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Protein

High osmolarity signaling protein SHO1

Gene

SHO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane osmosensor that activates the high osmolarity glycerol (HOG) MAPK signaling pathway in response to high osmolarity. Detects changes in external osmolarity and activates PBS2 through the stimulation of STE11 and targets PBS2 to the plasma membrane. PBS2 activation leads to changes in glycerol production that helps to balance the intracellular and external osmotic pressures. Activates also HOG1 in response to heat stress and mediates resistance to oxidative stress. Involved in the regulation of the mating pathway. May be a receptor that feeds into the pseudohyphal growth pathway.20 Publications

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: SGD
  • osmosensor activity Source: SGD

GO - Biological processi

  • cellular response to heat Source: SGD
  • establishment of cell polarity Source: SGD
  • osmosensory signaling pathway Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-30282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
High osmolarity signaling protein SHO1
Alternative name(s):
Osmosensor SHO1
Suppressor of SUA8-1 mutation
Synthetic high osmolarity-sensitive protein 1
Gene namesi
Name:SHO1
Synonyms:SSU81
Ordered Locus Names:YER118C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER118C.
SGDiS000000920. SHO1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicSequence analysisAdd BLAST32
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54 – 65ExtracellularSequence analysisAdd BLAST12
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Topological domaini87 – 93CytoplasmicSequence analysis7
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 122ExtracellularSequence analysis8
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 367CytoplasmicSequence analysisAdd BLAST224

GO - Cellular componenti

  • cellular bud Source: SGD
  • cellular bud neck Source: SGD
  • HICS complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • mating projection tip Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166S → E: Diminishes the formation of oligomers, dampens activation of the HOG1 kinase, and impairs growth in high-salt or sorbitol conditions. 1 Publication1
Mutagenesisi309Y → A: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication1
Mutagenesisi317D → I or H: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication1
Mutagenesisi355Y → A, F, I or M: Decreases the interaction with PBS2 and leads to decreased HOG pathway response and increased aberrant mating pathway activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000722311 – 367High osmolarity signaling protein SHO1Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...)Sequence analysis1
Modified residuei166Phosphoserine1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP40073.

PTM databases

iPTMnetiP40073.

Interactioni

Subunit structurei

Forms homooligomers. Interacts (via the SH3 domain) with PBS2. Interacts with FUS1, STE11, STE50 and RNA polymerase II.9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AAD14P428842EBI-18140,EBI-1994
BCH2P361222EBI-18140,EBI-26374
FUS1P117107EBI-18140,EBI-7179
GIP2P400362EBI-18140,EBI-7612
LAS17Q124465EBI-18140,EBI-10022
LCL3P531532EBI-18140,EBI-23857
PBS2P080185EBI-18140,EBI-12972
PCF11P390812EBI-18140,EBI-12980
RGA1P390832EBI-18140,EBI-15044
STE11P235613EBI-18140,EBI-18259
STE50P253443EBI-18140,EBI-18305
TUS1Q064123EBI-18140,EBI-37117

GO - Molecular functioni

  • MAP-kinase scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi36863. 135 interactors.
DIPiDIP-2472N.
IntActiP40073. 76 interactors.
MINTiMINT-518897.

Structurei

Secondary structure

1367
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi302 – 309Combined sources8
Beta strandi315 – 317Combined sources3
Beta strandi328 – 332Combined sources5
Beta strandi336 – 342Combined sources7
Beta strandi348 – 352Combined sources5
Helixi353 – 355Combined sources3
Beta strandi356 – 363Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VKNX-ray2.05A298-367[»]
ProteinModelPortaliP40073.
SMRiP40073.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40073.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 361SH3PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Belongs to the SHO1 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000174182.
InParanoidiP40073.
KOiK11246.
OMAiDAYEISF.
OrthoDBiEOG092C3BG5.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISSKIRPT PRKPSRMATD HSFKMKKFYA DPFAISSISL AIVSWVIAIG
60 70 80 90 100
GSISSASTNE SFPRFTWWGI VYQFLIICSL MLFYCFDLVD HYRIFITTSI
110 120 130 140 150
AVAFVYNTNS ATNLVYADGP KKAAASAGVI LLSIINLIWI LYYGGDNASP
160 170 180 190 200
TNRWIDSFSI KGIRPSPLEN SLHRARRRGN RNTTPYQNNV YNDAIRDSGY
210 220 230 240 250
ATQFDGYPQQ QPSHTNYVSS TALAGFENTQ PNTSEAVNLH LNTLQQRINS
260 270 280 290 300
ASNAKETNDN SNNQTNTNIG NTFDTDFSNG NTETTMGDTL GLYSDIGDDN
310 320 330 340 350
FIYKAKALYP YDADDDDAYE ISFEQNEILQ VSDIEGRWWK ARRANGETGI
360
IPSNYVQLID GPEEMHR
Length:367
Mass (Da):41,126
Last modified:February 1, 1995 - v1
Checksum:iE467A4D50AA3EDB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15653 Genomic DNA. Translation: AAA61904.1.
L41926 Genomic DNA. Translation: AAC41664.1.
U18916 Genomic DNA. Translation: AAC03216.1.
BK006939 Genomic DNA. Translation: DAA07778.1.
PIRiS50621.
RefSeqiNP_011043.1. NM_001179008.1.

Genome annotation databases

EnsemblFungiiYER118C; YER118C; YER118C.
GeneIDi856854.
KEGGisce:YER118C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15653 Genomic DNA. Translation: AAA61904.1.
L41926 Genomic DNA. Translation: AAC41664.1.
U18916 Genomic DNA. Translation: AAC03216.1.
BK006939 Genomic DNA. Translation: DAA07778.1.
PIRiS50621.
RefSeqiNP_011043.1. NM_001179008.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VKNX-ray2.05A298-367[»]
ProteinModelPortaliP40073.
SMRiP40073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36863. 135 interactors.
DIPiDIP-2472N.
IntActiP40073. 76 interactors.
MINTiMINT-518897.

PTM databases

iPTMnetiP40073.

Proteomic databases

PRIDEiP40073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER118C; YER118C; YER118C.
GeneIDi856854.
KEGGisce:YER118C.

Organism-specific databases

EuPathDBiFungiDB:YER118C.
SGDiS000000920. SHO1.

Phylogenomic databases

HOGENOMiHOG000174182.
InParanoidiP40073.
KOiK11246.
OMAiDAYEISF.
OrthoDBiEOG092C3BG5.

Enzyme and pathway databases

BioCyciYEAST:G3O-30282-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40073.
PROiP40073.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHO1_YEAST
AccessioniPrimary (citable) accession number: P40073
Secondary accession number(s): D3DM24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.