Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcriptional regulatory protein DOT6

Gene

DOT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the RPD3 histone deacetylase complex RPD3C(L) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. DOT6 binds to sequences containing the core CGATG, which resembles the PAC (Polymerase A and C) motif.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi94 – 11724H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: SGD

GO - Biological processi

  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at telomere Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • transfer RNA gene-mediated silencing Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30257-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein DOT6
Alternative name(s):
Disrupter of telomere silencing protein 6
PAC-binding factor 2
Gene namesi
Name:DOT6
Synonyms:PBF2
Ordered Locus Names:YER088C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER088C.
SGDiS000000890. DOT6.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670Transcriptional regulatory protein DOT6PRO_0000197108Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei245 – 2451PhosphoserineCombined sources
Modified residuei247 – 2471PhosphoserineCombined sources
Modified residuei487 – 4871PhosphoserineCombined sources
Modified residuei489 – 4891PhosphothreonineCombined sources
Modified residuei491 – 4911PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40059.
PeptideAtlasiP40059.

PTM databases

iPTMnetiP40059.

Interactioni

Subunit structurei

Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, DOT6, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, TOD6; UME1 and UME6.1 Publication

Protein-protein interaction databases

BioGridi36833. 65 interactions.
DIPiDIP-4823N.
IntActiP40059. 7 interactions.
MINTiMINT-527937.

Structurei

3D structure databases

ProteinModelPortaliP40059.
SMRiP40059. Positions 73-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 12155HTH myb-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DOT6 family.Curated
Contains 1 HTH myb-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000068139.
InParanoidiP40059.
OMAiKNPSSWD.
OrthoDBiEOG7C2RCB.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51294. HTH_MYB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISTSLNSA SIHLSSMDTH PQLHSLTRQP HSSSTAMSKN EAQESSPSLP
60 70 80 90 100
ASSSSSTSAS ASASSKNSSK NPSSWDPQDD LLLRHLKEVK KMGWKDISQY
110 120 130 140 150
FPNRTPNACQ FRWRRLKSGN LKSNKTALID INTYTGPLKI THGDETANAQ
160 170 180 190 200
QKPSKKVEEN VLTEDTAEFT TTSSIPIPSR KTSLPSFHAS MSFSQSPSNV
210 220 230 240 250
TPTTIVSNAA SSMPFAPPTL PAALPHHPHQ HLHHHPHHKT LKPRSNSHSF
260 270 280 290 300
TNSLNQDPIV RSNDEEKYGF IPKVFVRSRR SSFAYPQQVA ITTTPSSPNS
310 320 330 340 350
SHVLLSSKSR RGSLANWSRR SSFNVSSNNT SRRSSMILAP NSVSNIFNVN
360 370 380 390 400
NSGSNTASTS NTNSRRESVI KKEFQQRLNN LSNSGGPTSN NGPIFPNSYT
410 420 430 440 450
FMDLPHSSSV SSSSTLHKSK RGSFSGHSMK SSCNPTNLWS KDEDALLMEN
460 470 480 490 500
KKRNLSVMEL SILLPQRTEV EIQWRLNALS SDADMLSPTH SPQKTLSKKT
510 520 530 540 550
CPRMFKSGST TDDDKGSDKE DVMGDGSNDD DEDNVDPLHR AKQSSNKTVF
560 570 580 590 600
SSSSSNISSK DVSPDPIFSP DPADDSSNTS DAGSRCTITS DTSSSAATMN
610 620 630 640 650
RTPNSKNPQD IALLNNFRSE AITPRPKPSS TTTSITTETT NNMINHSSST
660 670
TTTTNNSPLP SINTIFKDML
Length:670
Mass (Da):73,048
Last modified:February 1, 1995 - v1
Checksum:i61FD64749653FE78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64643.1.
BK006939 Genomic DNA. Translation: DAA07749.1.
PIRiS50591.
RefSeqiNP_011012.3. NM_001178979.3.

Genome annotation databases

EnsemblFungiiYER088C; YER088C; YER088C.
GeneIDi856822.
KEGGisce:YER088C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18839 Genomic DNA. Translation: AAB64643.1.
BK006939 Genomic DNA. Translation: DAA07749.1.
PIRiS50591.
RefSeqiNP_011012.3. NM_001178979.3.

3D structure databases

ProteinModelPortaliP40059.
SMRiP40059. Positions 73-115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36833. 65 interactions.
DIPiDIP-4823N.
IntActiP40059. 7 interactions.
MINTiMINT-527937.

PTM databases

iPTMnetiP40059.

Proteomic databases

MaxQBiP40059.
PeptideAtlasiP40059.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER088C; YER088C; YER088C.
GeneIDi856822.
KEGGisce:YER088C.

Organism-specific databases

EuPathDBiFungiDB:YER088C.
SGDiS000000890. DOT6.

Phylogenomic databases

GeneTreeiENSGT00530000068139.
InParanoidiP40059.
OMAiKNPSSWD.
OrthoDBiEOG7C2RCB.

Enzyme and pathway databases

BioCyciYEAST:G3O-30257-MONOMER.

Miscellaneous databases

NextBioi983106.
PROiP40059.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51294. HTH_MYB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae."
    Singer M.S., Kahana A., Wolf A.J., Meisinger L.L., Peterson S.E., Goggin C., Mahowald M., Gottschling D.E.
    Genetics 150:613-632(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment."
    Shevchenko A., Roguev A., Schaft D., Buchanan L., Habermann B., Sakalar C., Thomas H., Krogan N.J., Shevchenko A., Stewart A.F.
    Genome Biol. 9:R167.1-R167.22(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RPD3(L) COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: DNA-BINDING.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: FUNCTION.
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245; SER-247; SER-487; THR-489 AND SER-491, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDOT6_YEAST
AccessioniPrimary (citable) accession number: P40059
Secondary accession number(s): D3DLZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2120 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.