P40068 (FLO8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator FLO8

Alternative name(s):
Protein PDH5

Gene names

Name:	FLO8
Synonyms:	PDH5
Ordered Locus Names:	YER109C
ORF Names:	YER108C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for diploid filamentous growth, haploid invasive growth and flocculation. Putative transcriptional activator of FLO1. Ref.1 Ref.2
Subcellular location	Nucleus.
Miscellaneous	Strain S288c has a stop codon in position 142, which disrupts the gene coding for this protein and produces two ORFs.
Sequence similarities	Belongs to the FLO8 family. Contains 1 LisH domain.
Sequence caution	The sequence BAA12076.1 differs from that shown. Reason: Frameshift at position 62.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biofilm formation Inferred from mutant phenotype PubMed 11157168PubMed 18202364. Source: SGD positive regulation of flocculation via cell wall protein-carbohydrate interaction by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 10591965PubMed 16568252. Source: SGD positive regulation of invasive growth in response to glucose limitation by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 10373537PubMed 10591965PubMed 16568252. Source: SGD positive regulation of pseudohyphal growth by positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 12024012. Source: SGD positive regulation of starch catabolic process by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 10591965. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD nucleus Inferred from direct assay PubMed 11914276PubMed 12024012Ref.1. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 12024012. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay PubMed 10515942. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 799	799	Transcriptional activator FLO8		PRO_0000087303
Regions
	Domain	73 – 105	33	LisH
	Compositional bias	41 – 55	15	Poly-Gln
Natural variations
	Natural variant	54	1	Q → QQQ in strain: Diastaticus / ATCC 60715 / YIY2-11A.
	Natural variant	112	1	V → I in strain: Diastaticus / ATCC 60715 / YIY2-11A.
	Natural variant	115	1	P → S in strain: Diastaticus / ATCC 60715 / YIY2-11A.
	Natural variant	383	1	C → G in strain: Diastaticus / ATCC 60715 / YIY2-11A.
	Natural variant	441	1	A → T in strain: Diastaticus / ATCC 60715 / YIY2-11A.
	Natural variant	447	1	A → V in strain: Diastaticus / ATCC 60715 / YIY2-11A.
	Natural variant	598	1	R → P in strain: Diastaticus / ATCC 60715 / YIY2-11A.

Sequences

	Sequence		Length	Mass (Da)
	P40068 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 91AAE10D8E586DAF	FASTA	799	86,835
	10 20 30 40 50 60 MSYKVNSSYP DSIPPTEQPY MASQYKQDLQ SNIAMATNSE QQRQQQQQQQ QQQQQWINQP 70 80 90 100 110 120 TAENSDLKEK MNCKNTLNEY IFDFLTKSSL KNTAAAFAQD AHLDRDKGQN PVDGPKSKEN 130 140 150 160 170 180 NGNQNTFSKV VDTPQGFLYE WWQIFWDIFN TSSSRGGSEF AQQYYQLVLQ EQRQEQIYRS 190 200 210 220 230 240 LAVHAARLQH DAERRGEYSN EDIDPMHLAA MMLGNPMAPA VQMRNVNMNP IPIPMVGNPI 250 260 270 280 290 300 VNNFSIPPYN NANPTTGATA VAPTAPPSGD FTNVGPTQNR SQNVTGWPVY NYPMQPTTEN 310 320 330 340 350 360 PVGNPCNNNT TNNTTNNKSP VNQPKSLKTM HSTDKPNNVP TSKSTRSRSA TSKAKGKVKA 370 380 390 400 410 420 GLVAKRRRKN NTATVSAGST NACSPNITTP GSTTSEPAMV GSRVNKTPRS DIATNFRNQA 430 440 450 460 470 480 IIFGEEDIYS NSKSSPSLDG ASPSALASKQ PTKVRKNTKK ASTSAFPVES TNKLGGNSVV 490 500 510 520 530 540 TGKKRSPPNT RVSRRKSTPS VILNADATKD ENNMLRTFSN TIAPNIHSAP PTKTANSLPF 550 560 570 580 590 600 PGINLGSFNK PAVSSPLSSV TESCFDPESG KIAGKNGPKR AVNSKVSASS PLSIATPRSG 610 620 630 640 650 660 DAQKQRSSKV PGNVVIKPPH GFSTTNLNIT LKNSKIITSQ NNTVSQELPN GGNILEAQVG 670 680 690 700 710 720 NDSRSSKGNR NTLSTPEEKK PSSNNQGYDF DALKNSSSLL FPNQAYASNN RTPNENSNVA 730 740 750 760 770 780 DETSASTNSG DNDNTLIQPS SNVGTTLGPQ QTSTNENQNV HSQNLKFGNI GMVEDQGPDY 790 DLNLLDTNEN DFNFINWEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth." Liu H., Styles C.A., Fink G.R. Genetics 144:967-978(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: Sigma 1278B.
[2]	"Molecular cloning and analysis of the dominant flocculation gene FLO8 from Saccharomyces cerevisiae." Kobayashi O., Suda H., Ohtani T., Sone H. Mol. Gen. Genet. 251:707-715(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: Diastaticus / ATCC 60715 / YIY2-11A.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51431 Genomic DNA. Translation: AAC49522.1. D83713 Genomic DNA. Translation: BAA12076.1. Frameshift. U18916 Genomic DNA. Translation: AAC03207.1. Sequence problems. U18916 Genomic DNA. Translation: AAC03206.1. Sequence problems. BK006939 Genomic DNA. Translation: DAA07769.1.
PIR	S50611. S50612.
RefSeq	NP_011034.3. NM_001178999.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5470N.
IntAct	P40068. 1 interaction.
MINT	MINT-527994.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER109C; YER109C; YER109C.
GeneID	856845.
KEGG	sce:YER109C. sce:YER113C.
Organism-specific databases
SGD	S000000911. FLO8.
Phylogenomic databases
eggNOG	NOG12793.
OrthoDB	EOG4BK8DR.
Gene expression databases
Genevestigator	P40068.
GermOnline	YER109C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006594. LisH_dimerisation. IPR013720. LisH_dimerisation_subgr. [Graphical view]
Pfam	PF08513. LisH. 1 hit. [Graphical view]
SMART	SM00667. LisH. 1 hit. [Graphical view]
PROSITE	PS50896. LISH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983168.

Entry information

Entry name

FLO8_YEAST

Accession

Primary (citable) accession number: P40068
Secondary accession number(s): D3DM15, P40067, Q05751

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program