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Protein

Glycerol-1-phosphate phosphohydrolase 2

Gene

GPP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation.1 Publication

Catalytic activityi

Glycerol 1-phosphate + H2O = glycerol + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=3.9 mM for (DL)-glycerol 3-phosphate1 Publication
  1. Vmax=46 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 6.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei18 – 181NucleophileBy similarity
Metal bindingi18 – 181MagnesiumBy similarity
Active sitei20 – 201Proton donorBy similarity
Metal bindingi20 – 201MagnesiumBy similarity
Metal bindingi179 – 1791MagnesiumBy similarity

GO - Molecular functioni

  • glycerol-1-phosphatase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular carbohydrate metabolic process Source: SGD
  • glycerol biosynthetic process Source: SGD
  • response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YER062C-MONOMER.
YEAST:YER062C-MONOMER.
BRENDAi3.1.3.21. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-1-phosphate phosphohydrolase 2Curated (EC:3.1.3.211 Publication)
Alternative name(s):
(DL)-glycerol-3-phosphatase 21 Publication
Hyperosmolarity-responsive protein 21 Publication
Gene namesi
Name:GPP21 Publication
Synonyms:HOR21 Publication
Ordered Locus Names:YER062C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER062C.
SGDiS000000864. GPP2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 250249Glycerol-1-phosphate phosphohydrolase 2PRO_0000087561Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki64 – 64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei90 – 901PhosphoserineBy similarity
Cross-linki144 – 144Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40106.

PTM databases

iPTMnetiP40106.

Expressioni

Inductioni

By osmotic stress (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi36804. 67 interactions.
DIPiDIP-1366N.
IntActiP40106. 4 interactions.
MINTiMINT-401307.

Structurei

3D structure databases

ProteinModelPortaliP40106.
SMRiP40106. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248341.
InParanoidiP40106.
KOiK06117.
OMAiNTHAVNG.
OrthoDBiEOG092C47K9.

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTTKPLSL KVNAALFDVD GTIIISQPAI AAFWRDFGKD KPYFDAEHVI
60 70 80 90 100
QVSHGWRTFD AIAKFAPDFA NEEYVNKLEA EIPVKYGEKS IEVPGAVKLC
110 120 130 140 150
NALNALPKEK WAVATSGTRD MAQKWFEHLG IRRPKYFITA NDVKQGKPHP
160 170 180 190 200
EPYLKGRNGL GYPINEQDPS KSKVVVFEDA PAGIAAGKAA GCKIIGIATT
210 220 230 240 250
FDLDFLKEKG CDIIVKNHES IRVGGYNAET DEVEFIFDDY LYAKDDLLKW
Length:250
Mass (Da):27,814
Last modified:February 1, 1995 - v1
Checksum:iD69F343B10417313
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50469 mRNA. Translation: BAA09058.1.
U18813 Genomic DNA. Translation: AAB64598.1.
BK006939 Genomic DNA. Translation: DAA07721.1.
PIRiS50565.
RefSeqiNP_010984.3. NM_001178953.3.

Genome annotation databases

EnsemblFungiiYER062C; YER062C; YER062C.
GeneIDi856791.
KEGGisce:YER062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50469 mRNA. Translation: BAA09058.1.
U18813 Genomic DNA. Translation: AAB64598.1.
BK006939 Genomic DNA. Translation: DAA07721.1.
PIRiS50565.
RefSeqiNP_010984.3. NM_001178953.3.

3D structure databases

ProteinModelPortaliP40106.
SMRiP40106. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36804. 67 interactions.
DIPiDIP-1366N.
IntActiP40106. 4 interactions.
MINTiMINT-401307.

PTM databases

iPTMnetiP40106.

Proteomic databases

MaxQBiP40106.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER062C; YER062C; YER062C.
GeneIDi856791.
KEGGisce:YER062C.

Organism-specific databases

EuPathDBiFungiDB:YER062C.
SGDiS000000864. GPP2.

Phylogenomic databases

HOGENOMiHOG000248341.
InParanoidiP40106.
KOiK06117.
OMAiNTHAVNG.
OrthoDBiEOG092C47K9.

Enzyme and pathway databases

BioCyciMetaCyc:YER062C-MONOMER.
YEAST:YER062C-MONOMER.
BRENDAi3.1.3.21. 984.

Miscellaneous databases

PROiP40106.

Family and domain databases

Gene3Di1.10.150.240. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
IPR023198. PGP_dom2.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGPP2_YEAST
AccessioniPrimary (citable) accession number: P40106
Secondary accession number(s): D3DLW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.