Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase ThyX

Gene

thyX

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase ThyX (EC:2.1.1.148)
Short name:
TS
Short name:
TSase
Gene namesi
Name:thyX
Ordered Locus Names:Cgl1972, cg2162
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250Thymidylate synthase ThyXPRO_0000175560Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi196627.cg2162.

Structurei

Secondary structure

1
250
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147Combined sources
Helixi32 – 4413Combined sources
Helixi57 – 6610Combined sources
Helixi71 – 755Combined sources
Beta strandi77 – 8610Combined sources
Helixi87 – 937Combined sources
Beta strandi99 – 1035Combined sources
Helixi120 – 1234Combined sources
Helixi126 – 15227Combined sources
Helixi165 – 1717Combined sources
Helixi172 – 1743Combined sources
Beta strandi179 – 18810Combined sources
Helixi189 – 19911Combined sources
Helixi206 – 22217Combined sources
Helixi224 – 2274Combined sources
Beta strandi231 – 2344Combined sources
Beta strandi240 – 2434Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FNNX-ray2.30A1-250[»]
3GE9X-ray2.61A1-250[»]
ProteinModelPortaliP40111.
SMRiP40111. Positions 3-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40111.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 233227ThyXAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi95 – 10511ThyX motifAdd
BLAST

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.Curated

Phylogenomic databases

eggNOGiENOG4107SMJ. Bacteria.
COG1351. LUCA.
KOiK03465.
OMAiACYQSWN.
OrthoDBiEOG6SFP8J.

Family and domain databases

HAMAPiMF_01408. ThyX.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQVKLSVE LIACSSFTPP ADVEWSTDVE GAEALVEFAG RACYETFDKP
60 70 80 90 100
NPRTASNAAY LRHIMEVGHT ALLEHANATM YIRGISRSAT HELVRHRHFS
110 120 130 140 150
FSQLSQRFVH SGESEVVVPT LIDEDPQLRE LFMHAMDESR FAFNELLNAL
160 170 180 190 200
EEKLGDEPNA LLRKKQARQA ARAVLPNATE SRIVVSGNFR TWRHFIGMRA
210 220 230 240 250
SEHADVEIRE VAVECLRKLQ VAAPTVFGDF EIETLADGSQ MATSPYVMDF
Length:250
Mass (Da):28,065
Last modified:July 11, 2002 - v2
Checksum:iE6C8FF5276BE6314
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti214 – 2141E → G in CAA79713 (PubMed:8478336).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21502 Genomic DNA. Translation: CAA79713.1.
BA000036 Genomic DNA. Translation: BAB99365.1.
BX927153 Genomic DNA. Translation: CAF20313.1.
PIRiB40626.
RefSeqiNP_601178.1. NC_003450.3.
WP_011014793.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99365; BAB99365; BAB99365.
CAF20313; CAF20313; cg2162.
GeneIDi1019929.
KEGGicgb:cg2162.
cgl:NCgl1897.
PATRICi21495946. VBICorGlu203724_1909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21502 Genomic DNA. Translation: CAA79713.1.
BA000036 Genomic DNA. Translation: BAB99365.1.
BX927153 Genomic DNA. Translation: CAF20313.1.
PIRiB40626.
RefSeqiNP_601178.1. NC_003450.3.
WP_011014793.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FNNX-ray2.30A1-250[»]
3GE9X-ray2.61A1-250[»]
ProteinModelPortaliP40111.
SMRiP40111. Positions 3-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg2162.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB99365; BAB99365; BAB99365.
CAF20313; CAF20313; cg2162.
GeneIDi1019929.
KEGGicgb:cg2162.
cgl:NCgl1897.
PATRICi21495946. VBICorGlu203724_1909.

Phylogenomic databases

eggNOGiENOG4107SMJ. Bacteria.
COG1351. LUCA.
KOiK03465.
OMAiACYQSWN.
OrthoDBiEOG6SFP8J.

Miscellaneous databases

EvolutionaryTraceiP40111.

Family and domain databases

HAMAPiMF_01408. ThyX.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function."
    Pisabarro A., Malumbres M., Mateos L.M., Oguiza J.A., Martin J.F.
    J. Bacteriol. 175:2743-2749(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiTHYX_CORGL
AccessioniPrimary (citable) accession number: P40111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 11, 2002
Last modified: November 11, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.