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P40061

- TSC11_YEAST

UniProt

P40061 - TSC11_YEAST

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Protein
Target of rapamycin complex 2 subunit TSC11
Gene
TSC11, AVO3, YER093C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. TSC11 may exert its functions through two distinct mechanisms, one mediated by AVO1 and the other mediated by AVO2 and SLM1.1 Publication

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
  2. protein binding Source: IntAct

GO - Biological processi

  1. TOR signaling Source: SGD
  2. establishment or maintenance of actin cytoskeleton polarity Source: SGD
  3. fungal-type cell wall organization Source: SGD
  4. regulation of cell growth Source: SGD
  5. sphingolipid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciYEAST:G3O-30260-MONOMER.
ReactomeiREACT_188925. CD28 dependent PI3K/Akt signaling.
REACT_188931. PIP3 activates AKT signaling.
REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit TSC11
Short name:
TORC2 subunit TSC11
Alternative name(s):
Adheres voraciously to TOR2 protein 3
Temperature-sensitive CSG2 suppressor protein 11
Gene namesi
Name:TSC11
Synonyms:AVO3
Ordered Locus Names:YER093C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER093c.
SGDiS000000895. TSC11.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side 1 Publication

GO - Cellular componenti

  1. TORC2 complex Source: SGD
  2. plasma membrane Source: UniProtKB-SubCell
  3. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14301430Target of rapamycin complex 2 subunit TSC11
PRO_0000202640Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine2 Publications
Modified residuei81 – 811Phosphoserine1 Publication
Modified residuei84 – 841Phosphoserine1 Publication
Modified residuei87 – 871Phosphoserine1 Publication
Modified residuei141 – 1411Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by TOR2; when part of TORC2.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40061.
PaxDbiP40061.
PeptideAtlasiP40061.

Expressioni

Gene expression databases

GenevestigatoriP40061.

Interactioni

Subunit structurei

The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. TSC11 binds to the N-terminal HEAT repeat region in TOR2 and is required for TORC2 integrity by tethering AVO1 and AVO2 to the complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LST8P413182EBI-22621,EBI-28598
TOR2P326005EBI-22621,EBI-19385

Protein-protein interaction databases

BioGridi36838. 180 interactions.
DIPiDIP-4957N.
IntActiP40061. 10 interactions.
MINTiMINT-486144.
STRINGi4932.YER093C.

Structurei

3D structure databases

ProteinModelPortaliP40061.
SMRiP40061. Positions 627-658, 1148-1215.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini995 – 1100106N-terminal Ras-GEF
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili91 – 18090 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the pianissimo family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG305760.
GeneTreeiENSGT00390000002096.
HOGENOMiHOG000141815.
OMAiGRNDTYV.
OrthoDBiEOG71VT21.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR028268. Pianissimo_fam.
IPR028267. Pianissimo_N.
IPR029453. Rictor_IV.
IPR029451. RICTOR_M.
IPR029452. RICTOR_V.
[Graphical view]
PANTHERiPTHR13298. PTHR13298. 1 hit.
PfamiPF14663. RasGEF_N_2. 1 hit.
PF14666. RICTOR_M. 1 hit.
PF14664. RICTOR_N. 1 hit.
PF14668. RICTOR_V. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequencei

Sequence statusi: Complete.

P40061-1 [UniParc]FASTAAdd to Basket

« Hide

MSIPHSAKQS SPLSSRRRSV TNTTPLLTPR HSRDNSSTQI SSAKNITSSS     50
PSTITNESSK RNKQNLVLST SFISTKRLEN SAPSPTSPLM ARRTRSTMTK 100
ALLNLKAEIN NQYQELARLR KKKDDIEHLR DSTISDIYSG SYSTNHLQKH 150
SMRIRANTQL REIDNSIKRV EKHIFDLKQQ FDKKRQRSLT TSSSIKADVG 200
SIRNDDGQNN DSEELGDHDS LTDQVTLDDE YLTTPTSGTE RNSQQNLNRN 250
STVNSRNNEN HSTLSIPDLD GSNKVNLTGD TEKDLGDLEN ENQIFTSTTT 300
EAATWLVSDY MQSFQEKNVN PDFIAQKANG LVTLLKEHSE IRKDLVLTSF 350
MSSIQNLLLN GNKLIAASAY RVCRYLINSS IFIDELLELR LDAFIIISLA 400
KDNSFQIERE QALKMVRRFI EYNNGVTQGI MQAIISCVEK PEDSLRHMAL 450
ETLLELCFVA PEMVKECRGM RVIEGFLQDY TSFSLASVIL DTILQLMATH 500
KTRQHFLEDF NVSVLTTVFS DTNTKSNVNV EKMQNASTLI SITLNSYNGF 550
MLFSNNNFKP LKQLVSFFQI PICAQYLIDI FLDVLKIKPL PYKPRGRHSH 600
SFKPIPSQYY KECMSVNQRL ALIVLILENS EFVPHLLELL NEEDRDDHLV 650
AKGRYLLTEY FNLRMNLVDK KYTSVSKPIY KENFTYVNET FQFKKIAYKM 700
NRNRNTIGMS GIDYAQNIKS FSKNIKENTL LREVDDFRFR RMVYDSKVLQ 750
TKDFTRWNWN IINELLEGPL LNKKQLEELV KSTKFIRRLL VFYRPLRLRF 800
SNVNKGAKLS QKYVQVGCQF FKTLTATPEG MKILMDDTKI IPQLASLMFR 850
AMEGNISGNI FNKNKLREKI IFGYFKFIGI LTQSKNGVHI LTRWNFFTVI 900
YKMFQFESKL GLEFLLLTIP ELDLKYSSHC RVIIGKALVV ANEKVRIEAT 950
KHIGDKLKEL LSTKESDLKL KANKVKLQQF KMEMLTRQLY DLSPSVVAVA 1000
DQALYECIVA GNGSEELGTS FRMFLNQMVF IRSPILFELL SRPYGFQLLN 1050
EINFVKEERD SWLSKKNIEY VHIVEEFLKK NESINAKSLT FQQKSRLPLH 1100
FYESLTKTED GILLLSQTGD LVTFMNVIKK YVNGNNMATV ENAKEILDLK 1150
AALWCVGFIG STELGIGLLD NYSLVEDIIE VAYNASVTSV RFTAFYVLGL 1200
ISMTREGCEI LDEMGWNCCV SVQDEPIGIA LPNRLDRFLS YNEHKWSAFG 1250
EYSDEMIVFN KSDGDLIEKC LPIEFDLDKL LKEKDTAENP LNEKIITNKY 1300
DNDITSQTIT VSGENSSLFA NEGLSSPYVT QYRNDDDSIE SKVLHIVSQL 1350
GNHILSNHAV KEITEINNKY GPRLFENEKM FFKVFNMMSK YRFKPHVRKF 1400
LCGLFINNRA LENVIRHDNK RDKRPANFTR 1430
Length:1,430
Mass (Da):164,368
Last modified:February 1, 1995 - v1
Checksum:i07BB5D75BA204E50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18839 Genomic DNA. Translation: AAB64648.1.
U32508 Genomic DNA. Translation: AAB60298.1.
BK006939 Genomic DNA. Translation: DAA07754.1.
PIRiS50596.
RefSeqiNP_011018.1. NM_001178984.1.

Genome annotation databases

EnsemblFungiiYER093C; YER093C; YER093C.
GeneIDi856828.
KEGGisce:YER093C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18839 Genomic DNA. Translation: AAB64648.1 .
U32508 Genomic DNA. Translation: AAB60298.1 .
BK006939 Genomic DNA. Translation: DAA07754.1 .
PIRi S50596.
RefSeqi NP_011018.1. NM_001178984.1.

3D structure databases

ProteinModelPortali P40061.
SMRi P40061. Positions 627-658, 1148-1215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36838. 180 interactions.
DIPi DIP-4957N.
IntActi P40061. 10 interactions.
MINTi MINT-486144.
STRINGi 4932.YER093C.

Proteomic databases

MaxQBi P40061.
PaxDbi P40061.
PeptideAtlasi P40061.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER093C ; YER093C ; YER093C .
GeneIDi 856828.
KEGGi sce:YER093C.

Organism-specific databases

CYGDi YER093c.
SGDi S000000895. TSC11.

Phylogenomic databases

eggNOGi NOG305760.
GeneTreei ENSGT00390000002096.
HOGENOMi HOG000141815.
OMAi GRNDTYV.
OrthoDBi EOG71VT21.

Enzyme and pathway databases

BioCyci YEAST:G3O-30260-MONOMER.
Reactomei REACT_188925. CD28 dependent PI3K/Akt signaling.
REACT_188931. PIP3 activates AKT signaling.
REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

Miscellaneous databases

NextBioi 983121.

Gene expression databases

Genevestigatori P40061.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR028268. Pianissimo_fam.
IPR028267. Pianissimo_N.
IPR029453. Rictor_IV.
IPR029451. RICTOR_M.
IPR029452. RICTOR_V.
[Graphical view ]
PANTHERi PTHR13298. PTHR13298. 1 hit.
Pfami PF14663. RasGEF_N_2. 1 hit.
PF14666. RICTOR_M. 1 hit.
PF14664. RICTOR_N. 1 hit.
PF14668. RICTOR_V. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 4 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Structure and regulation of MET6, the vitamin B12-independent methionine synthase gene of Saccharomyces cerevisiae."
    Korch C., Mountain H.A., Wenzlau J.M.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 967-1430.
    Strain: ATCC 204508 / S288c.
  4. "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
    Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N.
    Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae."
    Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T.
    Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Molecular organization of target of rapamycin complex 2."
    Wullschleger S., Loewith R., Oppliger W., Hall M.N.
    J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH TOR2, PHOSPHORYLATION.
  8. "Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex."
    Ho H.-L., Shiau Y.-S., Chen M.-Y.
    Curr. Genet. 47:273-288(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-81; SER-87 AND SER-141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTSC11_YEAST
AccessioniPrimary (citable) accession number: P40061
Secondary accession number(s): D3DM00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1840 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi