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Protein

Importin subunit beta-4

Gene

KAP123

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Its predominant cargo substrate seems to be ribosomal proteins (PubMed:9321403). Required for import of the ribbosomal assembly factor NMD3 (PubMed:12612077). May be involved in nuclear transport of YAP1 (PubMed:11274141). Mediates the nuclear import of histones H3 and H4 (PubMed:11694505). Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo (PubMed:9321403). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication3 Publications

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • protein transporter activity Source: SGD

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • NLS-bearing protein import into nucleus Source: SGD
  • protein import into nucleus Source: SGD
  • regulation of pseudohyphal growth Source: SGD
  • ribosomal protein import into nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30274-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-4Curated
Alternative name(s):
Importin-123
Karyopherin subunit beta-4Curated
Karyopherin-1231 Publication
Ran-binding protein YRB41 Publication
Gene namesi
Name:KAP1231 Publication
Synonyms:YRB41 Publication
Ordered Locus Names:YER110CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER110C.
SGDiS000000912. KAP123.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear pore Source: UniProtKB-SubCell
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11131113Importin subunit beta-4PRO_0000120776Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei646 – 6461PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40069.
PeptideAtlasiP40069.

PTM databases

iPTMnetiP40069.

Interactioni

Subunit structurei

Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with RPL25; this interaction is dissociated by binding to Ran-GTP (PubMed:9321403). Interacts with YAP1 (PubMed:11274141). Interacts with histones H3 and H4; via their NLS (PubMed:11694505).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NBP1P529196EBI-9166,EBI-11886

Protein-protein interaction databases

BioGridi36855. 182 interactions.
DIPiDIP-2356N.
IntActiP40069. 176 interactions.
MINTiMINT-623443.

Structurei

3D structure databases

ProteinModelPortaliP40069.
SMRiP40069. Positions 35-148, 358-426, 864-931.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati3 – 3533HEAT 1By similarity1 PublicationAdd
BLAST
Domaini25 – 9268Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati40 – 7435HEAT 2By similarity1 PublicationAdd
BLAST
Repeati92 – 11524HEAT 3By similarity1 PublicationAdd
BLAST
Repeati120 – 14728HEAT 4By similarity1 PublicationAdd
BLAST
Repeati157 – 19135HEAT 5By similarity1 PublicationAdd
BLAST
Repeati208 – 24437HEAT 6By similarity1 PublicationAdd
BLAST
Repeati252 – 28736HEAT 7By similarity1 PublicationAdd
BLAST
Repeati296 – 34853HEAT 8By similarity1 PublicationAdd
BLAST
Repeati350 – 38435HEAT 9By similarity1 PublicationAdd
BLAST
Repeati388 – 42841HEAT 10By similarity1 PublicationAdd
BLAST
Repeati430 – 46940HEAT 11By similarity1 PublicationAdd
BLAST
Repeati472 – 51342HEAT 12By similarity1 PublicationAdd
BLAST
Repeati515 – 55844HEAT 13By similarity1 PublicationAdd
BLAST
Repeati560 – 60546HEAT 14By similarity1 PublicationAdd
BLAST
Repeati607 – 68074HEAT 15By similarity1 PublicationAdd
BLAST
Repeati683 – 73755HEAT 16By similarity1 PublicationAdd
BLAST
Repeati744 – 78340HEAT 17By similarity1 PublicationAdd
BLAST
Repeati790 – 85667HEAT 18By similarity1 PublicationAdd
BLAST
Repeati859 – 89436HEAT 19By similarity1 PublicationAdd
BLAST
Repeati902 – 93433HEAT 20By similarity1 PublicationAdd
BLAST
Repeati942 – 99150HEAT 21By similarity1 PublicationAdd
BLAST
Repeati999 – 103032HEAT 22By similarity1 PublicationAdd
BLAST
Repeati1049 – 108436HEAT 23By similarity1 PublicationAdd
BLAST
Repeati1085 – 111026HEAT 24By similarity1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 24 HEAT repeats.By similarity1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075074.
HOGENOMiHOG000246583.
InParanoidiP40069.
OMAiQSPQEDH.
OrthoDBiEOG7F7WJ5.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQQFLSQLE QTLHAITSGV GLKEATKTLQ TQFYTQPTTL PALIHILQNG
60 70 80 90 100
SDDSLKQLAG VEARKLVSKH WNAIDESTRA SIKTSLLQTA FSEPKENVRH
110 120 130 140 150
SNARVIASIG TEELDGNKWP DLVPNLIQTA SGEDVQTRQT AIFILFSLLE
160 170 180 190 200
DFTSSLSGHI DDFLALFSQT INDPSSLEIR SLSAQALNHV SALIEEQETI
210 220 230 240 250
NPVQAQKFAA SIPSVVNVLD AVIKADDTMN AKLIFNCLND FLLLDSQLTG
260 270 280 290 300
NFIVDLIKLS LQIAVNSEID EDVRVFALQF IISSLSYRKS KVSQSKLGPE
310 320 330 340 350
ITVAALKVAC EEIDVDDELN NEDETGENEE NTPSSSAIRL LAFASSELPP
360 370 380 390 400
SQVASVIVEH IPAMLQSANV FERRAILLAI SVAVTGSPDY ILSQFDKIIP
410 420 430 440 450
ATINGLKDTE PIVKLAALKC IHQLTTDLQD EVAKFHEEYL PLIIDIIDSA
460 470 480 490 500
KNIVIYNYAT VALDGLLEFI AYDAIAKYLD PLMNKLFYML ESNESSKLRC
510 520 530 540 550
AVVSAIGSAA FAAGSAFIPY FKTSVHYLEK FIQNCSQIEG MSEDDIELRA
560 570 580 590 600
NTFENISTMA RAVRSDAFAE FAEPLVNSAY EAIKTDSARL RESGYAFIAN
610 620 630 640 650
LAKVYGENFA PFLKTILPEI FKTLELDEYQ FNFDGDAEDL AAFADSANEE
660 670 680 690 700
ELQNKFTVNT GISYEKEVAS AALSELALGT KEHFLPYVEQ SLKVLNEQVD
710 720 730 740 750
ESYGLRETAL NTIWNVVKSV LLASKVEPES YPKGIPASSY VNADVLAVIQ
760 770 780 790 800
AARETSMGNL SDEFETSMVI TVMEDFANMI KQFGAIIIMD NGDSSMLEAL
810 820 830 840 850
CMQVLSVLKG THTCQTIDIE EDVPRDEELD ASETEATLQD VALEVLVSLS
860 870 880 890 900
QALAGDFAKV FDNFRPVVFG LFQSKSKNKR SSAVGAASEL ALGMKEQNPF
910 920 930 940 950
VHEMLEALVI RLTSDKSLEV RGNAAYGVGL LCEYASMDIS AVYEPVLKAL
960 970 980 990 1000
YELLSAADQK ALAAEDDEAT REIIDRAYAN ASGCVARMAL KNSALVPLEQ
1010 1020 1030 1040 1050
TVPALLAHLP LNTGFEEYNP IFELIMKLYQ ENSPVITNET PRIIEIFSAV
1060 1070 1080 1090 1100
FTKENDRIKL EKESTLGREE NMERLKQFQT EEMKHKVIEL LKYLNTTYNG
1110
IVAQNPVLAA VIA
Length:1,113
Mass (Da):122,601
Last modified:February 1, 1995 - v1
Checksum:i9DC524C28BB71A46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03208.1.
BK006939 Genomic DNA. Translation: DAA07770.1.
PIRiS50613.
RefSeqiNP_011035.1. NM_001179000.1.

Genome annotation databases

EnsemblFungiiYER110C; YER110C; YER110C.
GeneIDi856846.
KEGGisce:YER110C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18916 Genomic DNA. Translation: AAC03208.1.
BK006939 Genomic DNA. Translation: DAA07770.1.
PIRiS50613.
RefSeqiNP_011035.1. NM_001179000.1.

3D structure databases

ProteinModelPortaliP40069.
SMRiP40069. Positions 35-148, 358-426, 864-931.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36855. 182 interactions.
DIPiDIP-2356N.
IntActiP40069. 176 interactions.
MINTiMINT-623443.

PTM databases

iPTMnetiP40069.

Proteomic databases

MaxQBiP40069.
PeptideAtlasiP40069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER110C; YER110C; YER110C.
GeneIDi856846.
KEGGisce:YER110C.

Organism-specific databases

EuPathDBiFungiDB:YER110C.
SGDiS000000912. KAP123.

Phylogenomic databases

GeneTreeiENSGT00550000075074.
HOGENOMiHOG000246583.
InParanoidiP40069.
OMAiQSPQEDH.
OrthoDBiEOG7F7WJ5.

Enzyme and pathway databases

BioCyciYEAST:G3O-30274-MONOMER.

Miscellaneous databases

PROiP40069.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins."
    Aitchison J.D., Blobel G., Rout M.P.
    Science 274:624-627(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  4. "Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus."
    Schlenstedt G., Smirnova E., Deane R., Solsbacher J., Kutay U., Goerlich D., Ponstingl H., Bischoff F.R.
    EMBO J. 16:6237-6249(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 895-903 AND 1073-1099, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GSP1 AND RPL25.
  5. "A distinct nuclear import pathway used by ribosomal proteins."
    Rout M.P., Blobel G., Aitchison J.D.
    Cell 89:715-725(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Importin/karyopherin protein family members required for mRNA export from the nucleus."
    Seedorf M., Silver P.A.
    Proc. Natl. Acad. Sci. U.S.A. 94:8590-8595(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Importin-beta-like nuclear transport receptors."
    Stroem A.C., Weis K.
    Genome Biol. 2:REVIEWS3008.1-REVIEWS3008.9(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  8. "Nuclear import of the yeast AP-1-like transcription factor Yap1p is mediated by transport receptor Pse1p, and this import step is not affected by oxidative stress."
    Isoyama T., Murayama A., Nomoto A., Kuge S.
    J. Biol. Chem. 276:21863-21869(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YAP1.
  9. "Pathways mediating the nuclear import of histones H3 and H4 in yeast."
    Mosammaparast N., Guo Y., Shabanowitz J., Hunt D.F., Pemberton L.F.
    J. Biol. Chem. 277:862-868(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H3 AND H4.
  10. "Intersection of the Kap123p-mediated nuclear import and ribosome export pathways."
    Sydorskyy Y., Dilworth D.J., Yi E.C., Goodlett D.R., Wozniak R.W., Aitchison J.D.
    Mol. Cell. Biol. 23:2042-2054(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-646, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Structural basis for cell-cycle-dependent nuclear import mediated by the karyopherin Kap121p."
    Kobayashi J., Matsuura Y.
    J. Mol. Biol. 425:1852-1868(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEAT STRUCTURE.

Entry informationi

Entry nameiIMB4_YEAST
AccessioniPrimary (citable) accession number: P40069
Secondary accession number(s): D3DM16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 38300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.