESVLREKG FLEVFQEEVK EESVETFKVP ILQKGQELQI
460 470 480 490 500
YDFELQESKT KKPALHTESS ILTFMETAGR KIDDEHLKEL MKGKRIGTVA
510 520 530 540 550
TEAAFIPVLH EKNFIDIEKG KIITTPIGRA FIEQFPVQQI KDPLYTAEME
560 570 580 590 600
GMIHRIEKNE MSYENFIAQT NAFVQKITQE IIRIPDTVSY NLIETWKKQI
610 620 630 640 650
EVCQCPCGNG IILDRGKFFG CSNHPNCNKG LPKRVKEKTI PTAQVKKLFE
660 670 680 690 700
ENKTDIIKGF KSNGKEFSAY LAFVNGEVSF NLPSVEELSL GQCPKCQKGK
710 720 730 740 750
ILNRKTFFGC SEYQNGCDFM LPAKIKGKKL SDSQIKKLVN NHVTDFINGF
760 770 780 790 800
SGEKGEFTAA IRLKTDLSIC FEFPTTDDRT VGKCPLCQSR VIIGKTNYLC
810 820 830 840 850
EQYKRGCDFI VSGMILEKRI TASQIKKLLE KNMTDTVKGF VSKKTGKSFD
860 870
AKLTYDSTQK RVTFIYEKKK
Length:870
Mass (Da):100,092
Last modified:July 5, 2004 - v2
Checksum:iCA5E6F00219AB9D4
GO

Sequence cautioni

The sequence AAA22859 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAD32445 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAM26155 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97227 Genomic DNA Translation: AAA22859.1 Different initiation.
AF065404 Genomic DNA Translation: AAD32445.1 Different initiation.
AE011190 Genomic DNA Translation: AAM26155.2 Different initiation.
AE017336 Genomic DNA Translation: AAT35502.1
PIRiS41543
RefSeqiNP_052837.1, NC_001496.1
WP_000520727.1, NZ_NRJB01000015.1

Genome annotation databases

EnsemblBacteriaiAAM26155; AAM26155; BX_A0213
AAT35502; AAT35502; GBAA_pXO1_0213
GeneIDi3361898
KEGGibar:GBAA_pXO1_0213

Similar proteinsi

Entry informationi

Entry nameiTOP1_BACAN
AccessioniPrimary (citable) accession number: P40114
Secondary accession number(s): Q8KYH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome
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Main funding by: National Institutes of Health