Importin subunit beta-4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Importin subunit beta-4

Alternative name(s):
Importin-123
Karyopherin subunit beta-4
Karyopherin-123
Ran-binding protein YRB4

Gene names

Name:	KAP123
Synonyms:	YRB4
Ordered Locus Names:	YER110C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1113 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for nuclear protein import, its predominant substrate seems to be ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran).
Subcellular location	Cytoplasm. Nucleus. Nucleus › nuclear pore complex.
Miscellaneous	Present with 38300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the importin beta family. Contains 11 HEAT repeats. Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Cytoplasm Nuclear pore complex Nucleus
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW protein import into nucleus Inferred from mutant phenotype Ref.3. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nuclear pore Inferred from direct assay. Source: SGD
Molecular function	binding Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from mutant phenotype Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1113

1113

Importin subunit beta-4

PRO_0000120776

Regions

Domain

25 – 92

68

Importin N-terminal

Repeat

117 – 154

38

HEAT 1

Repeat

158 – 196

39

HEAT 2

Repeat

393 – 430

38

HEAT 3

Repeat

434 – 472

39

HEAT 4

Repeat

477 – 515

39

HEAT 5

Repeat

522 – 565

44

HEAT 6

Repeat

570 – 607

38

HEAT 7

Repeat

611 – 649

39

HEAT 8

Repeat

859 – 896

38

HEAT 9

Repeat

941 – 979

39

HEAT 10

Repeat

1016 – 1053

38

HEAT 11

Amino acid modifications

Modified residue

646

1

Phosphoserine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P40069 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 9DC524C28BB71A46
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FASTA

1,113

122,601

        10         20         30         40         50         60 
MDQQFLSQLE QTLHAITSGV GLKEATKTLQ TQFYTQPTTL PALIHILQNG SDDSLKQLAG 

        70         80         90        100        110        120 
VEARKLVSKH WNAIDESTRA SIKTSLLQTA FSEPKENVRH SNARVIASIG TEELDGNKWP 

       130        140        150        160        170        180 
DLVPNLIQTA SGEDVQTRQT AIFILFSLLE DFTSSLSGHI DDFLALFSQT INDPSSLEIR 

       190        200        210        220        230        240 
SLSAQALNHV SALIEEQETI NPVQAQKFAA SIPSVVNVLD AVIKADDTMN AKLIFNCLND 

       250        260        270        280        290        300 
FLLLDSQLTG NFIVDLIKLS LQIAVNSEID EDVRVFALQF IISSLSYRKS KVSQSKLGPE 

       310        320        330        340        350        360 
ITVAALKVAC EEIDVDDELN NEDETGENEE NTPSSSAIRL LAFASSELPP SQVASVIVEH 

       370        380        390        400        410        420 
IPAMLQSANV FERRAILLAI SVAVTGSPDY ILSQFDKIIP ATINGLKDTE PIVKLAALKC 

       430        440        450        460        470        480 
IHQLTTDLQD EVAKFHEEYL PLIIDIIDSA KNIVIYNYAT VALDGLLEFI AYDAIAKYLD 

       490        500        510        520        530        540 
PLMNKLFYML ESNESSKLRC AVVSAIGSAA FAAGSAFIPY FKTSVHYLEK FIQNCSQIEG 

       550        560        570        580        590        600 
MSEDDIELRA NTFENISTMA RAVRSDAFAE FAEPLVNSAY EAIKTDSARL RESGYAFIAN 

       610        620        630        640        650        660 
LAKVYGENFA PFLKTILPEI FKTLELDEYQ FNFDGDAEDL AAFADSANEE ELQNKFTVNT 

       670        680        690        700        710        720 
GISYEKEVAS AALSELALGT KEHFLPYVEQ SLKVLNEQVD ESYGLRETAL NTIWNVVKSV 

       730        740        750        760        770        780 
LLASKVEPES YPKGIPASSY VNADVLAVIQ AARETSMGNL SDEFETSMVI TVMEDFANMI 

       790        800        810        820        830        840 
KQFGAIIIMD NGDSSMLEAL CMQVLSVLKG THTCQTIDIE EDVPRDEELD ASETEATLQD 

       850        860        870        880        890        900 
VALEVLVSLS QALAGDFAKV FDNFRPVVFG LFQSKSKNKR SSAVGAASEL ALGMKEQNPF 

       910        920        930        940        950        960 
VHEMLEALVI RLTSDKSLEV RGNAAYGVGL LCEYASMDIS AVYEPVLKAL YELLSAADQK 

       970        980        990       1000       1010       1020 
ALAAEDDEAT REIIDRAYAN ASGCVARMAL KNSALVPLEQ TVPALLAHLP LNTGFEEYNP 

      1030       1040       1050       1060       1070       1080 
IFELIMKLYQ ENSPVITNET PRIIEIFSAV FTKENDRIKL EKESTLGREE NMERLKQFQT 

      1090       1100       1110 
EEMKHKVIEL LKYLNTTYNG IVAQNPVLAA VIA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus." Schlenstedt G., Smirnova E., Deane R., Solsbacher J., Kutay U., Goerlich D., Ponstingl H., Bischoff F.R. EMBO J. 16:6237-6249(1997) [PubMed: 9321403] [Abstract] Cited for: PROTEIN SEQUENCE OF 895-903 AND 1073-1099, CHARACTERIZATION.
[4]	"A distinct nuclear import pathway used by ribosomal proteins." Rout M.P., Blobel G., Aitchison J.D. Cell 89:715-725(1997) [PubMed: 9182759] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-646, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18916 Genomic DNA. Translation: AAC03208.1. BK006939 Genomic DNA. Translation: DAA07770.1.
PIR	S50613.
RefSeq	NP_011035.1. NM_001179000.1.
3D structure databases
ProteinModelPortal	P40069.
SMR	P40069. Positions 2-196, 358-468, 590-621, 668-714, 863-942.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2356N.
IntAct	P40069. 153 interactions.
MINT	MINT-623443.
STRING	P40069.
Proteomic databases
PeptideAtlas	P40069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER110C; YER110C; YER110C.
GeneID	856846.
KEGG	sce:YER110C.
NMPDR	fig\|4932.3.peg.2111.
Organism-specific databases
CYGD	YER110c.
SGD	S000000912. KAP123.
Phylogenomic databases
eggNOG	fuNOG04083.
GeneTree	EFGT00050000005847.
HOGENOM	HBG395949.
OMA	FLEFTND.
OrthoDB	EOG46DQ9V.
Gene expression databases
ArrayExpress	P40069.
Genevestigator	P40069.
GermOnline	YER110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR021133. HEAT_type_2. IPR001494. Importin-beta_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 3 hits.
Pfam	PF03810. IBN_N. 1 hit. [Graphical view]
SMART	SM00913. IBN_N. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. 1 hit. PS50166. IMPORTIN_B_NT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983171.

Entry information

Entry name

IMB4_YEAST

Accession

Primary (citable) accession number: P40069
Secondary accession number(s): D3DM16

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents