Protein PEA2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40091 (PEA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein PEA2

Alternative name(s):
Protein PPF2

Gene names

Name:	PEA2
Synonyms:	PPF2
Ordered Locus Names:	YER149C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Localized to sites of polarized growth and is required for efficient mating and bipolar budding.
Miscellaneous	Present with 7820 molecules/cell in log phase SD medium.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Traceable author statement PubMed 10639324. Source: SGD actin filament organization Traceable author statement PubMed 10639324. Source: SGD bipolar cellular bud site selection Traceable author statement PubMed 10652251. Source: SGD pseudohyphal growth Inferred from mutant phenotype PubMed 9055077. Source: SGD regulation of initiation of mating projection growth Inferred from mutant phenotype PubMed 14734532. Source: SGD regulation of termination of mating projection growth Inferred from mutant phenotype PubMed 14734532. Source: SGD
Cellular_component	actin cap Traceable author statement PubMed 10652251. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD polarisome Inferred from direct assay PubMed 9632790. Source: SGD
Molecular_function	cytoskeletal regulatory protein binding Traceable author statement PubMed 10639324. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YMR124W	P39523	3	EBI-13106,EBI-27256

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

Protein PEA2

PRO_0000058300

Amino acid modifications

Modified residue

150

Phosphothreonine Ref.9

Modified residue

224

Phosphothreonine Ref.9

Modified residue

225

Phosphoserine Ref.9

Modified residue

226

Phosphoserine Ref.6 Ref.9

Modified residue

230

Phosphoserine Ref.6 Ref.8 Ref.9

Modified residue

345

Phosphoserine Ref.9

Natural variations

Natural variant

H → R in strain: CLIB 219. Ref.3

Natural variant

117

M → T in strain: CLIB 630. Ref.3

Natural variant

157

K → T in strain: CLIB 219. Ref.3

Natural variant

161

G → S in strain: CLIB 630. Ref.3

Natural variant

165

T → I in strain: CLIB 630. Ref.3

Natural variant

171

D → N in strain: CLIB 630. Ref.3

Natural variant

189

Q → L in strain: CLIB 382. Ref.3

Natural variant

210

I → M in strain: CLIB 413 haplotype Ha2. Ref.3

Natural variant

249

E → K in strain: CLIB 556. Ref.3

Natural variant

253

S → R in strain: Sigma 1278B. Ref.3

Natural variant

325

S → N in strain: CLIB 219. Ref.3

Natural variant

355

G → S in strain: R12. Ref.3

Natural variant

384

S → L in strain: CLIB 219. Ref.3

Natural variant

409

L → M in strain: CLIB 410, CLIB 413 and Sigma 1278B. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P40091 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 594B9A14E71A943D
Show »

FASTA

420

48,165

        10         20         30         40         50         60 
MHKFDLELSR RANPLLFSAE RYEEYPLKYD ELKQYLLSQN PSHPHHNSRP YTSIDYFDYL 

        70         80         90        100        110        120 
LYRSKNDESE IDLDKKLVSE FALYYVQKEH LNSDDLNPTL NELLKLQPKS ADWYEMMLRI 

       130        140        150        160        170        180 
LESINTTGID QLTKENNNSF PNSKRARSST NMGGTDKFNK GAYHTDKADD DKNEILQELT 

       190        200        210        220        230        240 
SFLMSNSIQK GIDIKPIPLD DPVKFLKNGI NSILDTCVSL EKNTSSPPIS PNAAAIQEED 

       250        260        270        280        290        300 
SSKKLEELET AFSDLQLAHN FLTKQFENDR AEYVQDIEKL TRTNRELQDK LLNNHSNLSK 

       310        320        330        340        350        360 
TEKKLHELEQ ENKELEKANN KLNSSRHNFG MSSPASSPVT WDPSSPSSVG SPTSGSGSRS 

       370        380        390        400        410        420 
LSIMTSEFKK VLTSTQRKYE KELSDEREHR FKLERELALL KNAEANTSLA LNRDDPPDML

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding." Valtz N.L.M., Herskowitz I. J. Cell Biol. 135:725-739(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A novel yeast gene necessary for pheromone-induced pointed projection formation." Yorihuzi T., Ohsumi Y. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[3]	"Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny." Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L. Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-90; THR-117; THR-157; SER-161; ILE-165; ASN-171; LEU-189; MET-210; LYS-249; ARG-253; ASN-325; SER-355; LEU-384 AND MET-409. Strain: CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12 and YIIc17.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226 AND SER-230, MASS SPECTROMETRY. Strain: 2124.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-150; THR-224; SER-225; SER-226; SER-230 AND SER-345, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07594 Genomic DNA. Translation: CAA68869.1. D38413 Genomic DNA. Translation: BAA07465.1. AJ585620 Genomic DNA. Translation: CAE52140.1. AJ585621 Genomic DNA. Translation: CAE52141.1. AJ585622 Genomic DNA. Translation: CAE52142.1. AJ585623 Genomic DNA. Translation: CAE52143.1. AJ585624 Genomic DNA. Translation: CAE52144.1. AJ585625 Genomic DNA. Translation: CAE52145.1. AJ585626 Genomic DNA. Translation: CAE52146.1. AJ585627 Genomic DNA. Translation: CAE52147.1. AJ585628 Genomic DNA. Translation: CAE52148.1. AJ585629 Genomic DNA. Translation: CAE52149.1. AJ585630 Genomic DNA. Translation: CAE52150.1. AJ585631 Genomic DNA. Translation: CAE52151.1. AJ585632 Genomic DNA. Translation: CAE52152.1. AJ585633 Genomic DNA. Translation: CAE52153.1. AJ585634 Genomic DNA. Translation: CAE52154.1. AJ585635 Genomic DNA. Translation: CAE52155.1. AJ585636 Genomic DNA. Translation: CAE52156.1. U18917 Genomic DNA. Translation: AAB64676.1. BK006939 Genomic DNA. Translation: DAA07810.1.
PIR	S50652.
RefSeq	NP_011076.1. NM_001179039.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2463N.
IntAct	P40091. 4 interactions.
MINT	MINT-436009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER149C; YER149C; YER149C.
GeneID	856892.
KEGG	sce:YER149C.
Organism-specific databases
CYGD	YER149c.
SGD	S000000951. PEA2.
Phylogenomic databases
OMA	FLTKQFE.
OrthoDB	EOG46QB31.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30310-MONOMER.
Gene expression databases
Genevestigator	P40091.
GermOnline	YER149C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	983299.

Entry information

Entry name

PEA2_YEAST

Accession

Primary (citable) accession number: P40091
Secondary accession number(s): D3DM56

Q70DD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents