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Protein

Nucleoporin GLE2

Gene

GLE2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically important for nuclear mRNA export.4 Publications

Miscellaneous

Present with 1820 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • nuclear pore distribution Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein import into nucleus Source: GO_Central
  • regulation of mRNA export from nucleus in response to heat stress Source: SGD
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30272-MONOMER
ReactomeiR-SCE-141430 Inactivation of APC/C via direct inhibition of the APC/C complex

Protein family/group databases

TCDBi1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin GLE2
Alternative name(s):
Nuclear pore protein GLE2
poly(A) RNA export protein RAE1
Gene namesi
Name:GLE2
Synonyms:RAE1
Ordered Locus Names:YER107C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER107C
SGDiS000000909 GLE2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510071 – 365Nucleoporin GLE2Add BLAST365

Proteomic databases

MaxQBiP40066
PaxDbiP40066
PRIDEiP40066

PTM databases

iPTMnetiP40066

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEC13 and SEH1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. GLE2 interacts with the NUP82 subcomplex (NSP1, NUP82, NUP159) via NUP116. It also interacts with NUP42.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP116Q026305EBI-22648,EBI-11703

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36853, 217 interactors
DIPiDIP-2452N
IntActiP40066, 16 interactors
MINTiP40066
STRINGi4932.YER107C

Structurei

3D structure databases

ProteinModelPortaliP40066
SMRiP40066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati34 – 74WD 1Add BLAST41
Repeati78 – 117WD 2Add BLAST40
Repeati119 – 162WD 3Add BLAST44
Repeati267 – 306WD 4Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat rae1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00530000063440
HOGENOMiHOG000208823
InParanoidiP40066
KOiK14298
OMAiTIACTAF
OrthoDBiEOG092C4UMO

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR037631 Gle2/RAE1
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR10971:SF11 PTHR10971:SF11, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 4 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P40066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFNRSNTT SALGTSTAMA NEKDLANDIV INSPAEDSIS DIAFSPQQDF
60 70 80 90 100
MFSASSWDGK VRIWDVQNGV PQGRAQHESS SPVLCTRWSN DGTKVASGGC
110 120 130 140 150
DNALKLYDIA SGQTQQIGMH SAPIKVLRFV QCGPSNTECI VTGSWDKTIK
160 170 180 190 200
YWDMRQPQPV STVMMPERVY SMDNKQSLLV VATAERHIAI INLANPTTIF
210 220 230 240 250
KATTSPLKWQ TRCVACYNEA DGYAIGSVEG RCSIRYIDDG MQKKSGFSFK
260 270 280 290 300
CHRQTNPNRA PGSNGQSLVY PVNSIAFHPL YGTFVTAGGD GTFNFWDKNQ
310 320 330 340 350
RHRLKGYPTL QASIPVCSFN RNGSVFAYAL SYDWHQGHMG NRPDYPNVIR
360
LHATTDEEVK EKKKR
Length:365
Mass (Da):40,523
Last modified:February 1, 1995 - v1
Checksum:i3F5B201EB1BAEEC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA Translation: AAB64662.1
AY692884 Genomic DNA Translation: AAT92903.1
BK006939 Genomic DNA Translation: DAA07768.1
PIRiS50610
RefSeqiNP_011033.3, NM_001178998.3

Genome annotation databases

EnsemblFungiiYER107C; YER107C; YER107C
GeneIDi856844
KEGGisce:YER107C

Similar proteinsi

Entry informationi

Entry nameiGLE2_YEAST
AccessioniPrimary (citable) accession number: P40066
Secondary accession number(s): D3DM14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health