Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA damage-inducible protein 1

Gene

DDI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Aspartic protease (PubMed:21266539). Appears to act as negative regulator of constitutive exocytosis (PubMed:10330187, PubMed:12051757). May act at the level of secretory vesicle docking and fusion as a competitive inhibitor of SNARE assembly (PubMed:12925750). Acts as a linker between the 19S proteasome and polyubiquitinated proteins like the HO endonuclease and UFO1 via UBA domain interactions with ubiquitin for their subsequent degradation (PubMed:17144915, PubMed:16478980). Required for S-phase checkpoint control (PubMed:11238935, PubMed:17144915).1 Publication7 Publications

Miscellaneous

Present with 6510 molecules/cell in log phase SD medium.1 Publication

Enzyme regulationi

Inhibited by the proteinase inhibitors indinavir, lopinavir, nelfinavir, isovaleryl pepstatin, ritonavir, saquinavir and tipranavir.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei220Curated1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB-KW
  • SNARE binding Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • protein secretion Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
  • vesicle-mediated transport Source: SGD

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30304-MONOMER

Protein family/group databases

MEROPSiA28.001

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-inducible protein 1 (EC:3.4.23.-By similarity)
Alternative name(s):
v-SNARE-master 1
Gene namesi
Name:DDI1
Synonyms:VSM1
Ordered Locus Names:YER143W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER143W
SGDiS000000945 DDI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi220D → A: Increased protein secretion most likely due to reduced catalytic activity. Cells are larger and clump together. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109971 – 428DNA damage-inducible protein 1Add BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki171Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki257Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP40087
PaxDbiP40087
PRIDEiP40087

PTM databases

iPTMnetiP40087

Expressioni

Inductioni

By DNA damage via PDR3.4 Publications

Interactioni

Subunit structurei

Forms homodimers. Interacts with RAD23. These interactions are mediated by the UBA domain. Is also able to bind ubiquitin and polyubiquitinated proteins. Interacts with the SNAREs SNC1, SNC2, SSO1, TLG1 and TLG2. Binding to SSO1 is promoted by the phosphorylation of 'Ser-49' of SSO1 by TKP1.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • SNARE binding Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi36892, 105 interactors
DIPiDIP-1216N
IntActiP40087, 6 interactors
MINTiP40087
STRINGi4932.YER143W

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Turni8 – 10Combined sources3
Beta strandi13 – 17Combined sources5
Helixi25 – 34Combined sources10
Helixi40 – 42Combined sources3
Beta strandi44 – 47Combined sources4
Beta strandi50 – 52Combined sources3
Helixi60 – 63Combined sources4
Beta strandi70 – 74Combined sources5
Turni76 – 79Combined sources4
Helixi90 – 103Combined sources14
Helixi105 – 114Combined sources10
Helixi118 – 123Combined sources6
Helixi125 – 130Combined sources6
Helixi133 – 140Combined sources8
Helixi155 – 163Combined sources9
Helixi168 – 189Combined sources22
Beta strandi205 – 210Combined sources6
Beta strandi213 – 219Combined sources7
Beta strandi227 – 229Combined sources3
Helixi230 – 235Combined sources6
Helixi239 – 241Combined sources3
Beta strandi260 – 269Combined sources10
Beta strandi272 – 281Combined sources10
Beta strandi286 – 289Combined sources4
Helixi291 – 296Combined sources6
Beta strandi300 – 302Combined sources3
Turni303 – 306Combined sources4
Beta strandi307 – 310Combined sources4
Beta strandi313 – 316Combined sources4
Helixi320 – 322Combined sources3
Helixi392 – 399Combined sources8
Turni400 – 402Combined sources3
Helixi405 – 415Combined sources11
Helixi419 – 426Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I1AX-ray2.30A/B/C/D180-325[»]
2MR9NMR-A389-428[»]
2MRONMR-B389-428[»]
2MRPNMR-A2-80[»]
2MWSNMR-B2-80[»]
2N7ENMR-A1-80[»]
4Z2ZX-ray1.80A/B185-325[»]
5KESNMR-A86-196[»]
ProteinModelPortaliP40087
SMRiP40087
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40087

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 80Ubiquitin-likeAdd BLAST80
Domaini389 – 428UBAPROSITE-ProRule annotationAdd BLAST40

Sequence similaritiesi

Belongs to the DDI1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000047125
HOGENOMiHOG000234736
InParanoidiP40087
KOiK11885
OMAiNQARISE
OrthoDBiEOG092C4Q00

Family and domain databases

CDDicd05479 RP_DDI, 1 hit
Gene3Di2.40.70.10, 1 hit
InterProiView protein in InterPro
IPR033322 DDI1
IPR001995 Peptidase_A2_cat
IPR019103 Peptidase_aspartic_DDI1-type
IPR021109 Peptidase_aspartic_dom_sf
IPR015940 UBA
IPR009060 UBA-like_sf
PANTHERiPTHR12917:SF1 PTHR12917:SF1, 1 hit
PfamiView protein in Pfam
PF09668 Asp_protease, 1 hit
PF00627 UBA, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit

Sequencei

Sequence statusi: Complete.

P40087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLTISNELT GEIYGPIEVS EDMALTDLIA LLQADCGFDK TKHDLYYNMD
60 70 80 90 100
ILDSNRTQSL KELGLKTDDL LLIRGKISNS IQTDAATLSD EAFIEQFRQE
110 120 130 140 150
LLNNQMLRSQ LILQIPGLND LVNDPLLFRE RLGPLILQRR YGGYNTAMNP
160 170 180 190 200
FGIPQDEYTR LMANPDDPDN KKRIAELLDQ QAIDEQLRNA IEYTPEMFTQ
210 220 230 240 250
VPMLYINIEI NNYPVKAFVD TGAQTTIMST RLAKKTGLSR MIDKRFIGEA
260 270 280 290 300
RGVGTGKIIG RIHQAQVKIE TQYIPCSFTV LDTDIDVLIG LDMLKRHLAC
310 320 330 340 350
VDLKENVLRI AEVETSFLSE AEIPKSFQEG LPAPTSVTTS SDKPLTPTKT
360 370 380 390 400
SSTLPPQPGA VPALAPRTGM GPTPTGRSTA GATTATGRTF PEQTIKQLMD
410 420
LGFPRDAVVK ALKQTNGNAE FAASLLFQ
Length:428
Mass (Da):47,354
Last modified:February 1, 1995 - v1
Checksum:iB43425B029B7658B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14002 Genomic DNA Translation: AAB82066.1
AF034895 Genomic DNA Translation: AAC18522.1
U18917 Genomic DNA Translation: AAB64670.1
AY692945 Genomic DNA Translation: AAT92964.1
BK006939 Genomic DNA Translation: DAA07804.1
PIRiS50646
RefSeqiNP_011070.3, NM_001179033.3

Genome annotation databases

EnsemblFungiiYER143W; YER143W; YER143W
GeneIDi856886
KEGGisce:YER143W

Similar proteinsi

Entry informationi

Entry nameiDDI1_YEAST
AccessioniPrimary (citable) accession number: P40087
Secondary accession number(s): D3DM50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health