Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Importin subunit beta-4

Gene

KAP123

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Its predominant cargo substrate seems to be ribosomal proteins (PubMed:9321403). Required for import of the ribbosomal assembly factor NMD3 (PubMed:12612077). May be involved in nuclear transport of YAP1 (PubMed:11274141). Mediates the nuclear import of histones H3 and H4 (PubMed:11694505). Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo (PubMed:9321403). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication3 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • protein transporter activity Source: SGD

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: SGD
  • protein import into nucleus Source: SGD
  • protein import into nucleus, docking Source: GO_Central
  • protein import into nucleus, translocation Source: GO_Central
  • regulation of pseudohyphal growth Source: SGD
  • ribosomal protein import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-4Curated
Alternative name(s):
Importin-123
Karyopherin subunit beta-4Curated
Karyopherin-1231 Publication
Ran-binding protein YRB41 Publication
Gene namesi
Name:KAP1231 Publication
Synonyms:YRB41 Publication
Ordered Locus Names:YER110CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER110C.
SGDiS000000912. KAP123.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • nuclear pore Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207761 – 1113Importin subunit beta-4Add BLAST1113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei646PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40069.
PRIDEiP40069.

PTM databases

iPTMnetiP40069.

Interactioni

Subunit structurei

Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with RPL25; this interaction is dissociated by binding to Ran-GTP (PubMed:9321403). Interacts with YAP1 (PubMed:11274141). Interacts with histones H3 and H4; via their NLS (PubMed:11694505).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NBP1P529196EBI-9166,EBI-11886

Protein-protein interaction databases

BioGridi36855. 182 interactors.
DIPiDIP-2356N.
IntActiP40069. 176 interactors.
MINTiMINT-623443.

Structurei

3D structure databases

ProteinModelPortaliP40069.
SMRiP40069.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati3 – 35HEAT 1By similarity1 PublicationAdd BLAST33
Domaini25 – 92Importin N-terminalPROSITE-ProRule annotationAdd BLAST68
Repeati40 – 74HEAT 2By similarity1 PublicationAdd BLAST35
Repeati92 – 115HEAT 3By similarity1 PublicationAdd BLAST24
Repeati120 – 147HEAT 4By similarity1 PublicationAdd BLAST28
Repeati157 – 191HEAT 5By similarity1 PublicationAdd BLAST35
Repeati208 – 244HEAT 6By similarity1 PublicationAdd BLAST37
Repeati252 – 287HEAT 7By similarity1 PublicationAdd BLAST36
Repeati296 – 348HEAT 8By similarity1 PublicationAdd BLAST53
Repeati350 – 384HEAT 9By similarity1 PublicationAdd BLAST35
Repeati388 – 428HEAT 10By similarity1 PublicationAdd BLAST41
Repeati430 – 469HEAT 11By similarity1 PublicationAdd BLAST40
Repeati472 – 513HEAT 12By similarity1 PublicationAdd BLAST42
Repeati515 – 558HEAT 13By similarity1 PublicationAdd BLAST44
Repeati560 – 605HEAT 14By similarity1 PublicationAdd BLAST46
Repeati607 – 680HEAT 15By similarity1 PublicationAdd BLAST74
Repeati683 – 737HEAT 16By similarity1 PublicationAdd BLAST55
Repeati744 – 783HEAT 17By similarity1 PublicationAdd BLAST40
Repeati790 – 856HEAT 18By similarity1 PublicationAdd BLAST67
Repeati859 – 894HEAT 19By similarity1 PublicationAdd BLAST36
Repeati902 – 934HEAT 20By similarity1 PublicationAdd BLAST33
Repeati942 – 991HEAT 21By similarity1 PublicationAdd BLAST50
Repeati999 – 1030HEAT 22By similarity1 PublicationAdd BLAST32
Repeati1049 – 1084HEAT 23By similarity1 PublicationAdd BLAST36
Repeati1085 – 1110HEAT 24By similarity1 PublicationAdd BLAST26

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 24 HEAT repeats.By similarity1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075074.
HOGENOMiHOG000246583.
InParanoidiP40069.
KOiK20221.
OMAiQSPQEDH.
OrthoDBiEOG092C0EXQ.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQQFLSQLE QTLHAITSGV GLKEATKTLQ TQFYTQPTTL PALIHILQNG
60 70 80 90 100
SDDSLKQLAG VEARKLVSKH WNAIDESTRA SIKTSLLQTA FSEPKENVRH
110 120 130 140 150
SNARVIASIG TEELDGNKWP DLVPNLIQTA SGEDVQTRQT AIFILFSLLE
160 170 180 190 200
DFTSSLSGHI DDFLALFSQT INDPSSLEIR SLSAQALNHV SALIEEQETI
210 220 230 240 250
NPVQAQKFAA SIPSVVNVLD AVIKADDTMN AKLIFNCLND FLLLDSQLTG
260 270 280 290 300
NFIVDLIKLS LQIAVNSEID EDVRVFALQF IISSLSYRKS KVSQSKLGPE
310 320 330 340 350
ITVAALKVAC EEIDVDDELN NEDETGENEE NTPSSSAIRL LAFASSELPP
360 370 380 390 400
SQVASVIVEH IPAMLQSANV FERRAILLAI SVAVTGSPDY ILSQFDKIIP
410 420 430 440 450
ATINGLKDTE PIVKLAALKC IHQLTTDLQD EVAKFHEEYL PLIIDIIDSA
460 470 480 490 500
KNIVIYNYAT VALDGLLEFI AYDAIAKYLD PLMNKLFYML ESNESSKLRC
510 520 530 540 550
AVVSAIGSAA FAAGSAFIPY FKTSVHYLEK FIQNCSQIEG MSEDDIELRA
560 570 580 590 600
NTFENISTMA RAVRSDAFAE FAEPLVNSAY EAIKTDSARL RESGYAFIAN
610 620 630 640 650
LAKVYGENFA PFLKTILPEI FKTLELDEYQ FNFDGDAEDL AAFADSANEE
660 670 680 690 700
ELQNKFTVNT GISYEKEVAS AALSELALGT KEHFLPYVEQ SLKVLNEQVD
710 720 730 740 750
ESYGLRETAL NTIWNVVKSV LLASKVEPES YPKGIPASSY VNADVLAVIQ
760 770 780 790 800
AARETSMGNL SDEFETSMVI TVMEDFANMI KQFGAIIIMD NGDSSMLEAL
810 820 830 840 850
CMQVLSVLKG THTCQTIDIE EDVPRDEELD ASETEATLQD VALEVLVSLS
860 870 880 890 900
QALAGDFAKV FDNFRPVVFG LFQSKSKNKR SSAVGAASEL ALGMKEQNPF
910 920 930 940 950
VHEMLEALVI RLTSDKSLEV RGNAAYGVGL LCEYASMDIS AVYEPVLKAL
960 970 980 990 1000
YELLSAADQK ALAAEDDEAT REIIDRAYAN ASGCVARMAL KNSALVPLEQ
1010 1020 1030 1040 1050
TVPALLAHLP LNTGFEEYNP IFELIMKLYQ ENSPVITNET PRIIEIFSAV
1060 1070 1080 1090 1100
FTKENDRIKL EKESTLGREE NMERLKQFQT EEMKHKVIEL LKYLNTTYNG
1110
IVAQNPVLAA VIA
Length:1,113
Mass (Da):122,601
Last modified:February 1, 1995 - v1
Checksum:i9DC524C28BB71A46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03208.1.
BK006939 Genomic DNA. Translation: DAA07770.1.
PIRiS50613.
RefSeqiNP_011035.1. NM_001179000.1.

Genome annotation databases

EnsemblFungiiYER110C; YER110C; YER110C.
GeneIDi856846.
KEGGisce:YER110C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03208.1.
BK006939 Genomic DNA. Translation: DAA07770.1.
PIRiS50613.
RefSeqiNP_011035.1. NM_001179000.1.

3D structure databases

ProteinModelPortaliP40069.
SMRiP40069.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36855. 182 interactors.
DIPiDIP-2356N.
IntActiP40069. 176 interactors.
MINTiMINT-623443.

PTM databases

iPTMnetiP40069.

Proteomic databases

MaxQBiP40069.
PRIDEiP40069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER110C; YER110C; YER110C.
GeneIDi856846.
KEGGisce:YER110C.

Organism-specific databases

EuPathDBiFungiDB:YER110C.
SGDiS000000912. KAP123.

Phylogenomic databases

GeneTreeiENSGT00550000075074.
HOGENOMiHOG000246583.
InParanoidiP40069.
KOiK20221.
OMAiQSPQEDH.
OrthoDBiEOG092C0EXQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30274-MONOMER.

Miscellaneous databases

PROiP40069.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMB4_YEAST
AccessioniPrimary (citable) accession number: P40069
Secondary accession number(s): D3DM16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 38300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.