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Protein

Endoplasmic reticulum membrane protein 65

Gene

EMP65

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in membrane protein folding.1 Publication

GO - Biological processi

  1. protein folding in endoplasmic reticulum Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30301-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum membrane protein 651 Publication
Alternative name(s):
65 kDa endoplasmic reticulum membrane protein1 Publication
Gene namesi
Name:EMP651 Publication
Ordered Locus Names:YER140WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER140w.
SGDiS000000942. EMP65.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence Analysis. Mitochondrion 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8787Cytoplasmic1 PublicationAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 15143Lumenal1 PublicationAdd
BLAST
Transmembranei152 – 17221HelicalSequence AnalysisAdd
BLAST
Topological domaini173 – 22452Cytoplasmic1 PublicationAdd
BLAST
Transmembranei225 – 24521HelicalSequence AnalysisAdd
BLAST
Topological domaini246 – 33085Lumenal1 PublicationAdd
BLAST
Transmembranei331 – 35121HelicalSequence AnalysisAdd
BLAST
Topological domaini352 – 39140Cytoplasmic1 PublicationAdd
BLAST
Transmembranei392 – 41221HelicalSequence AnalysisAdd
BLAST
Topological domaini413 – 42816Lumenal1 PublicationAdd
BLAST
Transmembranei429 – 44921HelicalSequence AnalysisAdd
BLAST
Topological domaini450 – 556107Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: SGD
  2. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Endoplasmic reticulum membrane protein 65PRO_0000202654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Phosphoserine2 Publications
Glycosylationi318 – 3181N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein, Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP40085.

Interactioni

Subunit structurei

Interacts with SLP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SLP1Q122321EBI-22717,EBI-35990

Protein-protein interaction databases

BioGridi36889. 62 interactions.
DIPiDIP-5409N.
IntActiP40085. 2 interactions.
MINTiMINT-521141.
STRINGi4932.YER140W.

Structurei

3D structure databases

ProteinModelPortaliP40085.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TAPT1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG241124.
GeneTreeiENSGT00390000010628.
HOGENOMiHOG000066104.
InParanoidiP40085.
OMAiRYKMVSK.
OrthoDBiEOG71VT4B.

Family and domain databases

InterProiIPR008010. Membrane_Tatp1/CMV_rcpt.
[Graphical view]
PANTHERiPTHR13317. PTHR13317. 1 hit.
PfamiPF05346. DUF747. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHKDTAVAK DTAKKRLLRR NSAPSAIHII SRLDKKWSFL WNTIDRHNIV
60 70 80 90 100
EEQDESSAAK SEEEHEDDYE LEQLLNMIRI PMFLEKFMLF ALLTSLDCFL
110 120 130 140 150
YYFTVLPIRL IKGYVKQFKS YRQHYRLQQR SGHKNKIPFR YRITSREYKE
160 170 180 190 200
RCMIFIIVIS SILLSKLDTS KLYHRIKRQS TMKLYMLFSV LEMADKMLAS
210 220 230 240 250
LGQSLLTVML SRKNSERILL HKCLLVSMSL TYVTIHGYVL VYQAISLNIA
260 270 280 290 300
VNSYSNALLT LLLSMQFAEI KSSVLKKFDK EGFFQITIAD VVERFKLTLL
310 320 330 340 350
LSITGLRNLQ SWSSSLSNTS INFWSPRSTL SIVINILCGP MVSVVGSEVL
360 370 380 390 400
VDWAKHAYIT KFNRIRPQIY DKFYYIIYKD YSTRTHKLED RLGLPLPAFV
410 420 430 440 450
VLFIVMVRPT LFKSSEPSYL PSLFRILFMG ASVFLLALLA KFTLDLILIK
460 470 480 490 500
WSKRIEQRFR DQAFNTVVTE EEYVPGLLSG GMGKVDVSTR IALHSDYNKE
510 520 530 540 550
NRIETESVSP MRKRKTTLTA ECTPPSLNDI RRQKDSKNPR SLENVARYKM

VSKRIW
Length:556
Mass (Da):64,794
Last modified:February 1, 1995 - v1
Checksum:i16B04343A0F33AAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64667.1.
BK006939 Genomic DNA. Translation: DAA07801.1.
PIRiS50643.
RefSeqiNP_011067.3. NM_001179030.3.

Genome annotation databases

EnsemblFungiiYER140W; YER140W; YER140W.
GeneIDi856883.
KEGGisce:YER140W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64667.1.
BK006939 Genomic DNA. Translation: DAA07801.1.
PIRiS50643.
RefSeqiNP_011067.3. NM_001179030.3.

3D structure databases

ProteinModelPortaliP40085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36889. 62 interactions.
DIPiDIP-5409N.
IntActiP40085. 2 interactions.
MINTiMINT-521141.
STRINGi4932.YER140W.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER140W; YER140W; YER140W.
GeneIDi856883.
KEGGisce:YER140W.

Organism-specific databases

CYGDiYER140w.
SGDiS000000942. EMP65.

Phylogenomic databases

eggNOGiNOG241124.
GeneTreeiENSGT00390000010628.
HOGENOMiHOG000066104.
InParanoidiP40085.
OMAiRYKMVSK.
OrthoDBiEOG71VT4B.

Enzyme and pathway databases

BioCyciYEAST:G3O-30301-MONOMER.

Miscellaneous databases

NextBioi983272.
PROiP40085.

Gene expression databases

GenevestigatoriP40085.

Family and domain databases

InterProiIPR008010. Membrane_Tatp1/CMV_rcpt.
[Graphical view]
PANTHERiPTHR13317. PTHR13317. 1 hit.
PfamiPF05346. DUF747. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum."
    Jonikas M.C., Collins S.R., Denic V., Oh E., Quan E.M., Schmid V., Weibezahn J., Schwappach B., Walter P., Weissman J.S., Schuldiner M.
    Science 323:1693-1697(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Genetic analysis of Mps3 SUN domain mutants in Saccharomyces cerevisiae reveals an interaction with the SUN-like protein Slp1."
    Friederichs J.M., Gardner J.M., Smoyer C.J., Whetstine C.R., Gogol M., Slaughter B.D., Jaspersen S.L.
    G3 (Bethesda) 2:1703-1718(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SLP1.

Entry informationi

Entry nameiTAPT1_YEAST
AccessioniPrimary (citable) accession number: P40085
Secondary accession number(s): D3DM47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.