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Protein

Monopolin complex subunit MAM1

Gene

MAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis.8 Publications

GO - Biological processi

  • meiotic chromosome segregation Source: SGD
  • meiotic sister chromatid cohesion involved in meiosis I Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Monopolin complex subunit MAM1
Alternative name(s):
Monopolar microtubule attachment during meiosis 1 protein 1
Gene namesi
Name:MAM1
Ordered Locus Names:YER106W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER106w.
EuPathDBiFungiDB:YER106W.
SGDiS000000908. MAM1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: SGD
  • monopolin complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Monopolin complex subunit MAM1PRO_0000202645Add
BLAST

Post-translational modificationi

Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiP40065.

Interactioni

Subunit structurei

Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore during late pachytene.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSM1P256518EBI-22643,EBI-22001
HRR25P292953EBI-22643,EBI-8536

Protein-protein interaction databases

BioGridi36852. 19 interactions.
DIPiDIP-5402N.
IntActiP40065. 3 interactions.
MINTiMINT-498700.
STRINGi4932.YER106W.

Structurei

Secondary structure

1
302
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi233 – 2364Combined sources
Helixi247 – 2559Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EMCX-ray3.05C221-290[»]
ProteinModelPortaliP40065.
SMRiP40065. Positions 223-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG70991.
InParanoidiP40065.
KOiK12774.
OrthoDBiEOG7P2Z5N.

Family and domain databases

InterProiIPR018847. Monopolin_cplx_su_Mam1.
[Graphical view]
PfamiPF10434. MAM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREKRTISNK DTNYLKFPNK LQRYSRFLSR KISNTSPEKQ PKKNIKEHCL
60 70 80 90 100
SSYHKEHSVK PKQNSGNVAA KEDKDTQHLQ NNVANEEATE CLTRSNLKKL
110 120 130 140 150
QEKIFDRELN DIACDHCLCS TENRRDIKYS RLWFLFELEM SENWNENLRL
160 170 180 190 200
SCYNKYVYSA IDESWKMENI LLKEQEKHYE YFPIGQLLIP NNIDYTNKQK
210 220 230 240 250
RKENIEDLTI EIDSIIETNH QKKRFLPQSV LIKREDEIAF DDFHLDARKV
260 270 280 290 300
LNDLSATSEN PFSSSPNTKK IKSKGKTLEV VPKKKNKKII GALERKLHID

EN
Length:302
Mass (Da):35,753
Last modified:February 1, 1995 - v1
Checksum:i64E467A38EB831FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021K → E in AAT92900 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64661.1.
AY692881 Genomic DNA. Translation: AAT92900.1.
BK006939 Genomic DNA. Translation: DAA07767.1.
PIRiS50609.
RefSeqiNP_011032.1. NM_001178997.1.

Genome annotation databases

EnsemblFungiiYER106W; YER106W; YER106W.
GeneIDi856843.
KEGGisce:YER106W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64661.1.
AY692881 Genomic DNA. Translation: AAT92900.1.
BK006939 Genomic DNA. Translation: DAA07767.1.
PIRiS50609.
RefSeqiNP_011032.1. NM_001178997.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EMCX-ray3.05C221-290[»]
ProteinModelPortaliP40065.
SMRiP40065. Positions 223-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36852. 19 interactions.
DIPiDIP-5402N.
IntActiP40065. 3 interactions.
MINTiMINT-498700.
STRINGi4932.YER106W.

Proteomic databases

PeptideAtlasiP40065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER106W; YER106W; YER106W.
GeneIDi856843.
KEGGisce:YER106W.

Organism-specific databases

CYGDiYER106w.
EuPathDBiFungiDB:YER106W.
SGDiS000000908. MAM1.

Phylogenomic databases

eggNOGiNOG70991.
InParanoidiP40065.
KOiK12774.
OrthoDBiEOG7P2Z5N.

Enzyme and pathway databases

BioCyciYEAST:G3O-30271-MONOMER.

Miscellaneous databases

NextBioi983162.
PROiP40065.

Family and domain databases

InterProiIPR018847. Monopolin_cplx_su_Mam1.
[Graphical view]
PfamiPF10434. MAM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Functional genomics identifies monopolin: a kinetochore protein required for segregation of homologs during meiosis I."
    Toth A., Rabitsch K.P., Galova M., Schleiffer A., Buonomo S.B.C., Nasmyth K.
    Cell 103:1155-1168(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION.
  6. "Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I."
    Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K.
    Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
  7. "Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
    Enyenihi A.H., Saunders W.S.
    Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I."
    Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K.
    Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  9. "Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation."
    Lee B.H., Amon A.
    Science 300:482-486(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  10. "Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis."
    Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K.
    Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II."
    Petronczki M., Chwalla B., Siomos M.F., Yokobayashi S., Helmhart W., Deutschbauer A.M., Davis R.W., Watanabe Y., Nasmyth K.
    J. Cell Sci. 117:3547-3559(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMAM1_YEAST
AccessioniPrimary (citable) accession number: P40065
Secondary accession number(s): D3DM13, E9P8Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.