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Protein

Flavin-dependent thymidylate synthase

Gene

thyX

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.UniRule annotation

Cofactori

FADUniRule annotationNote: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103FAD; shared with neighboring subunitsUniRule annotation1
Binding sitei172dUMPUniRule annotation1
Binding sitei194FADUniRule annotation1
Active sitei199Involved in ionization of N3 of dUMP, leading to its activationUniRule annotation1
Binding sitei199dUMP; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 95dUMP; shared with dimeric partnerUniRule annotation4
Nucleotide bindingi95 – 97FADUniRule annotation3
Nucleotide bindingi103 – 107dUMPUniRule annotation5
Nucleotide bindingi188 – 190FAD; shared with neighboring subunitsUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciCORYNE:G18NG-11564-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavin-dependent thymidylate synthaseUniRule annotation (EC:2.1.1.148UniRule annotation)
Short name:
FDTSUniRule annotation
Alternative name(s):
FAD-dependent thymidylate synthaseUniRule annotation
Thymidylate synthase ThyXUniRule annotation
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyXUniRule annotation
Ordered Locus Names:Cgl1972, cg2162
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001755601 – 250Flavin-dependent thymidylate synthaseAdd BLAST250

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg2162.

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi32 – 44Combined sources13
Helixi57 – 66Combined sources10
Helixi71 – 75Combined sources5
Beta strandi77 – 86Combined sources10
Helixi87 – 93Combined sources7
Beta strandi99 – 103Combined sources5
Helixi120 – 123Combined sources4
Helixi126 – 152Combined sources27
Helixi165 – 171Combined sources7
Helixi172 – 174Combined sources3
Beta strandi179 – 188Combined sources10
Helixi189 – 199Combined sources11
Helixi206 – 222Combined sources17
Helixi224 – 227Combined sources4
Beta strandi231 – 234Combined sources4
Beta strandi240 – 243Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FNNX-ray2.30A1-250[»]
3GE9X-ray2.61A1-250[»]
ProteinModelPortaliP40111.
SMRiP40111.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40111.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 233ThyXUniRule annotationAdd BLAST227

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 105ThyX motifUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.UniRule annotation
Contains 1 thyX (flavin-dependent thymidylate synthase) domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107SMJ. Bacteria.
COG1351. LUCA.
KOiK03465.
OMAiACYQSWN.

Family and domain databases

HAMAPiMF_01408. ThyX. 1 hit.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQVKLSVE LIACSSFTPP ADVEWSTDVE GAEALVEFAG RACYETFDKP
60 70 80 90 100
NPRTASNAAY LRHIMEVGHT ALLEHANATM YIRGISRSAT HELVRHRHFS
110 120 130 140 150
FSQLSQRFVH SGESEVVVPT LIDEDPQLRE LFMHAMDESR FAFNELLNAL
160 170 180 190 200
EEKLGDEPNA LLRKKQARQA ARAVLPNATE SRIVVSGNFR TWRHFIGMRA
210 220 230 240 250
SEHADVEIRE VAVECLRKLQ VAAPTVFGDF EIETLADGSQ MATSPYVMDF
Length:250
Mass (Da):28,065
Last modified:July 11, 2002 - v2
Checksum:iE6C8FF5276BE6314
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214E → G in CAA79713 (PubMed:8478336).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21502 Genomic DNA. Translation: CAA79713.1.
BA000036 Genomic DNA. Translation: BAB99365.1.
BX927153 Genomic DNA. Translation: CAF20313.1.
PIRiB40626.
RefSeqiNP_601178.1. NC_003450.3.
WP_011014793.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99365; BAB99365; BAB99365.
CAF20313; CAF20313; cg2162.
GeneIDi1019929.
KEGGicgb:cg2162.
cgl:NCgl1897.
PATRICi21495946. VBICorGlu203724_1909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21502 Genomic DNA. Translation: CAA79713.1.
BA000036 Genomic DNA. Translation: BAB99365.1.
BX927153 Genomic DNA. Translation: CAF20313.1.
PIRiB40626.
RefSeqiNP_601178.1. NC_003450.3.
WP_011014793.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FNNX-ray2.30A1-250[»]
3GE9X-ray2.61A1-250[»]
ProteinModelPortaliP40111.
SMRiP40111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2162.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99365; BAB99365; BAB99365.
CAF20313; CAF20313; cg2162.
GeneIDi1019929.
KEGGicgb:cg2162.
cgl:NCgl1897.
PATRICi21495946. VBICorGlu203724_1909.

Phylogenomic databases

eggNOGiENOG4107SMJ. Bacteria.
COG1351. LUCA.
KOiK03465.
OMAiACYQSWN.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciCORYNE:G18NG-11564-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40111.

Family and domain databases

HAMAPiMF_01408. ThyX. 1 hit.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHYX_CORGL
AccessioniPrimary (citable) accession number: P40111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2002
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.