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P40099

- FTHC_YEAST

UniProt

P40099 - FTHC_YEAST

Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

FAU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Only enzyme known to utilize 5-formyltetrahydrofolate (folinic acid) as substrate. Contributes to tetrahydrofolate metabolism in an alternative way of folate biosynthesis. May regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids.1 Publication

    Catalytic activityi

    ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

    Kineticsi

    1. KM=43 µM for ATP1 Publication
    2. KM=33 µM for 5-formyltetrahydrofolate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561SubstrateBy similarity
    Binding sitei194 – 1941ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi4 – 85ATPBy similarity
    Nucleotide bindingi151 – 1588ATPBy similarity

    GO - Molecular functioni

    1. 5-formyltetrahydrofolate cyclo-ligase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YER183C-MONOMER.
    SABIO-RKP40099.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
    Alternative name(s):
    5,10-methenyl-tetrahydrofolate synthetase
    Short name:
    MTHFS
    Short name:
    Methenyl-THF synthetase
    Gene namesi
    Name:FAU1
    Ordered Locus Names:YER183C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER183c.
    SGDiS000000985. FAU1.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Abolishes methenyltetrahydrofolate synthase activity and leads to accumulation of folinic acid. Leads to a striking methionine deficiency when combined with ADE16 and ADE17, 2 isoenzymes involved in the purine synthesis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2112115-formyltetrahydrofolate cyclo-ligasePRO_0000200280Add
    BLAST

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP40099.
    PaxDbiP40099.

    Expressioni

    Gene expression databases

    GenevestigatoriP40099.

    Interactioni

    Protein-protein interaction databases

    BioGridi36937. 17 interactions.
    IntActiP40099. 1 interaction.
    MINTiMINT-4484715.
    STRINGi4932.YER183C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40099.
    SMRiP40099. Positions 26-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni152 – 1565Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0212.
    GeneTreeiENSGT00390000017791.
    HOGENOMiHOG000007299.
    KOiK01934.
    OMAiSCYLSMP.
    OrthoDBiEOG7FBRWF.

    Family and domain databases

    Gene3Di3.40.50.10420. 1 hit.
    InterProiIPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view]
    PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
    PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
    TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40099-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATKQLLRRQ IKRVINALDY DIIAAESHTI SQAVRSLIAS ANSRRVACYM    50
    SMDKGEVTTG EIIKNLFQDG QEVFLPRCTH TSESKHFKLR EDHHPHLIFH 100
    RMSSLKMVRD LKPQGPYQLK EPEPHIEESD ILDVVLVPGV AFDIKTGARM 150
    GHGAGYYDDF FQRYKILHEG QKPLLVGLCL MEQVASPIPL EKHDYSMDCI 200
    VCGDGSIHWF Q 211
    Length:211
    Mass (Da):24,059
    Last modified:February 1, 1995 - v1
    Checksum:i96F0D84B3B69DD57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18922 Genomic DNA. Translation: AAB64710.1.
    BK006939 Genomic DNA. Translation: DAA07846.1.
    PIRiS50686.
    RefSeqiNP_011110.1. NM_001179073.1.

    Genome annotation databases

    EnsemblFungiiYER183C; YER183C; YER183C.
    GeneIDi856932.
    KEGGisce:YER183C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18922 Genomic DNA. Translation: AAB64710.1 .
    BK006939 Genomic DNA. Translation: DAA07846.1 .
    PIRi S50686.
    RefSeqi NP_011110.1. NM_001179073.1.

    3D structure databases

    ProteinModelPortali P40099.
    SMRi P40099. Positions 26-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36937. 17 interactions.
    IntActi P40099. 1 interaction.
    MINTi MINT-4484715.
    STRINGi 4932.YER183C.

    Proteomic databases

    MaxQBi P40099.
    PaxDbi P40099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER183C ; YER183C ; YER183C .
    GeneIDi 856932.
    KEGGi sce:YER183C.

    Organism-specific databases

    CYGDi YER183c.
    SGDi S000000985. FAU1.

    Phylogenomic databases

    eggNOGi COG0212.
    GeneTreei ENSGT00390000017791.
    HOGENOMi HOG000007299.
    KOi K01934.
    OMAi SCYLSMP.
    OrthoDBi EOG7FBRWF.

    Enzyme and pathway databases

    BioCyci YEAST:YER183C-MONOMER.
    SABIO-RK P40099.

    Miscellaneous databases

    NextBioi 983412.
    PROi P40099.

    Gene expression databases

    Genevestigatori P40099.

    Family and domain databases

    Gene3Di 3.40.50.10420. 1 hit.
    InterProi IPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view ]
    PANTHERi PTHR23407:SF1. PTHR23407:SF1. 1 hit.
    Pfami PF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006806. FTHF_cligase. 1 hit.
    TIGRFAMsi TIGR02727. MTHFS_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Cloning and characterization of methenyltetrahydrofolate synthetase from Saccharomyces cerevisiae."
      Holmes W.B., Appling D.R.
      J. Biol. Chem. 277:20205-20213(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    6. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
      Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
      Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFTHC_YEAST
    AccessioniPrimary (citable) accession number: P40099
    Secondary accession number(s): D3DM92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 468 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3