P40090 (SCC4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAU2 chromatid cohesion factor homolog

Alternative name(s):
Sister chromatid cohesion protein 4

Gene names

Name:	SCC4
Ordered Locus Names:	YER147C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes. Ref.3
Subunit structure	Interacts with SCC2 to form the cohesin loading complex. Ref.3
Subcellular location	Nucleus. Note: Associates with chromatin.
Miscellaneous	Present with 907 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SCC4/mau-2 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis
Cellular component	Nucleus
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair Inferred from mutant phenotype PubMed 15610742. Source: SGD establishment of protein localization to chromatin Inferred from mutant phenotype Ref.3. Source: SGD mitotic sister chromatid cohesion Inferred from mutant phenotype Ref.3. Source: SGD rDNA condensation Inferred from mutant phenotype PubMed 18708580. Source: SGD
Cellular_component	SMC loading complex Inferred from physical interaction Ref.3. Source: SGD nuclear chromatin Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SCC2	Q04002	5	EBI-16679,EBI-16662

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	MAU2 chromatid cohesion factor homolog		PRO_0000193217

Sequences

	Sequence		Length	Mass (Da)
	P40090 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1269D931C656E608	FASTA	624	72,141
	10 20 30 40 50 60 MENLGDKLSI SQVYHLAQEY RDHAYSIANK IGSEEGLKQY YGLMNMSIQM FQLLKTKCTL 70 80 90 100 110 120 SVLEDSKVTF EMVELLIQET YNFDLAELYI SSLKERLQTH QSDTDLVEEI MRCEFLLLHD 130 140 150 160 170 180 LPLMRDSKFH YKIALRNCNE LVQYMVNLQD ELYQNWASVF QYVGVMLCIK LKQHRRVKTS 190 200 210 220 230 240 FHGLLSQCRE KSQWKWFLNL CYVNYLLNER FPIPEDALQE LRSTELHTVG PELYAWKLAL 250 260 270 280 290 300 EMVIQLCKDG NITDHLNEFK NFFDTNKQSL VTNEGKGCVI KIMPRIALKV ELPMIFHYKE 310 320 330 340 350 360 LKNILLLLQS VSYIVNCYDE KGNFSRKFLP KVYSTTQKLI KNIAAGGVSM NELDSRIQTY 370 380 390 400 410 420 KSILEFCEFY KVWEQTLLKG AVVTTESPKL GPSPGYVRLL QAMKVQFEGG GAVEEYTRLA 430 440 450 460 470 480 QSGGTSSEVK MISLLNCYTV QAARVSRCSG DKQGELVEQC NKVWLQVEKL LQETDLQFNP 490 500 510 520 530 540 IWECTVTILW LFSHFEPFSW NPLPCSDKQR AEYVSKLREF YSSNKFVAGE AVADNRFKLK 550 560 570 580 590 600 KALLLQILVN YLGGRMLEHD LGEIYAISAK CFDMCRQQGG MRKVQYVIGI WHLMNCTVAM 610 620 RGKDVALTNA KLEALVKQIT SVKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins." Ciosk R., Shirayama M., Shevchenko A., Tanaka T., Toth A., Shevchenko A., Nasmyth K. Mol. Cell 5:243-254(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 390-398, FUNCTION, INTERACTION WITH SCC2.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18917 Genomic DNA. Translation: AAB64674.1. BK006939 Genomic DNA. Translation: DAA07808.1.
PIR	S50650.
RefSeq	NP_011074.3. NM_001179037.3.
3D structure databases
ProteinModelPortal	P40090.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5627N.
IntAct	P40090. 3 interactions.
MINT	MINT-555872.
STRING	4932.YER147C.
Proteomic databases
PaxDb	P40090.
PeptideAtlas	P40090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER147C; YER147C; YER147C.
GeneID	856890.
KEGG	sce:YER147C. sce:YER151C.
Organism-specific databases
CYGD	YER147c.
SGD	S000000949. SCC4.
Phylogenomic databases
eggNOG	NOG39193.
HOGENOM	HOG000066106.
KO	K06673. K11841.
OMA	GIWHLMN.
OrthoDB	EOG418FXD.
Gene expression databases
Genevestigator	P40090.
GermOnline	YER147C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019440. Cohesin_loading_factor. [Graphical view]
Pfam	PF10345. Cohesin_load. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983293.

Entry information

Entry name

SCC4_YEAST

Accession

Primary (citable) accession number: P40090
Secondary accession number(s): D3DM54

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program