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P40068 (FLO8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional activator FLO8
Alternative name(s):
Protein PDH5
Gene names
Name:FLO8
Synonyms:PDH5
Ordered Locus Names:YER109C
ORF Names:YER108C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length799 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for diploid filamentous growth, haploid invasive growth and flocculation. Putative transcriptional activator of FLO1. Ref.1 Ref.2

Subcellular location

Nucleus.

Miscellaneous

Strain S288c has a stop codon in position 142, which disrupts the gene coding for this protein and produces two ORFs.

Sequence similarities

Belongs to the FLO8 family.

Contains 1 LisH domain.

Sequence caution

The sequence BAA12076.1 differs from that shown. Reason: Frameshift at position 62.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbiofilm formation

Inferred from mutant phenotype PubMed 11157168PubMed 18202364. Source: SGD

positive regulation of flocculation via cell wall protein-carbohydrate interaction by positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 10591965PubMed 16568252. Source: SGD

positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 10373537PubMed 10591965PubMed 16568252. Source: SGD

positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12024012. Source: SGD

positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 10591965. Source: SGD

   Cellular_componentnucleus

Inferred from direct assay PubMed 12024012Ref.1. Source: SGD

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 12024012. Source: SGD

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 10515942. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 799799Transcriptional activator FLO8
PRO_0000087303

Regions

Domain73 – 10533LisH
Compositional bias41 – 5515Poly-Gln

Natural variations

Natural variant541Q → QQQ in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural variant1121V → I in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural variant1151P → S in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural variant3831C → G in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural variant4411A → T in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural variant4471A → V in strain: Diastaticus / ATCC 60715 / YIY2-11A.
Natural variant5981R → P in strain: Diastaticus / ATCC 60715 / YIY2-11A.

Sequences

Sequence LengthMass (Da)Tools
P40068 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 91AAE10D8E586DAF

FASTA79986,835
        10         20         30         40         50         60 
MSYKVNSSYP DSIPPTEQPY MASQYKQDLQ SNIAMATNSE QQRQQQQQQQ QQQQQWINQP 

        70         80         90        100        110        120 
TAENSDLKEK MNCKNTLNEY IFDFLTKSSL KNTAAAFAQD AHLDRDKGQN PVDGPKSKEN 

       130        140        150        160        170        180 
NGNQNTFSKV VDTPQGFLYE WWQIFWDIFN TSSSRGGSEF AQQYYQLVLQ EQRQEQIYRS 

       190        200        210        220        230        240 
LAVHAARLQH DAERRGEYSN EDIDPMHLAA MMLGNPMAPA VQMRNVNMNP IPIPMVGNPI 

       250        260        270        280        290        300 
VNNFSIPPYN NANPTTGATA VAPTAPPSGD FTNVGPTQNR SQNVTGWPVY NYPMQPTTEN 

       310        320        330        340        350        360 
PVGNPCNNNT TNNTTNNKSP VNQPKSLKTM HSTDKPNNVP TSKSTRSRSA TSKAKGKVKA 

       370        380        390        400        410        420 
GLVAKRRRKN NTATVSAGST NACSPNITTP GSTTSEPAMV GSRVNKTPRS DIATNFRNQA 

       430        440        450        460        470        480 
IIFGEEDIYS NSKSSPSLDG ASPSALASKQ PTKVRKNTKK ASTSAFPVES TNKLGGNSVV 

       490        500        510        520        530        540 
TGKKRSPPNT RVSRRKSTPS VILNADATKD ENNMLRTFSN TIAPNIHSAP PTKTANSLPF 

       550        560        570        580        590        600 
PGINLGSFNK PAVSSPLSSV TESCFDPESG KIAGKNGPKR AVNSKVSASS PLSIATPRSG 

       610        620        630        640        650        660 
DAQKQRSSKV PGNVVIKPPH GFSTTNLNIT LKNSKIITSQ NNTVSQELPN GGNILEAQVG 

       670        680        690        700        710        720 
NDSRSSKGNR NTLSTPEEKK PSSNNQGYDF DALKNSSSLL FPNQAYASNN RTPNENSNVA 

       730        740        750        760        770        780 
DETSASTNSG DNDNTLIQPS SNVGTTLGPQ QTSTNENQNV HSQNLKFGNI GMVEDQGPDY 

       790 
DLNLLDTNEN DFNFINWEG 

« Hide

References

« Hide 'large scale' references
[1]"Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth."
Liu H., Styles C.A., Fink G.R.
Genetics 144:967-978(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: Sigma 1278B.
[2]"Molecular cloning and analysis of the dominant flocculation gene FLO8 from Saccharomyces cerevisiae."
Kobayashi O., Suda H., Ohtani T., Sone H.
Mol. Gen. Genet. 251:707-715(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: Diastaticus / ATCC 60715 / YIY2-11A.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51431 Genomic DNA. Translation: AAC49522.1.
D83713 Genomic DNA. Translation: BAA12076.1. Frameshift.
U18916 Genomic DNA. Translation: AAC03207.1. Sequence problems.
U18916 Genomic DNA. Translation: AAC03206.1. Sequence problems.
BK006939 Genomic DNA. Translation: DAA07769.1.
PIRS50611.
S50612.
RefSeqNP_011034.3. NM_001178999.3.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36854. 48 interactions.
DIPDIP-5470N.
IntActP40068. 2 interactions.
MINTMINT-527994.

Proteomic databases

PRIDEP40068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER109C; YER109C; YER109C.
GeneID856845.
KEGGsce:YER109C.

Organism-specific databases

SGDS000000911. FLO8.

Phylogenomic databases

eggNOGNOG12793.
OrthoDBEOG72VHJW.

Enzyme and pathway databases

BioCycYEAST:G3O-30273-MONOMER.

Gene expression databases

GenevestigatorP40068.

Family and domain databases

InterProIPR006594. LisH_dimerisation.
IPR013720. LisH_dimerisation_subgr.
[Graphical view]
PfamPF08513. LisH. 1 hit.
[Graphical view]
SMARTSM00667. LisH. 1 hit.
[Graphical view]
PROSITEPS50896. LISH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983168.

Entry information

Entry nameFLO8_YEAST
AccessionPrimary (citable) accession number: P40068
Secondary accession number(s): D3DM15, P40067, Q05751
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families