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P40072

- SLX8_YEAST

UniProt

P40072 - SLX8_YEAST

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Protein

E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8

Gene

SLX8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates ubiquitination and subsequent desumoylation/degradation of sumoylated proteins and proteins containing SUMO-like domains. Promotes UBC4-dependent ubiquitination that mediates the proteolytic down-regulation of sumoylated proteins. Involved in the stimulation of ubiquitin conjugating enzymes, including UBC1, UBC4, UBC5 and UBC13-MMS2. Acts as a critical suppressor of gross chromosomal rearrangements (GCRs) during normal cell cycle progression. Has a role in localizing the DNA damage protein DCD2. Involved in stabilizing, restarting or resolving transiently stalled replication forks. Prevents accumulation of DNA damage during cell cycle progression. Inhibits RAD51-independent recombination via modulating the sumoylation of DNA repair proteins.10 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri206 – 25045RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: SGD
  2. DNA repair Source: UniProtKB-KW
  3. protein sumoylation Source: SGD
  4. protein ubiquitination Source: SGD
  5. telomere maintenance Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30280-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8 (EC:6.3.2.-)
Alternative name(s):
Synthetic lethal of unknown function protein 8
Gene namesi
Name:SLX8
Ordered Locus Names:YER116C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER116c.
SGDiS000000918. SLX8.

Subcellular locationi

Nucleusnucleolus 2 Publications
Note: Localizes at the replication centers.

GO - Cellular componenti

  1. nucleus Source: SGD
  2. SUMO-targeted ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8PRO_0000056336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501Phosphoserine3 Publications
Modified residuei66 – 661Phosphothreonine2 Publications
Modified residuei67 – 671Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Expressioni

Gene expression databases

GenevestigatoriP40072.

Interactioni

Subunit structurei

Heterodimer. Interacts with SLX5/HEX3.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SLX5P328282EBI-22661,EBI-8276

Protein-protein interaction databases

BioGridi36861. 322 interactions.
DIPiDIP-1365N.
IntActiP40072. 4 interactions.
MINTiMINT-396089.
STRINGi4932.YER116C.

Structurei

3D structure databases

ProteinModelPortaliP40072.
SMRiP40072. Positions 200-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri206 – 25045RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5574.
InParanoidiP40072.
OrthoDBiEOG7H1K05.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40072-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARRPDNQNP EGENLRIKRV RLESVRQNDE EEENEVSRTQ NIVTDNRHDS
60 70 80 90 100
PEAVVEIIGE RALENTSEED GDDDLSLFRA LEEDPGSDHN TSNNDSGNHD
110 120 130 140 150
RETMHTEEPE ASSGNNITLT NNVEELHTMD VLSQTANTPS ASPMLDAAPP
160 170 180 190 200
TTKPGTNSKE QTVDLTADAI DLDAEEQQVL QISDDDFQEE TKEAPKEYGA
210 220 230 240 250
AKDYRCPICF EPPETALMTL CGHVFCCPCL FQMVNSSRTC RQFGHCALCR
260 270
SKVYLKDVRL IILRKKQVKK KVKS
Length:274
Mass (Da):30,764
Last modified:February 1, 1995 - v1
Checksum:i358DB6D137155DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03214.1.
BK006939 Genomic DNA. Translation: DAA07776.1.
PIRiS50619.
RefSeqiNP_011041.3. NM_001179006.3.

Genome annotation databases

EnsemblFungiiYER116C; YER116C; YER116C.
GeneIDi856852.
KEGGisce:YER116C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03214.1 .
BK006939 Genomic DNA. Translation: DAA07776.1 .
PIRi S50619.
RefSeqi NP_011041.3. NM_001179006.3.

3D structure databases

ProteinModelPortali P40072.
SMRi P40072. Positions 200-263.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36861. 322 interactions.
DIPi DIP-1365N.
IntActi P40072. 4 interactions.
MINTi MINT-396089.
STRINGi 4932.YER116C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER116C ; YER116C ; YER116C .
GeneIDi 856852.
KEGGi sce:YER116C.

Organism-specific databases

CYGDi YER116c.
SGDi S000000918. SLX8.

Phylogenomic databases

eggNOGi COG5574.
InParanoidi P40072.
OrthoDBi EOG7H1K05.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci YEAST:G3O-30280-MONOMER.

Miscellaneous databases

NextBioi 983189.
PROi P40072.

Gene expression databases

Genevestigatori P40072.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
Pfami PF00097. zf-C3HC4. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae."
    Mullen J.R., Kaliraman V., Ibrahim S.S., Brill S.J.
    Genetics 157:103-118(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, INTERACTION WITH SLX5.
  4. "Suppression of genomic instability by SLX5 and SLX8 in Saccharomyces cerevisiae."
    Zhang C., Roberts T.M., Yang J., Desai R., Brown G.W.
    DNA Repair 5:336-346(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN STABILIZATION OF DNA DAMAGE.
  5. "Genetic analysis connects SLX5 and SLX8 to the SUMO pathway in Saccharomyces cerevisiae."
    Wang Z., Jones G.M., Prelich G.
    Genetics 172:1499-1509(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN STABILIZATION OF DNA DAMAGE.
  6. "The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity."
    Ii T., Fung J., Mullen J.R., Brill S.J.
    Cell Cycle 6:2800-2809(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITIN-PROTEIN LIGASE ACTIVITY, FUNCTION IN STIMULATION OF UBIQUITIN CONJUGASTING ENZYMES, INTERACTION WITH SLX5/HEX3.
  7. "Stimulation of in vitro sumoylation by Slx5-Slx8: evidence for a functional interaction with the SUMO pathway."
    Ii T., Mullen J.R., Slagle C.E., Brill S.J.
    DNA Repair 6:1679-1691(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, INTERACTION WITH SLX5/HEX3.
  8. Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, LIGASE ACTIVITY, INTERACTION WITH SLX5.
  9. "The yeast Hex3.Slx8 heterodimer is a ubiquitin ligase stimulated by substrate sumoylation."
    Xie Y., Kerscher O., Kroetz M.B., McConchie H.F., Sung P., Hochstrasser M.
    J. Biol. Chem. 282:34176-34184(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, LIGASE ACTIVITY, INTERACTION WITH SLX5.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50; THR-66 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "The Slx5-Slx8 complex affects sumoylation of DNA repair proteins and negatively regulates recombination."
    Burgess R.C., Rahman S., Lisby M., Rothstein R., Zhao X.
    Mol. Cell. Biol. 27:6153-6162(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEGATIVE REGULATION OF RECOMBINATION, SUBCELLULAR LOCATION.
  12. "A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae."
    Andersen M.P., Nelson Z.W., Hetrick E.D., Gottschling D.E.
    Genetics 179:1179-1195(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DELETION ANALYSIS.
  13. "Activation of the Slx5-Slx8 ubiquitin ligase by poly-small ubiquitin-like modifier conjugates."
    Mullen J.R., Brill S.J.
    J. Biol. Chem. 283:19912-19921(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase."
    Nagai S., Dubrana K., Tsai-Pflugfelder M., Davidson M.B., Roberts T.M., Brown G.W., Varela E., Hediger F., Gasser S.M., Krogan N.J.
    Science 322:597-602(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEGRADATION OF SUMOYLATED PROTEINS, INTERACTION WITH SLX5/HEX3, SUBCELLULAR LOCATION.
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50; THR-66 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSLX8_YEAST
AccessioniPrimary (citable) accession number: P40072
Secondary accession number(s): D3DM22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3