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P40068

- FLO8_YEAST

UniProt

P40068 - FLO8_YEAST

Protein

Transcriptional activator FLO8

Gene

FLO8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Required for diploid filamentous growth, haploid invasive growth and flocculation. Putative transcriptional activator of FLO1.2 Publications

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD

    GO - Biological processi

    1. biofilm formation Source: SGD
    2. positive regulation of flocculation via cell wall protein-carbohydrate interaction by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    5. positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30273-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator FLO8
    Alternative name(s):
    Protein PDH5
    Gene namesi
    Name:FLO8
    Synonyms:PDH5
    Ordered Locus Names:YER109C
    ORF Names:YER108C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000911. FLO8.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 799799Transcriptional activator FLO8PRO_0000087303Add
    BLAST

    Proteomic databases

    PRIDEiP40068.

    Expressioni

    Gene expression databases

    GenevestigatoriP40068.

    Interactioni

    Protein-protein interaction databases

    BioGridi36854. 48 interactions.
    DIPiDIP-5470N.
    IntActiP40068. 2 interactions.
    MINTiMINT-527994.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 10533LisHPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 5515Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the FLO8 family.Curated
    Contains 1 LisH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG12793.
    OrthoDBiEOG72VHJW.

    Family and domain databases

    InterProiIPR006594. LisH_dimerisation.
    IPR013720. LisH_dimerisation_subgr.
    [Graphical view]
    PfamiPF08513. LisH. 1 hit.
    [Graphical view]
    SMARTiSM00667. LisH. 1 hit.
    [Graphical view]
    PROSITEiPS50896. LISH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40068-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYKVNSSYP DSIPPTEQPY MASQYKQDLQ SNIAMATNSE QQRQQQQQQQ    50
    QQQQQWINQP TAENSDLKEK MNCKNTLNEY IFDFLTKSSL KNTAAAFAQD 100
    AHLDRDKGQN PVDGPKSKEN NGNQNTFSKV VDTPQGFLYE WWQIFWDIFN 150
    TSSSRGGSEF AQQYYQLVLQ EQRQEQIYRS LAVHAARLQH DAERRGEYSN 200
    EDIDPMHLAA MMLGNPMAPA VQMRNVNMNP IPIPMVGNPI VNNFSIPPYN 250
    NANPTTGATA VAPTAPPSGD FTNVGPTQNR SQNVTGWPVY NYPMQPTTEN 300
    PVGNPCNNNT TNNTTNNKSP VNQPKSLKTM HSTDKPNNVP TSKSTRSRSA 350
    TSKAKGKVKA GLVAKRRRKN NTATVSAGST NACSPNITTP GSTTSEPAMV 400
    GSRVNKTPRS DIATNFRNQA IIFGEEDIYS NSKSSPSLDG ASPSALASKQ 450
    PTKVRKNTKK ASTSAFPVES TNKLGGNSVV TGKKRSPPNT RVSRRKSTPS 500
    VILNADATKD ENNMLRTFSN TIAPNIHSAP PTKTANSLPF PGINLGSFNK 550
    PAVSSPLSSV TESCFDPESG KIAGKNGPKR AVNSKVSASS PLSIATPRSG 600
    DAQKQRSSKV PGNVVIKPPH GFSTTNLNIT LKNSKIITSQ NNTVSQELPN 650
    GGNILEAQVG NDSRSSKGNR NTLSTPEEKK PSSNNQGYDF DALKNSSSLL 700
    FPNQAYASNN RTPNENSNVA DETSASTNSG DNDNTLIQPS SNVGTTLGPQ 750
    QTSTNENQNV HSQNLKFGNI GMVEDQGPDY DLNLLDTNEN DFNFINWEG 799
    Length:799
    Mass (Da):86,835
    Last modified:November 1, 1997 - v2
    Checksum:i91AAE10D8E586DAF
    GO

    Sequence cautioni

    The sequence BAA12076.1 differs from that shown. Reason: Frameshift at position 62.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti54 – 541Q → QQQ in strain: Diastaticus / ATCC 60715 / YIY2-11A.
    Natural varianti112 – 1121V → I in strain: Diastaticus / ATCC 60715 / YIY2-11A.
    Natural varianti115 – 1151P → S in strain: Diastaticus / ATCC 60715 / YIY2-11A.
    Natural varianti383 – 3831C → G in strain: Diastaticus / ATCC 60715 / YIY2-11A.
    Natural varianti441 – 4411A → T in strain: Diastaticus / ATCC 60715 / YIY2-11A.
    Natural varianti447 – 4471A → V in strain: Diastaticus / ATCC 60715 / YIY2-11A.
    Natural varianti598 – 5981R → P in strain: Diastaticus / ATCC 60715 / YIY2-11A.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51431 Genomic DNA. Translation: AAC49522.1.
    D83713 Genomic DNA. Translation: BAA12076.1. Frameshift.
    U18916 Genomic DNA. Translation: AAC03207.1. Sequence problems.
    U18916 Genomic DNA. Translation: AAC03206.1. Sequence problems.
    BK006939 Genomic DNA. Translation: DAA07769.1.
    PIRiS50611.
    S50612.
    RefSeqiNP_011034.3. NM_001178999.3.

    Genome annotation databases

    EnsemblFungiiYER109C; YER109C; YER109C.
    GeneIDi856845.
    KEGGisce:YER109C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51431 Genomic DNA. Translation: AAC49522.1 .
    D83713 Genomic DNA. Translation: BAA12076.1 . Frameshift.
    U18916 Genomic DNA. Translation: AAC03207.1 . Sequence problems.
    U18916 Genomic DNA. Translation: AAC03206.1 . Sequence problems.
    BK006939 Genomic DNA. Translation: DAA07769.1 .
    PIRi S50611.
    S50612.
    RefSeqi NP_011034.3. NM_001178999.3.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36854. 48 interactions.
    DIPi DIP-5470N.
    IntActi P40068. 2 interactions.
    MINTi MINT-527994.

    Proteomic databases

    PRIDEi P40068.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER109C ; YER109C ; YER109C .
    GeneIDi 856845.
    KEGGi sce:YER109C.

    Organism-specific databases

    SGDi S000000911. FLO8.

    Phylogenomic databases

    eggNOGi NOG12793.
    OrthoDBi EOG72VHJW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30273-MONOMER.

    Miscellaneous databases

    NextBioi 983168.

    Gene expression databases

    Genevestigatori P40068.

    Family and domain databases

    InterProi IPR006594. LisH_dimerisation.
    IPR013720. LisH_dimerisation_subgr.
    [Graphical view ]
    Pfami PF08513. LisH. 1 hit.
    [Graphical view ]
    SMARTi SM00667. LisH. 1 hit.
    [Graphical view ]
    PROSITEi PS50896. LISH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth."
      Liu H., Styles C.A., Fink G.R.
      Genetics 144:967-978(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: Sigma 1278B.
    2. "Molecular cloning and analysis of the dominant flocculation gene FLO8 from Saccharomyces cerevisiae."
      Kobayashi O., Suda H., Ohtani T., Sone H.
      Mol. Gen. Genet. 251:707-715(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: Diastaticus / ATCC 60715 / YIY2-11A.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiFLO8_YEAST
    AccessioniPrimary (citable) accession number: P40068
    Secondary accession number(s): D3DM15, P40067, Q05751
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Strain S288c has a stop codon in position 142, which disrupts the gene coding for this protein and produces two ORFs.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3