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P40114 (TOP1_BACAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topX
Synonyms:top1
Ordered Locus Names:pXO1-142, BXA0213, GBAA_pXO1_0213
Encoded onPlasmid pXO1
OrganismBacillus anthracis [Reference proteome] [HAMAP]
Taxonomic identifier1392 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence caution

The sequence AAA22859.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAD32445.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAM26155.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processVirulence
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Plasmid
Reference proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870DNA topoisomerase 1
PRO_0000145139

Regions

Domain1 – 128128Toprim
Zinc finger603 – 62725C4-type 1
Zinc finger693 – 71725C4-type 2
Zinc finger784 – 80724C4-type 3
Region180 – 1856Interaction with DNA By similarity

Sites

Active site2991O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Site351Interaction with DNA By similarity
Site1531Interaction with DNA By similarity
Site1571Interaction with DNA By similarity
Site3011Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P40114 [UniParc].

Last modified July 5, 2004. Version 2.
Checksum: CA5E6F00219AB9D4

FASTA870100,092
        10         20         30         40         50         60 
MKSPRFRHSQ KYIGSKPYYG YYENDHYIVS WCRGHLLELK NPEEMDPKYK LFQLEHLPLI 

        70         80         90        100        110        120 
FQPSYKVIQE NAEQLQILVK LLQRPDVDHA VNICDADREG ELIYREVYEY AGVNKKQSRV 

       130        140        150        160        170        180 
YKSSFEAAEL EAALNRLESA SKYDGLAYSA KARQYLDYLL GMNITRGCTT KLAQNKFLLS 

       190        200        210        220        230        240 
SGRVQMCLLH EIRQRELAIE NYREQSYYHL QLITDLGLKP VMKTEDQVLN PSPLKSLGEN 

       250        260        270        280        290        300 
LKDQYLTVED FKEGTRKQNP KLLYNLTDLY KDAHAQLQIN AETAKKHIQN LYEEGFITYP 

       310        320        330        340        350        360 
RSSSRHLPTE QVDRVKGVMQ ALAKSRYSLL VQSVDIDAID IKHKTFDDDL VSSHFAIIPT 

       370        380        390        400        410        420 
TKQYQEEGRP EIEKQLYSLV VKRFVGNFMR PAVYLVRDVS LIDAMGNTYQ IKESVLREKG 

       430        440        450        460        470        480 
FLEVFQEEVK EESVETFKVP ILQKGQELQI YDFELQESKT KKPALHTESS ILTFMETAGR 

       490        500        510        520        530        540 
KIDDEHLKEL MKGKRIGTVA TEAAFIPVLH EKNFIDIEKG KIITTPIGRA FIEQFPVQQI 

       550        560        570        580        590        600 
KDPLYTAEME GMIHRIEKNE MSYENFIAQT NAFVQKITQE IIRIPDTVSY NLIETWKKQI 

       610        620        630        640        650        660 
EVCQCPCGNG IILDRGKFFG CSNHPNCNKG LPKRVKEKTI PTAQVKKLFE ENKTDIIKGF 

       670        680        690        700        710        720 
KSNGKEFSAY LAFVNGEVSF NLPSVEELSL GQCPKCQKGK ILNRKTFFGC SEYQNGCDFM 

       730        740        750        760        770        780 
LPAKIKGKKL SDSQIKKLVN NHVTDFINGF SGEKGEFTAA IRLKTDLSIC FEFPTTDDRT 

       790        800        810        820        830        840 
VGKCPLCQSR VIIGKTNYLC EQYKRGCDFI VSGMILEKRI TASQIKKLLE KNMTDTVKGF 

       850        860        870 
VSKKTGKSFD AKLTYDSTQK RVTFIYEKKK 

« Hide

References

« Hide 'large scale' references
[1]"Bacillus anthracis pXO1 virulence plasmid encodes a type 1 DNA topoisomerase."
Fouet A., Sirard J.-C., Mock M.
Mol. Microbiol. 11:471-479(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 7702.
[2]"Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes."
Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P., Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y., Ricke D., Svensson R., Jackson P.J.
J. Bacteriol. 181:6509-6515(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sterne.
[3]"Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis."
Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L., Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P., Fraser C.M.
Science 296:2028-2033(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Ames / isolate Florida / A2012.
[4]"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'."
Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.
J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ames ancestor.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M97227 Genomic DNA. Translation: AAA22859.1. Different initiation.
AF065404 Genomic DNA. Translation: AAD32445.1. Different initiation.
AE011190 Genomic DNA. Translation: AAM26155.2. Different initiation.
AE017336 Genomic DNA. Translation: AAT35502.1.
PIRS41543.
RefSeqNP_052837.1. NC_001496.1.
YP_022474.1. NC_007322.2.

3D structure databases

ProteinModelPortalP40114.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING261594.GBAA_pXO1_0213.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT35502; AAT35502; GBAA_pXO1_0213.
GeneID2820197.
3361898.
KEGGbar:GBAA_pXO1_0213.
PATRIC32583669. VBIBacAnt112616_0199.

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHOG000156583.
OMANWRSETH.
OrthoDBEOG60CWM6.

Enzyme and pathway databases

BioCycANTHRA:GBAA_PXO1_0213-MONOMER.
BANT261594:GJ7F-5791-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13342. Toprim_Crpt. 2 hits.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_BACAN
AccessionPrimary (citable) accession number: P40114
Secondary accession number(s): Q8KYH4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families