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P40072

- SLX8_YEAST

UniProt

P40072 - SLX8_YEAST

Protein

E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8

Gene

SLX8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mediates ubiquitination and subsequent desumoylation/degradation of sumoylated proteins and proteins containing SUMO-like domains. Promotes UBC4-dependent ubiquitination that mediates the proteolytic down-regulation of sumoylated proteins. Involved in the stimulation of ubiquitin conjugating enzymes, including UBC1, UBC4, UBC5 and UBC13-MMS2. Acts as a critical suppressor of gross chromosomal rearrangements (GCRs) during normal cell cycle progression. Has a role in localizing the DNA damage protein DCD2. Involved in stabilizing, restarting or resolving transiently stalled replication forks. Prevents accumulation of DNA damage during cell cycle progression. Inhibits RAD51-independent recombination via modulating the sumoylation of DNA repair proteins.10 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri206 – 25045RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. ubiquitin-protein transferase activity Source: SGD
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: SGD
    2. DNA repair Source: UniProtKB-KW
    3. protein sumoylation Source: SGD
    4. protein ubiquitination Source: SGD
    5. telomere maintenance Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30280-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8 (EC:6.3.2.-)
    Alternative name(s):
    Synthetic lethal of unknown function protein 8
    Gene namesi
    Name:SLX8
    Ordered Locus Names:YER116C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER116c.
    SGDiS000000918. SLX8.

    Subcellular locationi

    Nucleusnucleolus 2 Publications
    Note: Localizes at the replication centers.

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell
    2. nucleus Source: SGD
    3. SUMO-targeted ubiquitin ligase complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 274274E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8PRO_0000056336Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501Phosphoserine3 Publications
    Modified residuei66 – 661Phosphothreonine2 Publications
    Modified residuei67 – 671Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP40072.

    Interactioni

    Subunit structurei

    Heterodimer. Interacts with SLX5/HEX3.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SLX5P328282EBI-22661,EBI-8276

    Protein-protein interaction databases

    BioGridi36861. 320 interactions.
    DIPiDIP-1365N.
    IntActiP40072. 4 interactions.
    MINTiMINT-396089.
    STRINGi4932.YER116C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40072.
    SMRiP40072. Positions 202-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri206 – 25045RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5574.
    OrthoDBiEOG7H1K05.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF00097. zf-C3HC4. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40072-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARRPDNQNP EGENLRIKRV RLESVRQNDE EEENEVSRTQ NIVTDNRHDS    50
    PEAVVEIIGE RALENTSEED GDDDLSLFRA LEEDPGSDHN TSNNDSGNHD 100
    RETMHTEEPE ASSGNNITLT NNVEELHTMD VLSQTANTPS ASPMLDAAPP 150
    TTKPGTNSKE QTVDLTADAI DLDAEEQQVL QISDDDFQEE TKEAPKEYGA 200
    AKDYRCPICF EPPETALMTL CGHVFCCPCL FQMVNSSRTC RQFGHCALCR 250
    SKVYLKDVRL IILRKKQVKK KVKS 274
    Length:274
    Mass (Da):30,764
    Last modified:February 1, 1995 - v1
    Checksum:i358DB6D137155DC1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18916 Genomic DNA. Translation: AAC03214.1.
    BK006939 Genomic DNA. Translation: DAA07776.1.
    PIRiS50619.
    RefSeqiNP_011041.3. NM_001179006.3.

    Genome annotation databases

    EnsemblFungiiYER116C; YER116C; YER116C.
    GeneIDi856852.
    KEGGisce:YER116C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18916 Genomic DNA. Translation: AAC03214.1 .
    BK006939 Genomic DNA. Translation: DAA07776.1 .
    PIRi S50619.
    RefSeqi NP_011041.3. NM_001179006.3.

    3D structure databases

    ProteinModelPortali P40072.
    SMRi P40072. Positions 202-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36861. 320 interactions.
    DIPi DIP-1365N.
    IntActi P40072. 4 interactions.
    MINTi MINT-396089.
    STRINGi 4932.YER116C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER116C ; YER116C ; YER116C .
    GeneIDi 856852.
    KEGGi sce:YER116C.

    Organism-specific databases

    CYGDi YER116c.
    SGDi S000000918. SLX8.

    Phylogenomic databases

    eggNOGi COG5574.
    OrthoDBi EOG7H1K05.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-30280-MONOMER.

    Miscellaneous databases

    NextBioi 983189.
    PROi P40072.

    Gene expression databases

    Genevestigatori P40072.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    Pfami PF00097. zf-C3HC4. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae."
      Mullen J.R., Kaliraman V., Ibrahim S.S., Brill S.J.
      Genetics 157:103-118(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, INTERACTION WITH SLX5.
    4. "Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae."
      Zhang C., Roberts T.M., Yang J., Desai R., Brown G.W.
      DNA Repair 5:336-346(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN STABILIZATION OF DNA DAMAGE.
    5. "Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae."
      Wang Z., Jones G.M., Prelich G.
      Genetics 172:1499-1509(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN STABILIZATION OF DNA DAMAGE.
    6. "The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity."
      Ii T., Fung J., Mullen J.R., Brill S.J.
      Cell Cycle 6:2800-2809(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITIN-PROTEIN LIGASE ACTIVITY, FUNCTION IN STIMULATION OF UBIQUITIN CONJUGASTING ENZYMES, INTERACTION WITH SLX5/HEX3.
    7. "Stimulation of in vitro sumoylation by Slx5-Slx8: evidence for a functional interaction with the SUMO pathway."
      Ii T., Mullen J.R., Slagle C.E., Brill S.J.
      DNA Repair 6:1679-1691(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, INTERACTION WITH SLX5/HEX3.
    8. Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, LIGASE ACTIVITY, INTERACTION WITH SLX5.
    9. "The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation."
      Xie Y., Kerscher O., Kroetz M.B., McConchie H.F., Sung P., Hochstrasser M.
      J. Biol. Chem. 282:34176-34184(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, LIGASE ACTIVITY, INTERACTION WITH SLX5.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50; THR-66 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "The Slx5-Slx8 complex affects sumoylation of DNA repair proteins and negatively regulates recombination."
      Burgess R.C., Rahman S., Lisby M., Rothstein R., Zhao X.
      Mol. Cell. Biol. 27:6153-6162(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEGATIVE REGULATION OF RECOMBINATION, SUBCELLULAR LOCATION.
    12. "A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae."
      Andersen M.P., Nelson Z.W., Hetrick E.D., Gottschling D.E.
      Genetics 179:1179-1195(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DELETION ANALYSIS.
    13. "Activation of the Slx5-Slx8 ubiquitin ligase by poly-small ubiquitin-like modifier conjugates."
      Mullen J.R., Brill S.J.
      J. Biol. Chem. 283:19912-19921(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase."
      Nagai S., Dubrana K., Tsai-Pflugfelder M., Davidson M.B., Roberts T.M., Brown G.W., Varela E., Hediger F., Gasser S.M., Krogan N.J.
      Science 322:597-602(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, INTERACTION WITH SLX5/HEX3, SUBCELLULAR LOCATION.
    16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50; THR-66 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSLX8_YEAST
    AccessioniPrimary (citable) accession number: P40072
    Secondary accession number(s): D3DM22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3