Reviewed, UniProtKB/Swiss-Prot P40106 (GPP2_YEAST)

Names and origin

Protein names

Recommended name:
    (DL)-glycerol-3-phosphatase 2
    EC=3.1.3.-

Gene names

Name:	GPP2
Synonyms:	HOR2
Ordered Locus Names:	YER062C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Glycerol 3-phosphate + H2O = glycerol + phosphate.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Induction	By osmotic stress.
Miscellaneous	Present with 5000 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Ref.3 Inferred from direct assay. Source: SGD glycerol biosynthetic process Inferred from mutant phenotype. Source: SGD response to osmotic stress Ref.3 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	glycerol-1-phosphatase activity Ref.3 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
URN1	Q06525	1	EBI-7836,EBI-35138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	(DL)-glycerol-3-phosphatase 2		PRO_0000087561
Amino acid modifications
	Modified residue	115	1	Phosphothreonine Ref.5
	Modified residue	116	1	Phosphoserine Ref.5
	Modified residue	118	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P40106-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D69F343B10417313	FASTA	250	27,814
	10 20 30 40 50 60 MGLTTKPLSL KVNAALFDVD GTIIISQPAI AAFWRDFGKD KPYFDAEHVI QVSHGWRTFD 70 80 90 100 110 120 AIAKFAPDFA NEEYVNKLEA EIPVKYGEKS IEVPGAVKLC NALNALPKEK WAVATSGTRD 130 140 150 160 170 180 MAQKWFEHLG IRRPKYFITA NDVKQGKPHP EPYLKGRNGL GYPINEQDPS KSKVVVFEDA 190 200 210 220 230 240 PAGIAAGKAA GCKIIGIATT FDLDFLKEKG CDIIVKNHES IRVGGYNAET DEVEFIFDDY 250 LYAKDDLLKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae." Hirayama T., Maeda T., Saito H., Shinozaki K. Mol. Gen. Genet. 249:127-138(1995) [PubMed: 7500933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: RS16.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway." Norbeck J., Paehlman A.-K., Akhtar N., Blomberg A., Adler L. J. Biol. Chem. 271:13875-13881(1996) [PubMed: 8662716] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-115; SER-116 AND THR-118, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50469 mRNA. Translation: BAA09058.1. U18813 Genomic DNA. Translation: AAB64598.1.
PIR	S50565.
RefSeq	NP_010984.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1366N.
IntAct	P40106. 7 interactions.
Proteomic databases
PeptideAtlas	P40106.
PRIDE	P40106.
Genome annotation databases
Ensembl	YER062C. Saccharomyces cerevisiae. [Contig view]
GeneID	856791.
GenomeReviews	Gene locus YER062C in contig U00092_GR.
KEGG	sce:YER062C.
NMPDR	fig|4932.3.peg.2048.
Organism-specific databases
CYGD	YER062c.
SGD	S000000864. HOR2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P40106.
OMA	P40106. NTHAVNG.
Gene expression databases
ArrayExpress	P40106.
GermOnline	YER062C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR006402. HAD-SF_hydro_IA_v3. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01509. HAD-SF-IA-v3. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	983021.

Entry information

Entry name

GPP2_YEAST

Accession

Primary (citable) accession number: P40106

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)