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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

FAU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Only enzyme known to utilize 5-formyltetrahydrofolate (folinic acid) as substrate. Contributes to tetrahydrofolate metabolism in an alternative way of folate biosynthesis. May regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids.1 Publication

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

Kineticsi

  1. KM=43 µM for ATP1 Publication
  2. KM=33 µM for 5-formyltetrahydrofolate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei56SubstrateBy similarity1
    Binding sitei194ATPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi4 – 8ATPBy similarity5
    Nucleotide bindingi151 – 158ATPBy similarity8

    GO - Molecular functioni

    • 5-formyltetrahydrofolate cyclo-ligase activity Source: SGD
    • ATP binding Source: UniProtKB-KW

    GO - Biological processi

    Keywordsi

    Molecular functionLigase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YER183C-MONOMER
    ReactomeiR-SCE-196757 Metabolism of folate and pterines
    SABIO-RKiP40099

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
    Alternative name(s):
    5,10-methenyl-tetrahydrofolate synthetase
    Short name:
    MTHFS
    Short name:
    Methenyl-THF synthetase
    Gene namesi
    Name:FAU1
    Ordered Locus Names:YER183C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome V

    Organism-specific databases

    EuPathDBiFungiDB:YER183C
    SGDiS000000985 FAU1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Abolishes methenyltetrahydrofolate synthase activity and leads to accumulation of folinic acid. Leads to a striking methionine deficiency when combined with ADE16 and ADE17, 2 isoenzymes involved in the purine synthesis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002002801 – 2115-formyltetrahydrofolate cyclo-ligaseAdd BLAST211

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP40099
    PaxDbiP40099
    PRIDEiP40099

    Interactioni

    Protein-protein interaction databases

    BioGridi36937, 39 interactors
    IntActiP40099, 2 interactors
    MINTiP40099
    STRINGi4932.YER183C

    Structurei

    3D structure databases

    ProteinModelPortaliP40099
    SMRiP40099
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni152 – 156Substrate bindingBy similarity5

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000017791
    HOGENOMiHOG000007299
    InParanoidiP40099
    KOiK01934
    OMAiSTIYPCQ
    OrthoDBiEOG092C4TVL

    Family and domain databases

    Gene3Di3.40.50.10420, 1 hit
    InterProiView protein in InterPro
    IPR002698 FTHF_cligase
    IPR024185 FTHF_cligase-like_sf
    IPR037171 NagB/RpiA_transferase-like
    PANTHERiPTHR23407:SF1 PTHR23407:SF1, 1 hit
    PfamiView protein in Pfam
    PF01812 5-FTHF_cyc-lig, 1 hit
    PIRSFiPIRSF006806 FTHF_cligase, 1 hit
    SUPFAMiSSF100950 SSF100950, 1 hit
    TIGRFAMsiTIGR02727 MTHFS_bact, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P40099-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATKQLLRRQ IKRVINALDY DIIAAESHTI SQAVRSLIAS ANSRRVACYM
    60 70 80 90 100
    SMDKGEVTTG EIIKNLFQDG QEVFLPRCTH TSESKHFKLR EDHHPHLIFH
    110 120 130 140 150
    RMSSLKMVRD LKPQGPYQLK EPEPHIEESD ILDVVLVPGV AFDIKTGARM
    160 170 180 190 200
    GHGAGYYDDF FQRYKILHEG QKPLLVGLCL MEQVASPIPL EKHDYSMDCI
    210
    VCGDGSIHWF Q
    Length:211
    Mass (Da):24,059
    Last modified:February 1, 1995 - v1
    Checksum:i96F0D84B3B69DD57
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18922 Genomic DNA Translation: AAB64710.1
    BK006939 Genomic DNA Translation: DAA07846.1
    PIRiS50686
    RefSeqiNP_011110.1, NM_001179073.1

    Genome annotation databases

    EnsemblFungiiYER183C; YER183C; YER183C
    GeneIDi856932
    KEGGisce:YER183C

    Similar proteinsi

    Entry informationi

    Entry nameiFTHC_YEAST
    AccessioniPrimary (citable) accession number: P40099
    Secondary accession number(s): D3DM92
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: May 23, 2018
    This is version 135 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
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    Main funding by: National Institutes of Health