Target of rapamycin complex 2 subunit TSC11 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P40061 (TSC11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Target of rapamycin complex 2 subunit TSC11
Short name=TORC2 subunit TSC11

Alternative name(s):
Adheres voraciously to TOR2 protein 3
Temperature-sensitive CSG2 suppressor protein 11

Gene names

Name:	TSC11
Synonyms:	AVO3
Ordered Locus Names:	YER093C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. TSC11 may exert its functions through two distinct mechanisms, one mediated by AVO1 and the other mediated by AVO2 and SLM1. Ref.8
Subunit structure	The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. TSC11 binds to the N-terminal HEAT repeat region in TOR2 and is required for TORC2 integrity by tethering AVO1 and AVO2 to the complex. Ref.4 Ref.5 Ref.7
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side Ref.5.
Post-translational modification	Phosphorylated by TOR2; when part of TORC2. Ref.7
Miscellaneous	Present with 1840 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the pianissimo family. Contains 1 N-terminal Ras-GEF domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Vacuole
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	TOR signaling cascade Inferred by curator Ref.4. Source: SGD establishment or maintenance of actin cytoskeleton polarity Inferred from mutant phenotype Ref.8. Source: SGD fungal-type cell wall organization Inferred from mutant phenotype Ref.8. Source: SGD regulation of cell growth Inferred from physical interaction Ref.4. Source: SGD sphingolipid biosynthetic process Inferred from genetic interaction PubMed 9804843. Source: SGD
Cellular_component	TORC2 complex Inferred from physical interaction Ref.4. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LST8	P41318	2	EBI-22621,EBI-28598
TOR2	P32600	5	EBI-22621,EBI-19385

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1430

1430

Target of rapamycin complex 2 subunit TSC11

PRO_0000202640

Regions

Domain

995 – 1100

106

N-terminal Ras-GEF

Coiled coil

91 – 180

Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphoserine Ref.10

Modified residue

Phosphothreonine Ref.9 Ref.10

Modified residue

Phosphoserine Ref.9 Ref.10

Modified residue

166

Phosphoserine Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P40061 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 07BB5D75BA204E50
Show »

FASTA

1,430

164,368

        10         20         30         40         50         60 
MSIPHSAKQS SPLSSRRRSV TNTTPLLTPR HSRDNSSTQI SSAKNITSSS PSTITNESSK 

        70         80         90        100        110        120 
RNKQNLVLST SFISTKRLEN SAPSPTSPLM ARRTRSTMTK ALLNLKAEIN NQYQELARLR 

       130        140        150        160        170        180 
KKKDDIEHLR DSTISDIYSG SYSTNHLQKH SMRIRANTQL REIDNSIKRV EKHIFDLKQQ 

       190        200        210        220        230        240 
FDKKRQRSLT TSSSIKADVG SIRNDDGQNN DSEELGDHDS LTDQVTLDDE YLTTPTSGTE 

       250        260        270        280        290        300 
RNSQQNLNRN STVNSRNNEN HSTLSIPDLD GSNKVNLTGD TEKDLGDLEN ENQIFTSTTT 

       310        320        330        340        350        360 
EAATWLVSDY MQSFQEKNVN PDFIAQKANG LVTLLKEHSE IRKDLVLTSF MSSIQNLLLN 

       370        380        390        400        410        420 
GNKLIAASAY RVCRYLINSS IFIDELLELR LDAFIIISLA KDNSFQIERE QALKMVRRFI 

       430        440        450        460        470        480 
EYNNGVTQGI MQAIISCVEK PEDSLRHMAL ETLLELCFVA PEMVKECRGM RVIEGFLQDY 

       490        500        510        520        530        540 
TSFSLASVIL DTILQLMATH KTRQHFLEDF NVSVLTTVFS DTNTKSNVNV EKMQNASTLI 

       550        560        570        580        590        600 
SITLNSYNGF MLFSNNNFKP LKQLVSFFQI PICAQYLIDI FLDVLKIKPL PYKPRGRHSH 

       610        620        630        640        650        660 
SFKPIPSQYY KECMSVNQRL ALIVLILENS EFVPHLLELL NEEDRDDHLV AKGRYLLTEY 

       670        680        690        700        710        720 
FNLRMNLVDK KYTSVSKPIY KENFTYVNET FQFKKIAYKM NRNRNTIGMS GIDYAQNIKS 

       730        740        750        760        770        780 
FSKNIKENTL LREVDDFRFR RMVYDSKVLQ TKDFTRWNWN IINELLEGPL LNKKQLEELV 

       790        800        810        820        830        840 
KSTKFIRRLL VFYRPLRLRF SNVNKGAKLS QKYVQVGCQF FKTLTATPEG MKILMDDTKI 

       850        860        870        880        890        900 
IPQLASLMFR AMEGNISGNI FNKNKLREKI IFGYFKFIGI LTQSKNGVHI LTRWNFFTVI 

       910        920        930        940        950        960 
YKMFQFESKL GLEFLLLTIP ELDLKYSSHC RVIIGKALVV ANEKVRIEAT KHIGDKLKEL 

       970        980        990       1000       1010       1020 
LSTKESDLKL KANKVKLQQF KMEMLTRQLY DLSPSVVAVA DQALYECIVA GNGSEELGTS 

      1030       1040       1050       1060       1070       1080 
FRMFLNQMVF IRSPILFELL SRPYGFQLLN EINFVKEERD SWLSKKNIEY VHIVEEFLKK 

      1090       1100       1110       1120       1130       1140 
NESINAKSLT FQQKSRLPLH FYESLTKTED GILLLSQTGD LVTFMNVIKK YVNGNNMATV 

      1150       1160       1170       1180       1190       1200 
ENAKEILDLK AALWCVGFIG STELGIGLLD NYSLVEDIIE VAYNASVTSV RFTAFYVLGL 

      1210       1220       1230       1240       1250       1260 
ISMTREGCEI LDEMGWNCCV SVQDEPIGIA LPNRLDRFLS YNEHKWSAFG EYSDEMIVFN 

      1270       1280       1290       1300       1310       1320 
KSDGDLIEKC LPIEFDLDKL LKEKDTAENP LNEKIITNKY DNDITSQTIT VSGENSSLFA 

      1330       1340       1350       1360       1370       1380 
NEGLSSPYVT QYRNDDDSIE SKVLHIVSQL GNHILSNHAV KEITEINNKY GPRLFENEKM 

      1390       1400       1410       1420       1430 
FFKVFNMMSK YRFKPHVRKF LCGLFINNRA LENVIRHDNK RDKRPANFTR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Structure and regulation of MET6, the vitamin B12-independent methionine synthase gene of Saccharomyces cerevisiae." Korch C., Mountain H.A., Wenzlau J.M. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 967-1430. Strain: ATCC 204508 / S288c.
[4]	"Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control." Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N. Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN TORC2, MASS SPECTROMETRY.
[5]	"Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae." Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T. Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, MASS SPECTROMETRY.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Molecular organization of target of rapamycin complex 2." Wullschleger S., Loewith R., Oppliger W., Hall M.N. J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, INTERACTION WITH TOR2, PHOSPHORYLATION.
[8]	"Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex." Ho H.-L., Shiau Y.-S., Chen M.-Y. Curr. Genet. 47:273-288(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86 AND SER-87, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-50; SER-84; THR-86; SER-87 AND SER-166, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18839 Genomic DNA. Translation: AAB64648.1. U32508 Genomic DNA. Translation: AAB60298.1. BK006939 Genomic DNA. Translation: DAA07754.1.
PIR	S50596.
RefSeq	NP_011018.1. NM_001178984.1.
3D structure databases
ProteinModelPortal	P40061.
SMR	P40061. Positions 627-658, 981-1007.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4957N.
IntAct	P40061. 10 interactions.
MINT	MINT-486144.
STRING	4932.YER093C.
Proteomic databases
PaxDb	P40061.
PeptideAtlas	P40061.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER093C; YER093C; YER093C.
GeneID	856828.
KEGG	sce:YER093C.
Organism-specific databases
CYGD	YER093c.
SGD	S000000895. TSC11.
Phylogenomic databases
eggNOG	NOG305760.
GeneTree	ENSGT00390000002096.
HOGENOM	HOG000141815.
OrthoDB	EOG4STWCZ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30260-MONOMER.
Gene expression databases
Genevestigator	P40061.
GermOnline	YER093C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50212. RASGEF_NTER. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	983121.

Entry information

Entry name

TSC11_YEAST

Accession

Primary (citable) accession number: P40061
Secondary accession number(s): D3DM00

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents