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P40065 (MAM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monopolin complex subunit MAM1
Alternative name(s):
Monopolar microtubule attachment during meiosis 1 protein 1
Gene names
Name:MAM1
Ordered Locus Names:YER106W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11

Subunit structure

Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore during late pachytene. Ref.6

Subcellular location

Nucleus. Note: Localizes to kinechores during late pachytene. Ref.4 Ref.6 Ref.8 Ref.9 Ref.10

Post-translational modification

Phosphorylated by CDC5. This phosphorylation is required for the location to the kinetochores during late pachytene. Ref.8 Ref.9

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CSM1P256518EBI-22643,EBI-22001
HRR25P292953EBI-22643,EBI-8536

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Monopolin complex subunit MAM1
PRO_0000202645

Experimental info

Sequence conflict2021K → E in AAT92900. Ref.3

Secondary structure

..... 302
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40065 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 64E467A38EB831FF

FASTA30235,753
        10         20         30         40         50         60 
MREKRTISNK DTNYLKFPNK LQRYSRFLSR KISNTSPEKQ PKKNIKEHCL SSYHKEHSVK 

        70         80         90        100        110        120 
PKQNSGNVAA KEDKDTQHLQ NNVANEEATE CLTRSNLKKL QEKIFDRELN DIACDHCLCS 

       130        140        150        160        170        180 
TENRRDIKYS RLWFLFELEM SENWNENLRL SCYNKYVYSA IDESWKMENI LLKEQEKHYE 

       190        200        210        220        230        240 
YFPIGQLLIP NNIDYTNKQK RKENIEDLTI EIDSIIETNH QKKRFLPQSV LIKREDEIAF 

       250        260        270        280        290        300 
DDFHLDARKV LNDLSATSEN PFSSSPNTKK IKSKGKTLEV VPKKKNKKII GALERKLHID 


EN 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Functional genomics identifies monopolin: a kinetochore protein required for segregation of homologs during meiosis I."
Toth A., Rabitsch K.P., Galova M., Schleiffer A., Buonomo S.B.C., Nasmyth K.
Cell 103:1155-1168(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"A screen for genes required for meiosis and spore formation based on whole-genome expression."
Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K.
Curr. Biol. 11:1001-1009(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I."
Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K.
Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
[7]"Large-scale functional genomic analysis of sporulation and meiosis in Saccharomyces cerevisiae."
Enyenihi A.H., Saunders W.S.
Genetics 163:47-54(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I."
Clyne R.K., Katis V.L., Jessop L., Benjamin K.R., Herskowitz I., Lichten M., Nasmyth K.
Nat. Cell Biol. 5:480-485(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[9]"Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation."
Lee B.H., Amon A.
Science 300:482-486(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[10]"Spo13 facilitates monopolin recruitment to kinetochores and regulates maintenance of centromeric cohesion during yeast meiosis."
Katis V.L., Matos J., Mori S., Shirahige K., Zachariae W., Nasmyth K.
Curr. Biol. 14:2183-2196(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II."
Petronczki M., Chwalla B., Siomos M.F., Yokobayashi S., Helmhart W., Deutschbauer A.M., Davis R.W., Watanabe Y., Nasmyth K.
J. Cell Sci. 117:3547-3559(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18839 Genomic DNA. Translation: AAB64661.1.
AY692881 Genomic DNA. Translation: AAT92900.1.
BK006939 Genomic DNA. Translation: DAA07767.1.
PIRS50609.
RefSeqNP_011032.1. NM_001178997.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EMCX-ray3.05C221-290[»]
ProteinModelPortalP40065.
SMRP40065. Positions 223-263.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36852. 19 interactions.
DIPDIP-5402N.
IntActP40065. 3 interactions.
MINTMINT-498700.
STRING4932.YER106W.

Proteomic databases

PeptideAtlasP40065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER106W; YER106W; YER106W.
GeneID856843.
KEGGsce:YER106W.

Organism-specific databases

CYGDYER106w.
SGDS000000908. MAM1.

Phylogenomic databases

eggNOGNOG70991.
KOK12774.
OrthoDBEOG7P2Z5N.

Enzyme and pathway databases

BioCycYEAST:G3O-30271-MONOMER.

Gene expression databases

GenevestigatorP40065.

Family and domain databases

InterProIPR018847. Monopolin_cplx_su_Mam1.
[Graphical view]
PfamPF10434. MAM1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983162.

Entry information

Entry nameMAM1_YEAST
AccessionPrimary (citable) accession number: P40065
Secondary accession number(s): D3DM13, E9P8Y3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references