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P40112

- PSB3_RAT

UniProt

P40112 - PSB3_RAT

Protein

Proteasome subunit beta type-3

Gene

Psmb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Protein family/group databases

    MEROPSiT01.983.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-3 (EC:3.4.25.1)
    Alternative name(s):
    Proteasome chain 13
    Proteasome component C10-II
    Proteasome theta chain
    Gene namesi
    Name:Psmb3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61875. Psmb3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 205204Proteasome subunit beta type-3PRO_0000148059Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei77 – 771N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP40112.
    PRIDEiP40112.

    2D gel databases

    World-2DPAGE0004:P40112.

    PTM databases

    PhosphoSiteiP40112.

    Expressioni

    Gene expression databases

    GenevestigatoriP40112.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

    Protein-protein interaction databases

    IntActiP40112. 1 interaction.
    MINTiMINT-4569088.
    STRINGi10116.ENSRNOP00000017377.

    Structurei

    3D structure databases

    ProteinModelPortaliP40112.
    SMRiP40112. Positions 2-205.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000090523.
    HOVERGENiHBG004446.
    InParanoidiP40112.
    KOiK02735.
    PhylomeDBiP40112.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40112-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY    50
    IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF 100
    GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCSEQMYGMC 150
    ESLWEPNMDP EHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL 200
    KARMD 205
    Length:205
    Mass (Da):22,965
    Last modified:February 1, 1995 - v1
    Checksum:i2197685F3089F7FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21800 mRNA. Translation: BAA04824.1.
    BC084723 mRNA. Translation: AAH84723.1.
    PIRiS40468.
    RefSeqiNP_058981.1. NM_017285.1.
    UniGeneiRn.94551.

    Genome annotation databases

    GeneIDi29676.
    KEGGirno:29676.
    UCSCiRGD:61875. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D21800 mRNA. Translation: BAA04824.1 .
    BC084723 mRNA. Translation: AAH84723.1 .
    PIRi S40468.
    RefSeqi NP_058981.1. NM_017285.1.
    UniGenei Rn.94551.

    3D structure databases

    ProteinModelPortali P40112.
    SMRi P40112. Positions 2-205.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P40112. 1 interaction.
    MINTi MINT-4569088.
    STRINGi 10116.ENSRNOP00000017377.

    Protein family/group databases

    MEROPSi T01.983.

    PTM databases

    PhosphoSitei P40112.

    2D gel databases

    World-2DPAGE 0004:P40112.

    Proteomic databases

    PaxDbi P40112.
    PRIDEi P40112.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29676.
    KEGGi rno:29676.
    UCSCi RGD:61875. rat.

    Organism-specific databases

    CTDi 5691.
    RGDi 61875. Psmb3.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000090523.
    HOVERGENi HBG004446.
    InParanoidi P40112.
    KOi K02735.
    PhylomeDBi P40112.

    Miscellaneous databases

    NextBioi 610015.
    PROi P40112.

    Gene expression databases

    Genevestigatori P40112.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of rat proteasome subunit RC10-II, assumed to be responsible for trypsin-like catalytic activity."
      Nishimura C., Tamura T., Akioka H., Tokunaga F., Tanaka K., Ichihara A.
      FEBS Lett. 336:462-466(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 119-143.
      Tissue: Embryonic brain and Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 28-41; 49-66 AND 100-115, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    4. Lubec G., Chen W.-Q.
      Submitted (FEB-2007) to UniProtKB
      Cited for: ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPSB3_RAT
    AccessioniPrimary (citable) accession number: P40112
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3