Monopolin complex subunit MAM1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40065 (MAM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Monopolin complex subunit MAM1

Alternative name(s):
Monopolar microtubule attachment during meiosis 1 protein 1

Gene names

Name:	MAM1
Ordered Locus Names:	YER106W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
Subunit structure	Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore during late pachytene. Ref.6
Subcellular location	Nucleus. Note: Localizes to kinechores during late pachytene. Ref.4 Ref.6 Ref.8 Ref.9 Ref.10
Post-translational modification	Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene. Ref.8 Ref.9

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiotic sister chromatid cohesion involved in meiosis I Inferred from mutant phenotype PubMed 15062096. Source: SGD
Cellular_component	condensed nuclear chromosome kinetochore Inferred from direct assay Ref.4 Ref.9. Source: SGD monopolin complex Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CSM1	P25651	8	EBI-22643,EBI-22001
HRR25	P29295	3	EBI-22643,EBI-8536

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 302

302

Monopolin complex subunit MAM1

PRO_0000202645

Experimental info

Sequence conflict

202

K → E in AAT92900. Ref.3

Secondary structure

302

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P40065 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 64E467A38EB831FF
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FASTA

302

35,753

        10         20         30         40         50         60 
MREKRTISNK DTNYLKFPNK LQRYSRFLSR KISNTSPEKQ PKKNIKEHCL SSYHKEHSVK 

        70         80         90        100        110        120 
PKQNSGNVAA KEDKDTQHLQ NNVANEEATE CLTRSNLKKL QEKIFDRELN DIACDHCLCS 

       130        140        150        160        170        180 
TENRRDIKYS RLWFLFELEM SENWNENLRL SCYNKYVYSA IDESWKMENI LLKEQEKHYE 

       190        200        210        220        230        240 
YFPIGQLLIP NNIDYTNKQK RKENIEDLTI EIDSIIETNH QKKRFLPQSV LIKREDEIAF 

       250        260        270        280        290        300 
DDFHLDARKV LNDLSATSEN PFSSSPNTKK IKSKGKTLEV VPKKKNKKII GALERKLHID 


EN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Functional genomics identifies monopolin: a kinetochore protein required for segregation of homologs during meiosis I." Toth A., Rabitsch K.P., Galova M., Schleiffer A., Buonomo S.B.C., Nasmyth K. Cell 103:1155-1168(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I." Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K. Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
[7]	"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae." Enyenihi A.H., Saunders W.S. Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I." Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K. Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[9]	"Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation." Lee B.H., Amon A. Science 300:482-486(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[10]	"Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis." Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K. Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]	"Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II." Petronczki M., Chwalla B., Siomos M.F., Yokobayashi S., Helmhart W., Deutschbauer A.M., Davis R.W., Watanabe Y., Nasmyth K. J. Cell Sci. 117:3547-3559(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18839 Genomic DNA. Translation: AAB64661.1.
AY692881 Genomic DNA. Translation: AAT92900.1.
BK006939 Genomic DNA. Translation: DAA07767.1.

PIR

S50609.

RefSeq

NP_011032.1. NM_001178997.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4EMC	X-ray	3.05	C	221-290	[»]

ProteinModelPortal

P40065.

SMR

P40065. Positions 223-263.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5402N.

IntAct

P40065. 3 interactions.

MINT

MINT-498700.

STRING

4932.YER106W.

Proteomic databases

PeptideAtlas

P40065.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER106W; YER106W; YER106W.

GeneID

856843.

KEGG

sce:YER106W.

Organism-specific databases

CYGD

YER106w.

SGD

S000000908. MAM1.

Phylogenomic databases

eggNOG

NOG70991.

K12774.

OrthoDB

EOG4PK5JQ.

Gene expression databases

Genevestigator

P40065.

GermOnline

YER106W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018847. Monopolin_cplx_su_Mam1.
[Graphical view]

Pfam

PF10434. MAM1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

983162.

Entry information

Entry name

MAM1_YEAST

Accession

Primary (citable) accession number: P40065
Secondary accession number(s): D3DM13, E9P8Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents