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P40065

- MAM1_YEAST

UniProt

P40065 - MAM1_YEAST

Protein

Monopolin complex subunit MAM1

Gene

MAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis.8 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. meiotic chromosome segregation Source: SGD
    2. meiotic sister chromatid cohesion involved in meiosis I Source: SGD

    Keywords - Biological processi

    Meiosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30271-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monopolin complex subunit MAM1
    Alternative name(s):
    Monopolar microtubule attachment during meiosis 1 protein 1
    Gene namesi
    Name:MAM1
    Ordered Locus Names:YER106W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER106w.
    SGDiS000000908. MAM1.

    Subcellular locationi

    Nucleus 5 Publications
    Note: Localizes to kinechores during late pachytene.

    GO - Cellular componenti

    1. condensed nuclear chromosome kinetochore Source: SGD
    2. monopolin complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302Monopolin complex subunit MAM1PRO_0000202645Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PeptideAtlasiP40065.

    Expressioni

    Gene expression databases

    GenevestigatoriP40065.

    Interactioni

    Subunit structurei

    Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore during late pachytene.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CSM1P256518EBI-22643,EBI-22001
    HRR25P292953EBI-22643,EBI-8536

    Protein-protein interaction databases

    BioGridi36852. 19 interactions.
    DIPiDIP-5402N.
    IntActiP40065. 3 interactions.
    MINTiMINT-498700.
    STRINGi4932.YER106W.

    Structurei

    Secondary structure

    1
    302
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi233 – 2364
    Helixi247 – 2559

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EMCX-ray3.05C221-290[»]
    ProteinModelPortaliP40065.
    SMRiP40065. Positions 223-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG70991.
    KOiK12774.
    OrthoDBiEOG7P2Z5N.

    Family and domain databases

    InterProiIPR018847. Monopolin_cplx_su_Mam1.
    [Graphical view]
    PfamiPF10434. MAM1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40065-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREKRTISNK DTNYLKFPNK LQRYSRFLSR KISNTSPEKQ PKKNIKEHCL    50
    SSYHKEHSVK PKQNSGNVAA KEDKDTQHLQ NNVANEEATE CLTRSNLKKL 100
    QEKIFDRELN DIACDHCLCS TENRRDIKYS RLWFLFELEM SENWNENLRL 150
    SCYNKYVYSA IDESWKMENI LLKEQEKHYE YFPIGQLLIP NNIDYTNKQK 200
    RKENIEDLTI EIDSIIETNH QKKRFLPQSV LIKREDEIAF DDFHLDARKV 250
    LNDLSATSEN PFSSSPNTKK IKSKGKTLEV VPKKKNKKII GALERKLHID 300
    EN 302
    Length:302
    Mass (Da):35,753
    Last modified:February 1, 1995 - v1
    Checksum:i64E467A38EB831FF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti202 – 2021K → E in AAT92900. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64661.1.
    AY692881 Genomic DNA. Translation: AAT92900.1.
    BK006939 Genomic DNA. Translation: DAA07767.1.
    PIRiS50609.
    RefSeqiNP_011032.1. NM_001178997.1.

    Genome annotation databases

    EnsemblFungiiYER106W; YER106W; YER106W.
    GeneIDi856843.
    KEGGisce:YER106W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64661.1 .
    AY692881 Genomic DNA. Translation: AAT92900.1 .
    BK006939 Genomic DNA. Translation: DAA07767.1 .
    PIRi S50609.
    RefSeqi NP_011032.1. NM_001178997.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EMC X-ray 3.05 C 221-290 [» ]
    ProteinModelPortali P40065.
    SMRi P40065. Positions 223-263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36852. 19 interactions.
    DIPi DIP-5402N.
    IntActi P40065. 3 interactions.
    MINTi MINT-498700.
    STRINGi 4932.YER106W.

    Proteomic databases

    PeptideAtlasi P40065.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER106W ; YER106W ; YER106W .
    GeneIDi 856843.
    KEGGi sce:YER106W.

    Organism-specific databases

    CYGDi YER106w.
    SGDi S000000908. MAM1.

    Phylogenomic databases

    eggNOGi NOG70991.
    KOi K12774.
    OrthoDBi EOG7P2Z5N.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30271-MONOMER.

    Miscellaneous databases

    NextBioi 983162.

    Gene expression databases

    Genevestigatori P40065.

    Family and domain databases

    InterProi IPR018847. Monopolin_cplx_su_Mam1.
    [Graphical view ]
    Pfami PF10434. MAM1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Functional genomics identifies monopolin: a kinetochore protein required for segregation of homologs during meiosis I."
      Toth A., Rabitsch K.P., Galova M., Schleiffer A., Buonomo S.B.C., Nasmyth K.
      Cell 103:1155-1168(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. Cited for: FUNCTION.
    6. "Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I."
      Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K.
      Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
    7. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
      Enyenihi A.H., Saunders W.S.
      Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I."
      Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K.
      Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    9. "Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation."
      Lee B.H., Amon A.
      Science 300:482-486(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    10. "Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis."
      Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K.
      Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    11. "Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II."
      Petronczki M., Chwalla B., Siomos M.F., Yokobayashi S., Helmhart W., Deutschbauer A.M., Davis R.W., Watanabe Y., Nasmyth K.
      J. Cell Sci. 117:3547-3559(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMAM1_YEAST
    AccessioniPrimary (citable) accession number: P40065
    Secondary accession number(s): D3DM13, E9P8Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3