##gff-version 3
P40073	UniProtKB	Chain	1	367	.	.	.	ID=PRO_0000072231;Note=High osmolarity signaling protein SHO1	
P40073	UniProtKB	Topological domain	1	32	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Transmembrane	33	53	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Topological domain	54	65	.	.	.	Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Transmembrane	66	86	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Topological domain	87	93	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Transmembrane	94	114	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Topological domain	115	122	.	.	.	Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Transmembrane	123	143	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Topological domain	144	367	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Domain	300	361	.	.	.	Note=SH3;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00192	
P40073	UniProtKB	Region	252	276	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P40073	UniProtKB	Modified residue	166	166	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17363249;Dbxref=PMID:17363249	
P40073	UniProtKB	Glycosylation	59	59	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
P40073	UniProtKB	Mutagenesis	166	166	.	.	.	Note=Diminishes the formation of oligomers%2C dampens activation of the HOG1 kinase%2C and impairs growth in high-salt or sorbitol conditions. S->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17363249;Dbxref=PMID:17363249	
P40073	UniProtKB	Mutagenesis	309	309	.	.	.	Note=Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15200958;Dbxref=PMID:15200958	
P40073	UniProtKB	Mutagenesis	317	317	.	.	.	Note=Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. D->I%2CH;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15200958;Dbxref=PMID:15200958	
P40073	UniProtKB	Mutagenesis	355	355	.	.	.	Note=Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. Y->A%2CF%2CI%2CM;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15200958;Dbxref=PMID:15200958	
P40073	UniProtKB	Beta strand	302	309	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN	
P40073	UniProtKB	Beta strand	315	317	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN	
P40073	UniProtKB	Beta strand	328	332	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN	
P40073	UniProtKB	Beta strand	336	342	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN	
P40073	UniProtKB	Beta strand	348	352	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN	
P40073	UniProtKB	Helix	353	355	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN	
P40073	UniProtKB	Beta strand	356	363	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2VKN