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Protein

Transcriptional activator FLO8

Gene

FLO8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Required for diploid filamentous growth, haploid invasive growth and flocculation. Putative transcriptional activator of FLO1.2 Publications

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD

GO - Biological processi

  1. positive regulation of cell adhesion involved in single-species biofilm formation Source: SGD
  2. positive regulation of flocculation via cell wall protein-carbohydrate interaction by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  3. positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  4. positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter Source: SGD
  5. positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator FLO8
Alternative name(s):
Protein PDH5
Gene namesi
Name:FLO8
Synonyms:PDH5
Ordered Locus Names:YER109C
ORF Names:YER108C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000911. FLO8.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 799799Transcriptional activator FLO8PRO_0000087303Add
BLAST

Proteomic databases

PRIDEiP40068.

Expressioni

Gene expression databases

GenevestigatoriP40068.

Interactioni

Protein-protein interaction databases

BioGridi36854. 48 interactions.
DIPiDIP-5470N.
IntActiP40068. 2 interactions.
MINTiMINT-527994.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 10533LisHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 5515Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the FLO8 family.Curated
Contains 1 LisH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG12793.
InParanoidiP40068.
OrthoDBiEOG72VHJW.

Family and domain databases

InterProiIPR006594. LisH_dimerisation.
IPR013720. LisH_dimerisation_subgr.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKVNSSYP DSIPPTEQPY MASQYKQDLQ SNIAMATNSE QQRQQQQQQQ
60 70 80 90 100
QQQQQWINQP TAENSDLKEK MNCKNTLNEY IFDFLTKSSL KNTAAAFAQD
110 120 130 140 150
AHLDRDKGQN PVDGPKSKEN NGNQNTFSKV VDTPQGFLYE WWQIFWDIFN
160 170 180 190 200
TSSSRGGSEF AQQYYQLVLQ EQRQEQIYRS LAVHAARLQH DAERRGEYSN
210 220 230 240 250
EDIDPMHLAA MMLGNPMAPA VQMRNVNMNP IPIPMVGNPI VNNFSIPPYN
260 270 280 290 300
NANPTTGATA VAPTAPPSGD FTNVGPTQNR SQNVTGWPVY NYPMQPTTEN
310 320 330 340 350
PVGNPCNNNT TNNTTNNKSP VNQPKSLKTM HSTDKPNNVP TSKSTRSRSA
360 370 380 390 400
TSKAKGKVKA GLVAKRRRKN NTATVSAGST NACSPNITTP GSTTSEPAMV
410 420 430 440 450
GSRVNKTPRS DIATNFRNQA IIFGEEDIYS NSKSSPSLDG ASPSALASKQ
460 470 480 490 500
PTKVRKNTKK ASTSAFPVES TNKLGGNSVV TGKKRSPPNT RVSRRKSTPS
510 520 530 540 550
VILNADATKD ENNMLRTFSN TIAPNIHSAP PTKTANSLPF PGINLGSFNK
560 570 580 590 600
PAVSSPLSSV TESCFDPESG KIAGKNGPKR AVNSKVSASS PLSIATPRSG
610 620 630 640 650
DAQKQRSSKV PGNVVIKPPH GFSTTNLNIT LKNSKIITSQ NNTVSQELPN
660 670 680 690 700
GGNILEAQVG NDSRSSKGNR NTLSTPEEKK PSSNNQGYDF DALKNSSSLL
710 720 730 740 750
FPNQAYASNN RTPNENSNVA DETSASTNSG DNDNTLIQPS SNVGTTLGPQ
760 770 780 790
QTSTNENQNV HSQNLKFGNI GMVEDQGPDY DLNLLDTNEN DFNFINWEG
Length:799
Mass (Da):86,835
Last modified:November 1, 1997 - v2
Checksum:i91AAE10D8E586DAF
GO

Sequence cautioni

The sequence BAA12076.1 differs from that shown. Reason: Frameshift at position 62. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti54 – 541Q → QQQ in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural varianti112 – 1121V → I in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural varianti115 – 1151P → S in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural varianti383 – 3831C → G in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural varianti441 – 4411A → T in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural varianti447 – 4471A → V in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural varianti598 – 5981R → P in strain: Diastaticus / ATCC 60715 / YIY2-11A.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51431 Genomic DNA. Translation: AAC49522.1.
D83713 Genomic DNA. Translation: BAA12076.1. Frameshift.
U18916 Genomic DNA. Translation: AAC03207.1. Sequence problems.
U18916 Genomic DNA. Translation: AAC03206.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07769.1.
PIRiS50611.
S50612.
RefSeqiNP_011034.3. NM_001178999.3.

Genome annotation databases

EnsemblFungiiYER109C; YER109C; YER109C.
GeneIDi856845.
KEGGisce:YER109C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51431 Genomic DNA. Translation: AAC49522.1.
D83713 Genomic DNA. Translation: BAA12076.1. Frameshift.
U18916 Genomic DNA. Translation: AAC03207.1. Sequence problems.
U18916 Genomic DNA. Translation: AAC03206.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07769.1.
PIRiS50611.
S50612.
RefSeqiNP_011034.3. NM_001178999.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36854. 48 interactions.
DIPiDIP-5470N.
IntActiP40068. 2 interactions.
MINTiMINT-527994.

Proteomic databases

PRIDEiP40068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER109C; YER109C; YER109C.
GeneIDi856845.
KEGGisce:YER109C.

Organism-specific databases

SGDiS000000911. FLO8.

Phylogenomic databases

eggNOGiNOG12793.
InParanoidiP40068.
OrthoDBiEOG72VHJW.

Enzyme and pathway databases

BioCyciYEAST:G3O-30273-MONOMER.

Miscellaneous databases

NextBioi983168.

Gene expression databases

GenevestigatoriP40068.

Family and domain databases

InterProiIPR006594. LisH_dimerisation.
IPR013720. LisH_dimerisation_subgr.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
[Graphical view]
SMARTiSM00667. LisH. 1 hit.
[Graphical view]
PROSITEiPS50896. LISH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth."
    Liu H., Styles C.A., Fink G.R.
    Genetics 144:967-978(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: Sigma 1278B.
  2. "Molecular cloning and analysis of the dominant flocculation gene FLO8 from Saccharomyces cerevisiae."
    Kobayashi O., Suda H., Ohtani T., Sone H.
    Mol. Gen. Genet. 251:707-715(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: Diastaticus / ATCC 60715 / YIY2-11A.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiFLO8_YEAST
AccessioniPrimary (citable) accession number: P40068
Secondary accession number(s): D3DM15, P40067, Q05751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Strain S288c has a stop codon in position 142, which disrupts the gene coding for this protein and produces two ORFs.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.