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P40061

- TSC11_YEAST

UniProt

P40061 - TSC11_YEAST

Protein

Target of rapamycin complex 2 subunit TSC11

Gene

TSC11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Essential component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway. TSC11 may exert its functions through two distinct mechanisms, one mediated by AVO1 and the other mediated by AVO2 and SLM1.1 Publication

    GO - Molecular functioni

    1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. establishment or maintenance of actin cytoskeleton polarity Source: SGD
    2. fungal-type cell wall organization Source: SGD
    3. regulation of cell growth Source: SGD
    4. sphingolipid biosynthetic process Source: SGD
    5. TOR signaling Source: SGD

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30260-MONOMER.
    ReactomeiREACT_188925. CD28 dependent PI3K/Akt signaling.
    REACT_188931. PIP3 activates AKT signaling.
    REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Target of rapamycin complex 2 subunit TSC11
    Short name:
    TORC2 subunit TSC11
    Alternative name(s):
    Adheres voraciously to TOR2 protein 3
    Temperature-sensitive CSG2 suppressor protein 11
    Gene namesi
    Name:TSC11
    Synonyms:AVO3
    Ordered Locus Names:YER093C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER093c.
    SGDiS000000895. TSC11.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Vacuole membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell
    2. TORC2 complex Source: SGD
    3. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14301430Target of rapamycin complex 2 subunit TSC11PRO_0000202640Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Phosphoserine3 Publications
    Modified residuei81 – 811Phosphoserine2 Publications
    Modified residuei84 – 841Phosphoserine2 Publications
    Modified residuei87 – 871Phosphoserine2 Publications
    Modified residuei141 – 1411Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by TOR2; when part of TORC2.4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40061.
    PaxDbiP40061.
    PeptideAtlasiP40061.

    Expressioni

    Gene expression databases

    GenevestigatoriP40061.

    Interactioni

    Subunit structurei

    The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. TSC11 binds to the N-terminal HEAT repeat region in TOR2 and is required for TORC2 integrity by tethering AVO1 and AVO2 to the complex.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LST8P413182EBI-22621,EBI-28598
    TOR2P326005EBI-22621,EBI-19385

    Protein-protein interaction databases

    BioGridi36838. 180 interactions.
    DIPiDIP-4957N.
    IntActiP40061. 10 interactions.
    MINTiMINT-486144.
    STRINGi4932.YER093C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40061.
    SMRiP40061. Positions 627-658, 1148-1215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini995 – 1100106N-terminal Ras-GEFAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili91 – 18090Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the pianissimo family.Curated
    Contains 1 N-terminal Ras-GEF domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG305760.
    GeneTreeiENSGT00390000002096.
    HOGENOMiHOG000141815.
    OMAiGRNDTYV.
    OrthoDBiEOG71VT21.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR028268. Pianissimo_fam.
    IPR028267. Pianissimo_N.
    IPR029453. Rictor_IV.
    IPR029451. RICTOR_M.
    IPR029452. RICTOR_V.
    [Graphical view]
    PANTHERiPTHR13298. PTHR13298. 1 hit.
    PfamiPF14663. RasGEF_N_2. 1 hit.
    PF14666. RICTOR_M. 1 hit.
    PF14664. RICTOR_N. 1 hit.
    PF14668. RICTOR_V. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 4 hits.

    Sequencei

    Sequence statusi: Complete.

    P40061-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIPHSAKQS SPLSSRRRSV TNTTPLLTPR HSRDNSSTQI SSAKNITSSS     50
    PSTITNESSK RNKQNLVLST SFISTKRLEN SAPSPTSPLM ARRTRSTMTK 100
    ALLNLKAEIN NQYQELARLR KKKDDIEHLR DSTISDIYSG SYSTNHLQKH 150
    SMRIRANTQL REIDNSIKRV EKHIFDLKQQ FDKKRQRSLT TSSSIKADVG 200
    SIRNDDGQNN DSEELGDHDS LTDQVTLDDE YLTTPTSGTE RNSQQNLNRN 250
    STVNSRNNEN HSTLSIPDLD GSNKVNLTGD TEKDLGDLEN ENQIFTSTTT 300
    EAATWLVSDY MQSFQEKNVN PDFIAQKANG LVTLLKEHSE IRKDLVLTSF 350
    MSSIQNLLLN GNKLIAASAY RVCRYLINSS IFIDELLELR LDAFIIISLA 400
    KDNSFQIERE QALKMVRRFI EYNNGVTQGI MQAIISCVEK PEDSLRHMAL 450
    ETLLELCFVA PEMVKECRGM RVIEGFLQDY TSFSLASVIL DTILQLMATH 500
    KTRQHFLEDF NVSVLTTVFS DTNTKSNVNV EKMQNASTLI SITLNSYNGF 550
    MLFSNNNFKP LKQLVSFFQI PICAQYLIDI FLDVLKIKPL PYKPRGRHSH 600
    SFKPIPSQYY KECMSVNQRL ALIVLILENS EFVPHLLELL NEEDRDDHLV 650
    AKGRYLLTEY FNLRMNLVDK KYTSVSKPIY KENFTYVNET FQFKKIAYKM 700
    NRNRNTIGMS GIDYAQNIKS FSKNIKENTL LREVDDFRFR RMVYDSKVLQ 750
    TKDFTRWNWN IINELLEGPL LNKKQLEELV KSTKFIRRLL VFYRPLRLRF 800
    SNVNKGAKLS QKYVQVGCQF FKTLTATPEG MKILMDDTKI IPQLASLMFR 850
    AMEGNISGNI FNKNKLREKI IFGYFKFIGI LTQSKNGVHI LTRWNFFTVI 900
    YKMFQFESKL GLEFLLLTIP ELDLKYSSHC RVIIGKALVV ANEKVRIEAT 950
    KHIGDKLKEL LSTKESDLKL KANKVKLQQF KMEMLTRQLY DLSPSVVAVA 1000
    DQALYECIVA GNGSEELGTS FRMFLNQMVF IRSPILFELL SRPYGFQLLN 1050
    EINFVKEERD SWLSKKNIEY VHIVEEFLKK NESINAKSLT FQQKSRLPLH 1100
    FYESLTKTED GILLLSQTGD LVTFMNVIKK YVNGNNMATV ENAKEILDLK 1150
    AALWCVGFIG STELGIGLLD NYSLVEDIIE VAYNASVTSV RFTAFYVLGL 1200
    ISMTREGCEI LDEMGWNCCV SVQDEPIGIA LPNRLDRFLS YNEHKWSAFG 1250
    EYSDEMIVFN KSDGDLIEKC LPIEFDLDKL LKEKDTAENP LNEKIITNKY 1300
    DNDITSQTIT VSGENSSLFA NEGLSSPYVT QYRNDDDSIE SKVLHIVSQL 1350
    GNHILSNHAV KEITEINNKY GPRLFENEKM FFKVFNMMSK YRFKPHVRKF 1400
    LCGLFINNRA LENVIRHDNK RDKRPANFTR 1430
    Length:1,430
    Mass (Da):164,368
    Last modified:February 1, 1995 - v1
    Checksum:i07BB5D75BA204E50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64648.1.
    U32508 Genomic DNA. Translation: AAB60298.1.
    BK006939 Genomic DNA. Translation: DAA07754.1.
    PIRiS50596.
    RefSeqiNP_011018.1. NM_001178984.1.

    Genome annotation databases

    EnsemblFungiiYER093C; YER093C; YER093C.
    GeneIDi856828.
    KEGGisce:YER093C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18839 Genomic DNA. Translation: AAB64648.1 .
    U32508 Genomic DNA. Translation: AAB60298.1 .
    BK006939 Genomic DNA. Translation: DAA07754.1 .
    PIRi S50596.
    RefSeqi NP_011018.1. NM_001178984.1.

    3D structure databases

    ProteinModelPortali P40061.
    SMRi P40061. Positions 627-658, 1148-1215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36838. 180 interactions.
    DIPi DIP-4957N.
    IntActi P40061. 10 interactions.
    MINTi MINT-486144.
    STRINGi 4932.YER093C.

    Proteomic databases

    MaxQBi P40061.
    PaxDbi P40061.
    PeptideAtlasi P40061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER093C ; YER093C ; YER093C .
    GeneIDi 856828.
    KEGGi sce:YER093C.

    Organism-specific databases

    CYGDi YER093c.
    SGDi S000000895. TSC11.

    Phylogenomic databases

    eggNOGi NOG305760.
    GeneTreei ENSGT00390000002096.
    HOGENOMi HOG000141815.
    OMAi GRNDTYV.
    OrthoDBi EOG71VT21.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30260-MONOMER.
    Reactomei REACT_188925. CD28 dependent PI3K/Akt signaling.
    REACT_188931. PIP3 activates AKT signaling.
    REACT_223756. Constitutive PI3K/AKT Signaling in Cancer.

    Miscellaneous databases

    NextBioi 983121.

    Gene expression databases

    Genevestigatori P40061.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR028268. Pianissimo_fam.
    IPR028267. Pianissimo_N.
    IPR029453. Rictor_IV.
    IPR029451. RICTOR_M.
    IPR029452. RICTOR_V.
    [Graphical view ]
    PANTHERi PTHR13298. PTHR13298. 1 hit.
    Pfami PF14663. RasGEF_N_2. 1 hit.
    PF14666. RICTOR_M. 1 hit.
    PF14664. RICTOR_N. 1 hit.
    PF14668. RICTOR_V. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 4 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Structure and regulation of MET6, the vitamin B12-independent methionine synthase gene of Saccharomyces cerevisiae."
      Korch C., Mountain H.A., Wenzlau J.M.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 967-1430.
      Strain: ATCC 204508 / S288c.
    4. "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control."
      Loewith R., Jacinto E., Wullschleger S., Lorberg A., Crespo J.L., Bonenfant D., Oppliger W., Jenoe P., Hall M.N.
      Mol. Cell 10:457-468(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae."
      Wedaman K.P., Reinke A., Anderson S., Yates J.R. III, McCaffery J.M., Powers T.
      Mol. Biol. Cell 14:1204-1220(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN TORC2, IDENTIFICATION BY MASS SPECTROMETRY.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Molecular organization of target of rapamycin complex 2."
      Wullschleger S., Loewith R., Oppliger W., Hall M.N.
      J. Biol. Chem. 280:30697-30704(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH TOR2, PHOSPHORYLATION.
    8. "Saccharomyces cerevisiae TSC11/AVO3 participates in regulating cell integrity and functionally interacts with components of the Tor2 complex."
      Ho H.-L., Shiau Y.-S., Chen M.-Y.
      Curr. Genet. 47:273-288(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-81; SER-87 AND SER-141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTSC11_YEAST
    AccessioniPrimary (citable) accession number: P40061
    Secondary accession number(s): D3DM00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1840 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3