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Protein

Nucleoporin GLE2

Gene

GLE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically important for nuclear mRNA export.4 Publications

GO - Molecular functioni

GO - Biological processi

  • nuclear pore distribution Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein import into nucleus Source: GO_Central
  • regulation of mRNA export from nucleus in response to heat stress Source: SGD
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30272-MONOMER.
ReactomeiR-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin GLE2
Alternative name(s):
Nuclear pore protein GLE2
poly(A) RNA export protein RAE1
Gene namesi
Name:GLE2
Synonyms:RAE1
Ordered Locus Names:YER107C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER107C.
SGDiS000000909. GLE2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510071 – 365Nucleoporin GLE2Add BLAST365

Proteomic databases

MaxQBiP40066.
PRIDEiP40066.

PTM databases

iPTMnetiP40066.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. GLE2 interacts with the NUP82 subcomplex (NSP1, NUP82, NUP159) via NUP116. It also interacts with NUP42.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP116Q026306EBI-22648,EBI-11703

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36853. 83 interactors.
DIPiDIP-2452N.
IntActiP40066. 17 interactors.
MINTiMINT-483363.

Structurei

3D structure databases

ProteinModelPortaliP40066.
SMRiP40066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati34 – 74WD 1Add BLAST41
Repeati78 – 117WD 2Add BLAST40
Repeati119 – 162WD 3Add BLAST44
Repeati267 – 306WD 4Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat rae1 family.Curated
Contains 4 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00530000063440.
HOGENOMiHOG000208823.
InParanoidiP40066.
KOiK14298.
OMAiCIVTGSW.
OrthoDBiEOG092C4UMO.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFNRSNTT SALGTSTAMA NEKDLANDIV INSPAEDSIS DIAFSPQQDF
60 70 80 90 100
MFSASSWDGK VRIWDVQNGV PQGRAQHESS SPVLCTRWSN DGTKVASGGC
110 120 130 140 150
DNALKLYDIA SGQTQQIGMH SAPIKVLRFV QCGPSNTECI VTGSWDKTIK
160 170 180 190 200
YWDMRQPQPV STVMMPERVY SMDNKQSLLV VATAERHIAI INLANPTTIF
210 220 230 240 250
KATTSPLKWQ TRCVACYNEA DGYAIGSVEG RCSIRYIDDG MQKKSGFSFK
260 270 280 290 300
CHRQTNPNRA PGSNGQSLVY PVNSIAFHPL YGTFVTAGGD GTFNFWDKNQ
310 320 330 340 350
RHRLKGYPTL QASIPVCSFN RNGSVFAYAL SYDWHQGHMG NRPDYPNVIR
360
LHATTDEEVK EKKKR
Length:365
Mass (Da):40,523
Last modified:February 1, 1995 - v1
Checksum:i3F5B201EB1BAEEC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64662.1.
AY692884 Genomic DNA. Translation: AAT92903.1.
BK006939 Genomic DNA. Translation: DAA07768.1.
PIRiS50610.
RefSeqiNP_011033.3. NM_001178998.3.

Genome annotation databases

EnsemblFungiiYER107C; YER107C; YER107C.
GeneIDi856844.
KEGGisce:YER107C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64662.1.
AY692884 Genomic DNA. Translation: AAT92903.1.
BK006939 Genomic DNA. Translation: DAA07768.1.
PIRiS50610.
RefSeqiNP_011033.3. NM_001178998.3.

3D structure databases

ProteinModelPortaliP40066.
SMRiP40066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36853. 83 interactors.
DIPiDIP-2452N.
IntActiP40066. 17 interactors.
MINTiMINT-483363.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP40066.

Proteomic databases

MaxQBiP40066.
PRIDEiP40066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER107C; YER107C; YER107C.
GeneIDi856844.
KEGGisce:YER107C.

Organism-specific databases

EuPathDBiFungiDB:YER107C.
SGDiS000000909. GLE2.

Phylogenomic databases

GeneTreeiENSGT00530000063440.
HOGENOMiHOG000208823.
InParanoidiP40066.
KOiK14298.
OMAiCIVTGSW.
OrthoDBiEOG092C4UMO.

Enzyme and pathway databases

BioCyciYEAST:G3O-30272-MONOMER.
ReactomeiR-SCE-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.

Miscellaneous databases

PROiP40066.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLE2_YEAST
AccessioniPrimary (citable) accession number: P40066
Secondary accession number(s): D3DM14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.