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P40099

- FTHC_YEAST

UniProt

P40099 - FTHC_YEAST

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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene
FAU1, YER183C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Only enzyme known to utilize 5-formyltetrahydrofolate (folinic acid) as substrate. Contributes to tetrahydrofolate metabolism in an alternative way of folate biosynthesis. May regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids.

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Kineticsi

  1. KM=43 µM for ATP1 Publication
  2. KM=33 µM for 5-formyltetrahydrofolate

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561Substrate By similarity
Binding sitei194 – 1941ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 85ATP By similarity
Nucleotide bindingi151 – 1588ATP By similarity

GO - Molecular functioni

  1. 5-formyltetrahydrofolate cyclo-ligase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER183C-MONOMER.
SABIO-RKP40099.

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Alternative name(s):
5,10-methenyl-tetrahydrofolate synthetase
Short name:
MTHFS
Short name:
Methenyl-THF synthetase
Gene namesi
Name:FAU1
Ordered Locus Names:YER183C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER183c.
SGDiS000000985. FAU1.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Abolishes methenyltetrahydrofolate synthase activity and leads to accumulation of folinic acid. Leads to a striking methionine deficiency when combined with ADE16 and ADE17, 2 isoenzymes involved in the purine synthesis.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2112115-formyltetrahydrofolate cyclo-ligasePRO_0000200280Add
BLAST

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP40099.
PaxDbiP40099.

Expressioni

Gene expression databases

GenevestigatoriP40099.

Interactioni

Protein-protein interaction databases

BioGridi36937. 17 interactions.
IntActiP40099. 1 interaction.
MINTiMINT-4484715.
STRINGi4932.YER183C.

Structurei

3D structure databases

ProteinModelPortaliP40099.
SMRiP40099. Positions 26-190.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1565Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0212.
GeneTreeiENSGT00390000017791.
HOGENOMiHOG000007299.
KOiK01934.
OMAiSCYLSMP.
OrthoDBiEOG7FBRWF.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P40099-1 [UniParc]FASTAAdd to Basket

« Hide

MATKQLLRRQ IKRVINALDY DIIAAESHTI SQAVRSLIAS ANSRRVACYM    50
SMDKGEVTTG EIIKNLFQDG QEVFLPRCTH TSESKHFKLR EDHHPHLIFH 100
RMSSLKMVRD LKPQGPYQLK EPEPHIEESD ILDVVLVPGV AFDIKTGARM 150
GHGAGYYDDF FQRYKILHEG QKPLLVGLCL MEQVASPIPL EKHDYSMDCI 200
VCGDGSIHWF Q 211
Length:211
Mass (Da):24,059
Last modified:February 1, 1995 - v1
Checksum:i96F0D84B3B69DD57
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18922 Genomic DNA. Translation: AAB64710.1.
BK006939 Genomic DNA. Translation: DAA07846.1.
PIRiS50686.
RefSeqiNP_011110.1. NM_001179073.1.

Genome annotation databases

EnsemblFungiiYER183C; YER183C; YER183C.
GeneIDi856932.
KEGGisce:YER183C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18922 Genomic DNA. Translation: AAB64710.1 .
BK006939 Genomic DNA. Translation: DAA07846.1 .
PIRi S50686.
RefSeqi NP_011110.1. NM_001179073.1.

3D structure databases

ProteinModelPortali P40099.
SMRi P40099. Positions 26-190.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36937. 17 interactions.
IntActi P40099. 1 interaction.
MINTi MINT-4484715.
STRINGi 4932.YER183C.

Proteomic databases

MaxQBi P40099.
PaxDbi P40099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER183C ; YER183C ; YER183C .
GeneIDi 856932.
KEGGi sce:YER183C.

Organism-specific databases

CYGDi YER183c.
SGDi S000000985. FAU1.

Phylogenomic databases

eggNOGi COG0212.
GeneTreei ENSGT00390000017791.
HOGENOMi HOG000007299.
KOi K01934.
OMAi SCYLSMP.
OrthoDBi EOG7FBRWF.

Enzyme and pathway databases

BioCyci YEAST:YER183C-MONOMER.
SABIO-RK P40099.

Miscellaneous databases

NextBioi 983412.
PROi P40099.

Gene expression databases

Genevestigatori P40099.

Family and domain databases

Gene3Di 3.40.50.10420. 1 hit.
InterProi IPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view ]
PANTHERi PTHR23407:SF1. PTHR23407:SF1. 1 hit.
Pfami PF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view ]
PIRSFi PIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsi TIGR02727. MTHFS_bact. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Cloning and characterization of methenyltetrahydrofolate synthetase from Saccharomyces cerevisiae."
    Holmes W.B., Appling D.R.
    J. Biol. Chem. 277:20205-20213(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  6. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
    Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
    Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFTHC_YEAST
AccessioniPrimary (citable) accession number: P40099
Secondary accession number(s): D3DM92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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