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P40073

- SHO1_YEAST

UniProt

P40073 - SHO1_YEAST

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Protein

High osmolarity signaling protein SHO1

Gene

SHO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plasma membrane osmosensor that activates the high osmolarity glycerol (HOG) MAPK signaling pathway in response to high osmolarity. Detects changes in external osmolarity and activates PBS2 through the stimulation of STE11 and targets PBS2 to the plasma membrane. PBS2 activation leads to changes in glycerol production that helps to balance the intracellular and external osmotic pressures. Activates also HOG1 in response to heat stress and mediates resistance to oxidative stress. Involved in the regulation of the mating pathway. May be a receptor that feeds into the pseudohyphal growth pathway.20 Publications

GO - Molecular functioni

  1. MAP-kinase scaffold activity Source: SGD
  2. osmosensor activity Source: SGD

GO - Biological processi

  1. cellular response to heat Source: SGD
  2. establishment of cell polarity Source: SGD
  3. osmosensory signaling pathway Source: SGD
  4. positive regulation of signal transduction Source: GOC
  5. signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-30282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
High osmolarity signaling protein SHO1
Alternative name(s):
Osmosensor SHO1
Suppressor of SUA8-1 mutation
Synthetic high osmolarity-sensitive protein 1
Gene namesi
Name:SHO1
Synonyms:SSU81
Ordered Locus Names:YER118C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER118c.
SGDiS000000920. SHO1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Bud. Bud neck. Cell projection
Note: Localizes at the tip of the mating projection during conjugation.

GO - Cellular componenti

  1. cellular bud Source: SGD
  2. cellular bud neck Source: SGD
  3. HICS complex Source: SGD
  4. integral component of membrane Source: UniProtKB-KW
  5. mating projection tip Source: SGD
  6. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661S → E: Diminishes the formation of oligomers, dampens activation of the HOG1 kinase, and impairs growth in high-salt or sorbitol conditions. 1 Publication
Mutagenesisi309 – 3091Y → A: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication
Mutagenesisi317 – 3171D → I or H: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication
Mutagenesisi355 – 3551Y → A, F, I or M: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367High osmolarity signaling protein SHO1PRO_0000072231Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
Modified residuei166 – 1661Phosphoserine1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP40073.

Expressioni

Gene expression databases

GenevestigatoriP40073.

Interactioni

Subunit structurei

Forms homooligomers. Interacts (via the SH3 domain) with PBS2. Interacts with FUS1, STE11, STE50 and RNA polymerase II.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AAD14P428842EBI-18140,EBI-1994
BCH2P361222EBI-18140,EBI-26374
FUS1P117107EBI-18140,EBI-7179
GIP2P400362EBI-18140,EBI-7612
LAS17Q124465EBI-18140,EBI-10022
LCL3P531532EBI-18140,EBI-23857
PBS2P080185EBI-18140,EBI-12972
PCF11P390812EBI-18140,EBI-12980
RGA1P390832EBI-18140,EBI-15044
STE11P235613EBI-18140,EBI-18259
STE50P253443EBI-18140,EBI-18305
TUS1Q064123EBI-18140,EBI-37117

Protein-protein interaction databases

BioGridi36863. 135 interactions.
DIPiDIP-2472N.
IntActiP40073. 76 interactions.
MINTiMINT-518897.
STRINGi4932.YER118C.

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi302 – 3098
Beta strandi315 – 3173
Beta strandi328 – 3325
Beta strandi336 – 3427
Beta strandi348 – 3525
Helixi353 – 3553
Beta strandi356 – 3638

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VKNX-ray2.05A298-367[»]
ProteinModelPortaliP40073.
SMRiP40073. Positions 299-364.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40073.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini54 – 6512ExtracellularSequence AnalysisAdd
BLAST
Topological domaini87 – 937CytoplasmicSequence Analysis
Topological domaini115 – 1228ExtracellularSequence Analysis
Topological domaini144 – 367224CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei33 – 5321HelicalSequence AnalysisAdd
BLAST
Transmembranei66 – 8621HelicalSequence AnalysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence AnalysisAdd
BLAST
Transmembranei123 – 14321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini300 – 36162SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SHO1 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG44108.
HOGENOMiHOG000174182.
InParanoidiP40073.
KOiK11246.
OMAiDAYEISF.
OrthoDBiEOG7MPRR4.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40073-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSISSKIRPT PRKPSRMATD HSFKMKKFYA DPFAISSISL AIVSWVIAIG
60 70 80 90 100
GSISSASTNE SFPRFTWWGI VYQFLIICSL MLFYCFDLVD HYRIFITTSI
110 120 130 140 150
AVAFVYNTNS ATNLVYADGP KKAAASAGVI LLSIINLIWI LYYGGDNASP
160 170 180 190 200
TNRWIDSFSI KGIRPSPLEN SLHRARRRGN RNTTPYQNNV YNDAIRDSGY
210 220 230 240 250
ATQFDGYPQQ QPSHTNYVSS TALAGFENTQ PNTSEAVNLH LNTLQQRINS
260 270 280 290 300
ASNAKETNDN SNNQTNTNIG NTFDTDFSNG NTETTMGDTL GLYSDIGDDN
310 320 330 340 350
FIYKAKALYP YDADDDDAYE ISFEQNEILQ VSDIEGRWWK ARRANGETGI
360
IPSNYVQLID GPEEMHR
Length:367
Mass (Da):41,126
Last modified:February 1, 1995 - v1
Checksum:iE467A4D50AA3EDB6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15653 Genomic DNA. Translation: AAA61904.1.
L41926 Genomic DNA. Translation: AAC41664.1.
U18916 Genomic DNA. Translation: AAC03216.1.
BK006939 Genomic DNA. Translation: DAA07778.1.
PIRiS50621.
RefSeqiNP_011043.1. NM_001179008.1.

Genome annotation databases

EnsemblFungiiYER118C; YER118C; YER118C.
GeneIDi856854.
KEGGisce:YER118C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15653 Genomic DNA. Translation: AAA61904.1 .
L41926 Genomic DNA. Translation: AAC41664.1 .
U18916 Genomic DNA. Translation: AAC03216.1 .
BK006939 Genomic DNA. Translation: DAA07778.1 .
PIRi S50621.
RefSeqi NP_011043.1. NM_001179008.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VKN X-ray 2.05 A 298-367 [» ]
ProteinModelPortali P40073.
SMRi P40073. Positions 299-364.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36863. 135 interactions.
DIPi DIP-2472N.
IntActi P40073. 76 interactions.
MINTi MINT-518897.
STRINGi 4932.YER118C.

Proteomic databases

PaxDbi P40073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER118C ; YER118C ; YER118C .
GeneIDi 856854.
KEGGi sce:YER118C.

Organism-specific databases

CYGDi YER118c.
SGDi S000000920. SHO1.

Phylogenomic databases

eggNOGi NOG44108.
HOGENOMi HOG000174182.
InParanoidi P40073.
KOi K11246.
OMAi DAYEISF.
OrthoDBi EOG7MPRR4.

Enzyme and pathway databases

BioCyci YEAST:G3O-30282-MONOMER.

Miscellaneous databases

EvolutionaryTracei P40073.
NextBioi 983195.

Gene expression databases

Genevestigatori P40073.

Family and domain databases

InterProi IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Berroteran R.W., Hampsey M.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor."
    Maeda T., Takekawa M., Saito H.
    Science 269:554-558(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK."
    Posas F., Saito H.
    Science 276:1702-1705(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PBS2.
  6. "The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae."
    O'Rourke S.M., Herskowitz I.
    Genes Dev. 12:2874-2886(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway."
    Raitt D.C., Posas F., Saito H.
    EMBO J. 19:4623-4631(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PBS2.
  8. "The Saccharomyces cerevisiae Sln1p-Ssk1p two-component system mediates response to oxidative stress and in an oxidant-specific fashion."
    Singh K.K.
    Free Radic. Biol. Med. 29:1043-1050(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Calcofluor antifungal action depends on chitin and a functional high-osmolarity glycerol response (HOG) pathway: evidence for a physiological role of the Saccharomyces cerevisiae HOG pathway under noninducing conditions."
    Garcia-Rodriguez L.J., Duran A., Roncero C.
    J. Bacteriol. 182:2428-2437(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Response of Saccharomyces cerevisiae to severe osmotic stress: evidence for a novel activation mechanism of the HOG MAP kinase pathway."
    Van Wuytswinkel O., Reiser V., Siderius M., Kelders M.C., Ammerer G., Ruis H., Mager W.H.
    Mol. Microbiol. 37:382-397(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Polarized localization of yeast Pbs2 depends on osmostress, the membrane protein Sho1 and Cdc42."
    Reiser V., Salah S.M., Ammerer G.
    Nat. Cell Biol. 2:620-627(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae."
    Toh-e A., Oguchi T.
    Genes Genet. Syst. 76:393-410(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway, and protein tyrosine phosphatases are essential under heat stress."
    Winkler A., Arkind C., Mattison C.P., Burkholder A., Knoche K., Ota I.M.
    Eukaryot. Cell 1:163-173(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. Cited for: SUBCELLULAR LOCATION.
  15. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  16. "Optimization of specificity in a cellular protein interaction network by negative selection."
    Zarrinpar A., Park S.H., Lim W.A.
    Nature 426:676-680(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, INTERACTION WITH PBS2.
  17. "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms."
    Park S.H., Zarrinpar A., Lim W.A.
    Science 299:1061-1064(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast."
    Cullen P.J., Sabbagh W. Jr., Graham E., Irick M.M., van Olden E.K., Neal C., Delrow J., Bardwell L., Sprague G.F. Jr.
    Genes Dev. 18:1695-1708(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MSB2.
  19. "Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating."
    Nelson B., Parsons A.B., Evangelista M., Schaefer K., Kennedy K., Ritchie S., Petryshen T.L., Boone C.
    Genetics 166:67-77(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FUS1.
  20. "Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis."
    O'Rourke S.M., Herskowitz I.
    Mol. Biol. Cell 15:532-542(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  21. "Protein-protein interaction affinity plays a crucial role in controlling the Sho1p-mediated signal transduction pathway in yeast."
    Marles J.A., Dahesh S., Haynes J., Andrews B.J., Davidson A.R.
    Mol. Cell 14:813-823(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PBS2, DOMAIN, MUTAGENESIS OF TYR-309; ASP-317 AND TYR-355.
  22. "Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway."
    Zarrinpar A., Bhattacharyya R.P., Nittler M.P., Lim W.A.
    Mol. Cell 14:825-832(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STE11.
  23. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  24. "Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway."
    Tatebayashi K., Yamamoto K., Tanaka K., Tomida T., Maruoka T., Kasukawa E., Saito H.
    EMBO J. 25:3033-3044(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STE11 AND STE50.
  25. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  26. "A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway."
    Hao N., Behar M., Parnell S.C., Torres M.P., Borchers C.H., Elston T.C., Dohlman H.G.
    Curr. Biol. 17:659-667(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-166, MUTAGENESIS OF SER-166, SUBUNIT.
  27. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  28. Cited for: FUNCTION.
  29. "The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response."
    Pitoniak A., Birkaya B., Dionne H.M., Vadaie N., Cullen P.J.
    Mol. Biol. Cell 20:3101-3114(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  30. "Dynamic signaling in the Hog1 MAPK pathway relies on high basal signal transduction."
    Macia J., Regot S., Peeters T., Conde N., Sole R., Posas F.
    Sci. Signal. 2:RA13-RA13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  31. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  32. "Structural genomics of yeast SH3 domains."
    Kursula P., Kursula I., Song Y.H., Paraskevopoulos M., Wilmanns M.
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 298-367.

Entry informationi

Entry nameiSHO1_YEAST
AccessioniPrimary (citable) accession number: P40073
Secondary accession number(s): D3DM24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3