P40085 (TAPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TAPT1 homolog

Gene names

Ordered Locus Names:

YER140W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Ref.3.
Sequence similarities	Belongs to the TAPT1 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding in endoplasmic reticulum Inferred from genetic interaction PubMed 19325107. Source: SGD
Cellular_component	integral to endoplasmic reticulum membrane Inferred from sequence model PubMed 23275891. Source: SGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.3PubMed 16823961. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SLP1	Q12232	1	EBI-22717,EBI-35990

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Protein TAPT1 homolog		PRO_0000202654
Regions
	Topological domain	1 – 87	87	Cytoplasmic Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Topological domain	109 – 151	43	Mitochondrial intermembrane Potential
	Transmembrane	152 – 172	21	Helical; Potential
	Topological domain	173 – 224	52	Cytoplasmic Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Topological domain	246 – 330	85	Mitochondrial intermembrane Potential
	Transmembrane	331 – 351	21	Helical; Potential
	Topological domain	352 – 391	40	Cytoplasmic Potential
	Transmembrane	392 – 412	21	Helical; Potential
	Topological domain	413 – 428	16	Mitochondrial intermembrane Potential
	Transmembrane	429 – 449	21	Helical; Potential
	Topological domain	450 – 556	107	Cytoplasmic Potential
Amino acid modifications
	Modified residue	22	1	Phosphoserine Ref.5 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P40085 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 16B04343A0F33AAF	FASTA	556	64,794
	10 20 30 40 50 60 MQHKDTAVAK DTAKKRLLRR NSAPSAIHII SRLDKKWSFL WNTIDRHNIV EEQDESSAAK 70 80 90 100 110 120 SEEEHEDDYE LEQLLNMIRI PMFLEKFMLF ALLTSLDCFL YYFTVLPIRL IKGYVKQFKS 130 140 150 160 170 180 YRQHYRLQQR SGHKNKIPFR YRITSREYKE RCMIFIIVIS SILLSKLDTS KLYHRIKRQS 190 200 210 220 230 240 TMKLYMLFSV LEMADKMLAS LGQSLLTVML SRKNSERILL HKCLLVSMSL TYVTIHGYVL 250 260 270 280 290 300 VYQAISLNIA VNSYSNALLT LLLSMQFAEI KSSVLKKFDK EGFFQITIAD VVERFKLTLL 310 320 330 340 350 360 LSITGLRNLQ SWSSSLSNTS INFWSPRSTL SIVINILCGP MVSVVGSEVL VDWAKHAYIT 370 380 390 400 410 420 KFNRIRPQIY DKFYYIIYKD YSTRTHKLED RLGLPLPAFV VLFIVMVRPT LFKSSEPSYL 430 440 450 460 470 480 PSLFRILFMG ASVFLLALLA KFTLDLILIK WSKRIEQRFR DQAFNTVVTE EEYVPGLLSG 490 500 510 520 530 540 GMGKVDVSTR IALHSDYNKE NRIETESVSP MRKRKTTLTA ECTPPSLNDI RRQKDSKNPR 550 SLENVARYKM VSKRIW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18917 Genomic DNA. Translation: AAB64667.1. BK006939 Genomic DNA. Translation: DAA07801.1.
PIR	S50643.
RefSeq	NP_011067.3. NM_001179030.3.
3D structure databases
ProteinModelPortal	P40085.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5409N.
IntAct	P40085. 2 interactions.
MINT	MINT-521141.
STRING	4932.YER140W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER140W; YER140W; YER140W.
GeneID	856883.
KEGG	sce:YER140W. sce:YER144C.
Organism-specific databases
CYGD	YER140w.
SGD	S000000942. YER140W.
Phylogenomic databases
eggNOG	NOG241124.
GeneTree	ENSGT00390000010628.
HOGENOM	HOG000066104.
KO	K11839.
OMA	RYKMVSK.
OrthoDB	EOG40S3QH.
Gene expression databases
Genevestigator	P40085.
GermOnline	YER140W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008010. Membrane_Tatp1/CMV_rcpt. [Graphical view]
PANTHER	PTHR13317. PTHR13317. 1 hit.
Pfam	PF05346. DUF747. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983272.

Entry information

Entry name

TAPT1_YEAST

Accession

Primary (citable) accession number: P40085
Secondary accession number(s): D3DM47

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program