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P40064 (NU157_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoporin NUP157
Alternative name(s):
Nuclear pore protein NUP157
Gene names
Name:NUP157
Ordered Locus Names:YER105C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Ref.5

Subunit structure

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP157 may be part of a NPC subcomplex containing NUP53, NUP170, and NUP59. In addition it may bind to MAD2. Ref.4

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.

Miscellaneous

Present with 4420 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the non-repetitive/WGA-negative nucleoporin family.

Ontologies

Keywords
   Biological processmRNA transport
Protein transport
Translocation
Transport
   Cellular componentMembrane
Nuclear pore complex
Nucleus
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA transport

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic spindle assembly checkpoint

Inferred from mutant phenotype Ref.5. Source: SGD

nuclear pore complex assembly

Inferred from genetic interaction PubMed 19414608. Source: SGD

nucleocytoplasmic transport

Inferred by curator Ref.4. Source: SGD

posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery

Inferred from mutant phenotype PubMed 20932479. Source: SGD

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery

Inferred from mutant phenotype PubMed 20098417PubMed 20932479. Source: SGD

   Cellular_componentnuclear membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear pore

Inferred from direct assay Ref.4. Source: SGD

nuclear pore inner ring

Inferred from direct assay PubMed 18046406. Source: SGD

   Molecular_functionDNA binding

Inferred from direct assay PubMed 24062435. Source: SGD

RNA binding

Inferred from direct assay PubMed 24062435. Source: SGD

structural constituent of nuclear pore

Inferred from genetic interaction PubMed 19414608. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13911391Nucleoporin NUP157
PRO_0000204848

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10
Modified residue10341Phosphoserine Ref.9

Secondary structure

......................................................................................................................... 1391
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40064 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: CB47CEE27AB4FCE3

FASTA1,391156,649
        10         20         30         40         50         60 
MYSTPLKKRI DYDRETFTAS ASLGGNRLRN RPRDDQNNGK PNLSSRSFLS ERKTRKDVLN 

        70         80         90        100        110        120 
KYGEAGNTIE SELRDVTTHV KISGLTSSEP LQLASEFVQD LSFRDRNTPI LDNPDYYSKG 

       130        140        150        160        170        180 
LDYNFSDEVG GLGAFTPFQR QQVTNIPDEV LSQVSNTEIK SDMGIFLELN YCWITSDNKL 

       190        200        210        220        230        240 
ILWNINNSSE YHCIDEIEHT ILKVKLVKPS PNTFVSSVEN LLIVATLFDI YILTISFNDR 

       250        260        270        280        290        300 
THELNIFNTG LKVNVTGFNV SNIISYERTG QIFFTGATDG VNVWELQYNC SENLFNSKSN 

       310        320        330        340        350        360 
KICLTKSNLA NLLPTKLIPS IPGGKLIQKV LEGDAGTEEE TISQLEVDQS RGVLHTLSTK 

       370        380        390        400        410        420 
SIVRSYLITS NGLVGPVLID AAHIRRGMNA LGVKNSPLLS NRAFKIAKIV SISMCENNDL 

       430        440        450        460        470        480 
FLAVITTTGV RLYFKGSISR RSIGSLKLDS VKFPPTSISS SLEQNKSFII GHHPLNTHDT 

       490        500        510        520        530        540 
GPLSTQKASS TYINTTCAST IISPGIYFTC VRKRANSGEL SKGITNKALL ENKEEHKLYV 

       550        560        570        580        590        600 
SAPDYGILKN YGKYVENTAL LDTTDEIKEI VPLTRSFNYT STPQGYANVF ASQYSAEPLK 

       610        620        630        640        650        660 
VAVLTSNALE IYCYRTPDEV FESLIENPLP FIHSYGLSEA CSTALYLACK FNKSEHIKSS 

       670        680        690        700        710        720 
ALAFFSAGIP GVVEIKPKSS RESGSVPPIS QNLFDKSGEC DGIVLSPRFY GSALLITRLF 

       730        740        750        760        770        780 
SQIWEERVFV FKRASKTEKM DAFGISITRP QVEYYLSSIS VLADFFNIHR PSFVSFVPPK 

       790        800        810        820        830        840 
GSNAITASDA ESIAMNALIL LINSIKDALS LINVFYEDID AFKSLLNTLM GAGGVYDSKT 

       850        860        870        880        890        900 
REYFFDLKFH DLFTPNAKTK QLIKEILIEV VNANIASGTS ADYIVNVLKE RFGSFCHSAD 

       910        920        930        940        950        960 
ILCYRAGEHL EAAQKFEMID SKISRNHLDT AIDLYERCAE NIELCELRRV VDIMVKLNYQ 

       970        980        990       1000       1010       1020 
PKTVGFLLRF ADKIDKGNQA QEYVSRGCNT ADPRKVFYDK RINVYTLIFE IVKSVDDYTS 

      1030       1040       1050       1060       1070       1080 
IEQSPSIANI SIFSPASSLK KRVYSVIMNS NNRFFHYCFY DWLVANKRQD YLLRLDSQFV 

      1090       1100       1110       1120       1130       1140 
LPYLKERAEK SLEISNLLWF YLFKEEHFLE AADVLYALAS SDFDLKLSER IECLARANGL 

      1150       1160       1170       1180       1190       1200 
CDSSTSFDQK PALVQLSENI HELFDIASIQ DDLLNLVRNE TRIDEDYRKQ LTLKLNGRVL 

      1210       1220       1230       1240       1250       1260 
PLSDLFNDCA DPLDYYEIKL RIFKVSQFKD EKVIQGEWNR LLDSMKNAPS PDVGSVGQES 

      1270       1280       1290       1300       1310       1320 
FLSSISNTLI RIGKTTRDTD VVFPVHFLMN KILESFIDKS SAADGSVCSM FLLAGVSHLK 

      1330       1340       1350       1360       1370       1380 
LYYILSRIIE NSEGNVELAK KEMVWLIKDW YQSDSDLRGS IAPEQIKKLE KYDPNTDPVQ 

      1390 
DYVKDRHHGL K 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p."
Aitchison J.D., Rout M.P., Marelli M., Blobel G., Wozniak R.W.
J. Cell Biol. 131:1133-1148(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 1016-1030 AND 1349-1367.
[4]"The yeast nuclear pore complex: composition, architecture, and transport mechanism."
Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[5]"The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint."
Iouk T., Kerscher O., Scott R.J., Basrai M.A., Wozniak R.W.
J. Cell Biol. 159:807-819(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NUP170 AND NUP53.
[6]"A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae."
Uetz P., Giot L., Cagney G., Mansfield T.A., Judson R.S., Knight J.R., Lockshon D., Narayan V., Srinivasan M., Pochart P., Qureshi-Emili A., Li Y., Godwin B., Conover D., Kalbfleisch T., Vijayadamodar G., Yang M., Johnston M., Fields S., Rothberg J.M.
Nature 403:623-627(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAD2 AND NUP53.
[7]"Peering through the pore: nuclear pore complex structure, assembly, and function."
Suntharalingam M., Wente S.R.
Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1034, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18839 Genomic DNA. Translation: AAB64660.1.
BK006939 Genomic DNA. Translation: DAA07766.1.
PIRS50608.
RefSeqNP_011031.1. NM_001178996.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4MHCX-ray2.40A70-893[»]
ProteinModelPortalP40064.
SMRP40064. Positions 88-892.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36851. 62 interactions.
DIPDIP-846N.
IntActP40064. 16 interactions.
MINTMINT-385200.
STRING4932.YER105C.

Protein family/group databases

TCDB1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

PaxDbP40064.
PeptideAtlasP40064.

Protocols and materials databases

DNASU856842.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER105C; YER105C; YER105C.
GeneID856842.
KEGGsce:YER105C.

Organism-specific databases

CYGDYER105c.
SGDS000000907. NUP157.

Phylogenomic databases

eggNOGCOG5308.
GeneTreeENSGT00390000016532.
HOGENOMHOG000246581.
OMANDRTHEL.
OrthoDBEOG7GXPKT.

Enzyme and pathway databases

BioCycYEAST:G3O-30270-MONOMER.

Gene expression databases

GenevestigatorP40064.

Family and domain databases

InterProIPR007187. Nucleoporin_Nup133/Nup155_C.
IPR014908. Nucleoporin_Nup133/Nup155_N.
IPR004870. Nucleoporin_Nup155.
[Graphical view]
PANTHERPTHR10350. PTHR10350. 1 hit.
PfamPF03177. Nucleoporin_C. 1 hit.
PF08801. Nucleoporin_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio983159.

Entry information

Entry nameNU157_YEAST
AccessionPrimary (citable) accession number: P40064
Secondary accession number(s): D3DM12
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references