P40110 (DAPB_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate reductase
Short name=DHPR
EC=1.3.1.26

Gene names

Name:	dapB
Ordered Locus Names:	Cgl1973, cg2163

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ = (S)-2,3-dihydrodipicolinate + NAD(P)H. HAMAP MF_00102
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. HAMAP MF_00102
Subcellular location	Cytoplasm By similarity HAMAP MF_00102.
Sequence similarities	Belongs to the dihydrodipicolinate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADPH binding Inferred from electronic annotation. Source: InterPro dihydrodipicolinate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Dihydrodipicolinate reductase HAMAP MF_00102		PRO_0000141435
Experimental info
	Sequence conflict	91	1	D → A in CAA79712. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P40110 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: A0B143F478D12414	FASTA	248	26,024
	10 20 30 40 50 60 MGIKVGVLGA KGRVGQTIVA AVNESDDLEL VAEIGVDDDL SLLVDNGAEV VVDFTTPNAV 70 80 90 100 110 120 MGNLEFCINN GISAVVGTTG FDDARLEQVR DWLEGKDNVG VLIAPNFAIS AVLTMVFSKQ 130 140 150 160 170 180 AARFFESAEV IELHHPNKLD APSGTAIHTA QGIAAARKEA GMDAQPDATE QALEGSRGAS 190 200 210 220 230 240 VDGIPVHAVR MSGMVAHEQV IFGTQGQTLT IKQDSYDRNS FAPGVLVGVR NIAQHPGLVV GLEHYLGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Eikmanns B.J., Eggeling L., Thum-Schmitz N., Krumbach K. Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function." Pisabarro A., Malumbres M., Mateos L.M., Oguiza J.A., Martin J.F. J. Bacteriol. 175:2743-2749(1993) [PubMed: 8478336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67737 Genomic DNA. Translation: CAA47968.1. Z21502 Genomic DNA. Translation: CAA79712.1. BA000036 Genomic DNA. Translation: BAB99366.1. BX927153 Genomic DNA. Translation: CAF20314.1.
PIR	A40626.
RefSeq	NP_601179.1. NC_003450.3. YP_226215.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P40110.
SMR	P40110. Positions 3-248.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1019930. 3345049.
GenomeReviews	Gene locus Cgl1973 in contig BA000036_GR. Gene locus cg2163 in contig BX927147_GR.
KEGG	cgb:cg2163. cgl:NCgl1898.
PATRIC	21495948. VBICorGlu203724_1910.
Phylogenomic databases
HOGENOM	HBG594002.
OMA	DRASFMP.
PhylomeDB	P40110.
ProtClustDB	PRK00048.
Enzyme and pathway databases
BioCyc	CGLU196627:CG2163-MONOMER. MetaCyc:MONOMER-6445.
Family and domain databases
HAMAP	MF_00102. DapB. [Tree]
InterPro	IPR022663. DapB_C. IPR000846. DapB_N. IPR022664. DapB_N_CS. IPR011770. Dihydrodipicolinate_Rdtase. IPR023940. Dihydrodipicolinate_Rdtase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00215.
PANTHER	PTHR20836. DapB_bac/pln. 1 hit.
Pfam	PF05173. DapB_C. 1 hit. PF01113. DapB_N. 1 hit. [Graphical view]
PIRSF	PIRSF000161. DHPR. 1 hit.
TIGRFAMs	TIGR00036. DapB. 1 hit.
PROSITE	PS01298. DAPB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_CORGL

Accession

Primary (citable) accession number: P40110
Secondary accession number(s): P42462

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 11, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program