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Protein

E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8

Gene

SLX8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates ubiquitination and subsequent desumoylation/degradation of sumoylated proteins and proteins containing SUMO-like domains. Promotes UBC4-dependent ubiquitination that mediates the proteolytic down-regulation of sumoylated proteins. Involved in the stimulation of ubiquitin conjugating enzymes, including UBC1, UBC4, UBC5 and UBC13-MMS2. Acts as a critical suppressor of gross chromosomal rearrangements (GCRs) during normal cell cycle progression. Has a role in localizing the DNA damage protein DCD2. Involved in stabilizing, restarting or resolving transiently stalled replication forks. Prevents accumulation of DNA damage during cell cycle progression. Inhibits RAD51-independent recombination via modulating the sumoylation of DNA repair proteins.10 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri206 – 250RING-typePROSITE-ProRule annotationAdd BLAST45

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: UniProtKB-KW
  • negative regulation of protein sumoylation Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein sumoylation Source: SGD
  • protein ubiquitination Source: SGD
  • telomere maintenance Source: SGD

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30280-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase SLX8Curated
Synthetic lethal of unknown function protein 8
Gene namesi
Name:SLX8
Ordered Locus Names:YER116C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER116C.
SGDiS000000918. SLX8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563361 – 274E3 ubiquitin-protein ligase complex SLX5-SLX8 subunit SLX8Add BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1
Modified residuei66PhosphothreonineCombined sources1
Modified residuei67PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP40072.

PTM databases

iPTMnetiP40072.

Interactioni

Subunit structurei

Heterodimer. Interacts with SLX5/HEX3.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SLX5P328282EBI-22661,EBI-8276

Protein-protein interaction databases

BioGridi36861. 497 interactors.
DIPiDIP-1365N.
IntActiP40072. 5 interactors.
MINTiMINT-396089.
STRINGi4932.YER116C.

Structurei

3D structure databases

ProteinModelPortaliP40072.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri206 – 250RING-typePROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiP40072.
OrthoDBiEOG092C386W.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF00097. zf-C3HC4. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P40072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRPDNQNP EGENLRIKRV RLESVRQNDE EEENEVSRTQ NIVTDNRHDS
60 70 80 90 100
PEAVVEIIGE RALENTSEED GDDDLSLFRA LEEDPGSDHN TSNNDSGNHD
110 120 130 140 150
RETMHTEEPE ASSGNNITLT NNVEELHTMD VLSQTANTPS ASPMLDAAPP
160 170 180 190 200
TTKPGTNSKE QTVDLTADAI DLDAEEQQVL QISDDDFQEE TKEAPKEYGA
210 220 230 240 250
AKDYRCPICF EPPETALMTL CGHVFCCPCL FQMVNSSRTC RQFGHCALCR
260 270
SKVYLKDVRL IILRKKQVKK KVKS
Length:274
Mass (Da):30,764
Last modified:February 1, 1995 - v1
Checksum:i358DB6D137155DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03214.1.
BK006939 Genomic DNA. Translation: DAA07776.1.
PIRiS50619.
RefSeqiNP_011041.3. NM_001179006.3.

Genome annotation databases

EnsemblFungiiYER116C; YER116C; YER116C.
GeneIDi856852.
KEGGisce:YER116C.

Similar proteinsi

Entry informationi

Entry nameiSLX8_YEAST
AccessioniPrimary (citable) accession number: P40072
Secondary accession number(s): D3DM22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names