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Protein

Transcriptional regulatory protein DOT6

Gene

DOT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the RPD3 histone deacetylase complex RPD3C(L) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. DOT6 binds to sequences containing the core CGATG, which resembles the PAC (Polymerase A and C) motif.2 Publications

Miscellaneous

Present with 2120 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi94 – 117H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • sequence-specific DNA binding Source: SGD

GO - Biological processi

  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at telomere Source: SGD
  • regulation of transcription by RNA polymerase II Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • transfer RNA gene-mediated silencing Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30257-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein DOT6
Alternative name(s):
Disrupter of telomere silencing protein 6
PAC-binding factor 2
Gene namesi
Name:DOT6
Synonyms:PBF2
Ordered Locus Names:YER088C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER088C
SGDiS000000890 DOT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001971081 – 670Transcriptional regulatory protein DOT6Add BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei245PhosphoserineCombined sources1
Modified residuei247PhosphoserineCombined sources1
Modified residuei487PhosphoserineCombined sources1
Modified residuei489PhosphothreonineCombined sources1
Modified residuei491PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40059
PaxDbiP40059
PRIDEiP40059

PTM databases

iPTMnetiP40059

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, DOT6, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, TOD6; UME1 and UME6.1 Publication

Protein-protein interaction databases

BioGridi36833, 104 interactors
DIPiDIP-4823N
IntActiP40059, 7 interactors
MINTiP40059
STRINGi4932.YER088C

Structurei

3D structure databases

ProteinModelPortaliP40059
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 121HTH myb-typePROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Belongs to the DOT6 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000068139
InParanoidiP40059
OMAiKNPSSWD
OrthoDBiEOG092C5EMI

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017930 Myb_dom
IPR001005 SANT/Myb
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS51294 HTH_MYB, 1 hit

Sequencei

Sequence statusi: Complete.

P40059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISTSLNSA SIHLSSMDTH PQLHSLTRQP HSSSTAMSKN EAQESSPSLP
60 70 80 90 100
ASSSSSTSAS ASASSKNSSK NPSSWDPQDD LLLRHLKEVK KMGWKDISQY
110 120 130 140 150
FPNRTPNACQ FRWRRLKSGN LKSNKTALID INTYTGPLKI THGDETANAQ
160 170 180 190 200
QKPSKKVEEN VLTEDTAEFT TTSSIPIPSR KTSLPSFHAS MSFSQSPSNV
210 220 230 240 250
TPTTIVSNAA SSMPFAPPTL PAALPHHPHQ HLHHHPHHKT LKPRSNSHSF
260 270 280 290 300
TNSLNQDPIV RSNDEEKYGF IPKVFVRSRR SSFAYPQQVA ITTTPSSPNS
310 320 330 340 350
SHVLLSSKSR RGSLANWSRR SSFNVSSNNT SRRSSMILAP NSVSNIFNVN
360 370 380 390 400
NSGSNTASTS NTNSRRESVI KKEFQQRLNN LSNSGGPTSN NGPIFPNSYT
410 420 430 440 450
FMDLPHSSSV SSSSTLHKSK RGSFSGHSMK SSCNPTNLWS KDEDALLMEN
460 470 480 490 500
KKRNLSVMEL SILLPQRTEV EIQWRLNALS SDADMLSPTH SPQKTLSKKT
510 520 530 540 550
CPRMFKSGST TDDDKGSDKE DVMGDGSNDD DEDNVDPLHR AKQSSNKTVF
560 570 580 590 600
SSSSSNISSK DVSPDPIFSP DPADDSSNTS DAGSRCTITS DTSSSAATMN
610 620 630 640 650
RTPNSKNPQD IALLNNFRSE AITPRPKPSS TTTSITTETT NNMINHSSST
660 670
TTTTNNSPLP SINTIFKDML
Length:670
Mass (Da):73,048
Last modified:February 1, 1995 - v1
Checksum:i61FD64749653FE78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA Translation: AAB64643.1
BK006939 Genomic DNA Translation: DAA07749.1
PIRiS50591
RefSeqiNP_011012.3, NM_001178979.3

Genome annotation databases

EnsemblFungiiYER088C; YER088C; YER088C
GeneIDi856822
KEGGisce:YER088C

Similar proteinsi

Entry informationi

Entry nameiDOT6_YEAST
AccessioniPrimary (citable) accession number: P40059
Secondary accession number(s): D3DLZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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