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Protein

High osmolarity signaling protein SHO1

Gene

SHO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane osmosensor that activates the high osmolarity glycerol (HOG) MAPK signaling pathway in response to high osmolarity. Detects changes in external osmolarity and activates PBS2 through the stimulation of STE11 and targets PBS2 to the plasma membrane. PBS2 activation leads to changes in glycerol production that helps to balance the intracellular and external osmotic pressures. Activates also HOG1 in response to heat stress and mediates resistance to oxidative stress. Involved in the regulation of the mating pathway. May be a receptor that feeds into the pseudohyphal growth pathway.20 Publications

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: SGD
  • osmosensor activity Source: SGD

GO - Biological processi

  • cellular response to heat Source: SGD
  • establishment of cell polarity Source: SGD
  • osmosensory signaling pathway Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-30282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
High osmolarity signaling protein SHO1
Alternative name(s):
Osmosensor SHO1
Suppressor of SUA8-1 mutation
Synthetic high osmolarity-sensitive protein 1
Gene namesi
Name:SHO1
Synonyms:SSU81
Ordered Locus Names:YER118C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER118C.
SGDiS000000920. SHO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232CytoplasmicSequence analysisAdd
BLAST
Transmembranei33 – 5321HelicalSequence analysisAdd
BLAST
Topological domaini54 – 6512ExtracellularSequence analysisAdd
BLAST
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Topological domaini87 – 937CytoplasmicSequence analysis
Transmembranei94 – 11421HelicalSequence analysisAdd
BLAST
Topological domaini115 – 1228ExtracellularSequence analysis
Transmembranei123 – 14321HelicalSequence analysisAdd
BLAST
Topological domaini144 – 367224CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cellular bud Source: SGD
  • cellular bud neck Source: SGD
  • HICS complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • mating projection tip Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi166 – 1661S → E: Diminishes the formation of oligomers, dampens activation of the HOG1 kinase, and impairs growth in high-salt or sorbitol conditions. 1 Publication
Mutagenesisi309 – 3091Y → A: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication
Mutagenesisi317 – 3171D → I or H: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication
Mutagenesisi355 – 3551Y → A, F, I or M: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367High osmolarity signaling protein SHO1PRO_0000072231Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence analysis
Modified residuei166 – 1661Phosphoserine1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

PTM databases

iPTMnetiP40073.

Interactioni

Subunit structurei

Forms homooligomers. Interacts (via the SH3 domain) with PBS2. Interacts with FUS1, STE11, STE50 and RNA polymerase II.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AAD14P428842EBI-18140,EBI-1994
BCH2P361222EBI-18140,EBI-26374
FUS1P117107EBI-18140,EBI-7179
GIP2P400362EBI-18140,EBI-7612
LAS17Q124465EBI-18140,EBI-10022
LCL3P531532EBI-18140,EBI-23857
PBS2P080185EBI-18140,EBI-12972
PCF11P390812EBI-18140,EBI-12980
RGA1P390832EBI-18140,EBI-15044
STE11P235613EBI-18140,EBI-18259
STE50P253443EBI-18140,EBI-18305
TUS1Q064123EBI-18140,EBI-37117

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi36863. 135 interactions.
DIPiDIP-2472N.
IntActiP40073. 76 interactions.
MINTiMINT-518897.

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi302 – 3098Combined sources
Beta strandi315 – 3173Combined sources
Beta strandi328 – 3325Combined sources
Beta strandi336 – 3427Combined sources
Beta strandi348 – 3525Combined sources
Helixi353 – 3553Combined sources
Beta strandi356 – 3638Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VKNX-ray2.05A298-367[»]
ProteinModelPortaliP40073.
SMRiP40073. Positions 299-364.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40073.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini300 – 36162SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SHO1 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000174182.
InParanoidiP40073.
KOiK11246.
OMAiDAYEISF.
OrthoDBiEOG092C3BG5.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISSKIRPT PRKPSRMATD HSFKMKKFYA DPFAISSISL AIVSWVIAIG
60 70 80 90 100
GSISSASTNE SFPRFTWWGI VYQFLIICSL MLFYCFDLVD HYRIFITTSI
110 120 130 140 150
AVAFVYNTNS ATNLVYADGP KKAAASAGVI LLSIINLIWI LYYGGDNASP
160 170 180 190 200
TNRWIDSFSI KGIRPSPLEN SLHRARRRGN RNTTPYQNNV YNDAIRDSGY
210 220 230 240 250
ATQFDGYPQQ QPSHTNYVSS TALAGFENTQ PNTSEAVNLH LNTLQQRINS
260 270 280 290 300
ASNAKETNDN SNNQTNTNIG NTFDTDFSNG NTETTMGDTL GLYSDIGDDN
310 320 330 340 350
FIYKAKALYP YDADDDDAYE ISFEQNEILQ VSDIEGRWWK ARRANGETGI
360
IPSNYVQLID GPEEMHR
Length:367
Mass (Da):41,126
Last modified:February 1, 1995 - v1
Checksum:iE467A4D50AA3EDB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15653 Genomic DNA. Translation: AAA61904.1.
L41926 Genomic DNA. Translation: AAC41664.1.
U18916 Genomic DNA. Translation: AAC03216.1.
BK006939 Genomic DNA. Translation: DAA07778.1.
PIRiS50621.
RefSeqiNP_011043.1. NM_001179008.1.

Genome annotation databases

EnsemblFungiiYER118C; YER118C; YER118C.
GeneIDi856854.
KEGGisce:YER118C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15653 Genomic DNA. Translation: AAA61904.1.
L41926 Genomic DNA. Translation: AAC41664.1.
U18916 Genomic DNA. Translation: AAC03216.1.
BK006939 Genomic DNA. Translation: DAA07778.1.
PIRiS50621.
RefSeqiNP_011043.1. NM_001179008.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VKNX-ray2.05A298-367[»]
ProteinModelPortaliP40073.
SMRiP40073. Positions 299-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36863. 135 interactions.
DIPiDIP-2472N.
IntActiP40073. 76 interactions.
MINTiMINT-518897.

PTM databases

iPTMnetiP40073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER118C; YER118C; YER118C.
GeneIDi856854.
KEGGisce:YER118C.

Organism-specific databases

EuPathDBiFungiDB:YER118C.
SGDiS000000920. SHO1.

Phylogenomic databases

HOGENOMiHOG000174182.
InParanoidiP40073.
KOiK11246.
OMAiDAYEISF.
OrthoDBiEOG092C3BG5.

Enzyme and pathway databases

BioCyciYEAST:G3O-30282-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40073.
PROiP40073.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHO1_YEAST
AccessioniPrimary (citable) accession number: P40073
Secondary accession number(s): D3DM24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.