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Protein

Endoplasmic reticulum membrane protein 65

Gene

EMP65

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in membrane protein folding.1 Publication

GO - Biological processi

  • protein folding in endoplasmic reticulum Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30301-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum membrane protein 651 Publication
Alternative name(s):
65 kDa endoplasmic reticulum membrane protein1 Publication
Gene namesi
Name:EMP651 Publication
Ordered Locus Names:YER140WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER140W.
SGDiS000000942. EMP65.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 87Cytoplasmic1 PublicationAdd BLAST87
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 151Lumenal1 PublicationAdd BLAST43
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Topological domaini173 – 224Cytoplasmic1 PublicationAdd BLAST52
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Topological domaini246 – 330Lumenal1 PublicationAdd BLAST85
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352 – 391Cytoplasmic1 PublicationAdd BLAST40
Transmembranei392 – 412HelicalSequence analysisAdd BLAST21
Topological domaini413 – 428Lumenal1 PublicationAdd BLAST16
Transmembranei429 – 449HelicalSequence analysisAdd BLAST21
Topological domaini450 – 556Cytoplasmic1 PublicationAdd BLAST107

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: SGD
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026541 – 556Endoplasmic reticulum membrane protein 65Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineCombined sources1
Glycosylationi318N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP40085.

PTM databases

iPTMnetiP40085.

Interactioni

Subunit structurei

Interacts with SLP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SLP1Q122323EBI-22717,EBI-35990

Protein-protein interaction databases

BioGridi36889. 62 interactors.
DIPiDIP-5409N.
IntActiP40085. 2 interactors.
MINTiMINT-521141.

Structurei

3D structure databases

ProteinModelPortaliP40085.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TAPT1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000010628.
HOGENOMiHOG000066104.
InParanoidiP40085.
OMAiTKFNRIR.
OrthoDBiEOG092C3JIJ.

Family and domain databases

InterProiIPR008010. Tatp1.
[Graphical view]
PANTHERiPTHR13317. PTHR13317. 1 hit.
PfamiPF05346. DUF747. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHKDTAVAK DTAKKRLLRR NSAPSAIHII SRLDKKWSFL WNTIDRHNIV
60 70 80 90 100
EEQDESSAAK SEEEHEDDYE LEQLLNMIRI PMFLEKFMLF ALLTSLDCFL
110 120 130 140 150
YYFTVLPIRL IKGYVKQFKS YRQHYRLQQR SGHKNKIPFR YRITSREYKE
160 170 180 190 200
RCMIFIIVIS SILLSKLDTS KLYHRIKRQS TMKLYMLFSV LEMADKMLAS
210 220 230 240 250
LGQSLLTVML SRKNSERILL HKCLLVSMSL TYVTIHGYVL VYQAISLNIA
260 270 280 290 300
VNSYSNALLT LLLSMQFAEI KSSVLKKFDK EGFFQITIAD VVERFKLTLL
310 320 330 340 350
LSITGLRNLQ SWSSSLSNTS INFWSPRSTL SIVINILCGP MVSVVGSEVL
360 370 380 390 400
VDWAKHAYIT KFNRIRPQIY DKFYYIIYKD YSTRTHKLED RLGLPLPAFV
410 420 430 440 450
VLFIVMVRPT LFKSSEPSYL PSLFRILFMG ASVFLLALLA KFTLDLILIK
460 470 480 490 500
WSKRIEQRFR DQAFNTVVTE EEYVPGLLSG GMGKVDVSTR IALHSDYNKE
510 520 530 540 550
NRIETESVSP MRKRKTTLTA ECTPPSLNDI RRQKDSKNPR SLENVARYKM

VSKRIW
Length:556
Mass (Da):64,794
Last modified:February 1, 1995 - v1
Checksum:i16B04343A0F33AAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64667.1.
BK006939 Genomic DNA. Translation: DAA07801.1.
PIRiS50643.
RefSeqiNP_011067.3. NM_001179030.3.

Genome annotation databases

EnsemblFungiiYER140W; YER140W; YER140W.
GeneIDi856883.
KEGGisce:YER140W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64667.1.
BK006939 Genomic DNA. Translation: DAA07801.1.
PIRiS50643.
RefSeqiNP_011067.3. NM_001179030.3.

3D structure databases

ProteinModelPortaliP40085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36889. 62 interactors.
DIPiDIP-5409N.
IntActiP40085. 2 interactors.
MINTiMINT-521141.

PTM databases

iPTMnetiP40085.

Proteomic databases

PRIDEiP40085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER140W; YER140W; YER140W.
GeneIDi856883.
KEGGisce:YER140W.

Organism-specific databases

EuPathDBiFungiDB:YER140W.
SGDiS000000942. EMP65.

Phylogenomic databases

GeneTreeiENSGT00390000010628.
HOGENOMiHOG000066104.
InParanoidiP40085.
OMAiTKFNRIR.
OrthoDBiEOG092C3JIJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30301-MONOMER.

Miscellaneous databases

PROiP40085.

Family and domain databases

InterProiIPR008010. Tatp1.
[Graphical view]
PANTHERiPTHR13317. PTHR13317. 1 hit.
PfamiPF05346. DUF747. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAPT1_YEAST
AccessioniPrimary (citable) accession number: P40085
Secondary accession number(s): D3DM47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.