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Protein

Glycerol-1-phosphate phosphohydrolase 2

Gene

GPP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation.1 Publication

Miscellaneous

Present with 5000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Glycerol 1-phosphate + H2O = glycerol + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=3.9 mM for (DL)-glycerol 3-phosphate1 Publication
  1. Vmax=46 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18NucleophileBy similarity1
Metal bindingi18MagnesiumBy similarity1
Active sitei20Proton donorBy similarity1
Metal bindingi20MagnesiumBy similarity1
Metal bindingi179MagnesiumBy similarity1

GO - Molecular functioni

  • glycerol-1-phosphatase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular carbohydrate metabolic process Source: SGD
  • glycerol biosynthetic process Source: SGD
  • response to osmotic stress Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processStress response
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YER062C-MONOMER
YEAST:YER062C-MONOMER
BRENDAi3.1.3.21 984

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-1-phosphate phosphohydrolase 2Curated (EC:3.1.3.211 Publication)
Alternative name(s):
(DL)-glycerol-3-phosphatase 21 Publication
Hyperosmolarity-responsive protein 21 Publication
Gene namesi
Name:GPP21 Publication
Synonyms:HOR21 Publication
Ordered Locus Names:YER062C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER062C
SGDiS000000864 GPP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000875611 – 250Glycerol-1-phosphate phosphohydrolase 2Add BLAST250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei90PhosphoserineBy similarity1
Cross-linki144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40106
PaxDbiP40106
PRIDEiP40106

PTM databases

iPTMnetiP40106

Expressioni

Inductioni

By osmotic stress (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi36804, 88 interactors
DIPiDIP-1366N
IntActiP40106, 8 interactors
MINTiP40106
STRINGi4932.YER062C

Structurei

3D structure databases

ProteinModelPortaliP40106
SMRiP40106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000065392
HOGENOMiHOG000248341
InParanoidiP40106
KOiK06117
OMAiDPQCYIL
OrthoDBiEOG092C47K9

Family and domain databases

Gene3Di1.10.150.240, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR006439 HAD-SF_hydro_IA
IPR023214 HAD_sf
IPR023198 PGP-like_dom2
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01509 HAD-SF-IA-v3, 1 hit

Sequencei

Sequence statusi: Complete.

P40106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTTKPLSL KVNAALFDVD GTIIISQPAI AAFWRDFGKD KPYFDAEHVI
60 70 80 90 100
QVSHGWRTFD AIAKFAPDFA NEEYVNKLEA EIPVKYGEKS IEVPGAVKLC
110 120 130 140 150
NALNALPKEK WAVATSGTRD MAQKWFEHLG IRRPKYFITA NDVKQGKPHP
160 170 180 190 200
EPYLKGRNGL GYPINEQDPS KSKVVVFEDA PAGIAAGKAA GCKIIGIATT
210 220 230 240 250
FDLDFLKEKG CDIIVKNHES IRVGGYNAET DEVEFIFDDY LYAKDDLLKW
Length:250
Mass (Da):27,814
Last modified:February 1, 1995 - v1
Checksum:iD69F343B10417313
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50469 mRNA Translation: BAA09058.1
U18813 Genomic DNA Translation: AAB64598.1
BK006939 Genomic DNA Translation: DAA07721.1
PIRiS50565
RefSeqiNP_010984.3, NM_001178953.3

Genome annotation databases

EnsemblFungiiYER062C; YER062C; YER062C
GeneIDi856791
KEGGisce:YER062C

Similar proteinsi

Entry informationi

Entry nameiGPP2_YEAST
AccessioniPrimary (citable) accession number: P40106
Secondary accession number(s): D3DLW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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