Reviewed, UniProtKB/Swiss-Prot P40114 (TOP1_BACAN)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topX
Synonyms:	top1
Ordered Locus Names:	pXO1-142, BXA0213, GBAA_pXO1_0213

Encoded on

Plasmid pXO1

Organism

Bacillus anthracis [Complete proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	870 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. May play an important role in either the maintenance of, or expression from, the virulence plasmid. Its influence on expression could be specific, tuning local superhelicity or, more generally, modifying the superhelicity of the entire plasmid in response to environmental stimuli, thus could be a fundamental factor involved in virulence.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Biological process	Virulence
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 870	870	DNA topoisomerase 1		PRO_0000145139
Regions
	Zinc finger	603 – 627	25	C4-type 1
	Zinc finger	693 – 717	25	C4-type 2
	Zinc finger	784 – 807	24	C4-type 3
Sites
	Active site	299	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40114-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: CA5E6F00219AB9D4	FASTA	870	100,092
	10 20 30 40 50 60 MKSPRFRHSQ KYIGSKPYYG YYENDHYIVS WCRGHLLELK NPEEMDPKYK LFQLEHLPLI 70 80 90 100 110 120 FQPSYKVIQE NAEQLQILVK LLQRPDVDHA VNICDADREG ELIYREVYEY AGVNKKQSRV 130 140 150 160 170 180 YKSSFEAAEL EAALNRLESA SKYDGLAYSA KARQYLDYLL GMNITRGCTT KLAQNKFLLS 190 200 210 220 230 240 SGRVQMCLLH EIRQRELAIE NYREQSYYHL QLITDLGLKP VMKTEDQVLN PSPLKSLGEN 250 260 270 280 290 300 LKDQYLTVED FKEGTRKQNP KLLYNLTDLY KDAHAQLQIN AETAKKHIQN LYEEGFITYP 310 320 330 340 350 360 RSSSRHLPTE QVDRVKGVMQ ALAKSRYSLL VQSVDIDAID IKHKTFDDDL VSSHFAIIPT 370 380 390 400 410 420 TKQYQEEGRP EIEKQLYSLV VKRFVGNFMR PAVYLVRDVS LIDAMGNTYQ IKESVLREKG 430 440 450 460 470 480 FLEVFQEEVK EESVETFKVP ILQKGQELQI YDFELQESKT KKPALHTESS ILTFMETAGR 490 500 510 520 530 540 KIDDEHLKEL MKGKRIGTVA TEAAFIPVLH EKNFIDIEKG KIITTPIGRA FIEQFPVQQI 550 560 570 580 590 600 KDPLYTAEME GMIHRIEKNE MSYENFIAQT NAFVQKITQE IIRIPDTVSY NLIETWKKQI 610 620 630 640 650 660 EVCQCPCGNG IILDRGKFFG CSNHPNCNKG LPKRVKEKTI PTAQVKKLFE ENKTDIIKGF 670 680 690 700 710 720 KSNGKEFSAY LAFVNGEVSF NLPSVEELSL GQCPKCQKGK ILNRKTFFGC SEYQNGCDFM 730 740 750 760 770 780 LPAKIKGKKL SDSQIKKLVN NHVTDFINGF SGEKGEFTAA IRLKTDLSIC FEFPTTDDRT 790 800 810 820 830 840 VGKCPLCQSR VIIGKTNYLC EQYKRGCDFI VSGMILEKRI TASQIKKLLE KNMTDTVKGF 850 860 870 VSKKTGKSFD AKLTYDSTQK RVTFIYEKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacillus anthracis pXO1 virulence plasmid encodes a type 1 DNA topoisomerase." Fouet A., Sirard J.-C., Mock M. Mol. Microbiol. 11:471-479(1994) [PubMed: 8152371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 7702.
[2]	"Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes." Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P., Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y., Ricke D., Svensson R., Jackson P.J. J. Bacteriol. 181:6509-6515(1999) [PubMed: 10515943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.
[3]	"Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis." Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L., Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P., Fraser C.M. Science 296:2028-2033(2002) [PubMed: 12004073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Ames / isolate Florida / A2012.
[4]	"Bacillus anthracis comparative genomics." Ravel J., Rasko D.A., Shumway M.F., Jiang L., Cer R.Z., Federova N.B., Wilson M., Stanley S., Decker S., Read T.D., Salzberg S.L., Fraser C.M. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97227 Genomic DNA. Translation: AAA22859.1. Different initiation. AF065404 Genomic DNA. Translation: AAD32445.1. Different initiation. AE011190 Genomic DNA. Translation: AAM26155.1. AE017336 Genomic DNA. Translation: AAT35502.1.
PIR	S41543.
RefSeq	NP_052837.1. NP_652966.1. YP_022474.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	1158772. 2820197. 3361898.
GenomeReviews	Gene locus GBAA_pXO1_0213 in contig AE017336_GR.
KEGG	bar:GBAA_pXO1_0213.
TIGR	GBAA_pXO1_0213.
Phylogenomic databases
HOGENOM	P40114.
OMA	RTNTEYL.
Enzyme and pathway databases
BioCyc	BANT261594:GBAA_PXO1_0200-MON.
BRENDA	5.99.1.2. 267517.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_BACAN

Accession

Primary (citable) accession number: P40114
Secondary accession number(s): Q8KYH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)